Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5452 and Pf1N1B4_5453 are separated by 30 nucleotides Pf1N1B4_5453 and Pf1N1B4_5454 are separated by 704 nucleotides
Pf1N1B4_5452 - hypothetical protein, at 199,278 to 199,538
_5452
Pf1N1B4_5453 - hypothetical protein, at 199,569 to 199,898
_5453
Pf1N1B4_5454 - hypothetical protein, at 200,603 to 200,998
_5454
Position (kb)
199
200 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 198.635 kb on + strand at 198.636 kb on - strand at 198.636 kb on - strand at 198.661 kb on + strand at 198.709 kb on + strand at 198.709 kb on + strand at 198.793 kb on + strand at 198.793 kb on + strand at 198.793 kb on + strand at 198.793 kb on + strand at 198.793 kb on + strand at 198.794 kb on - strand at 198.895 kb on - strand at 198.981 kb on + strand at 198.982 kb on - strand at 198.982 kb on - strand at 199.101 kb on + strand at 199.101 kb on + strand at 199.101 kb on + strand at 199.130 kb on + strand at 199.130 kb on + strand at 199.130 kb on + strand at 199.148 kb on + strand at 199.148 kb on + strand at 199.148 kb on + strand at 199.149 kb on - strand at 199.163 kb on + strand at 199.163 kb on + strand at 199.187 kb on + strand at 199.187 kb on + strand at 199.187 kb on + strand at 199.189 kb on + strand at 199.189 kb on + strand at 199.213 kb on + strand at 199.239 kb on + strand at 199.249 kb on - strand at 199.249 kb on - strand at 199.354 kb on + strand at 199.355 kb on - strand, within Pf1N1B4_5452 at 199.355 kb on - strand, within Pf1N1B4_5452 at 199.366 kb on - strand, within Pf1N1B4_5452 at 199.367 kb on + strand, within Pf1N1B4_5452 at 199.367 kb on + strand, within Pf1N1B4_5452 at 199.367 kb on + strand, within Pf1N1B4_5452 at 199.436 kb on + strand, within Pf1N1B4_5452 at 199.437 kb on - strand, within Pf1N1B4_5452 at 199.437 kb on - strand, within Pf1N1B4_5452 at 199.437 kb on - strand, within Pf1N1B4_5452 at 199.469 kb on - strand, within Pf1N1B4_5452 at 199.469 kb on - strand, within Pf1N1B4_5452 at 199.473 kb on + strand, within Pf1N1B4_5452 at 199.474 kb on - strand, within Pf1N1B4_5452 at 199.539 kb on + strand at 199.576 kb on - strand at 199.670 kb on - strand, within Pf1N1B4_5453 at 199.830 kb on - strand, within Pf1N1B4_5453 at 199.830 kb on - strand, within Pf1N1B4_5453 at 199.830 kb on - strand, within Pf1N1B4_5453 at 199.840 kb on + strand, within Pf1N1B4_5453 at 199.841 kb on - strand, within Pf1N1B4_5453 at 199.841 kb on - strand, within Pf1N1B4_5453 at 199.841 kb on - strand, within Pf1N1B4_5453 at 199.844 kb on + strand, within Pf1N1B4_5453 at 199.845 kb on - strand, within Pf1N1B4_5453 at 199.888 kb on - strand at 199.896 kb on + strand at 199.896 kb on + strand at 199.896 kb on + strand at 199.896 kb on + strand at 200.046 kb on - strand at 200.249 kb on - strand at 200.263 kb on + strand at 200.264 kb on - strand at 200.264 kb on - strand at 200.286 kb on + strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.287 kb on - strand at 200.314 kb on + strand at 200.314 kb on + strand at 200.314 kb on + strand at 200.314 kb on + strand at 200.314 kb on + strand at 200.314 kb on + strand at 200.314 kb on + strand at 200.315 kb on - strand at 200.315 kb on - strand at 200.315 kb on - strand at 200.315 kb on - strand at 200.363 kb on + strand at 200.363 kb on + strand at 200.363 kb on + strand at 200.395 kb on + strand at 200.416 kb on + strand at 200.416 kb on + strand at 200.416 kb on + strand at 200.416 kb on + strand at 200.416 kb on + strand at 200.416 kb on + strand at 200.416 kb on + strand at 200.442 kb on - strand at 200.496 kb on + strand at 200.496 kb on + strand at 200.496 kb on + strand at 200.497 kb on - strand at 200.497 kb on - strand at 200.604 kb on + strand at 200.609 kb on - strand at 200.699 kb on + strand, within Pf1N1B4_5454 at 200.700 kb on - strand, within Pf1N1B4_5454 at 200.753 kb on + strand, within Pf1N1B4_5454 at 200.753 kb on + strand at 200.753 kb on + strand, within Pf1N1B4_5454 at 200.754 kb on - strand, within Pf1N1B4_5454 at 200.754 kb on - strand, within Pf1N1B4_5454 at 200.754 kb on - strand, within Pf1N1B4_5454 at 200.769 kb on + strand, within Pf1N1B4_5454 at 200.770 kb on - strand, within Pf1N1B4_5454 at 200.770 kb on - strand, within Pf1N1B4_5454 at 200.824 kb on - strand, within Pf1N1B4_5454 at 200.824 kb on - strand, within Pf1N1B4_5454
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 198,635 + -1.2 198,636 - -0.6 198,636 - -1.3 198,661 + +0.2 198,709 + +1.6 198,709 + -1.3 198,793 + -0.4 198,793 + -1.1 198,793 + -0.1 198,793 + -0.5 198,793 + -1.3 198,794 - -1.8 198,895 - -0.1 198,981 + +0.3 198,982 - +0.4 198,982 - -0.1 199,101 + +2.7 199,101 + +0.7 199,101 + +0.2 199,130 + +0.1 199,130 + +0.3 199,130 + +0.5 199,148 + +0.2 199,148 + -0.4 199,148 + +0.5 199,149 - -1.3 199,163 + +0.4 199,163 + -0.9 199,187 + +1.4 199,187 + +0.0 199,187 + -0.3 199,189 + +0.9 199,189 + -1.0 199,213 + -0.3 199,239 + +0.2 199,249 - -1.0 199,249 - +0.2 199,354 + +2.3 199,355 - Pf1N1B4_5452 0.30 -0.1 199,355 - Pf1N1B4_5452 0.30 +0.3 199,366 - Pf1N1B4_5452 0.34 -0.4 199,367 + Pf1N1B4_5452 0.34 +0.1 199,367 + Pf1N1B4_5452 0.34 -0.4 199,367 + Pf1N1B4_5452 0.34 -0.6 199,436 + Pf1N1B4_5452 0.61 -0.3 199,437 - Pf1N1B4_5452 0.61 +1.0 199,437 - Pf1N1B4_5452 0.61 +1.1 199,437 - Pf1N1B4_5452 0.61 -0.1 199,469 - Pf1N1B4_5452 0.73 +2.0 199,469 - Pf1N1B4_5452 0.73 -0.4 199,473 + Pf1N1B4_5452 0.75 +0.2 199,474 - Pf1N1B4_5452 0.75 -1.0 199,539 + -0.5 199,576 - +0.3 199,670 - Pf1N1B4_5453 0.31 -0.6 199,830 - Pf1N1B4_5453 0.79 -1.4 199,830 - Pf1N1B4_5453 0.79 +0.5 199,830 - Pf1N1B4_5453 0.79 +0.7 199,840 + Pf1N1B4_5453 0.82 -0.3 199,841 - Pf1N1B4_5453 0.82 -0.1 199,841 - Pf1N1B4_5453 0.82 -1.6 199,841 - Pf1N1B4_5453 0.82 -0.6 199,844 + Pf1N1B4_5453 0.83 -1.1 199,845 - Pf1N1B4_5453 0.84 -2.1 199,888 - +0.2 199,896 + -0.1 199,896 + -1.0 199,896 + -2.4 199,896 + +0.5 200,046 - +0.1 200,249 - +0.7 200,263 + -0.3 200,264 - +0.7 200,264 - -1.6 200,286 + -1.2 200,287 - +0.7 200,287 - +0.0 200,287 - -0.3 200,287 - -0.3 200,287 - -1.0 200,287 - +0.7 200,287 - +1.2 200,287 - -0.8 200,287 - -0.7 200,314 + -0.4 200,314 + -0.1 200,314 + +0.7 200,314 + -1.3 200,314 + +0.4 200,314 + -0.3 200,314 + +0.2 200,315 - +1.0 200,315 - +0.6 200,315 - -0.5 200,315 - +0.7 200,363 + -0.4 200,363 + +3.3 200,363 + -0.3 200,395 + +0.7 200,416 + -1.8 200,416 + -0.5 200,416 + -0.6 200,416 + -0.3 200,416 + +0.2 200,416 + -1.1 200,416 + +0.3 200,442 - -1.1 200,496 + -0.7 200,496 + -0.9 200,496 + -0.8 200,497 - +1.3 200,497 - -1.3 200,604 + -1.8 200,609 - -0.6 200,699 + Pf1N1B4_5454 0.24 +0.0 200,700 - Pf1N1B4_5454 0.24 +0.6 200,753 + Pf1N1B4_5454 0.38 +1.1 200,753 + +1.7 200,753 + Pf1N1B4_5454 0.38 -1.8 200,754 - Pf1N1B4_5454 0.38 +0.8 200,754 - Pf1N1B4_5454 0.38 +1.3 200,754 - Pf1N1B4_5454 0.38 +0.4 200,769 + Pf1N1B4_5454 0.42 -1.7 200,770 - Pf1N1B4_5454 0.42 -0.1 200,770 - Pf1N1B4_5454 0.42 -1.1 200,824 - Pf1N1B4_5454 0.56 +0.7 200,824 - Pf1N1B4_5454 0.56 -0.1
Or see this region's nucleotide sequence