Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5401 and Pf1N1B4_5402 overlap by 1 nucleotides
Pf1N1B4_5401 - hypothetical protein, at 134,226 to 135,608
_5401
Pf1N1B4_5402 - hypothetical protein, at 135,608 to 135,892
_5402
Position (kb)
135
136 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 134.698 kb on + strand, within Pf1N1B4_5401 at 134.699 kb on - strand, within Pf1N1B4_5401 at 134.699 kb on - strand, within Pf1N1B4_5401 at 134.863 kb on + strand, within Pf1N1B4_5401 at 134.864 kb on - strand, within Pf1N1B4_5401 at 134.900 kb on - strand, within Pf1N1B4_5401 at 135.039 kb on + strand, within Pf1N1B4_5401 at 135.040 kb on - strand, within Pf1N1B4_5401 at 135.043 kb on + strand, within Pf1N1B4_5401 at 135.043 kb on + strand, within Pf1N1B4_5401 at 135.074 kb on + strand, within Pf1N1B4_5401 at 135.099 kb on + strand, within Pf1N1B4_5401 at 135.100 kb on - strand, within Pf1N1B4_5401 at 135.100 kb on - strand, within Pf1N1B4_5401 at 135.119 kb on - strand, within Pf1N1B4_5401 at 135.430 kb on + strand, within Pf1N1B4_5401 at 135.430 kb on + strand, within Pf1N1B4_5401 at 135.430 kb on + strand, within Pf1N1B4_5401 at 135.431 kb on - strand, within Pf1N1B4_5401 at 135.431 kb on - strand, within Pf1N1B4_5401 at 135.448 kb on + strand, within Pf1N1B4_5401 at 135.449 kb on - strand, within Pf1N1B4_5401 at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.482 kb on + strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.483 kb on - strand at 135.491 kb on - strand at 135.491 kb on - strand at 135.491 kb on - strand at 135.625 kb on + strand at 135.625 kb on + strand at 135.625 kb on + strand at 135.626 kb on - strand at 135.631 kb on - strand at 135.692 kb on + strand, within Pf1N1B4_5402 at 135.692 kb on + strand, within Pf1N1B4_5402 at 135.693 kb on - strand at 135.693 kb on - strand, within Pf1N1B4_5402 at 135.693 kb on - strand, within Pf1N1B4_5402 at 135.748 kb on + strand, within Pf1N1B4_5402 at 135.808 kb on + strand at 135.809 kb on - strand, within Pf1N1B4_5402 at 135.809 kb on - strand, within Pf1N1B4_5402 at 135.919 kb on - strand at 135.940 kb on - strand at 135.994 kb on - strand at 135.994 kb on - strand at 135.994 kb on - strand at 136.011 kb on - strand at 136.011 kb on - strand at 136.050 kb on + strand at 136.050 kb on + strand at 136.051 kb on - strand at 136.051 kb on - strand at 136.138 kb on + strand at 136.139 kb on - strand at 136.261 kb on - strand at 136.261 kb on - strand at 136.307 kb on + strand at 136.307 kb on + strand at 136.308 kb on - strand at 136.308 kb on - strand at 136.308 kb on - strand at 136.308 kb on - strand at 136.309 kb on + strand at 136.353 kb on + strand at 136.448 kb on + strand at 136.449 kb on - strand at 136.449 kb on - strand at 136.449 kb on - strand at 136.449 kb on - strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.555 kb on + strand at 136.556 kb on - strand at 136.556 kb on - strand at 136.556 kb on - strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.559 kb on + strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.560 kb on - strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.768 kb on + strand at 136.769 kb on - strand at 136.769 kb on - strand at 136.861 kb on + strand at 136.861 kb on + strand at 136.862 kb on - strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.888 kb on + strand at 136.889 kb on - strand at 136.889 kb on - strand at 136.889 kb on - strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand at 136.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 134,698 + Pf1N1B4_5401 0.34 +0.7 134,699 - Pf1N1B4_5401 0.34 -0.9 134,699 - Pf1N1B4_5401 0.34 -0.4 134,863 + Pf1N1B4_5401 0.46 -0.8 134,864 - Pf1N1B4_5401 0.46 -0.8 134,900 - Pf1N1B4_5401 0.49 +1.0 135,039 + Pf1N1B4_5401 0.59 -0.3 135,040 - Pf1N1B4_5401 0.59 -0.8 135,043 + Pf1N1B4_5401 0.59 +1.1 135,043 + Pf1N1B4_5401 0.59 -0.3 135,074 + Pf1N1B4_5401 0.61 -1.1 135,099 + Pf1N1B4_5401 0.63 -0.3 135,100 - Pf1N1B4_5401 0.63 -0.2 135,100 - Pf1N1B4_5401 0.63 -0.1 135,119 - Pf1N1B4_5401 0.65 -2.6 135,430 + Pf1N1B4_5401 0.87 +0.7 135,430 + Pf1N1B4_5401 0.87 -1.8 135,430 + Pf1N1B4_5401 0.87 +2.1 135,431 - Pf1N1B4_5401 0.87 +0.4 135,431 - Pf1N1B4_5401 0.87 +0.2 135,448 + Pf1N1B4_5401 0.88 +0.3 135,449 - Pf1N1B4_5401 0.88 -0.1 135,482 + -1.1 135,482 + +0.0 135,482 + -0.3 135,482 + +0.5 135,482 + -1.1 135,482 + +0.3 135,482 + -1.1 135,483 - -1.0 135,483 - -0.0 135,483 - +1.4 135,483 - -0.3 135,483 - -0.8 135,491 - -0.1 135,491 - +0.2 135,491 - +0.2 135,625 + +1.0 135,625 + -0.3 135,625 + -0.7 135,626 - +1.1 135,631 - +1.2 135,692 + Pf1N1B4_5402 0.29 -0.5 135,692 + Pf1N1B4_5402 0.29 -0.5 135,693 - +1.5 135,693 - Pf1N1B4_5402 0.30 -2.1 135,693 - Pf1N1B4_5402 0.30 +0.3 135,748 + Pf1N1B4_5402 0.49 -1.3 135,808 + +1.7 135,809 - Pf1N1B4_5402 0.71 -1.6 135,809 - Pf1N1B4_5402 0.71 -0.3 135,919 - +1.7 135,940 - +0.7 135,994 - +0.5 135,994 - -1.3 135,994 - -0.3 136,011 - +0.2 136,011 - -0.5 136,050 + +0.7 136,050 + -1.6 136,051 - +1.7 136,051 - +0.2 136,138 + +1.2 136,139 - +0.0 136,261 - -0.3 136,261 - +0.0 136,307 + +0.2 136,307 + +0.7 136,308 - +0.7 136,308 - +0.7 136,308 - +0.7 136,308 - +0.3 136,309 + -0.3 136,353 + +0.4 136,448 + -0.6 136,449 - -1.1 136,449 - -0.1 136,449 - -0.8 136,449 - -2.4 136,555 + +1.7 136,555 + +1.7 136,555 + +0.5 136,555 + -1.6 136,555 + +0.0 136,555 + -0.3 136,555 + -1.0 136,556 - -1.6 136,556 - -0.3 136,556 - +0.7 136,559 + -1.3 136,559 + +0.4 136,559 + -0.8 136,559 + -1.2 136,559 + -1.0 136,559 + -0.5 136,559 + +0.3 136,559 + +1.3 136,559 + +0.3 136,559 + -0.8 136,560 - +1.4 136,560 - -0.3 136,560 - -0.6 136,560 - +0.0 136,560 - +0.2 136,560 - +0.3 136,560 - +0.2 136,560 - +0.1 136,768 + +0.3 136,768 + +0.7 136,768 + -0.0 136,768 + +0.4 136,768 + +2.3 136,769 - +0.7 136,769 - -1.6 136,861 + +1.3 136,861 + -1.3 136,862 - -0.8 136,888 + -0.3 136,888 + +2.4 136,888 + -0.0 136,888 + +1.2 136,888 + -1.1 136,888 + -1.7 136,888 + -1.1 136,888 + +0.0 136,888 + +0.7 136,888 + -0.1 136,889 - +0.6 136,889 - +0.0 136,889 - +0.7 136,892 + -0.6 136,892 + -0.5 136,892 + +0.2 136,892 + -0.3 136,892 + -0.8 136,892 + -1.8 136,892 + -0.3 136,892 + -0.3
Or see this region's nucleotide sequence