Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5346

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5344 and Pf1N1B4_5345 overlap by 4 nucleotidesPf1N1B4_5345 and Pf1N1B4_5346 are separated by 80 nucleotidesPf1N1B4_5346 and Pf1N1B4_5347 are separated by 136 nucleotidesPf1N1B4_5347 and Pf1N1B4_5348 are separated by 101 nucleotides Pf1N1B4_5344 - hypothetical protein, at 86,092 to 86,823 _5344 Pf1N1B4_5345 - hypothetical protein, at 86,820 to 87,074 _5345 Pf1N1B4_5346 - hypothetical protein, at 87,155 to 87,868 _5346 Pf1N1B4_5347 - hypothetical protein, at 88,005 to 88,352 _5347 Pf1N1B4_5348 - hypothetical protein, at 88,454 to 88,576 _5348 Position (kb) 87 88Strain fitness (log2 ratio) -2 -1 0 1 2 3at 86.352 kb on + strand, within Pf1N1B4_5344at 86.352 kb on + strand, within Pf1N1B4_5344at 86.352 kb on + strand, within Pf1N1B4_5344at 86.353 kb on - strand, within Pf1N1B4_5344at 86.353 kb on - strand, within Pf1N1B4_5344at 86.353 kb on - strand, within Pf1N1B4_5344at 86.353 kb on - strand, within Pf1N1B4_5344at 86.369 kb on + strandat 86.369 kb on + strand, within Pf1N1B4_5344at 86.369 kb on + strand, within Pf1N1B4_5344at 86.369 kb on + strand, within Pf1N1B4_5344at 86.370 kb on - strand, within Pf1N1B4_5344at 86.370 kb on - strand, within Pf1N1B4_5344at 86.370 kb on - strand, within Pf1N1B4_5344at 86.370 kb on - strand, within Pf1N1B4_5344at 86.444 kb on + strand, within Pf1N1B4_5344at 86.444 kb on + strand, within Pf1N1B4_5344at 86.444 kb on + strand, within Pf1N1B4_5344at 86.445 kb on - strand, within Pf1N1B4_5344at 86.445 kb on - strand, within Pf1N1B4_5344at 86.557 kb on + strand, within Pf1N1B4_5344at 86.557 kb on + strand, within Pf1N1B4_5344at 86.557 kb on + strand, within Pf1N1B4_5344at 86.558 kb on - strand, within Pf1N1B4_5344at 86.571 kb on + strand, within Pf1N1B4_5344at 86.572 kb on - strandat 86.658 kb on - strand, within Pf1N1B4_5344at 86.696 kb on + strand, within Pf1N1B4_5344at 86.737 kb on + strand, within Pf1N1B4_5344at 86.827 kb on + strandat 86.827 kb on + strandat 86.909 kb on - strand, within Pf1N1B4_5345at 87.088 kb on + strandat 87.156 kb on + strandat 87.156 kb on + strandat 87.156 kb on + strandat 87.207 kb on + strandat 87.207 kb on + strandat 87.207 kb on + strandat 87.207 kb on + strandat 87.208 kb on - strandat 87.208 kb on - strandat 87.208 kb on - strandat 87.208 kb on - strandat 87.208 kb on - strandat 87.208 kb on - strandat 87.208 kb on - strandat 87.401 kb on - strand, within Pf1N1B4_5346at 87.401 kb on - strand, within Pf1N1B4_5346at 87.401 kb on - strand, within Pf1N1B4_5346at 87.401 kb on - strand, within Pf1N1B4_5346at 87.401 kb on - strand, within Pf1N1B4_5346at 87.401 kb on - strandat 87.401 kb on - strand, within Pf1N1B4_5346at 87.401 kb on - strand, within Pf1N1B4_5346at 87.401 kb on - strandat 87.401 kb on - strandat 87.597 kb on + strand, within Pf1N1B4_5346at 87.835 kb on + strandat 87.914 kb on + strandat 87.914 kb on + strandat 87.914 kb on + strandat 87.915 kb on - strandat 87.920 kb on + strandat 87.986 kb on - strandat 88.016 kb on - strandat 88.495 kb on - strand, within Pf1N1B4_5348at 88.495 kb on - strand, within Pf1N1B4_5348at 88.495 kb on - strand, within Pf1N1B4_5348at 88.560 kb on - strandat 88.560 kb on - strand, within Pf1N1B4_5348at 88.642 kb on - strandat 88.671 kb on + strandat 88.672 kb on - strandat 88.793 kb on - strandat 88.803 kb on + strandat 88.804 kb on - strandat 88.859 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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86,352 + Pf1N1B4_5344 0.36 +0.3
86,352 + Pf1N1B4_5344 0.36 -0.8
86,352 + Pf1N1B4_5344 0.36 -2.3
86,353 - Pf1N1B4_5344 0.36 -0.3
86,353 - Pf1N1B4_5344 0.36 +0.2
86,353 - Pf1N1B4_5344 0.36 +0.0
86,353 - Pf1N1B4_5344 0.36 +0.0
86,369 + -0.8
86,369 + Pf1N1B4_5344 0.38 +0.3
86,369 + Pf1N1B4_5344 0.38 +0.7
86,369 + Pf1N1B4_5344 0.38 +1.1
86,370 - Pf1N1B4_5344 0.38 -0.6
86,370 - Pf1N1B4_5344 0.38 +0.5
86,370 - Pf1N1B4_5344 0.38 +0.0
86,370 - Pf1N1B4_5344 0.38 +0.0
86,444 + Pf1N1B4_5344 0.48 -1.1
86,444 + Pf1N1B4_5344 0.48 +0.7
86,444 + Pf1N1B4_5344 0.48 +1.0
86,445 - Pf1N1B4_5344 0.48 +0.2
86,445 - Pf1N1B4_5344 0.48 -0.4
86,557 + Pf1N1B4_5344 0.64 -0.8
86,557 + Pf1N1B4_5344 0.64 -0.6
86,557 + Pf1N1B4_5344 0.64 -1.3
86,558 - Pf1N1B4_5344 0.64 +0.5
86,571 + Pf1N1B4_5344 0.65 +0.4
86,572 - +0.2
86,658 - Pf1N1B4_5344 0.77 -0.4
86,696 + Pf1N1B4_5344 0.83 -0.6
86,737 + Pf1N1B4_5344 0.88 +1.5
86,827 + +0.0
86,827 + +0.4
86,909 - Pf1N1B4_5345 0.35 -0.5
87,088 + +0.5
87,156 + -0.1
87,156 + -1.1
87,156 + -0.6
87,207 + -2.1
87,207 + -1.6
87,207 + -1.1
87,207 + +0.4
87,208 - -0.4
87,208 - +1.0
87,208 - +0.3
87,208 - +0.7
87,208 - -0.3
87,208 - -0.3
87,208 - +3.3
87,401 - Pf1N1B4_5346 0.34 -1.1
87,401 - Pf1N1B4_5346 0.34 +0.2
87,401 - Pf1N1B4_5346 0.34 -1.6
87,401 - Pf1N1B4_5346 0.34 -1.3
87,401 - Pf1N1B4_5346 0.34 -1.1
87,401 - +1.5
87,401 - Pf1N1B4_5346 0.34 -1.1
87,401 - Pf1N1B4_5346 0.34 +0.2
87,401 - +1.3
87,401 - +0.2
87,597 + Pf1N1B4_5346 0.62 +0.4
87,835 + -0.8
87,914 + +0.7
87,914 + +0.7
87,914 + -0.8
87,915 - -0.3
87,920 + -0.1
87,986 - +1.7
88,016 - -1.3
88,495 - Pf1N1B4_5348 0.33 -0.1
88,495 - Pf1N1B4_5348 0.33 +0.4
88,495 - Pf1N1B4_5348 0.33 -0.3
88,560 - -0.8
88,560 - Pf1N1B4_5348 0.86 -0.1
88,642 - +1.2
88,671 + +1.7
88,672 - -0.8
88,793 - +0.1
88,803 + -0.1
88,804 - -0.2
88,859 - -1.6

Or see this region's nucleotide sequence