Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5333 and Pf1N1B4_5334 are separated by 131 nucleotides Pf1N1B4_5334 and Pf1N1B4_5335 are separated by 181 nucleotides Pf1N1B4_5335 and Pf1N1B4_5336 are separated by 448 nucleotides
Pf1N1B4_5333 - hypothetical protein, at 78,969 to 79,595
_5333
Pf1N1B4_5334 - hypothetical protein, at 79,727 to 79,930
_5334
Pf1N1B4_5335 - YebG, DNA damage-inducible gene in SOS regulon, expressed in stationary phase, at 80,112 to 80,399
_5335
Pf1N1B4_5336 - hypothetical protein, at 80,848 to 81,201
_5336
Position (kb)
80
81 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.446 kb on - strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.580 kb on + strand at 79.580 kb on + strand at 79.726 kb on - strand at 79.728 kb on + strand at 79.793 kb on + strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.797 kb on + strand, within Pf1N1B4_5334 at 80.045 kb on + strand at 80.045 kb on + strand at 80.045 kb on + strand at 80.046 kb on - strand at 80.147 kb on + strand, within Pf1N1B4_5335 at 80.147 kb on + strand, within Pf1N1B4_5335 at 80.148 kb on - strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.228 kb on - strand at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.348 kb on + strand, within Pf1N1B4_5335 at 80.348 kb on + strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.380 kb on + strand at 80.584 kb on - strand at 80.628 kb on + strand at 80.628 kb on + strand at 80.629 kb on - strand at 80.629 kb on - strand at 80.629 kb on - strand at 80.629 kb on - strand at 80.695 kb on + strand at 80.696 kb on - strand at 80.696 kb on - strand at 80.696 kb on - strand at 80.696 kb on - strand at 80.707 kb on - strand at 80.707 kb on - strand at 80.817 kb on + strand at 80.817 kb on + strand at 80.857 kb on + strand at 80.858 kb on - strand at 81.059 kb on - strand, within Pf1N1B4_5336 at 81.061 kb on + strand at 81.329 kb on + strand at 81.329 kb on + strand at 81.329 kb on + strand at 81.329 kb on + strand at 81.330 kb on - strand at 81.330 kb on - strand at 81.330 kb on - strand at 81.330 kb on - strand at 81.330 kb on - strand at 81.333 kb on + strand at 81.333 kb on + strand at 81.333 kb on + strand at 81.334 kb on - strand at 81.334 kb on - strand at 81.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 79,268 + Pf1N1B4_5333 0.48 -0.3 79,268 + Pf1N1B4_5333 0.48 -1.6 79,269 - Pf1N1B4_5333 0.48 -0.1 79,269 - Pf1N1B4_5333 0.48 -0.6 79,393 - Pf1N1B4_5333 0.68 +1.7 79,393 - Pf1N1B4_5333 0.68 +0.3 79,446 - Pf1N1B4_5333 0.76 -0.5 79,501 + Pf1N1B4_5333 0.85 +0.5 79,501 + Pf1N1B4_5333 0.85 -0.3 79,502 - Pf1N1B4_5333 0.85 +0.3 79,502 - Pf1N1B4_5333 0.85 -0.8 79,576 + -0.1 79,576 + -2.3 79,576 + +0.2 79,576 + -0.8 79,577 - -0.8 79,577 - -1.6 79,577 - -0.5 79,577 - -0.6 79,577 - -0.8 79,580 + -0.4 79,580 + +0.2 79,726 - +1.7 79,728 + +1.5 79,793 + Pf1N1B4_5334 0.32 +1.4 79,794 - Pf1N1B4_5334 0.33 +0.5 79,794 - Pf1N1B4_5334 0.33 +0.7 79,794 - Pf1N1B4_5334 0.33 +0.5 79,794 - Pf1N1B4_5334 0.33 +0.0 79,797 + Pf1N1B4_5334 0.34 -0.8 80,045 + -0.5 80,045 + +1.7 80,045 + -1.6 80,046 - -0.8 80,147 + Pf1N1B4_5335 0.12 +2.1 80,147 + Pf1N1B4_5335 0.12 +1.7 80,148 - Pf1N1B4_5335 0.12 +0.5 80,227 + Pf1N1B4_5335 0.40 +0.2 80,227 + +1.7 80,227 + Pf1N1B4_5335 0.40 +0.4 80,227 + Pf1N1B4_5335 0.40 -0.6 80,227 + Pf1N1B4_5335 0.40 +1.7 80,227 + Pf1N1B4_5335 0.40 -1.0 80,227 + Pf1N1B4_5335 0.40 +0.7 80,227 + Pf1N1B4_5335 0.40 +1.5 80,227 + Pf1N1B4_5335 0.40 +0.9 80,227 + Pf1N1B4_5335 0.40 -0.3 80,227 + Pf1N1B4_5335 0.40 -0.7 80,228 - Pf1N1B4_5335 0.40 +1.1 80,228 - Pf1N1B4_5335 0.40 +0.3 80,228 - +1.2 80,228 - Pf1N1B4_5335 0.40 +1.6 80,348 + Pf1N1B4_5335 0.82 +0.2 80,348 + Pf1N1B4_5335 0.82 +0.5 80,349 - Pf1N1B4_5335 0.82 +1.0 80,349 - Pf1N1B4_5335 0.82 +1.7 80,349 - Pf1N1B4_5335 0.82 -1.1 80,380 + +0.1 80,584 - +3.3 80,628 + -1.0 80,628 + -0.1 80,629 - +1.1 80,629 - -0.5 80,629 - -0.1 80,629 - -0.5 80,695 + +1.7 80,696 - -0.8 80,696 - +1.7 80,696 - +2.7 80,696 - -1.3 80,707 - -0.3 80,707 - -1.3 80,817 + -0.8 80,817 + -0.7 80,857 + -0.8 80,858 - -0.7 81,059 - Pf1N1B4_5336 0.60 -0.3 81,061 + -1.3 81,329 + -0.3 81,329 + -2.7 81,329 + +1.5 81,329 + +0.1 81,330 - -0.7 81,330 - +0.7 81,330 - -1.4 81,330 - +0.7 81,330 - -0.3 81,333 + -1.8 81,333 + +0.2 81,333 + -0.3 81,334 - +0.7 81,334 - +0.7 81,334 - -0.1
Or see this region's nucleotide sequence