Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5253 and Pf1N1B4_5254 are separated by 277 nucleotides
Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045
_5253
Pf1N1B4_5254 - hypothetical protein, at 12,323 to 13,168
_5254
Position (kb) 12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.409 kb on - strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.424 kb on - strand, within Pf1N1B4_5253 at 11.432 kb on + strand, within Pf1N1B4_5253 at 11.432 kb on + strand at 11.433 kb on - strand, within Pf1N1B4_5253 at 11.790 kb on - strand at 11.790 kb on - strand, within Pf1N1B4_5253 at 11.864 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.969 kb on + strand at 11.969 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.068 kb on - strand at 12.068 kb on - strand at 12.153 kb on + strand at 12.418 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.546 kb on + strand, within Pf1N1B4_5254 at 12.547 kb on - strand at 12.547 kb on - strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.741 kb on + strand, within Pf1N1B4_5254 at 12.859 kb on + strand, within Pf1N1B4_5254 at 12.860 kb on - strand at 12.860 kb on - strand, within Pf1N1B4_5254 at 12.952 kb on + strand, within Pf1N1B4_5254 at 12.953 kb on - strand, within Pf1N1B4_5254 at 13.077 kb on + strand, within Pf1N1B4_5254 at 13.086 kb on + strand at 13.086 kb on + strand at 13.175 kb on - strand at 13.175 kb on - strand at 13.185 kb on + strand at 13.185 kb on + strand at 13.185 kb on + strand at 13.185 kb on + strand at 13.186 kb on - strand at 13.186 kb on - strand at 13.200 kb on - strand at 13.212 kb on + strand at 13.213 kb on - strand at 13.224 kb on + strand at 13.297 kb on + strand at 13.382 kb on + strand at 13.410 kb on - strand at 13.525 kb on + strand at 13.754 kb on + strand at 13.822 kb on - strand at 13.822 kb on - strand at 13.822 kb on - strand at 13.964 kb on - strand at 14.101 kb on + strand at 14.148 kb on + strand at 14.148 kb on + strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.162 kb on + strand at 14.162 kb on + strand at 14.163 kb on - strand at 14.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 11,408 + Pf1N1B4_5253 0.16 +1.2 11,408 + Pf1N1B4_5253 0.16 -0.7 11,408 + Pf1N1B4_5253 0.16 +0.1 11,408 + Pf1N1B4_5253 0.16 -0.8 11,409 - Pf1N1B4_5253 0.16 +1.0 11,423 + Pf1N1B4_5253 0.18 +0.4 11,423 + Pf1N1B4_5253 0.18 +0.7 11,424 - Pf1N1B4_5253 0.18 -1.3 11,432 + Pf1N1B4_5253 0.19 -1.4 11,432 + -1.3 11,433 - Pf1N1B4_5253 0.20 -0.8 11,790 - -0.6 11,790 - Pf1N1B4_5253 0.66 +1.2 11,864 + Pf1N1B4_5253 0.76 +0.2 11,920 + Pf1N1B4_5253 0.83 -0.5 11,920 + Pf1N1B4_5253 0.83 +2.3 11,920 + Pf1N1B4_5253 0.83 +0.1 11,920 + Pf1N1B4_5253 0.83 -1.3 11,921 - Pf1N1B4_5253 0.84 +0.4 11,921 - Pf1N1B4_5253 0.84 +0.7 11,924 + Pf1N1B4_5253 0.84 -0.0 11,924 + Pf1N1B4_5253 0.84 +1.7 11,924 + Pf1N1B4_5253 0.84 +1.0 11,925 - Pf1N1B4_5253 0.84 -0.3 11,925 - Pf1N1B4_5253 0.84 -0.2 11,969 + +2.7 11,969 + +0.7 12,033 + +1.0 12,033 + -0.1 12,033 + +0.7 12,033 + -0.3 12,034 - +0.4 12,034 - +0.2 12,034 - -0.1 12,068 - +0.7 12,068 - -0.5 12,153 + -0.6 12,418 - Pf1N1B4_5254 0.11 -0.3 12,485 - Pf1N1B4_5254 0.19 -0.4 12,485 - Pf1N1B4_5254 0.19 -0.8 12,485 - Pf1N1B4_5254 0.19 +0.0 12,546 + Pf1N1B4_5254 0.26 -0.8 12,547 - -0.6 12,547 - Pf1N1B4_5254 0.26 -1.3 12,549 + Pf1N1B4_5254 0.27 -2.6 12,549 + Pf1N1B4_5254 0.27 -0.3 12,549 + Pf1N1B4_5254 0.27 -1.3 12,741 + Pf1N1B4_5254 0.49 +1.5 12,859 + Pf1N1B4_5254 0.63 -1.1 12,860 - +0.3 12,860 - Pf1N1B4_5254 0.63 -1.5 12,952 + Pf1N1B4_5254 0.74 -0.4 12,953 - Pf1N1B4_5254 0.74 -0.5 13,077 + Pf1N1B4_5254 0.89 +0.7 13,086 + +2.0 13,086 + -0.6 13,175 - -0.4 13,175 - -0.3 13,185 + +0.4 13,185 + -0.6 13,185 + -1.3 13,185 + +1.7 13,186 - +0.3 13,186 - +1.2 13,200 - +1.7 13,212 + +0.9 13,213 - -2.8 13,224 + +1.1 13,297 + -0.8 13,382 + -0.1 13,410 - -0.8 13,525 + -1.0 13,754 + -0.3 13,822 - -0.5 13,822 - -1.6 13,822 - +1.5 13,964 - -1.3 14,101 + +1.0 14,148 + -0.3 14,148 + +0.1 14,149 - -1.6 14,149 - -0.3 14,149 - +0.7 14,149 - +0.3 14,149 - -0.2 14,162 + +2.1 14,162 + -0.4 14,163 - -0.5 14,163 - -2.4
Or see this region's nucleotide sequence