Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5085

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5084 and Pf1N1B4_5085 are separated by 139 nucleotidesPf1N1B4_5085 and Pf1N1B4_5086 are separated by 29 nucleotidesPf1N1B4_5086 and Pf1N1B4_5087 are separated by 23 nucleotides Pf1N1B4_5084 - Capsular polysaccharide synthesis enzyme CpsD, exopolysaccharide synthesis, at 5,632,777 to 5,635,023 _5084 Pf1N1B4_5085 - Capsular polysaccharide synthesis enzyme CpsA, sugar transferase, at 5,635,163 to 5,636,554 _5085 Pf1N1B4_5086 - N-acetylmannosaminyltransferase (EC 2.4.1.187), at 5,636,584 to 5,637,291 _5086 Pf1N1B4_5087 - Glycosyl transferase, group 1 family protein, at 5,637,315 to 5,638,394 _5087 Position (kb) 5635 5636 5637Strain fitness (log2 ratio) -3 -2 -1 0 1at 5634.447 kb on - strand, within Pf1N1B4_5084at 5634.844 kb on + strandat 5634.845 kb on - strandat 5634.882 kb on - strandat 5635.010 kb on - strandat 5635.022 kb on - strandat 5635.065 kb on - strandat 5635.065 kb on - strandat 5635.092 kb on + strandat 5635.092 kb on + strandat 5635.346 kb on + strand, within Pf1N1B4_5085at 5635.551 kb on - strand, within Pf1N1B4_5085at 5635.627 kb on + strand, within Pf1N1B4_5085at 5635.627 kb on + strand, within Pf1N1B4_5085at 5635.628 kb on - strand, within Pf1N1B4_5085at 5635.773 kb on + strand, within Pf1N1B4_5085at 5635.773 kb on + strand, within Pf1N1B4_5085at 5635.785 kb on - strand, within Pf1N1B4_5085at 5635.785 kb on - strand, within Pf1N1B4_5085at 5635.830 kb on - strand, within Pf1N1B4_5085at 5635.937 kb on + strand, within Pf1N1B4_5085at 5636.023 kb on + strand, within Pf1N1B4_5085at 5636.253 kb on + strand, within Pf1N1B4_5085at 5636.254 kb on - strand, within Pf1N1B4_5085at 5636.355 kb on - strand, within Pf1N1B4_5085at 5636.471 kb on + strandat 5636.549 kb on + strandat 5636.549 kb on + strandat 5636.550 kb on - strandat 5636.612 kb on - strandat 5637.127 kb on + strand, within Pf1N1B4_5086at 5637.420 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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5,634,447 - Pf1N1B4_5084 0.74 +0.3
5,634,844 + -0.7
5,634,845 - +0.3
5,634,882 - +0.3
5,635,010 - -2.0
5,635,022 - -2.0
5,635,065 - +0.3
5,635,065 - -1.3
5,635,092 + +0.3
5,635,092 + +1.5
5,635,346 + Pf1N1B4_5085 0.13 -1.7
5,635,551 - Pf1N1B4_5085 0.28 -0.8
5,635,627 + Pf1N1B4_5085 0.33 +0.3
5,635,627 + Pf1N1B4_5085 0.33 +0.7
5,635,628 - Pf1N1B4_5085 0.33 -0.8
5,635,773 + Pf1N1B4_5085 0.44 -0.9
5,635,773 + Pf1N1B4_5085 0.44 -0.2
5,635,785 - Pf1N1B4_5085 0.45 +0.1
5,635,785 - Pf1N1B4_5085 0.45 -0.6
5,635,830 - Pf1N1B4_5085 0.48 -0.1
5,635,937 + Pf1N1B4_5085 0.56 -0.3
5,636,023 + Pf1N1B4_5085 0.62 -0.5
5,636,253 + Pf1N1B4_5085 0.78 -1.6
5,636,254 - Pf1N1B4_5085 0.78 -2.8
5,636,355 - Pf1N1B4_5085 0.86 -0.9
5,636,471 + +0.3
5,636,549 + -0.7
5,636,549 + -0.7
5,636,550 - +0.3
5,636,612 - +1.0
5,637,127 + Pf1N1B4_5086 0.77 -1.7
5,637,420 + -0.5

Or see this region's nucleotide sequence