Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5084 and Pf1N1B4_5085 are separated by 139 nucleotides Pf1N1B4_5085 and Pf1N1B4_5086 are separated by 29 nucleotides Pf1N1B4_5086 and Pf1N1B4_5087 are separated by 23 nucleotides
Pf1N1B4_5084 - Capsular polysaccharide synthesis enzyme CpsD, exopolysaccharide synthesis, at 5,632,777 to 5,635,023
_5084
Pf1N1B4_5085 - Capsular polysaccharide synthesis enzyme CpsA, sugar transferase, at 5,635,163 to 5,636,554
_5085
Pf1N1B4_5086 - N-acetylmannosaminyltransferase (EC 2.4.1.187), at 5,636,584 to 5,637,291
_5086
Pf1N1B4_5087 - Glycosyl transferase, group 1 family protein, at 5,637,315 to 5,638,394
_5087
Position (kb)
5635
5636
5637 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5634.447 kb on - strand, within Pf1N1B4_5084 at 5634.844 kb on + strand at 5634.845 kb on - strand at 5634.882 kb on - strand at 5635.010 kb on - strand at 5635.022 kb on - strand at 5635.065 kb on - strand at 5635.065 kb on - strand at 5635.092 kb on + strand at 5635.092 kb on + strand at 5635.346 kb on + strand, within Pf1N1B4_5085 at 5635.551 kb on - strand, within Pf1N1B4_5085 at 5635.627 kb on + strand, within Pf1N1B4_5085 at 5635.627 kb on + strand, within Pf1N1B4_5085 at 5635.628 kb on - strand, within Pf1N1B4_5085 at 5635.773 kb on + strand, within Pf1N1B4_5085 at 5635.773 kb on + strand, within Pf1N1B4_5085 at 5635.785 kb on - strand, within Pf1N1B4_5085 at 5635.785 kb on - strand, within Pf1N1B4_5085 at 5635.830 kb on - strand, within Pf1N1B4_5085 at 5635.937 kb on + strand, within Pf1N1B4_5085 at 5636.023 kb on + strand, within Pf1N1B4_5085 at 5636.253 kb on + strand, within Pf1N1B4_5085 at 5636.254 kb on - strand, within Pf1N1B4_5085 at 5636.355 kb on - strand, within Pf1N1B4_5085 at 5636.471 kb on + strand at 5636.549 kb on + strand at 5636.549 kb on + strand at 5636.550 kb on - strand at 5636.612 kb on - strand at 5637.127 kb on + strand, within Pf1N1B4_5086 at 5637.420 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 5,634,447 - Pf1N1B4_5084 0.74 +0.3 5,634,844 + -0.7 5,634,845 - +0.3 5,634,882 - +0.3 5,635,010 - -2.0 5,635,022 - -2.0 5,635,065 - +0.3 5,635,065 - -1.3 5,635,092 + +0.3 5,635,092 + +1.5 5,635,346 + Pf1N1B4_5085 0.13 -1.7 5,635,551 - Pf1N1B4_5085 0.28 -0.8 5,635,627 + Pf1N1B4_5085 0.33 +0.3 5,635,627 + Pf1N1B4_5085 0.33 +0.7 5,635,628 - Pf1N1B4_5085 0.33 -0.8 5,635,773 + Pf1N1B4_5085 0.44 -0.9 5,635,773 + Pf1N1B4_5085 0.44 -0.2 5,635,785 - Pf1N1B4_5085 0.45 +0.1 5,635,785 - Pf1N1B4_5085 0.45 -0.6 5,635,830 - Pf1N1B4_5085 0.48 -0.1 5,635,937 + Pf1N1B4_5085 0.56 -0.3 5,636,023 + Pf1N1B4_5085 0.62 -0.5 5,636,253 + Pf1N1B4_5085 0.78 -1.6 5,636,254 - Pf1N1B4_5085 0.78 -2.8 5,636,355 - Pf1N1B4_5085 0.86 -0.9 5,636,471 + +0.3 5,636,549 + -0.7 5,636,549 + -0.7 5,636,550 - +0.3 5,636,612 - +1.0 5,637,127 + Pf1N1B4_5086 0.77 -1.7 5,637,420 + -0.5
Or see this region's nucleotide sequence