Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_490 and Pf1N1B4_491 are separated by 740 nucleotides Pf1N1B4_491 and Pf1N1B4_492 overlap by 4 nucleotides Pf1N1B4_492 and Pf1N1B4_493 are separated by 18 nucleotides
Pf1N1B4_490 - hypothetical protein, at 582,334 to 582,507
_490
Pf1N1B4_491 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 583,248 to 584,471
_491
Pf1N1B4_492 - Flp pilus assembly protein TadD, contains TPR repeats, at 584,468 to 585,295
_492
Pf1N1B4_493 - hypothetical protein, at 585,314 to 585,583
_493
Position (kb)
583
584
585 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 582.271 kb on + strand at 582.271 kb on + strand at 582.272 kb on - strand at 582.319 kb on - strand at 582.319 kb on - strand at 582.440 kb on + strand, within Pf1N1B4_490 at 582.440 kb on + strand, within Pf1N1B4_490 at 582.440 kb on + strand, within Pf1N1B4_490 at 582.441 kb on - strand, within Pf1N1B4_490 at 582.441 kb on - strand, within Pf1N1B4_490 at 582.466 kb on + strand, within Pf1N1B4_490 at 582.466 kb on + strand, within Pf1N1B4_490 at 582.480 kb on + strand, within Pf1N1B4_490 at 582.480 kb on + strand, within Pf1N1B4_490 at 582.505 kb on + strand at 582.505 kb on + strand at 582.505 kb on + strand at 582.506 kb on - strand at 582.506 kb on - strand at 582.506 kb on - strand at 582.511 kb on + strand at 582.512 kb on - strand at 582.512 kb on - strand at 582.525 kb on + strand at 582.525 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.538 kb on - strand at 582.538 kb on - strand at 582.538 kb on - strand at 582.564 kb on + strand at 582.565 kb on - strand at 582.588 kb on + strand at 582.588 kb on + strand at 582.640 kb on + strand at 582.640 kb on + strand at 582.641 kb on - strand at 582.641 kb on - strand at 582.641 kb on - strand at 582.691 kb on - strand at 582.811 kb on + strand at 582.812 kb on - strand at 582.812 kb on - strand at 582.835 kb on + strand at 582.894 kb on + strand at 582.895 kb on - strand at 582.895 kb on - strand at 582.895 kb on - strand at 582.902 kb on + strand at 582.903 kb on - strand at 582.989 kb on - strand at 583.011 kb on + strand at 583.011 kb on + strand at 583.011 kb on + strand at 583.011 kb on + strand at 583.012 kb on - strand at 583.012 kb on - strand at 583.013 kb on + strand at 583.049 kb on + strand at 583.061 kb on + strand at 583.061 kb on + strand at 583.061 kb on + strand at 583.062 kb on - strand at 583.129 kb on - strand at 583.187 kb on + strand at 583.187 kb on + strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.188 kb on - strand at 583.246 kb on + strand at 583.247 kb on - strand at 583.489 kb on + strand, within Pf1N1B4_491 at 583.489 kb on + strand, within Pf1N1B4_491 at 583.490 kb on - strand, within Pf1N1B4_491 at 583.490 kb on - strand, within Pf1N1B4_491 at 583.490 kb on - strand, within Pf1N1B4_491 at 583.670 kb on - strand, within Pf1N1B4_491 at 583.670 kb on - strand, within Pf1N1B4_491 at 583.670 kb on - strand, within Pf1N1B4_491 at 583.879 kb on - strand, within Pf1N1B4_491 at 583.995 kb on + strand, within Pf1N1B4_491 at 583.996 kb on - strand, within Pf1N1B4_491 at 584.140 kb on + strand, within Pf1N1B4_491 at 584.140 kb on + strand, within Pf1N1B4_491 at 584.365 kb on + strand at 584.365 kb on + strand at 584.365 kb on + strand at 584.366 kb on - strand at 584.366 kb on - strand at 584.366 kb on - strand at 584.397 kb on + strand at 584.398 kb on - strand at 584.398 kb on - strand at 584.569 kb on - strand, within Pf1N1B4_492 at 584.663 kb on - strand, within Pf1N1B4_492 at 584.738 kb on + strand, within Pf1N1B4_492 at 584.739 kb on - strand, within Pf1N1B4_492 at 584.846 kb on + strand, within Pf1N1B4_492 at 584.846 kb on + strand, within Pf1N1B4_492 at 584.847 kb on - strand, within Pf1N1B4_492 at 585.008 kb on + strand, within Pf1N1B4_492 at 585.009 kb on - strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.025 kb on + strand, within Pf1N1B4_492 at 585.026 kb on - strand, within Pf1N1B4_492 at 585.098 kb on - strand, within Pf1N1B4_492 at 585.116 kb on + strand, within Pf1N1B4_492 at 585.117 kb on - strand at 585.117 kb on - strand, within Pf1N1B4_492 at 585.254 kb on - strand at 585.254 kb on - strand at 585.293 kb on + strand at 585.294 kb on - strand at 585.294 kb on - strand at 585.294 kb on - strand at 585.301 kb on - strand at 585.319 kb on + strand at 585.429 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand at 585.444 kb on + strand, within Pf1N1B4_493 at 585.444 kb on + strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493 at 585.445 kb on - strand, within Pf1N1B4_493
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 582,271 + -0.7 582,271 + -1.3 582,272 - -0.6 582,319 - +0.9 582,319 - -1.6 582,440 + Pf1N1B4_490 0.61 +0.3 582,440 + Pf1N1B4_490 0.61 -1.6 582,440 + Pf1N1B4_490 0.61 -1.3 582,441 - Pf1N1B4_490 0.61 +0.7 582,441 - Pf1N1B4_490 0.61 -0.5 582,466 + Pf1N1B4_490 0.76 +0.5 582,466 + Pf1N1B4_490 0.76 -0.9 582,480 + Pf1N1B4_490 0.84 -0.3 582,480 + Pf1N1B4_490 0.84 -1.3 582,505 + +0.4 582,505 + +0.7 582,505 + +0.2 582,506 - +0.7 582,506 - +0.1 582,506 - -0.3 582,511 + -0.9 582,512 - +0.8 582,512 - -0.7 582,525 + -0.3 582,525 + -0.1 582,537 + +0.7 582,537 + +0.5 582,537 + -0.4 582,537 + +2.0 582,538 - +1.5 582,538 - -1.5 582,538 - +1.3 582,564 + -0.6 582,565 - -1.1 582,588 + -0.5 582,588 + -0.9 582,640 + -0.5 582,640 + -1.1 582,641 - +0.1 582,641 - -0.6 582,641 - -0.9 582,691 - -1.7 582,811 + -1.4 582,812 - +1.9 582,812 - -1.0 582,835 + -0.6 582,894 + +0.7 582,895 - +2.3 582,895 - -0.3 582,895 - -0.9 582,902 + -1.5 582,903 - -0.9 582,989 - -0.5 583,011 + +2.0 583,011 + +0.7 583,011 + +1.7 583,011 + -1.3 583,012 - -1.1 583,012 - +0.2 583,013 + +0.4 583,049 + +0.1 583,061 + -1.5 583,061 + -1.1 583,061 + -0.3 583,062 - +0.1 583,129 - -1.3 583,187 + -0.5 583,187 + +1.0 583,188 - -2.5 583,188 - -2.3 583,188 - -0.1 583,188 - +1.2 583,188 - -0.6 583,188 - +0.1 583,246 + +0.7 583,247 - -0.0 583,489 + Pf1N1B4_491 0.20 -0.4 583,489 + Pf1N1B4_491 0.20 -0.6 583,490 - Pf1N1B4_491 0.20 -1.2 583,490 - Pf1N1B4_491 0.20 -1.5 583,490 - Pf1N1B4_491 0.20 -0.6 583,670 - Pf1N1B4_491 0.34 -0.3 583,670 - Pf1N1B4_491 0.34 -0.9 583,670 - Pf1N1B4_491 0.34 +0.5 583,879 - Pf1N1B4_491 0.52 -1.7 583,995 + Pf1N1B4_491 0.61 +0.7 583,996 - Pf1N1B4_491 0.61 -0.9 584,140 + Pf1N1B4_491 0.73 -0.3 584,140 + Pf1N1B4_491 0.73 +0.3 584,365 + -0.9 584,365 + -1.6 584,365 + -2.7 584,366 - -0.1 584,366 - -1.7 584,366 - -0.1 584,397 + -0.9 584,398 - +0.7 584,398 - -0.3 584,569 - Pf1N1B4_492 0.12 +0.2 584,663 - Pf1N1B4_492 0.24 -0.2 584,738 + Pf1N1B4_492 0.33 -0.4 584,739 - Pf1N1B4_492 0.33 +0.6 584,846 + Pf1N1B4_492 0.46 +1.0 584,846 + Pf1N1B4_492 0.46 -0.9 584,847 - Pf1N1B4_492 0.46 -1.6 585,008 + Pf1N1B4_492 0.65 -0.9 585,009 - Pf1N1B4_492 0.65 -0.3 585,025 + Pf1N1B4_492 0.67 -0.5 585,025 + Pf1N1B4_492 0.67 -0.3 585,025 + Pf1N1B4_492 0.67 -0.4 585,026 - Pf1N1B4_492 0.67 +0.7 585,098 - Pf1N1B4_492 0.76 +2.1 585,116 + Pf1N1B4_492 0.78 +0.4 585,117 - -0.3 585,117 - Pf1N1B4_492 0.78 +0.5 585,254 - +1.3 585,254 - -0.5 585,293 + -0.3 585,294 - -0.7 585,294 - -0.4 585,294 - +1.1 585,301 - -0.9 585,319 + -1.6 585,429 + Pf1N1B4_493 0.43 +0.6 585,444 + Pf1N1B4_493 0.48 -0.0 585,444 + Pf1N1B4_493 0.48 -0.3 585,444 + Pf1N1B4_493 0.48 -1.2 585,444 + +0.7 585,444 + Pf1N1B4_493 0.48 -0.3 585,444 + Pf1N1B4_493 0.48 +0.7 585,445 - Pf1N1B4_493 0.49 +0.7 585,445 - Pf1N1B4_493 0.49 +0.9 585,445 - Pf1N1B4_493 0.49 +0.2
Or see this region's nucleotide sequence