Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4892 and Pf1N1B4_4893 are separated by 51 nucleotides Pf1N1B4_4893 and Pf1N1B4_4894 are separated by 31 nucleotides Pf1N1B4_4894 and Pf1N1B4_4895 are separated by 44 nucleotides
Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121
_4892
Pf1N1B4_4893 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 5,415,173 to 5,416,579
_4893
Pf1N1B4_4894 - Nucleoside-diphosphate-sugar epimerases, at 5,416,611 to 5,416,952
_4894
Pf1N1B4_4895 - Putrescine ABC transporter, periplasmic putrescine-binding protein, at 5,416,997 to 5,418,082
_4895
Position (kb)
5415
5416
5417 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5414.306 kb on - strand at 5414.432 kb on + strand at 5414.432 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.540 kb on - strand, within Pf1N1B4_4892 at 5414.810 kb on + strand, within Pf1N1B4_4892 at 5414.880 kb on - strand, within Pf1N1B4_4892 at 5414.913 kb on - strand, within Pf1N1B4_4892 at 5414.919 kb on - strand, within Pf1N1B4_4892 at 5415.033 kb on - strand, within Pf1N1B4_4892 at 5415.308 kb on + strand at 5415.308 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.710 kb on - strand, within Pf1N1B4_4893 at 5415.713 kb on + strand at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.797 kb on + strand at 5416.040 kb on + strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.071 kb on + strand, within Pf1N1B4_4893 at 5416.143 kb on + strand, within Pf1N1B4_4893 at 5416.144 kb on - strand, within Pf1N1B4_4893 at 5416.228 kb on + strand, within Pf1N1B4_4893 at 5416.228 kb on + strand, within Pf1N1B4_4893 at 5416.604 kb on + strand at 5416.605 kb on - strand at 5416.854 kb on + strand, within Pf1N1B4_4894 at 5416.854 kb on + strand at 5416.855 kb on - strand at 5416.855 kb on - strand at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.859 kb on - strand, within Pf1N1B4_4894 at 5416.936 kb on - strand at 5416.936 kb on - strand at 5417.148 kb on - strand, within Pf1N1B4_4895 at 5417.369 kb on + strand, within Pf1N1B4_4895 at 5417.369 kb on + strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 5,414,306 - +0.3 5,414,432 + +0.9 5,414,432 + Pf1N1B4_4892 0.15 -0.3 5,414,483 + Pf1N1B4_4892 0.22 +1.9 5,414,483 + Pf1N1B4_4892 0.22 +0.8 5,414,540 - Pf1N1B4_4892 0.29 +0.3 5,414,810 + Pf1N1B4_4892 0.62 -0.9 5,414,880 - Pf1N1B4_4892 0.70 -0.5 5,414,913 - Pf1N1B4_4892 0.74 -0.1 5,414,919 - Pf1N1B4_4892 0.75 -0.5 5,415,033 - Pf1N1B4_4892 0.89 +0.3 5,415,308 + -1.0 5,415,308 + -0.2 5,415,311 + -1.3 5,415,311 + -1.2 5,415,311 + +0.3 5,415,710 - Pf1N1B4_4893 0.38 +0.6 5,415,713 + -1.3 5,415,713 + Pf1N1B4_4893 0.38 -0.5 5,415,713 + Pf1N1B4_4893 0.38 +0.3 5,415,713 + Pf1N1B4_4893 0.38 -0.9 5,415,714 - Pf1N1B4_4893 0.38 +1.4 5,415,714 - Pf1N1B4_4893 0.38 +1.5 5,415,797 + +1.9 5,416,040 + Pf1N1B4_4893 0.62 +0.3 5,416,041 - -0.7 5,416,041 - Pf1N1B4_4893 0.62 +2.6 5,416,041 - Pf1N1B4_4893 0.62 +0.9 5,416,041 - +0.9 5,416,071 + Pf1N1B4_4893 0.64 +0.3 5,416,143 + Pf1N1B4_4893 0.69 +2.6 5,416,144 - Pf1N1B4_4893 0.69 +0.1 5,416,228 + Pf1N1B4_4893 0.75 -0.5 5,416,228 + Pf1N1B4_4893 0.75 +1.6 5,416,604 + -2.3 5,416,605 - -1.3 5,416,854 + Pf1N1B4_4894 0.71 -0.7 5,416,854 + +1.3 5,416,855 - -0.7 5,416,855 - -0.7 5,416,859 - Pf1N1B4_4894 0.73 -1.7 5,416,859 - Pf1N1B4_4894 0.73 +0.1 5,416,859 - Pf1N1B4_4894 0.73 -0.5 5,416,859 - Pf1N1B4_4894 0.73 +0.3 5,416,859 - Pf1N1B4_4894 0.73 +2.3 5,416,859 - Pf1N1B4_4894 0.73 +0.4 5,416,859 - Pf1N1B4_4894 0.73 +0.6 5,416,859 - Pf1N1B4_4894 0.73 +0.7 5,416,859 - Pf1N1B4_4894 0.73 -0.3 5,416,936 - +0.1 5,416,936 - -0.3 5,417,148 - Pf1N1B4_4895 0.14 -0.7 5,417,369 + Pf1N1B4_4895 0.34 +0.9 5,417,369 + Pf1N1B4_4895 0.34 -0.9 5,417,550 - Pf1N1B4_4895 0.51 -0.7 5,417,550 - Pf1N1B4_4895 0.51 -0.5 5,417,550 - Pf1N1B4_4895 0.51 -0.7
Or see this region's nucleotide sequence