Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4893

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4892 and Pf1N1B4_4893 are separated by 51 nucleotidesPf1N1B4_4893 and Pf1N1B4_4894 are separated by 31 nucleotidesPf1N1B4_4894 and Pf1N1B4_4895 are separated by 44 nucleotides Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121 _4892 Pf1N1B4_4893 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 5,415,173 to 5,416,579 _4893 Pf1N1B4_4894 - Nucleoside-diphosphate-sugar epimerases, at 5,416,611 to 5,416,952 _4894 Pf1N1B4_4895 - Putrescine ABC transporter, periplasmic putrescine-binding protein, at 5,416,997 to 5,418,082 _4895 Position (kb) 5415 5416 5417Strain fitness (log2 ratio) -2 -1 0 1 2at 5414.306 kb on - strandat 5414.432 kb on + strandat 5414.432 kb on + strand, within Pf1N1B4_4892at 5414.483 kb on + strand, within Pf1N1B4_4892at 5414.483 kb on + strand, within Pf1N1B4_4892at 5414.540 kb on - strand, within Pf1N1B4_4892at 5414.810 kb on + strand, within Pf1N1B4_4892at 5414.880 kb on - strand, within Pf1N1B4_4892at 5414.913 kb on - strand, within Pf1N1B4_4892at 5414.919 kb on - strand, within Pf1N1B4_4892at 5415.033 kb on - strand, within Pf1N1B4_4892at 5415.308 kb on + strandat 5415.308 kb on + strandat 5415.311 kb on + strandat 5415.311 kb on + strandat 5415.311 kb on + strandat 5415.710 kb on - strand, within Pf1N1B4_4893at 5415.713 kb on + strandat 5415.713 kb on + strand, within Pf1N1B4_4893at 5415.713 kb on + strand, within Pf1N1B4_4893at 5415.713 kb on + strand, within Pf1N1B4_4893at 5415.714 kb on - strand, within Pf1N1B4_4893at 5415.714 kb on - strand, within Pf1N1B4_4893at 5415.797 kb on + strandat 5416.040 kb on + strand, within Pf1N1B4_4893at 5416.041 kb on - strandat 5416.041 kb on - strand, within Pf1N1B4_4893at 5416.041 kb on - strand, within Pf1N1B4_4893at 5416.041 kb on - strandat 5416.071 kb on + strand, within Pf1N1B4_4893at 5416.143 kb on + strand, within Pf1N1B4_4893at 5416.144 kb on - strand, within Pf1N1B4_4893at 5416.228 kb on + strand, within Pf1N1B4_4893at 5416.228 kb on + strand, within Pf1N1B4_4893at 5416.604 kb on + strandat 5416.605 kb on - strandat 5416.854 kb on + strand, within Pf1N1B4_4894at 5416.854 kb on + strandat 5416.855 kb on - strandat 5416.855 kb on - strandat 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.859 kb on - strand, within Pf1N1B4_4894at 5416.936 kb on - strandat 5416.936 kb on - strandat 5417.148 kb on - strand, within Pf1N1B4_4895at 5417.369 kb on + strand, within Pf1N1B4_4895at 5417.369 kb on + strand, within Pf1N1B4_4895at 5417.550 kb on - strand, within Pf1N1B4_4895at 5417.550 kb on - strand, within Pf1N1B4_4895at 5417.550 kb on - strand, within Pf1N1B4_4895

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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5,414,306 - +0.3
5,414,432 + +0.9
5,414,432 + Pf1N1B4_4892 0.15 -0.3
5,414,483 + Pf1N1B4_4892 0.22 +1.9
5,414,483 + Pf1N1B4_4892 0.22 +0.8
5,414,540 - Pf1N1B4_4892 0.29 +0.3
5,414,810 + Pf1N1B4_4892 0.62 -0.9
5,414,880 - Pf1N1B4_4892 0.70 -0.5
5,414,913 - Pf1N1B4_4892 0.74 -0.1
5,414,919 - Pf1N1B4_4892 0.75 -0.5
5,415,033 - Pf1N1B4_4892 0.89 +0.3
5,415,308 + -1.0
5,415,308 + -0.2
5,415,311 + -1.3
5,415,311 + -1.2
5,415,311 + +0.3
5,415,710 - Pf1N1B4_4893 0.38 +0.6
5,415,713 + -1.3
5,415,713 + Pf1N1B4_4893 0.38 -0.5
5,415,713 + Pf1N1B4_4893 0.38 +0.3
5,415,713 + Pf1N1B4_4893 0.38 -0.9
5,415,714 - Pf1N1B4_4893 0.38 +1.4
5,415,714 - Pf1N1B4_4893 0.38 +1.5
5,415,797 + +1.9
5,416,040 + Pf1N1B4_4893 0.62 +0.3
5,416,041 - -0.7
5,416,041 - Pf1N1B4_4893 0.62 +2.6
5,416,041 - Pf1N1B4_4893 0.62 +0.9
5,416,041 - +0.9
5,416,071 + Pf1N1B4_4893 0.64 +0.3
5,416,143 + Pf1N1B4_4893 0.69 +2.6
5,416,144 - Pf1N1B4_4893 0.69 +0.1
5,416,228 + Pf1N1B4_4893 0.75 -0.5
5,416,228 + Pf1N1B4_4893 0.75 +1.6
5,416,604 + -2.3
5,416,605 - -1.3
5,416,854 + Pf1N1B4_4894 0.71 -0.7
5,416,854 + +1.3
5,416,855 - -0.7
5,416,855 - -0.7
5,416,859 - Pf1N1B4_4894 0.73 -1.7
5,416,859 - Pf1N1B4_4894 0.73 +0.1
5,416,859 - Pf1N1B4_4894 0.73 -0.5
5,416,859 - Pf1N1B4_4894 0.73 +0.3
5,416,859 - Pf1N1B4_4894 0.73 +2.3
5,416,859 - Pf1N1B4_4894 0.73 +0.4
5,416,859 - Pf1N1B4_4894 0.73 +0.6
5,416,859 - Pf1N1B4_4894 0.73 +0.7
5,416,859 - Pf1N1B4_4894 0.73 -0.3
5,416,936 - +0.1
5,416,936 - -0.3
5,417,148 - Pf1N1B4_4895 0.14 -0.7
5,417,369 + Pf1N1B4_4895 0.34 +0.9
5,417,369 + Pf1N1B4_4895 0.34 -0.9
5,417,550 - Pf1N1B4_4895 0.51 -0.7
5,417,550 - Pf1N1B4_4895 0.51 -0.5
5,417,550 - Pf1N1B4_4895 0.51 -0.7

Or see this region's nucleotide sequence