Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4865

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4864 and Pf1N1B4_4865 are separated by 12 nucleotidesPf1N1B4_4865 and Pf1N1B4_4866 are separated by 307 nucleotidesPf1N1B4_4866 and Pf1N1B4_4867 are separated by 40 nucleotides Pf1N1B4_4864 - Multidrug efflux transporter MexF, at 5,379,763 to 5,382,954 _4864 Pf1N1B4_4865 - Multidrug efflux membrane fusion protein MexE, at 5,382,967 to 5,384,220 _4865 Pf1N1B4_4866 - hypothetical protein, at 5,384,528 to 5,384,653 _4866 Pf1N1B4_4867 - FIG00464907: hypothetical protein, at 5,384,694 to 5,385,713 _4867 Position (kb) 5382 5383 5384 5385Strain fitness (log2 ratio) -1 0 1 2at 5382.196 kb on - strand, within Pf1N1B4_4864at 5382.479 kb on + strand, within Pf1N1B4_4864at 5382.480 kb on - strandat 5382.480 kb on - strand, within Pf1N1B4_4864at 5382.491 kb on + strand, within Pf1N1B4_4864at 5382.492 kb on - strand, within Pf1N1B4_4864at 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.719 kb on + strandat 5382.720 kb on - strandat 5382.720 kb on - strandat 5382.957 kb on + strandat 5382.957 kb on + strandat 5383.331 kb on + strand, within Pf1N1B4_4865at 5383.554 kb on - strand, within Pf1N1B4_4865at 5383.557 kb on + strand, within Pf1N1B4_4865at 5383.558 kb on - strand, within Pf1N1B4_4865at 5383.558 kb on - strand, within Pf1N1B4_4865at 5383.689 kb on + strand, within Pf1N1B4_4865at 5384.239 kb on - strandat 5384.272 kb on + strandat 5384.272 kb on + strandat 5384.273 kb on - strandat 5384.273 kb on - strandat 5384.318 kb on + strandat 5384.320 kb on + strandat 5384.397 kb on + strandat 5384.529 kb on + strandat 5384.530 kb on - strandat 5384.530 kb on - strandat 5384.561 kb on + strand, within Pf1N1B4_4866at 5384.561 kb on + strand, within Pf1N1B4_4866at 5384.599 kb on + strand, within Pf1N1B4_4866at 5384.615 kb on - strand, within Pf1N1B4_4866at 5384.633 kb on + strand, within Pf1N1B4_4866at 5384.633 kb on + strand, within Pf1N1B4_4866at 5385.195 kb on + strand, within Pf1N1B4_4867

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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5,382,196 - Pf1N1B4_4864 0.76 +1.0
5,382,479 + Pf1N1B4_4864 0.85 -0.2
5,382,480 - +0.3
5,382,480 - Pf1N1B4_4864 0.85 -0.1
5,382,491 + Pf1N1B4_4864 0.85 +0.3
5,382,492 - Pf1N1B4_4864 0.85 +1.0
5,382,719 + +0.3
5,382,719 + +0.4
5,382,719 + +2.6
5,382,719 + +0.3
5,382,719 + +0.0
5,382,720 - +1.1
5,382,720 - +1.3
5,382,957 + +1.9
5,382,957 + +0.6
5,383,331 + Pf1N1B4_4865 0.29 -0.1
5,383,554 - Pf1N1B4_4865 0.47 +0.1
5,383,557 + Pf1N1B4_4865 0.47 +0.9
5,383,558 - Pf1N1B4_4865 0.47 +0.5
5,383,558 - Pf1N1B4_4865 0.47 +0.4
5,383,689 + Pf1N1B4_4865 0.58 +0.6
5,384,239 - -0.1
5,384,272 + +1.6
5,384,272 + +1.3
5,384,273 - +2.2
5,384,273 - +1.0
5,384,318 + +1.9
5,384,320 + -0.2
5,384,397 + +0.5
5,384,529 + +1.0
5,384,530 - -0.7
5,384,530 - +0.0
5,384,561 + Pf1N1B4_4866 0.26 +0.3
5,384,561 + Pf1N1B4_4866 0.26 +0.5
5,384,599 + Pf1N1B4_4866 0.56 +1.3
5,384,615 - Pf1N1B4_4866 0.69 -0.2
5,384,633 + Pf1N1B4_4866 0.83 +0.6
5,384,633 + Pf1N1B4_4866 0.83 +0.0
5,385,195 + Pf1N1B4_4867 0.49 -1.7

Or see this region's nucleotide sequence