Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4548 and Pf1N1B4_4549 are separated by 58 nucleotides Pf1N1B4_4549 and Pf1N1B4_4550 are separated by 1 nucleotides
Pf1N1B4_4548 - Acyl carrier protein, putative, at 5,016,496 to 5,017,068
_4548
Pf1N1B4_4549 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 5,017,127 to 5,018,347
_4549
Pf1N1B4_4550 - beta-ketoacyl synthase, putative, at 5,018,349 to 5,019,431
_4550
Position (kb)
5017
5018
5019 Strain fitness (log2 ratio)
-1
0
1
2 at 5016.283 kb on - strand at 5016.407 kb on + strand at 5016.408 kb on - strand at 5016.457 kb on + strand at 5016.460 kb on + strand at 5016.461 kb on - strand at 5016.461 kb on - strand at 5016.464 kb on + strand at 5016.465 kb on - strand at 5016.465 kb on - strand at 5016.494 kb on - strand at 5016.528 kb on + strand at 5016.594 kb on + strand, within Pf1N1B4_4548 at 5016.594 kb on + strand, within Pf1N1B4_4548 at 5016.667 kb on + strand, within Pf1N1B4_4548 at 5016.667 kb on + strand, within Pf1N1B4_4548 at 5017.097 kb on + strand at 5017.098 kb on - strand at 5017.098 kb on - strand at 5017.236 kb on - strand at 5017.403 kb on - strand, within Pf1N1B4_4549 at 5017.703 kb on + strand at 5018.073 kb on + strand, within Pf1N1B4_4549 at 5018.074 kb on - strand, within Pf1N1B4_4549 at 5018.213 kb on + strand at 5018.214 kb on - strand at 5018.355 kb on - strand at 5018.363 kb on + strand at 5018.572 kb on - strand, within Pf1N1B4_4550 at 5018.657 kb on - strand, within Pf1N1B4_4550 at 5018.716 kb on + strand, within Pf1N1B4_4550 at 5018.738 kb on + strand, within Pf1N1B4_4550 at 5018.739 kb on - strand, within Pf1N1B4_4550 at 5018.930 kb on + strand, within Pf1N1B4_4550 at 5018.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 5,016,283 - -0.3 5,016,407 + -0.4 5,016,408 - -0.3 5,016,457 + -0.4 5,016,460 + +1.6 5,016,461 - +0.2 5,016,461 - -0.3 5,016,464 + +0.6 5,016,465 - -1.0 5,016,465 - -0.3 5,016,494 - +1.0 5,016,528 + +0.9 5,016,594 + Pf1N1B4_4548 0.17 +2.3 5,016,594 + Pf1N1B4_4548 0.17 +0.3 5,016,667 + Pf1N1B4_4548 0.30 -0.3 5,016,667 + Pf1N1B4_4548 0.30 +0.3 5,017,097 + +1.2 5,017,098 - +0.1 5,017,098 - +0.3 5,017,236 - -1.1 5,017,403 - Pf1N1B4_4549 0.23 -1.4 5,017,703 + -0.7 5,018,073 + Pf1N1B4_4549 0.77 -1.0 5,018,074 - Pf1N1B4_4549 0.78 +0.5 5,018,213 + -0.3 5,018,214 - +0.3 5,018,355 - -0.3 5,018,363 + -0.1 5,018,572 - Pf1N1B4_4550 0.21 -0.7 5,018,657 - Pf1N1B4_4550 0.28 +1.9 5,018,716 + Pf1N1B4_4550 0.34 +0.9 5,018,738 + Pf1N1B4_4550 0.36 +0.7 5,018,739 - Pf1N1B4_4550 0.36 -1.5 5,018,930 + Pf1N1B4_4550 0.54 -0.3 5,018,950 - -0.7
Or see this region's nucleotide sequence