Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4507 and Pf1N1B4_4508 overlap by 16 nucleotides Pf1N1B4_4508 and Pf1N1B4_4509 overlap by 4 nucleotides Pf1N1B4_4509 and Pf1N1B4_4510 are separated by 16 nucleotides Pf1N1B4_4510 and Pf1N1B4_4511 are separated by 45 nucleotides
Pf1N1B4_4507 - Sensory box histidine kinase/response regulator, at 4,969,873 to 4,971,558
_4507
Pf1N1B4_4508 - hypothetical protein, at 4,971,543 to 4,971,659
_4508
Pf1N1B4_4509 - Aldose 1-epimerase, at 4,971,656 to 4,972,417
_4509
Pf1N1B4_4510 - Gluconolactonase (EC 3.1.1.17), at 4,972,434 to 4,973,327
_4510
Pf1N1B4_4511 - D-galacturonate dehydrogenase (EC 1.1.1.203) <small>(from data)</small>, at 4,973,373 to 4,974,185
_4511
Position (kb) 4971
4972
4973 Strain fitness (log2 ratio) -1
0
1
2 at 4971.705 kb on + strand at 4971.708 kb on + strand at 4971.708 kb on + strand at 4971.709 kb on - strand at 4971.746 kb on + strand, within Pf1N1B4_4509 at 4971.746 kb on + strand, within Pf1N1B4_4509 at 4971.945 kb on + strand, within Pf1N1B4_4509 at 4971.945 kb on + strand, within Pf1N1B4_4509 at 4972.834 kb on + strand, within Pf1N1B4_4510 at 4972.834 kb on + strand, within Pf1N1B4_4510 at 4972.834 kb on + strand at 4973.249 kb on - strand at 4973.352 kb on + strand at 4973.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,971,705 + +0.0 4,971,708 + +0.3 4,971,708 + -0.7 4,971,709 - +0.8 4,971,746 + Pf1N1B4_4509 0.12 -0.4 4,971,746 + Pf1N1B4_4509 0.12 -1.4 4,971,945 + Pf1N1B4_4509 0.38 -0.4 4,971,945 + Pf1N1B4_4509 0.38 -0.9 4,972,834 + Pf1N1B4_4510 0.45 -0.4 4,972,834 + Pf1N1B4_4510 0.45 -0.3 4,972,834 + +2.3 4,973,249 - +0.3 4,973,352 + +0.3 4,973,417 - +0.0
Or see this region's nucleotide sequence