Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4244 and Pf1N1B4_4245 are separated by 7 nucleotides Pf1N1B4_4245 and Pf1N1B4_4246 are separated by 227 nucleotides
Pf1N1B4_4244 - Aldehyde dehydrogenase (EC 1.2.1.3), at 4,703,537 to 4,704,928
_4244
Pf1N1B4_4245 - glutamine synthetase family protein, at 4,704,936 to 4,706,309
_4245
Pf1N1B4_4246 - L-lactate permease, at 4,706,537 to 4,708,294
_4246
Position (kb)
4704
4705
4706
4707 Strain fitness (log2 ratio)
-2
-1
0
1 at 4704.005 kb on - strand, within Pf1N1B4_4244 at 4704.054 kb on + strand, within Pf1N1B4_4244 at 4704.055 kb on - strand, within Pf1N1B4_4244 at 4704.115 kb on - strand, within Pf1N1B4_4244 at 4704.118 kb on + strand, within Pf1N1B4_4244 at 4704.118 kb on + strand, within Pf1N1B4_4244 at 4704.118 kb on + strand, within Pf1N1B4_4244 at 4704.119 kb on - strand, within Pf1N1B4_4244 at 4704.119 kb on - strand, within Pf1N1B4_4244 at 4704.214 kb on - strand, within Pf1N1B4_4244 at 4704.503 kb on - strand, within Pf1N1B4_4244 at 4704.526 kb on - strand, within Pf1N1B4_4244 at 4704.526 kb on - strand, within Pf1N1B4_4244 at 4705.021 kb on + strand at 4705.022 kb on - strand at 4705.022 kb on - strand at 4705.022 kb on - strand at 4705.133 kb on - strand at 4705.133 kb on - strand, within Pf1N1B4_4245 at 4705.220 kb on - strand, within Pf1N1B4_4245 at 4705.320 kb on + strand, within Pf1N1B4_4245 at 4705.364 kb on - strand, within Pf1N1B4_4245 at 4705.376 kb on - strand, within Pf1N1B4_4245 at 4705.786 kb on + strand, within Pf1N1B4_4245 at 4705.787 kb on - strand, within Pf1N1B4_4245 at 4705.798 kb on + strand, within Pf1N1B4_4245 at 4705.798 kb on + strand, within Pf1N1B4_4245 at 4705.826 kb on - strand, within Pf1N1B4_4245 at 4705.826 kb on - strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.850 kb on - strand, within Pf1N1B4_4245 at 4705.850 kb on - strand, within Pf1N1B4_4245 at 4705.867 kb on + strand, within Pf1N1B4_4245 at 4705.867 kb on + strand at 4705.868 kb on - strand, within Pf1N1B4_4245 at 4706.141 kb on - strand, within Pf1N1B4_4245 at 4706.141 kb on - strand, within Pf1N1B4_4245 at 4706.349 kb on + strand at 4706.349 kb on + strand at 4706.350 kb on - strand at 4706.350 kb on - strand at 4706.431 kb on + strand at 4706.431 kb on + strand at 4706.431 kb on + strand at 4706.432 kb on - strand at 4706.432 kb on - strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.578 kb on - strand at 4707.015 kb on + strand, within Pf1N1B4_4246 at 4707.016 kb on - strand, within Pf1N1B4_4246 at 4707.117 kb on + strand, within Pf1N1B4_4246 at 4707.117 kb on + strand, within Pf1N1B4_4246 at 4707.118 kb on - strand, within Pf1N1B4_4246 at 4707.118 kb on - strand, within Pf1N1B4_4246 at 4707.121 kb on + strand, within Pf1N1B4_4246 at 4707.122 kb on - strand, within Pf1N1B4_4246
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,704,005 - Pf1N1B4_4244 0.34 +0.9 4,704,054 + Pf1N1B4_4244 0.37 -0.8 4,704,055 - Pf1N1B4_4244 0.37 -0.6 4,704,115 - Pf1N1B4_4244 0.42 -0.9 4,704,118 + Pf1N1B4_4244 0.42 +0.0 4,704,118 + Pf1N1B4_4244 0.42 -0.6 4,704,118 + Pf1N1B4_4244 0.42 -1.0 4,704,119 - Pf1N1B4_4244 0.42 -0.1 4,704,119 - Pf1N1B4_4244 0.42 +0.1 4,704,214 - Pf1N1B4_4244 0.49 -0.8 4,704,503 - Pf1N1B4_4244 0.69 +0.3 4,704,526 - Pf1N1B4_4244 0.71 -0.6 4,704,526 - Pf1N1B4_4244 0.71 +0.6 4,705,021 + +0.8 4,705,022 - -0.6 4,705,022 - -2.1 4,705,022 - -0.4 4,705,133 - -0.1 4,705,133 - Pf1N1B4_4245 0.14 -0.9 4,705,220 - Pf1N1B4_4245 0.21 -0.1 4,705,320 + Pf1N1B4_4245 0.28 -0.9 4,705,364 - Pf1N1B4_4245 0.31 -1.1 4,705,376 - Pf1N1B4_4245 0.32 -1.4 4,705,786 + Pf1N1B4_4245 0.62 -1.9 4,705,787 - Pf1N1B4_4245 0.62 -0.9 4,705,798 + Pf1N1B4_4245 0.63 -1.0 4,705,798 + Pf1N1B4_4245 0.63 +0.2 4,705,826 - Pf1N1B4_4245 0.65 -1.1 4,705,826 - Pf1N1B4_4245 0.65 +1.4 4,705,849 + Pf1N1B4_4245 0.66 +0.0 4,705,849 + Pf1N1B4_4245 0.66 -0.6 4,705,849 + Pf1N1B4_4245 0.66 -1.0 4,705,849 + Pf1N1B4_4245 0.66 +0.9 4,705,850 - Pf1N1B4_4245 0.67 -1.9 4,705,850 - Pf1N1B4_4245 0.67 -0.9 4,705,867 + Pf1N1B4_4245 0.68 +0.4 4,705,867 + +0.4 4,705,868 - Pf1N1B4_4245 0.68 -1.7 4,706,141 - Pf1N1B4_4245 0.88 -0.7 4,706,141 - Pf1N1B4_4245 0.88 +0.9 4,706,349 + +0.4 4,706,349 + -1.6 4,706,350 - -1.4 4,706,350 - -0.6 4,706,431 + +0.2 4,706,431 + +0.8 4,706,431 + -2.0 4,706,432 - -0.6 4,706,432 - +0.6 4,706,577 + +0.4 4,706,577 + +0.3 4,706,577 + -0.0 4,706,577 + -0.0 4,706,577 + +0.2 4,706,577 + -0.2 4,706,578 - -0.2 4,707,015 + Pf1N1B4_4246 0.27 -0.1 4,707,016 - Pf1N1B4_4246 0.27 -0.1 4,707,117 + Pf1N1B4_4246 0.33 +0.4 4,707,117 + Pf1N1B4_4246 0.33 +0.1 4,707,118 - Pf1N1B4_4246 0.33 -0.1 4,707,118 - Pf1N1B4_4246 0.33 -0.2 4,707,121 + Pf1N1B4_4246 0.33 +1.2 4,707,122 - Pf1N1B4_4246 0.33 +0.3
Or see this region's nucleotide sequence