Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4223 and Pf1N1B4_4224 are separated by 306 nucleotides Pf1N1B4_4224 and Pf1N1B4_4225 are separated by 149 nucleotides Pf1N1B4_4225 and Pf1N1B4_4226 are separated by 79 nucleotides
Pf1N1B4_4223 - Transcriptional regulator, at 4,679,386 to 4,680,279
_4223
Pf1N1B4_4224 - putative tautomerase, at 4,680,586 to 4,680,795
_4224
Pf1N1B4_4225 - transcriptional regulator, AraC family, at 4,680,945 to 4,681,907
_4225
Pf1N1B4_4226 - Sterol desaturase family protein, at 4,681,987 to 4,682,829
_4226
Position (kb)
4680
4681
4682 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4680.137 kb on - strand, within Pf1N1B4_4223 at 4680.244 kb on - strand at 4680.303 kb on + strand at 4680.380 kb on - strand at 4680.403 kb on - strand at 4680.433 kb on + strand at 4680.433 kb on + strand at 4680.437 kb on + strand at 4680.437 kb on + strand at 4680.438 kb on - strand at 4680.542 kb on + strand at 4680.793 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.947 kb on - strand at 4680.982 kb on + strand at 4680.982 kb on + strand at 4681.083 kb on + strand at 4681.083 kb on + strand, within Pf1N1B4_4225 at 4681.396 kb on + strand, within Pf1N1B4_4225 at 4681.405 kb on + strand, within Pf1N1B4_4225 at 4681.499 kb on - strand, within Pf1N1B4_4225 at 4681.657 kb on + strand, within Pf1N1B4_4225 at 4681.658 kb on - strand, within Pf1N1B4_4225 at 4681.669 kb on + strand at 4681.670 kb on - strand, within Pf1N1B4_4225 at 4681.670 kb on - strand at 4681.772 kb on + strand, within Pf1N1B4_4225 at 4681.882 kb on - strand at 4681.882 kb on - strand at 4681.980 kb on + strand at 4681.980 kb on + strand at 4681.982 kb on + strand at 4681.983 kb on - strand at 4682.148 kb on - strand, within Pf1N1B4_4226 at 4682.172 kb on - strand, within Pf1N1B4_4226 at 4682.246 kb on + strand, within Pf1N1B4_4226 at 4682.270 kb on - strand, within Pf1N1B4_4226 at 4682.341 kb on + strand, within Pf1N1B4_4226 at 4682.342 kb on - strand, within Pf1N1B4_4226 at 4682.342 kb on - strand at 4682.343 kb on + strand at 4682.344 kb on - strand, within Pf1N1B4_4226 at 4682.344 kb on - strand, within Pf1N1B4_4226 at 4682.344 kb on - strand, within Pf1N1B4_4226 at 4682.376 kb on + strand, within Pf1N1B4_4226 at 4682.377 kb on - strand, within Pf1N1B4_4226 at 4682.381 kb on - strand, within Pf1N1B4_4226 at 4682.383 kb on + strand, within Pf1N1B4_4226 at 4682.384 kb on - strand, within Pf1N1B4_4226 at 4682.425 kb on + strand, within Pf1N1B4_4226 at 4682.426 kb on - strand, within Pf1N1B4_4226 at 4682.426 kb on - strand, within Pf1N1B4_4226 at 4682.426 kb on - strand, within Pf1N1B4_4226 at 4682.447 kb on - strand, within Pf1N1B4_4226 at 4682.449 kb on + strand, within Pf1N1B4_4226 at 4682.450 kb on - strand, within Pf1N1B4_4226 at 4682.632 kb on + strand, within Pf1N1B4_4226 at 4682.633 kb on - strand, within Pf1N1B4_4226 at 4682.759 kb on + strand at 4682.759 kb on + strand at 4682.760 kb on - strand at 4682.760 kb on - strand at 4682.801 kb on + strand at 4682.837 kb on + strand at 4682.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,680,137 - Pf1N1B4_4223 0.84 +0.9 4,680,244 - -0.6 4,680,303 + +2.0 4,680,380 - +0.4 4,680,403 - -1.0 4,680,433 + -1.1 4,680,433 + -0.3 4,680,437 + -0.8 4,680,437 + -0.6 4,680,438 - +0.9 4,680,542 + +0.2 4,680,793 + -0.1 4,680,946 + -2.7 4,680,946 + -0.4 4,680,946 + +0.4 4,680,947 - +0.4 4,680,982 + +1.0 4,680,982 + -1.4 4,681,083 + +0.0 4,681,083 + Pf1N1B4_4225 0.14 +0.4 4,681,396 + Pf1N1B4_4225 0.47 -0.7 4,681,405 + Pf1N1B4_4225 0.48 -0.6 4,681,499 - Pf1N1B4_4225 0.58 -2.2 4,681,657 + Pf1N1B4_4225 0.74 -0.7 4,681,658 - Pf1N1B4_4225 0.74 -1.1 4,681,669 + -1.1 4,681,670 - Pf1N1B4_4225 0.75 -1.0 4,681,670 - -0.6 4,681,772 + Pf1N1B4_4225 0.86 +0.9 4,681,882 - -1.3 4,681,882 - -0.2 4,681,980 + -0.3 4,681,980 + +0.1 4,681,982 + -0.9 4,681,983 - +0.4 4,682,148 - Pf1N1B4_4226 0.19 +0.9 4,682,172 - Pf1N1B4_4226 0.22 +0.2 4,682,246 + Pf1N1B4_4226 0.31 -0.3 4,682,270 - Pf1N1B4_4226 0.34 +0.4 4,682,341 + Pf1N1B4_4226 0.42 +0.9 4,682,342 - Pf1N1B4_4226 0.42 -0.8 4,682,342 - +1.2 4,682,343 + +1.8 4,682,344 - Pf1N1B4_4226 0.42 +1.8 4,682,344 - Pf1N1B4_4226 0.42 -1.3 4,682,344 - Pf1N1B4_4226 0.42 -1.4 4,682,376 + Pf1N1B4_4226 0.46 +0.6 4,682,377 - Pf1N1B4_4226 0.46 +0.4 4,682,381 - Pf1N1B4_4226 0.47 +1.2 4,682,383 + Pf1N1B4_4226 0.47 -1.6 4,682,384 - Pf1N1B4_4226 0.47 -0.1 4,682,425 + Pf1N1B4_4226 0.52 -0.6 4,682,426 - Pf1N1B4_4226 0.52 +0.1 4,682,426 - Pf1N1B4_4226 0.52 -0.3 4,682,426 - Pf1N1B4_4226 0.52 +2.3 4,682,447 - Pf1N1B4_4226 0.55 +0.1 4,682,449 + Pf1N1B4_4226 0.55 -0.7 4,682,450 - Pf1N1B4_4226 0.55 +0.9 4,682,632 + Pf1N1B4_4226 0.77 +0.3 4,682,633 - Pf1N1B4_4226 0.77 -0.0 4,682,759 + +1.4 4,682,759 + +0.7 4,682,760 - +0.4 4,682,760 - -0.4 4,682,801 + -0.1 4,682,837 + -1.0 4,682,838 - +0.3
Or see this region's nucleotide sequence