Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4165 and Pf1N1B4_4166 are separated by 130 nucleotides Pf1N1B4_4166 and Pf1N1B4_4167 are separated by 122 nucleotides Pf1N1B4_4167 and Pf1N1B4_4168 overlap by 1 nucleotides Pf1N1B4_4168 and Pf1N1B4_4169 are separated by 1 nucleotides
Pf1N1B4_4165 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 4,615,351 to 4,616,787
_4165
Pf1N1B4_4166 - Transcriptional regulator, LysR family, at 4,616,918 to 4,617,823
_4166
Pf1N1B4_4167 - hypothetical protein, at 4,617,946 to 4,618,296
_4167
Pf1N1B4_4168 - putative membrane protein, at 4,618,296 to 4,618,808
_4168
Pf1N1B4_4169 - Hydrolases of the alpha/beta superfamily, at 4,618,810 to 4,619,559
_4169
Position (kb)
4616
4617
4618 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4615.928 kb on - strand at 4616.093 kb on + strand, within Pf1N1B4_4165 at 4616.094 kb on - strand, within Pf1N1B4_4165 at 4616.097 kb on - strand at 4616.177 kb on - strand at 4616.194 kb on + strand, within Pf1N1B4_4165 at 4616.195 kb on - strand, within Pf1N1B4_4165 at 4616.195 kb on - strand, within Pf1N1B4_4165 at 4616.261 kb on + strand, within Pf1N1B4_4165 at 4616.261 kb on + strand, within Pf1N1B4_4165 at 4616.262 kb on - strand, within Pf1N1B4_4165 at 4616.762 kb on + strand at 4616.762 kb on + strand at 4616.763 kb on - strand at 4616.763 kb on - strand at 4616.767 kb on + strand at 4616.768 kb on - strand at 4616.768 kb on - strand at 4616.771 kb on + strand at 4616.771 kb on + strand at 4616.772 kb on - strand at 4616.772 kb on - strand at 4616.817 kb on + strand at 4616.817 kb on + strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4616.818 kb on - strand at 4617.416 kb on + strand, within Pf1N1B4_4166 at 4617.416 kb on + strand, within Pf1N1B4_4166 at 4617.432 kb on - strand, within Pf1N1B4_4166 at 4617.432 kb on - strand, within Pf1N1B4_4166 at 4617.521 kb on + strand, within Pf1N1B4_4166 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.422 kb on - strand at 4618.422 kb on - strand at 4618.422 kb on - strand at 4618.422 kb on - strand, within Pf1N1B4_4168 at 4618.493 kb on + strand, within Pf1N1B4_4168 at 4618.800 kb on + strand at 4618.800 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,615,928 - +1.5 4,616,093 + Pf1N1B4_4165 0.52 +0.7 4,616,094 - Pf1N1B4_4165 0.52 +0.2 4,616,097 - +1.5 4,616,177 - +2.0 4,616,194 + Pf1N1B4_4165 0.59 +0.5 4,616,195 - Pf1N1B4_4165 0.59 +1.2 4,616,195 - Pf1N1B4_4165 0.59 -2.3 4,616,261 + Pf1N1B4_4165 0.63 -0.0 4,616,261 + Pf1N1B4_4165 0.63 -1.1 4,616,262 - Pf1N1B4_4165 0.63 +0.2 4,616,762 + -0.3 4,616,762 + -0.3 4,616,763 - -0.2 4,616,763 - +0.7 4,616,767 + +2.0 4,616,768 - +0.2 4,616,768 - +0.2 4,616,771 + -0.1 4,616,771 + +0.5 4,616,772 - -0.8 4,616,772 - +0.5 4,616,817 + -0.2 4,616,817 + -0.7 4,616,818 - +0.0 4,616,818 - -0.2 4,616,818 - -0.5 4,616,818 - -0.3 4,616,818 - -0.5 4,616,818 - -0.3 4,617,416 + Pf1N1B4_4166 0.55 -1.9 4,617,416 + Pf1N1B4_4166 0.55 +0.5 4,617,432 - Pf1N1B4_4166 0.57 -0.0 4,617,432 - Pf1N1B4_4166 0.57 -0.9 4,617,521 + Pf1N1B4_4166 0.67 -1.3 4,618,421 + Pf1N1B4_4168 0.24 +1.0 4,618,421 + Pf1N1B4_4168 0.24 -1.1 4,618,421 + Pf1N1B4_4168 0.24 -0.1 4,618,422 - -0.1 4,618,422 - +0.5 4,618,422 - -0.5 4,618,422 - Pf1N1B4_4168 0.25 -0.0 4,618,493 + Pf1N1B4_4168 0.38 -0.5 4,618,800 + -0.3 4,618,800 + -0.5
Or see this region's nucleotide sequence