Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4150

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4149 and Pf1N1B4_4150 are separated by 257 nucleotidesPf1N1B4_4150 and Pf1N1B4_4151 are separated by 81 nucleotidesPf1N1B4_4151 and Pf1N1B4_4152 are separated by 24 nucleotidesPf1N1B4_4152 and Pf1N1B4_4153 are separated by 177 nucleotides Pf1N1B4_4149 - Probable lipoprotein signal peptide, at 4,594,744 to 4,595,796 _4149 Pf1N1B4_4150 - Transcriptional regulator, AraC family, at 4,596,054 to 4,596,971 _4150 Pf1N1B4_4151 - FIG00960153: hypothetical protein, at 4,597,053 to 4,597,310 _4151 Pf1N1B4_4152 - FIG00963947: hypothetical protein, at 4,597,335 to 4,597,625 _4152 Pf1N1B4_4153 - Glucose 1-dehydrogenase (EC 1.1.1.47), at 4,597,803 to 4,598,567 _4153 Position (kb) 4596 4597Strain fitness (log2 ratio) -2 -1 0 1 2at 4595.062 kb on + strand, within Pf1N1B4_4149at 4595.062 kb on + strand, within Pf1N1B4_4149at 4595.062 kb on + strand, within Pf1N1B4_4149at 4595.063 kb on - strand, within Pf1N1B4_4149at 4595.132 kb on + strand, within Pf1N1B4_4149at 4595.342 kb on - strand, within Pf1N1B4_4149at 4595.365 kb on - strandat 4595.375 kb on + strandat 4595.495 kb on + strandat 4595.495 kb on + strand, within Pf1N1B4_4149at 4595.496 kb on - strand, within Pf1N1B4_4149at 4595.496 kb on - strand, within Pf1N1B4_4149at 4595.496 kb on - strand, within Pf1N1B4_4149at 4595.723 kb on + strandat 4595.740 kb on - strandat 4595.740 kb on - strandat 4595.795 kb on - strandat 4595.795 kb on - strandat 4595.795 kb on - strandat 4595.872 kb on + strandat 4595.872 kb on + strandat 4595.873 kb on - strandat 4595.873 kb on - strandat 4595.873 kb on - strandat 4595.876 kb on - strandat 4595.893 kb on + strandat 4595.894 kb on - strandat 4595.894 kb on - strandat 4595.945 kb on + strandat 4595.945 kb on + strandat 4595.977 kb on + strandat 4595.978 kb on - strandat 4595.978 kb on - strandat 4595.992 kb on + strandat 4595.993 kb on - strandat 4595.996 kb on + strandat 4595.996 kb on + strandat 4595.996 kb on + strandat 4595.997 kb on - strandat 4595.997 kb on - strandat 4596.001 kb on + strandat 4596.007 kb on + strandat 4596.008 kb on - strandat 4596.010 kb on + strandat 4596.019 kb on - strandat 4596.019 kb on - strandat 4596.029 kb on - strandat 4596.406 kb on + strand, within Pf1N1B4_4150at 4596.445 kb on + strand, within Pf1N1B4_4150at 4596.445 kb on + strand, within Pf1N1B4_4150at 4596.446 kb on - strand, within Pf1N1B4_4150at 4596.446 kb on - strand, within Pf1N1B4_4150at 4596.446 kb on - strand, within Pf1N1B4_4150at 4596.655 kb on + strand, within Pf1N1B4_4150at 4596.721 kb on + strand, within Pf1N1B4_4150at 4596.721 kb on + strand, within Pf1N1B4_4150at 4596.722 kb on - strand, within Pf1N1B4_4150at 4596.874 kb on + strand, within Pf1N1B4_4150at 4596.874 kb on + strand, within Pf1N1B4_4150at 4596.874 kb on + strand, within Pf1N1B4_4150at 4596.874 kb on + strandat 4596.875 kb on - strand, within Pf1N1B4_4150at 4596.875 kb on - strand, within Pf1N1B4_4150at 4596.929 kb on - strandat 4596.929 kb on - strandat 4597.085 kb on - strand, within Pf1N1B4_4151at 4597.147 kb on + strand, within Pf1N1B4_4151at 4597.147 kb on + strandat 4597.148 kb on - strand, within Pf1N1B4_4151at 4597.148 kb on - strand, within Pf1N1B4_4151at 4597.148 kb on - strand, within Pf1N1B4_4151at 4597.159 kb on - strand, within Pf1N1B4_4151at 4597.187 kb on + strand, within Pf1N1B4_4151at 4597.188 kb on - strand, within Pf1N1B4_4151at 4597.188 kb on - strand, within Pf1N1B4_4151at 4597.188 kb on - strand, within Pf1N1B4_4151at 4597.336 kb on + strandat 4597.365 kb on - strand, within Pf1N1B4_4152at 4597.541 kb on - strand, within Pf1N1B4_4152at 4597.541 kb on - strand, within Pf1N1B4_4152at 4597.695 kb on - strandat 4597.703 kb on + strandat 4597.704 kb on - strandat 4597.704 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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4,595,062 + Pf1N1B4_4149 0.30 +1.5
4,595,062 + Pf1N1B4_4149 0.30 +0.2
4,595,062 + Pf1N1B4_4149 0.30 +1.5
4,595,063 - Pf1N1B4_4149 0.30 -0.1
4,595,132 + Pf1N1B4_4149 0.37 -0.7
4,595,342 - Pf1N1B4_4149 0.57 +0.2
4,595,365 - +1.0
4,595,375 + +0.5
4,595,495 + +0.5
4,595,495 + Pf1N1B4_4149 0.71 -0.3
4,595,496 - Pf1N1B4_4149 0.71 +1.1
4,595,496 - Pf1N1B4_4149 0.71 -1.3
4,595,496 - Pf1N1B4_4149 0.71 -0.4
4,595,723 + -1.1
4,595,740 - -1.1
4,595,740 - -0.8
4,595,795 - -0.3
4,595,795 - +1.8
4,595,795 - +0.5
4,595,872 + +0.5
4,595,872 + +0.5
4,595,873 - -0.3
4,595,873 - -0.3
4,595,873 - +0.2
4,595,876 - -2.7
4,595,893 + -1.2
4,595,894 - -1.1
4,595,894 - -1.3
4,595,945 + -0.3
4,595,945 + +0.5
4,595,977 + -0.1
4,595,978 - -0.3
4,595,978 - +0.8
4,595,992 + -0.1
4,595,993 - +0.2
4,595,996 + -0.3
4,595,996 + -0.9
4,595,996 + -1.3
4,595,997 - +0.9
4,595,997 - -1.0
4,596,001 + -0.3
4,596,007 + +0.5
4,596,008 - +0.1
4,596,010 + -0.0
4,596,019 - +0.9
4,596,019 - -0.1
4,596,029 - +0.2
4,596,406 + Pf1N1B4_4150 0.38 -0.4
4,596,445 + Pf1N1B4_4150 0.43 -0.7
4,596,445 + Pf1N1B4_4150 0.43 -0.5
4,596,446 - Pf1N1B4_4150 0.43 -0.7
4,596,446 - Pf1N1B4_4150 0.43 -0.3
4,596,446 - Pf1N1B4_4150 0.43 -0.8
4,596,655 + Pf1N1B4_4150 0.65 +0.5
4,596,721 + Pf1N1B4_4150 0.73 -0.3
4,596,721 + Pf1N1B4_4150 0.73 -1.3
4,596,722 - Pf1N1B4_4150 0.73 -0.9
4,596,874 + Pf1N1B4_4150 0.89 -0.1
4,596,874 + Pf1N1B4_4150 0.89 -0.5
4,596,874 + Pf1N1B4_4150 0.89 -1.4
4,596,874 + +0.5
4,596,875 - Pf1N1B4_4150 0.89 -2.1
4,596,875 - Pf1N1B4_4150 0.89 -2.3
4,596,929 - -0.5
4,596,929 - -0.8
4,597,085 - Pf1N1B4_4151 0.12 +0.7
4,597,147 + Pf1N1B4_4151 0.36 -0.3
4,597,147 + +0.0
4,597,148 - Pf1N1B4_4151 0.37 -0.5
4,597,148 - Pf1N1B4_4151 0.37 +0.0
4,597,148 - Pf1N1B4_4151 0.37 -0.2
4,597,159 - Pf1N1B4_4151 0.41 -0.6
4,597,187 + Pf1N1B4_4151 0.52 -0.9
4,597,188 - Pf1N1B4_4151 0.52 +1.0
4,597,188 - Pf1N1B4_4151 0.52 -2.0
4,597,188 - Pf1N1B4_4151 0.52 -0.1
4,597,336 + +0.2
4,597,365 - Pf1N1B4_4152 0.10 -1.0
4,597,541 - Pf1N1B4_4152 0.71 +1.0
4,597,541 - Pf1N1B4_4152 0.71 +0.6
4,597,695 - -0.4
4,597,703 + +0.5
4,597,704 - -0.5
4,597,704 - +0.2

Or see this region's nucleotide sequence