Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4149 and Pf1N1B4_4150 are separated by 257 nucleotides Pf1N1B4_4150 and Pf1N1B4_4151 are separated by 81 nucleotides Pf1N1B4_4151 and Pf1N1B4_4152 are separated by 24 nucleotides Pf1N1B4_4152 and Pf1N1B4_4153 are separated by 177 nucleotides
Pf1N1B4_4149 - Probable lipoprotein signal peptide, at 4,594,744 to 4,595,796
_4149
Pf1N1B4_4150 - Transcriptional regulator, AraC family, at 4,596,054 to 4,596,971
_4150
Pf1N1B4_4151 - FIG00960153: hypothetical protein, at 4,597,053 to 4,597,310
_4151
Pf1N1B4_4152 - FIG00963947: hypothetical protein, at 4,597,335 to 4,597,625
_4152
Pf1N1B4_4153 - Glucose 1-dehydrogenase (EC 1.1.1.47), at 4,597,803 to 4,598,567
_4153
Position (kb)
4596
4597 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4595.062 kb on + strand, within Pf1N1B4_4149 at 4595.062 kb on + strand, within Pf1N1B4_4149 at 4595.062 kb on + strand, within Pf1N1B4_4149 at 4595.063 kb on - strand, within Pf1N1B4_4149 at 4595.132 kb on + strand, within Pf1N1B4_4149 at 4595.342 kb on - strand, within Pf1N1B4_4149 at 4595.365 kb on - strand at 4595.375 kb on + strand at 4595.495 kb on + strand at 4595.495 kb on + strand, within Pf1N1B4_4149 at 4595.496 kb on - strand, within Pf1N1B4_4149 at 4595.496 kb on - strand, within Pf1N1B4_4149 at 4595.496 kb on - strand, within Pf1N1B4_4149 at 4595.723 kb on + strand at 4595.740 kb on - strand at 4595.740 kb on - strand at 4595.795 kb on - strand at 4595.795 kb on - strand at 4595.795 kb on - strand at 4595.872 kb on + strand at 4595.872 kb on + strand at 4595.873 kb on - strand at 4595.873 kb on - strand at 4595.873 kb on - strand at 4595.876 kb on - strand at 4595.893 kb on + strand at 4595.894 kb on - strand at 4595.894 kb on - strand at 4595.945 kb on + strand at 4595.945 kb on + strand at 4595.977 kb on + strand at 4595.978 kb on - strand at 4595.978 kb on - strand at 4595.992 kb on + strand at 4595.993 kb on - strand at 4595.996 kb on + strand at 4595.996 kb on + strand at 4595.996 kb on + strand at 4595.997 kb on - strand at 4595.997 kb on - strand at 4596.001 kb on + strand at 4596.007 kb on + strand at 4596.008 kb on - strand at 4596.010 kb on + strand at 4596.019 kb on - strand at 4596.019 kb on - strand at 4596.029 kb on - strand at 4596.406 kb on + strand, within Pf1N1B4_4150 at 4596.445 kb on + strand, within Pf1N1B4_4150 at 4596.445 kb on + strand, within Pf1N1B4_4150 at 4596.446 kb on - strand, within Pf1N1B4_4150 at 4596.446 kb on - strand, within Pf1N1B4_4150 at 4596.446 kb on - strand, within Pf1N1B4_4150 at 4596.655 kb on + strand, within Pf1N1B4_4150 at 4596.721 kb on + strand, within Pf1N1B4_4150 at 4596.721 kb on + strand, within Pf1N1B4_4150 at 4596.722 kb on - strand, within Pf1N1B4_4150 at 4596.874 kb on + strand, within Pf1N1B4_4150 at 4596.874 kb on + strand, within Pf1N1B4_4150 at 4596.874 kb on + strand, within Pf1N1B4_4150 at 4596.874 kb on + strand at 4596.875 kb on - strand, within Pf1N1B4_4150 at 4596.875 kb on - strand, within Pf1N1B4_4150 at 4596.929 kb on - strand at 4596.929 kb on - strand at 4597.085 kb on - strand, within Pf1N1B4_4151 at 4597.147 kb on + strand, within Pf1N1B4_4151 at 4597.147 kb on + strand at 4597.148 kb on - strand, within Pf1N1B4_4151 at 4597.148 kb on - strand, within Pf1N1B4_4151 at 4597.148 kb on - strand, within Pf1N1B4_4151 at 4597.159 kb on - strand, within Pf1N1B4_4151 at 4597.187 kb on + strand, within Pf1N1B4_4151 at 4597.188 kb on - strand, within Pf1N1B4_4151 at 4597.188 kb on - strand, within Pf1N1B4_4151 at 4597.188 kb on - strand, within Pf1N1B4_4151 at 4597.336 kb on + strand at 4597.365 kb on - strand, within Pf1N1B4_4152 at 4597.541 kb on - strand, within Pf1N1B4_4152 at 4597.541 kb on - strand, within Pf1N1B4_4152 at 4597.695 kb on - strand at 4597.703 kb on + strand at 4597.704 kb on - strand at 4597.704 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,595,062 + Pf1N1B4_4149 0.30 +1.5 4,595,062 + Pf1N1B4_4149 0.30 +0.2 4,595,062 + Pf1N1B4_4149 0.30 +1.5 4,595,063 - Pf1N1B4_4149 0.30 -0.1 4,595,132 + Pf1N1B4_4149 0.37 -0.7 4,595,342 - Pf1N1B4_4149 0.57 +0.2 4,595,365 - +1.0 4,595,375 + +0.5 4,595,495 + +0.5 4,595,495 + Pf1N1B4_4149 0.71 -0.3 4,595,496 - Pf1N1B4_4149 0.71 +1.1 4,595,496 - Pf1N1B4_4149 0.71 -1.3 4,595,496 - Pf1N1B4_4149 0.71 -0.4 4,595,723 + -1.1 4,595,740 - -1.1 4,595,740 - -0.8 4,595,795 - -0.3 4,595,795 - +1.8 4,595,795 - +0.5 4,595,872 + +0.5 4,595,872 + +0.5 4,595,873 - -0.3 4,595,873 - -0.3 4,595,873 - +0.2 4,595,876 - -2.7 4,595,893 + -1.2 4,595,894 - -1.1 4,595,894 - -1.3 4,595,945 + -0.3 4,595,945 + +0.5 4,595,977 + -0.1 4,595,978 - -0.3 4,595,978 - +0.8 4,595,992 + -0.1 4,595,993 - +0.2 4,595,996 + -0.3 4,595,996 + -0.9 4,595,996 + -1.3 4,595,997 - +0.9 4,595,997 - -1.0 4,596,001 + -0.3 4,596,007 + +0.5 4,596,008 - +0.1 4,596,010 + -0.0 4,596,019 - +0.9 4,596,019 - -0.1 4,596,029 - +0.2 4,596,406 + Pf1N1B4_4150 0.38 -0.4 4,596,445 + Pf1N1B4_4150 0.43 -0.7 4,596,445 + Pf1N1B4_4150 0.43 -0.5 4,596,446 - Pf1N1B4_4150 0.43 -0.7 4,596,446 - Pf1N1B4_4150 0.43 -0.3 4,596,446 - Pf1N1B4_4150 0.43 -0.8 4,596,655 + Pf1N1B4_4150 0.65 +0.5 4,596,721 + Pf1N1B4_4150 0.73 -0.3 4,596,721 + Pf1N1B4_4150 0.73 -1.3 4,596,722 - Pf1N1B4_4150 0.73 -0.9 4,596,874 + Pf1N1B4_4150 0.89 -0.1 4,596,874 + Pf1N1B4_4150 0.89 -0.5 4,596,874 + Pf1N1B4_4150 0.89 -1.4 4,596,874 + +0.5 4,596,875 - Pf1N1B4_4150 0.89 -2.1 4,596,875 - Pf1N1B4_4150 0.89 -2.3 4,596,929 - -0.5 4,596,929 - -0.8 4,597,085 - Pf1N1B4_4151 0.12 +0.7 4,597,147 + Pf1N1B4_4151 0.36 -0.3 4,597,147 + +0.0 4,597,148 - Pf1N1B4_4151 0.37 -0.5 4,597,148 - Pf1N1B4_4151 0.37 +0.0 4,597,148 - Pf1N1B4_4151 0.37 -0.2 4,597,159 - Pf1N1B4_4151 0.41 -0.6 4,597,187 + Pf1N1B4_4151 0.52 -0.9 4,597,188 - Pf1N1B4_4151 0.52 +1.0 4,597,188 - Pf1N1B4_4151 0.52 -2.0 4,597,188 - Pf1N1B4_4151 0.52 -0.1 4,597,336 + +0.2 4,597,365 - Pf1N1B4_4152 0.10 -1.0 4,597,541 - Pf1N1B4_4152 0.71 +1.0 4,597,541 - Pf1N1B4_4152 0.71 +0.6 4,597,695 - -0.4 4,597,703 + +0.5 4,597,704 - -0.5 4,597,704 - +0.2
Or see this region's nucleotide sequence