Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4135 and Pf1N1B4_4136 are separated by 505 nucleotides
Pf1N1B4_4135 - hypothetical protein, at 4,577,414 to 4,578,589
_4135
Pf1N1B4_4136 - hypothetical protein, at 4,579,095 to 4,579,418
_4136
Position (kb)
4577
4578
4579 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4576.518 kb on + strand at 4576.544 kb on + strand at 4576.545 kb on - strand at 4576.691 kb on + strand at 4576.691 kb on + strand at 4576.692 kb on - strand at 4576.692 kb on - strand at 4576.736 kb on + strand at 4576.737 kb on - strand at 4576.769 kb on - strand at 4576.771 kb on + strand at 4576.772 kb on - strand at 4576.906 kb on - strand at 4576.968 kb on + strand at 4576.968 kb on + strand at 4576.968 kb on + strand at 4577.145 kb on + strand at 4577.156 kb on + strand at 4577.218 kb on - strand at 4577.220 kb on + strand at 4577.230 kb on + strand at 4577.230 kb on + strand at 4577.232 kb on + strand at 4577.316 kb on + strand at 4577.316 kb on + strand at 4577.317 kb on - strand at 4577.317 kb on - strand at 4577.397 kb on - strand at 4577.549 kb on + strand, within Pf1N1B4_4135 at 4577.549 kb on + strand at 4577.578 kb on + strand, within Pf1N1B4_4135 at 4577.634 kb on + strand, within Pf1N1B4_4135 at 4577.654 kb on - strand, within Pf1N1B4_4135 at 4577.654 kb on - strand, within Pf1N1B4_4135 at 4577.683 kb on + strand at 4577.701 kb on + strand, within Pf1N1B4_4135 at 4577.713 kb on + strand, within Pf1N1B4_4135 at 4577.713 kb on + strand, within Pf1N1B4_4135 at 4577.741 kb on - strand, within Pf1N1B4_4135 at 4577.768 kb on + strand, within Pf1N1B4_4135 at 4577.846 kb on - strand, within Pf1N1B4_4135 at 4577.977 kb on + strand, within Pf1N1B4_4135 at 4577.977 kb on + strand at 4578.064 kb on - strand, within Pf1N1B4_4135 at 4578.088 kb on + strand, within Pf1N1B4_4135 at 4578.185 kb on + strand, within Pf1N1B4_4135 at 4578.185 kb on + strand, within Pf1N1B4_4135 at 4578.223 kb on - strand, within Pf1N1B4_4135 at 4578.274 kb on - strand, within Pf1N1B4_4135 at 4578.301 kb on + strand, within Pf1N1B4_4135 at 4578.451 kb on + strand at 4578.451 kb on + strand, within Pf1N1B4_4135 at 4578.452 kb on - strand, within Pf1N1B4_4135 at 4578.479 kb on - strand at 4578.631 kb on + strand at 4578.636 kb on - strand at 4578.683 kb on + strand at 4578.683 kb on + strand at 4578.748 kb on - strand at 4578.760 kb on + strand at 4578.760 kb on + strand at 4578.760 kb on + strand at 4578.761 kb on - strand at 4578.900 kb on + strand at 4578.900 kb on + strand at 4578.901 kb on - strand at 4578.966 kb on + strand at 4578.986 kb on + strand at 4578.987 kb on - strand at 4579.038 kb on + strand at 4579.038 kb on + strand at 4579.038 kb on + strand at 4579.038 kb on + strand at 4579.039 kb on - strand at 4579.039 kb on - strand at 4579.039 kb on - strand at 4579.040 kb on + strand at 4579.040 kb on + strand at 4579.040 kb on + strand at 4579.040 kb on + strand at 4579.040 kb on + strand at 4579.040 kb on + strand at 4579.040 kb on + strand at 4579.041 kb on - strand at 4579.041 kb on - strand at 4579.094 kb on + strand at 4579.094 kb on + strand at 4579.094 kb on + strand at 4579.094 kb on + strand at 4579.094 kb on + strand at 4579.094 kb on + strand at 4579.094 kb on + strand at 4579.095 kb on - strand at 4579.095 kb on - strand at 4579.167 kb on + strand, within Pf1N1B4_4136 at 4579.167 kb on + strand, within Pf1N1B4_4136 at 4579.167 kb on + strand, within Pf1N1B4_4136 at 4579.167 kb on + strand at 4579.168 kb on - strand, within Pf1N1B4_4136 at 4579.168 kb on - strand, within Pf1N1B4_4136 at 4579.168 kb on - strand, within Pf1N1B4_4136 at 4579.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,576,518 + -0.8 4,576,544 + +0.5 4,576,545 - -0.9 4,576,691 + -2.3 4,576,691 + -0.8 4,576,692 - -0.7 4,576,692 - -0.5 4,576,736 + -1.5 4,576,737 - -0.5 4,576,769 - -0.1 4,576,771 + +1.5 4,576,772 - +1.0 4,576,906 - +0.5 4,576,968 + +1.2 4,576,968 + -1.1 4,576,968 + +0.1 4,577,145 + +0.9 4,577,156 + +1.5 4,577,218 - -0.3 4,577,220 + -0.3 4,577,230 + +1.2 4,577,230 + +0.5 4,577,232 + -1.5 4,577,316 + -0.4 4,577,316 + -0.5 4,577,317 - -0.1 4,577,317 - +0.5 4,577,397 - -0.5 4,577,549 + Pf1N1B4_4135 0.11 -2.1 4,577,549 + -1.9 4,577,578 + Pf1N1B4_4135 0.14 -0.5 4,577,634 + Pf1N1B4_4135 0.19 -1.9 4,577,654 - Pf1N1B4_4135 0.20 -0.8 4,577,654 - Pf1N1B4_4135 0.20 +0.5 4,577,683 + -0.1 4,577,701 + Pf1N1B4_4135 0.24 -0.1 4,577,713 + Pf1N1B4_4135 0.25 -0.4 4,577,713 + Pf1N1B4_4135 0.25 +0.1 4,577,741 - Pf1N1B4_4135 0.28 -0.5 4,577,768 + Pf1N1B4_4135 0.30 -0.2 4,577,846 - Pf1N1B4_4135 0.37 -0.7 4,577,977 + Pf1N1B4_4135 0.48 -1.7 4,577,977 + +1.5 4,578,064 - Pf1N1B4_4135 0.55 +0.5 4,578,088 + Pf1N1B4_4135 0.57 +0.6 4,578,185 + Pf1N1B4_4135 0.66 -0.5 4,578,185 + Pf1N1B4_4135 0.66 -0.3 4,578,223 - Pf1N1B4_4135 0.69 -1.0 4,578,274 - Pf1N1B4_4135 0.73 +0.7 4,578,301 + Pf1N1B4_4135 0.75 -0.5 4,578,451 + +1.5 4,578,451 + Pf1N1B4_4135 0.88 -1.3 4,578,452 - Pf1N1B4_4135 0.88 -1.0 4,578,479 - -0.1 4,578,631 + +0.1 4,578,636 - -0.5 4,578,683 + +0.8 4,578,683 + -1.7 4,578,748 - -0.9 4,578,760 + -1.1 4,578,760 + -0.9 4,578,760 + -0.3 4,578,761 - +0.1 4,578,900 + -1.1 4,578,900 + +1.2 4,578,901 - -0.1 4,578,966 + -0.9 4,578,986 + +0.5 4,578,987 - +0.5 4,579,038 + -1.1 4,579,038 + -0.1 4,579,038 + -0.1 4,579,038 + +0.5 4,579,039 - +0.5 4,579,039 - -0.4 4,579,039 - +0.2 4,579,040 + +2.5 4,579,040 + -0.3 4,579,040 + +0.7 4,579,040 + +0.5 4,579,040 + +0.1 4,579,040 + +0.0 4,579,040 + +0.6 4,579,041 - -1.2 4,579,041 - -0.9 4,579,094 + +0.5 4,579,094 + +1.1 4,579,094 + -0.4 4,579,094 + +1.8 4,579,094 + +0.1 4,579,094 + -0.7 4,579,094 + +1.5 4,579,095 - -1.5 4,579,095 - -1.7 4,579,167 + Pf1N1B4_4136 0.22 -1.1 4,579,167 + Pf1N1B4_4136 0.22 -0.5 4,579,167 + Pf1N1B4_4136 0.22 +0.2 4,579,167 + -1.1 4,579,168 - Pf1N1B4_4136 0.23 -0.9 4,579,168 - Pf1N1B4_4136 0.23 +1.5 4,579,168 - Pf1N1B4_4136 0.23 -1.1 4,579,570 - -0.3
Or see this region's nucleotide sequence