Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4048 and Pf1N1B4_4049 are separated by 59 nucleotides Pf1N1B4_4049 and Pf1N1B4_4050 are separated by 213 nucleotides Pf1N1B4_4050 and Pf1N1B4_4051 are separated by 38 nucleotides Pf1N1B4_4051 and Pf1N1B4_4052 are separated by 493 nucleotides
Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589
_4048
Pf1N1B4_4049 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 4,501,649 to 4,502,095
_4049
Pf1N1B4_4050 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,309 to 4,502,422
_4050
Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533
_4051
Pf1N1B4_4052 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,505,027 to 4,506,283
_4052
Position (kb)
4502
4503
4504
4505 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4501.709 kb on - strand at 4501.709 kb on - strand, within Pf1N1B4_4049 at 4501.806 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.855 kb on - strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4502.239 kb on + strand at 4502.239 kb on + strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.248 kb on - strand at 4502.248 kb on - strand at 4502.302 kb on + strand at 4502.310 kb on + strand at 4502.551 kb on - strand at 4502.663 kb on - strand at 4502.673 kb on + strand, within Pf1N1B4_4051 at 4502.674 kb on - strand, within Pf1N1B4_4051 at 4502.674 kb on - strand, within Pf1N1B4_4051 at 4502.678 kb on - strand, within Pf1N1B4_4051 at 4503.004 kb on - strand, within Pf1N1B4_4051 at 4503.020 kb on + strand, within Pf1N1B4_4051 at 4503.020 kb on + strand, within Pf1N1B4_4051 at 4503.389 kb on + strand, within Pf1N1B4_4051 at 4503.566 kb on + strand, within Pf1N1B4_4051 at 4503.625 kb on - strand, within Pf1N1B4_4051 at 4503.710 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.506 kb on + strand at 4504.506 kb on + strand at 4504.508 kb on + strand at 4504.508 kb on + strand at 4504.560 kb on + strand at 4504.692 kb on - strand at 4504.727 kb on + strand at 4504.769 kb on + strand at 4504.770 kb on - strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.780 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.856 kb on + strand at 4504.856 kb on + strand at 4504.910 kb on + strand at 4504.910 kb on + strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.953 kb on - strand at 4504.971 kb on + strand at 4504.972 kb on - strand at 4504.972 kb on - strand at 4504.981 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.140 kb on + strand at 4505.141 kb on - strand at 4505.153 kb on - strand, within Pf1N1B4_4052 at 4505.237 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand at 4505.320 kb on - strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,501,709 - +2.5 4,501,709 - Pf1N1B4_4049 0.13 -1.1 4,501,806 + Pf1N1B4_4049 0.35 +0.2 4,501,854 + Pf1N1B4_4049 0.46 +0.3 4,501,854 + Pf1N1B4_4049 0.46 -0.3 4,501,855 - Pf1N1B4_4049 0.46 +2.0 4,501,881 + Pf1N1B4_4049 0.52 +1.8 4,501,881 + Pf1N1B4_4049 0.52 +0.1 4,501,881 + Pf1N1B4_4049 0.52 +1.2 4,501,887 + -1.1 4,501,887 + Pf1N1B4_4049 0.53 -1.1 4,501,887 + Pf1N1B4_4049 0.53 -0.0 4,501,887 + Pf1N1B4_4049 0.53 +0.3 4,501,887 + Pf1N1B4_4049 0.53 -2.3 4,502,239 + +0.7 4,502,239 + -0.0 4,502,240 - -0.1 4,502,240 - -0.4 4,502,240 - +0.8 4,502,240 - +0.0 4,502,240 - +0.4 4,502,240 - +1.2 4,502,240 - +0.5 4,502,240 - -0.4 4,502,240 - -0.0 4,502,240 - +1.1 4,502,240 - -0.1 4,502,243 + -1.0 4,502,243 + +1.5 4,502,243 + -0.8 4,502,243 + -0.1 4,502,243 + +0.5 4,502,243 + +1.5 4,502,243 + +0.1 4,502,243 + +0.9 4,502,243 + -0.3 4,502,243 + +1.5 4,502,243 + -0.5 4,502,243 + +0.1 4,502,243 + +1.5 4,502,243 + -0.8 4,502,244 - -3.0 4,502,244 - +0.6 4,502,244 - -1.1 4,502,244 - +2.1 4,502,244 - +0.0 4,502,247 + +0.0 4,502,247 + +0.1 4,502,247 + -0.7 4,502,247 + +0.6 4,502,248 - -2.1 4,502,248 - +0.9 4,502,302 + +0.5 4,502,310 + -1.8 4,502,551 - -0.5 4,502,663 - -0.3 4,502,673 + Pf1N1B4_4051 0.10 -1.1 4,502,674 - Pf1N1B4_4051 0.10 -0.3 4,502,674 - Pf1N1B4_4051 0.10 -0.5 4,502,678 - Pf1N1B4_4051 0.10 -0.7 4,503,004 - Pf1N1B4_4051 0.26 -2.3 4,503,020 + Pf1N1B4_4051 0.27 -1.4 4,503,020 + Pf1N1B4_4051 0.27 -0.2 4,503,389 + Pf1N1B4_4051 0.45 +0.3 4,503,566 + Pf1N1B4_4051 0.53 -0.9 4,503,625 - Pf1N1B4_4051 0.56 -1.8 4,503,710 + Pf1N1B4_4051 0.60 -0.1 4,504,073 + Pf1N1B4_4051 0.78 +0.0 4,504,073 + Pf1N1B4_4051 0.78 +0.1 4,504,472 + -1.8 4,504,472 + -0.2 4,504,472 + +0.2 4,504,506 + -0.5 4,504,506 + +0.2 4,504,508 + +0.3 4,504,508 + +0.3 4,504,560 + +0.1 4,504,692 - -0.1 4,504,727 + -0.3 4,504,769 + +2.3 4,504,770 - +0.3 4,504,771 + -0.7 4,504,771 + -0.5 4,504,771 + +0.1 4,504,771 + -0.1 4,504,771 + +0.5 4,504,771 + +0.1 4,504,771 + +1.2 4,504,771 + -0.3 4,504,771 + +0.5 4,504,771 + -1.5 4,504,771 + +1.5 4,504,771 + -0.1 4,504,771 + -0.1 4,504,771 + -0.3 4,504,771 + +0.8 4,504,771 + -0.5 4,504,771 + -1.0 4,504,772 - +0.6 4,504,772 - +0.2 4,504,772 - +0.1 4,504,772 - -2.0 4,504,772 - -1.8 4,504,772 - +2.1 4,504,775 + -0.3 4,504,775 + -0.5 4,504,775 + -0.2 4,504,775 + -0.4 4,504,775 + -0.8 4,504,775 + +0.2 4,504,775 + +0.5 4,504,775 + -0.3 4,504,776 - +0.1 4,504,776 - -0.7 4,504,776 - +0.2 4,504,776 - -1.3 4,504,776 - +0.2 4,504,776 - -1.1 4,504,776 - -1.5 4,504,780 - -1.2 4,504,805 - +0.0 4,504,805 - +0.7 4,504,805 - -1.5 4,504,830 + -0.8 4,504,830 + -2.5 4,504,830 + -1.1 4,504,856 + -0.1 4,504,856 + -0.5 4,504,910 + -1.8 4,504,910 + +0.3 4,504,911 - +1.5 4,504,911 - -0.8 4,504,911 - -0.5 4,504,911 - +0.3 4,504,911 - -0.6 4,504,911 - +0.6 4,504,952 + -0.5 4,504,952 + -0.2 4,504,952 + +0.2 4,504,952 + -1.5 4,504,952 + +0.4 4,504,953 - -0.3 4,504,971 + +0.7 4,504,972 - +0.5 4,504,972 - -1.0 4,504,981 - +2.5 4,505,022 - -0.3 4,505,022 - -0.5 4,505,022 - -0.7 4,505,140 + -0.2 4,505,141 - -0.3 4,505,153 - Pf1N1B4_4052 0.10 +1.5 4,505,237 + Pf1N1B4_4052 0.17 +1.2 4,505,315 + Pf1N1B4_4052 0.23 -0.3 4,505,315 + Pf1N1B4_4052 0.23 +0.4 4,505,315 + Pf1N1B4_4052 0.23 -0.2 4,505,315 + Pf1N1B4_4052 0.23 +0.2 4,505,316 - Pf1N1B4_4052 0.23 +1.2 4,505,316 - Pf1N1B4_4052 0.23 -0.4 4,505,319 + Pf1N1B4_4052 0.23 +0.5 4,505,319 + Pf1N1B4_4052 0.23 +0.1 4,505,319 + -0.5 4,505,320 - Pf1N1B4_4052 0.23 +0.0 4,505,405 + Pf1N1B4_4052 0.30 -0.7 4,505,405 + Pf1N1B4_4052 0.30 +0.9 4,505,405 + Pf1N1B4_4052 0.30 -0.1 4,505,405 + Pf1N1B4_4052 0.30 -0.2 4,505,405 + Pf1N1B4_4052 0.30 +0.6 4,505,405 + +0.5 4,505,406 - Pf1N1B4_4052 0.30 -0.7 4,505,406 - Pf1N1B4_4052 0.30 -1.8 4,505,510 + Pf1N1B4_4052 0.38 -0.1 4,505,510 + Pf1N1B4_4052 0.38 -2.1 4,505,510 + Pf1N1B4_4052 0.38 +0.5 4,505,511 - -0.1
Or see this region's nucleotide sequence