Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3985

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3984 and Pf1N1B4_3985 overlap by 1 nucleotidesPf1N1B4_3985 and Pf1N1B4_3986 are separated by 115 nucleotides Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data), at 4,424,874 to 4,426,829 _3984 Pf1N1B4_3985 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 4,426,829 to 4,427,644 _3985 Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367 _3986 Position (kb) 4426 4427 4428Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4426.015 kb on + strand, within Pf1N1B4_3984at 4426.150 kb on + strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.152 kb on + strand, within Pf1N1B4_3984at 4426.153 kb on - strand, within Pf1N1B4_3984at 4426.153 kb on - strand, within Pf1N1B4_3984at 4426.213 kb on + strand, within Pf1N1B4_3984at 4426.214 kb on - strand, within Pf1N1B4_3984at 4426.639 kb on + strandat 4426.640 kb on - strandat 4426.640 kb on - strandat 4427.321 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.891 kb on - strandat 4427.891 kb on - strandat 4427.891 kb on - strandat 4427.891 kb on - strandat 4427.894 kb on - strandat 4428.104 kb on - strand, within Pf1N1B4_3986at 4428.215 kb on - strand, within Pf1N1B4_3986at 4428.392 kb on + strand, within Pf1N1B4_3986

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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4,426,015 + Pf1N1B4_3984 0.58 +0.5
4,426,150 + Pf1N1B4_3984 0.65 -1.8
4,426,151 - Pf1N1B4_3984 0.65 -1.1
4,426,151 - Pf1N1B4_3984 0.65 +0.5
4,426,151 - Pf1N1B4_3984 0.65 +0.5
4,426,151 - Pf1N1B4_3984 0.65 +0.5
4,426,151 - Pf1N1B4_3984 0.65 +0.9
4,426,152 + Pf1N1B4_3984 0.65 -0.2
4,426,153 - Pf1N1B4_3984 0.65 +0.5
4,426,153 - Pf1N1B4_3984 0.65 -0.1
4,426,213 + Pf1N1B4_3984 0.68 -0.3
4,426,214 - Pf1N1B4_3984 0.69 +2.1
4,426,639 + -0.8
4,426,640 - +0.9
4,426,640 - +0.7
4,427,321 + Pf1N1B4_3985 0.60 -0.7
4,427,361 + Pf1N1B4_3985 0.65 -2.8
4,427,361 + Pf1N1B4_3985 0.65 +0.2
4,427,361 + Pf1N1B4_3985 0.65 -1.1
4,427,891 - -0.9
4,427,891 - -3.1
4,427,891 - -0.3
4,427,891 - -0.3
4,427,894 - +0.8
4,428,104 - Pf1N1B4_3986 0.21 +0.0
4,428,215 - Pf1N1B4_3986 0.28 -1.3
4,428,392 + Pf1N1B4_3986 0.39 -0.9

Or see this region's nucleotide sequence