Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3976 and Pf1N1B4_3977 overlap by 4 nucleotides Pf1N1B4_3977 and Pf1N1B4_3978 are separated by 155 nucleotides Pf1N1B4_3978 and Pf1N1B4_3979 are separated by 478 nucleotides
Pf1N1B4_3976 - Transmembrane regulator protein PrtR, at 4,417,174 to 4,417,926
_3976
Pf1N1B4_3977 - RNA polymerase sigma-54 factor RpoN, at 4,417,923 to 4,418,483
_3977
Pf1N1B4_3978 - Catalase (EC 1.11.1.6), at 4,418,639 to 4,419,688
_3978
Pf1N1B4_3979 - MFS permease protein, at 4,420,167 to 4,421,459
_3979
Position (kb)
4418
4419
4420 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.887 kb on - strand at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.052 kb on - strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.054 kb on - strand, within Pf1N1B4_3977 at 4418.441 kb on + strand at 4418.449 kb on - strand at 4418.529 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.599 kb on - strand at 4418.714 kb on + strand at 4418.791 kb on - strand, within Pf1N1B4_3978 at 4418.809 kb on + strand, within Pf1N1B4_3978 at 4418.993 kb on - strand, within Pf1N1B4_3978 at 4419.071 kb on + strand at 4419.072 kb on - strand, within Pf1N1B4_3978 at 4419.072 kb on - strand, within Pf1N1B4_3978 at 4419.224 kb on + strand, within Pf1N1B4_3978 at 4419.224 kb on + strand, within Pf1N1B4_3978 at 4419.225 kb on - strand, within Pf1N1B4_3978 at 4419.225 kb on - strand, within Pf1N1B4_3978 at 4419.225 kb on - strand, within Pf1N1B4_3978 at 4419.406 kb on + strand, within Pf1N1B4_3978 at 4419.406 kb on + strand, within Pf1N1B4_3978 at 4419.407 kb on - strand at 4419.407 kb on - strand, within Pf1N1B4_3978 at 4419.954 kb on + strand at 4419.955 kb on - strand at 4419.964 kb on + strand at 4419.964 kb on + strand at 4419.965 kb on - strand at 4420.035 kb on + strand at 4420.035 kb on + strand at 4420.035 kb on + strand at 4420.035 kb on + strand at 4420.035 kb on + strand at 4420.038 kb on + strand at 4420.038 kb on + strand at 4420.038 kb on + strand at 4420.038 kb on + strand at 4420.039 kb on - strand at 4420.121 kb on - strand at 4420.121 kb on - strand at 4420.121 kb on - strand at 4420.315 kb on + strand, within Pf1N1B4_3979 at 4420.382 kb on - strand, within Pf1N1B4_3979 at 4420.382 kb on - strand, within Pf1N1B4_3979 at 4420.382 kb on - strand, within Pf1N1B4_3979 at 4420.427 kb on - strand, within Pf1N1B4_3979 at 4420.438 kb on + strand, within Pf1N1B4_3979 at 4420.480 kb on + strand, within Pf1N1B4_3979 at 4420.480 kb on + strand, within Pf1N1B4_3979 at 4420.480 kb on + strand, within Pf1N1B4_3979 at 4420.481 kb on - strand, within Pf1N1B4_3979 at 4420.481 kb on - strand, within Pf1N1B4_3979 at 4420.481 kb on - strand, within Pf1N1B4_3979 at 4420.533 kb on - strand, within Pf1N1B4_3979
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,417,838 + Pf1N1B4_3976 0.88 -1.5 4,417,838 + Pf1N1B4_3976 0.88 +0.2 4,417,839 - Pf1N1B4_3976 0.88 -0.7 4,417,839 - Pf1N1B4_3976 0.88 +0.3 4,417,839 - Pf1N1B4_3976 0.88 -0.3 4,417,839 - Pf1N1B4_3976 0.88 -0.5 4,417,839 - Pf1N1B4_3976 0.88 -0.1 4,417,887 - -1.9 4,418,051 + Pf1N1B4_3977 0.23 -1.4 4,418,051 + Pf1N1B4_3977 0.23 +0.8 4,418,051 + Pf1N1B4_3977 0.23 +0.2 4,418,051 + Pf1N1B4_3977 0.23 -1.1 4,418,051 + Pf1N1B4_3977 0.23 -1.1 4,418,052 - Pf1N1B4_3977 0.23 -1.1 4,418,053 + Pf1N1B4_3977 0.23 +0.5 4,418,053 + -0.1 4,418,053 + Pf1N1B4_3977 0.23 +0.1 4,418,053 + Pf1N1B4_3977 0.23 +0.5 4,418,054 - Pf1N1B4_3977 0.23 -0.5 4,418,441 + -0.3 4,418,449 - -1.3 4,418,529 + +0.8 4,418,598 + +0.3 4,418,598 + -0.5 4,418,598 + -1.1 4,418,598 + -1.3 4,418,599 - +3.7 4,418,714 + +1.1 4,418,791 - Pf1N1B4_3978 0.14 +0.3 4,418,809 + Pf1N1B4_3978 0.16 -0.5 4,418,993 - Pf1N1B4_3978 0.34 -0.8 4,419,071 + +0.5 4,419,072 - Pf1N1B4_3978 0.41 +0.5 4,419,072 - Pf1N1B4_3978 0.41 -0.8 4,419,224 + Pf1N1B4_3978 0.56 -0.1 4,419,224 + Pf1N1B4_3978 0.56 +0.2 4,419,225 - Pf1N1B4_3978 0.56 -0.5 4,419,225 - Pf1N1B4_3978 0.56 -2.6 4,419,225 - Pf1N1B4_3978 0.56 -2.3 4,419,406 + Pf1N1B4_3978 0.73 -0.1 4,419,406 + Pf1N1B4_3978 0.73 +0.5 4,419,407 - +1.1 4,419,407 - Pf1N1B4_3978 0.73 +0.2 4,419,954 + +0.9 4,419,955 - +0.5 4,419,964 + +2.1 4,419,964 + -0.5 4,419,965 - +0.9 4,420,035 + +1.1 4,420,035 + +0.1 4,420,035 + -0.5 4,420,035 + +2.3 4,420,035 + -0.5 4,420,038 + -0.1 4,420,038 + +1.8 4,420,038 + +1.5 4,420,038 + +0.3 4,420,039 - +0.2 4,420,121 - -0.1 4,420,121 - -2.5 4,420,121 - -0.5 4,420,315 + Pf1N1B4_3979 0.11 +1.2 4,420,382 - Pf1N1B4_3979 0.17 +0.5 4,420,382 - Pf1N1B4_3979 0.17 -0.1 4,420,382 - Pf1N1B4_3979 0.17 -0.7 4,420,427 - Pf1N1B4_3979 0.20 -0.1 4,420,438 + Pf1N1B4_3979 0.21 -0.4 4,420,480 + Pf1N1B4_3979 0.24 -1.4 4,420,480 + Pf1N1B4_3979 0.24 +0.3 4,420,480 + Pf1N1B4_3979 0.24 -1.1 4,420,481 - Pf1N1B4_3979 0.24 -0.2 4,420,481 - Pf1N1B4_3979 0.24 +0.9 4,420,481 - Pf1N1B4_3979 0.24 +3.3 4,420,533 - Pf1N1B4_3979 0.28 -1.5
Or see this region's nucleotide sequence