Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3974 and Pf1N1B4_3975 are separated by 244 nucleotides Pf1N1B4_3975 and Pf1N1B4_3976 are separated by 38 nucleotides Pf1N1B4_3976 and Pf1N1B4_3977 overlap by 4 nucleotides
Pf1N1B4_3974 - ABC transporter, ATP-binding protein, at 4,415,029 to 4,416,054
_3974
Pf1N1B4_3975 - Transcriptional regulator, IclR family, at 4,416,299 to 4,417,135
_3975
Pf1N1B4_3976 - Transmembrane regulator protein PrtR, at 4,417,174 to 4,417,926
_3976
Pf1N1B4_3977 - RNA polymerase sigma-54 factor RpoN, at 4,417,923 to 4,418,483
_3977
Position (kb)
4416
4417
4418 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4415.725 kb on + strand at 4415.726 kb on - strand, within Pf1N1B4_3974 at 4415.746 kb on - strand at 4416.078 kb on + strand at 4416.116 kb on + strand at 4416.117 kb on - strand at 4416.214 kb on + strand at 4416.233 kb on + strand at 4416.254 kb on - strand at 4416.812 kb on + strand at 4416.812 kb on + strand, within Pf1N1B4_3975 at 4416.845 kb on + strand, within Pf1N1B4_3975 at 4416.918 kb on + strand, within Pf1N1B4_3975 at 4416.919 kb on - strand, within Pf1N1B4_3975 at 4416.919 kb on - strand, within Pf1N1B4_3975 at 4417.238 kb on + strand at 4417.238 kb on + strand at 4417.238 kb on + strand at 4417.239 kb on - strand at 4417.239 kb on - strand at 4417.262 kb on + strand, within Pf1N1B4_3976 at 4417.318 kb on - strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand at 4417.338 kb on - strand at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.370 kb on + strand at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.409 kb on - strand, within Pf1N1B4_3976 at 4417.498 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.887 kb on - strand at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.052 kb on - strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.054 kb on - strand, within Pf1N1B4_3977
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,415,725 + +2.1 4,415,726 - Pf1N1B4_3974 0.68 -1.1 4,415,746 - +0.9 4,416,078 + +0.5 4,416,116 + -0.2 4,416,117 - +0.0 4,416,214 + +0.8 4,416,233 + +0.3 4,416,254 - +0.5 4,416,812 + -0.7 4,416,812 + Pf1N1B4_3975 0.61 +0.4 4,416,845 + Pf1N1B4_3975 0.65 +1.3 4,416,918 + Pf1N1B4_3975 0.74 +1.3 4,416,919 - Pf1N1B4_3975 0.74 +0.0 4,416,919 - Pf1N1B4_3975 0.74 +1.4 4,417,238 + +1.3 4,417,238 + -0.5 4,417,238 + +0.5 4,417,239 - -2.1 4,417,239 - +0.5 4,417,262 + Pf1N1B4_3976 0.12 -0.5 4,417,318 - Pf1N1B4_3976 0.19 +0.1 4,417,337 + Pf1N1B4_3976 0.22 +0.5 4,417,337 + Pf1N1B4_3976 0.22 -0.5 4,417,337 + Pf1N1B4_3976 0.22 -0.5 4,417,337 + Pf1N1B4_3976 0.22 -0.1 4,417,337 + +0.5 4,417,338 - +1.5 4,417,370 + Pf1N1B4_3976 0.26 +1.3 4,417,370 + Pf1N1B4_3976 0.26 -0.5 4,417,370 + +0.8 4,417,370 + Pf1N1B4_3976 0.26 +0.9 4,417,371 - Pf1N1B4_3976 0.26 -1.8 4,417,371 - -0.1 4,417,371 - Pf1N1B4_3976 0.26 +0.4 4,417,371 - Pf1N1B4_3976 0.26 +1.2 4,417,371 - Pf1N1B4_3976 0.26 -0.1 4,417,371 - Pf1N1B4_3976 0.26 +1.3 4,417,371 - Pf1N1B4_3976 0.26 -0.2 4,417,371 - Pf1N1B4_3976 0.26 -2.6 4,417,371 - Pf1N1B4_3976 0.26 +1.3 4,417,409 - Pf1N1B4_3976 0.31 +0.8 4,417,498 - Pf1N1B4_3976 0.43 +1.3 4,417,521 - Pf1N1B4_3976 0.46 +1.1 4,417,521 - Pf1N1B4_3976 0.46 -0.5 4,417,521 - Pf1N1B4_3976 0.46 -1.1 4,417,838 + Pf1N1B4_3976 0.88 -1.5 4,417,838 + Pf1N1B4_3976 0.88 +0.2 4,417,839 - Pf1N1B4_3976 0.88 -0.7 4,417,839 - Pf1N1B4_3976 0.88 +0.3 4,417,839 - Pf1N1B4_3976 0.88 -0.3 4,417,839 - Pf1N1B4_3976 0.88 -0.5 4,417,839 - Pf1N1B4_3976 0.88 -0.1 4,417,887 - -1.9 4,418,051 + Pf1N1B4_3977 0.23 -1.4 4,418,051 + Pf1N1B4_3977 0.23 +0.8 4,418,051 + Pf1N1B4_3977 0.23 +0.2 4,418,051 + Pf1N1B4_3977 0.23 -1.1 4,418,051 + Pf1N1B4_3977 0.23 -1.1 4,418,052 - Pf1N1B4_3977 0.23 -1.1 4,418,053 + Pf1N1B4_3977 0.23 +0.5 4,418,053 + -0.1 4,418,053 + Pf1N1B4_3977 0.23 +0.1 4,418,053 + Pf1N1B4_3977 0.23 +0.5 4,418,054 - Pf1N1B4_3977 0.23 -0.5
Or see this region's nucleotide sequence