Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3890 and Pf1N1B4_3891 are separated by 185 nucleotides Pf1N1B4_3891 and Pf1N1B4_3892 are separated by 203 nucleotides Pf1N1B4_3892 and Pf1N1B4_3893 are separated by 349 nucleotides
Pf1N1B4_3890 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 4,319,982 to 4,321,055
_3890
Pf1N1B4_3891 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 4,321,241 to 4,321,492
_3891
Pf1N1B4_3892 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 4,321,696 to 4,323,096
_3892
Pf1N1B4_3893 - Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290), at 4,323,446 to 4,324,588
_3893
Position (kb)
4321
4322
4323
4324 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.929 kb on + strand, within Pf1N1B4_3890 at 4320.987 kb on + strand at 4320.987 kb on + strand at 4321.033 kb on + strand at 4321.192 kb on + strand at 4321.193 kb on - strand at 4321.193 kb on - strand at 4321.235 kb on + strand at 4321.490 kb on + strand at 4321.504 kb on + strand at 4321.504 kb on + strand at 4321.505 kb on - strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.511 kb on + strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.512 kb on - strand at 4321.513 kb on + strand at 4321.513 kb on + strand at 4321.513 kb on + strand at 4321.514 kb on - strand at 4321.514 kb on - strand at 4321.533 kb on + strand at 4321.534 kb on - strand at 4321.534 kb on - strand at 4321.534 kb on - strand at 4321.649 kb on + strand at 4321.649 kb on + strand at 4321.664 kb on - strand at 4321.664 kb on - strand at 4321.673 kb on + strand at 4321.673 kb on + strand at 4321.673 kb on + strand at 4321.674 kb on - strand at 4321.784 kb on - strand at 4321.865 kb on - strand, within Pf1N1B4_3892 at 4321.986 kb on - strand, within Pf1N1B4_3892 at 4322.034 kb on - strand, within Pf1N1B4_3892 at 4322.063 kb on - strand, within Pf1N1B4_3892 at 4322.090 kb on + strand, within Pf1N1B4_3892 at 4322.091 kb on - strand, within Pf1N1B4_3892 at 4322.537 kb on + strand, within Pf1N1B4_3892 at 4322.537 kb on + strand at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand, within Pf1N1B4_3892 at 4322.538 kb on - strand at 4322.550 kb on + strand, within Pf1N1B4_3892 at 4322.551 kb on - strand, within Pf1N1B4_3892 at 4322.551 kb on - strand, within Pf1N1B4_3892 at 4322.633 kb on + strand, within Pf1N1B4_3892 at 4322.675 kb on + strand, within Pf1N1B4_3892 at 4322.675 kb on + strand, within Pf1N1B4_3892 at 4322.676 kb on - strand, within Pf1N1B4_3892 at 4322.676 kb on - strand, within Pf1N1B4_3892 at 4322.676 kb on - strand, within Pf1N1B4_3892 at 4323.149 kb on + strand at 4323.150 kb on - strand at 4323.195 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.410 kb on + strand at 4323.411 kb on - strand at 4323.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,320,900 + Pf1N1B4_3890 0.85 -1.2 4,320,900 + Pf1N1B4_3890 0.85 -0.4 4,320,900 + Pf1N1B4_3890 0.85 +0.2 4,320,929 + Pf1N1B4_3890 0.88 +0.6 4,320,987 + -0.0 4,320,987 + -1.0 4,321,033 + +0.1 4,321,192 + +2.0 4,321,193 - -0.8 4,321,193 - +1.0 4,321,235 + +0.6 4,321,490 + +0.9 4,321,504 + -2.0 4,321,504 + -0.4 4,321,505 - -0.0 4,321,511 + +0.4 4,321,511 + -1.6 4,321,511 + +1.1 4,321,511 + -0.4 4,321,511 + +1.0 4,321,511 + +0.3 4,321,511 + +0.8 4,321,511 + -1.2 4,321,511 + -1.3 4,321,511 + -0.4 4,321,511 + +0.6 4,321,511 + -0.1 4,321,511 + +0.9 4,321,511 + +0.7 4,321,512 - +1.3 4,321,512 - -0.7 4,321,512 - -1.0 4,321,512 - +0.6 4,321,512 - +0.2 4,321,512 - +1.6 4,321,512 - +1.4 4,321,512 - +1.6 4,321,512 - +1.0 4,321,512 - -0.3 4,321,512 - +0.6 4,321,513 + -0.2 4,321,513 + +1.3 4,321,513 + -0.6 4,321,514 - -0.8 4,321,514 - +0.6 4,321,533 + -0.7 4,321,534 - -0.9 4,321,534 - -0.4 4,321,534 - +1.6 4,321,649 + -1.3 4,321,649 + -0.2 4,321,664 - +0.6 4,321,664 - -2.2 4,321,673 + +0.6 4,321,673 + +1.3 4,321,673 + +0.6 4,321,674 - -0.0 4,321,784 - -2.0 4,321,865 - Pf1N1B4_3892 0.12 -0.8 4,321,986 - Pf1N1B4_3892 0.21 -0.0 4,322,034 - Pf1N1B4_3892 0.24 +1.3 4,322,063 - Pf1N1B4_3892 0.26 -1.8 4,322,090 + Pf1N1B4_3892 0.28 -0.4 4,322,091 - Pf1N1B4_3892 0.28 +0.9 4,322,537 + Pf1N1B4_3892 0.60 +0.8 4,322,537 + +1.1 4,322,538 - Pf1N1B4_3892 0.60 -1.8 4,322,538 - Pf1N1B4_3892 0.60 -0.5 4,322,538 - Pf1N1B4_3892 0.60 -0.9 4,322,538 - Pf1N1B4_3892 0.60 -1.8 4,322,538 - +1.6 4,322,550 + Pf1N1B4_3892 0.61 +0.6 4,322,551 - Pf1N1B4_3892 0.61 +0.6 4,322,551 - Pf1N1B4_3892 0.61 -1.2 4,322,633 + Pf1N1B4_3892 0.67 -0.7 4,322,675 + Pf1N1B4_3892 0.70 -1.4 4,322,675 + Pf1N1B4_3892 0.70 +0.0 4,322,676 - Pf1N1B4_3892 0.70 -1.0 4,322,676 - Pf1N1B4_3892 0.70 -1.2 4,322,676 - Pf1N1B4_3892 0.70 -0.6 4,323,149 + +0.6 4,323,150 - +0.6 4,323,195 + -0.1 4,323,410 + +0.1 4,323,410 + +0.3 4,323,410 + -1.4 4,323,410 + -0.6 4,323,410 + -0.3 4,323,410 + -1.0 4,323,411 - +0.1 4,323,411 - -0.0
Or see this region's nucleotide sequence