Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3784 and Pf1N1B4_3785 are separated by 96 nucleotides Pf1N1B4_3785 and Pf1N1B4_3786 are separated by 153 nucleotides Pf1N1B4_3786 and Pf1N1B4_3787 are separated by 83 nucleotides
Pf1N1B4_3784 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,193,040 to 4,194,878
_3784
Pf1N1B4_3785 - FOG: Ankyrin repeat, at 4,194,975 to 4,195,493
_3785
Pf1N1B4_3786 - FIG00955145: hypothetical protein, at 4,195,647 to 4,196,177
_3786
Pf1N1B4_3787 - FIG00960829: hypothetical protein, at 4,196,261 to 4,196,452
_3787
Position (kb)
4194
4195
4196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4194.101 kb on + strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.114 kb on + strand, within Pf1N1B4_3784 at 4194.207 kb on + strand, within Pf1N1B4_3784 at 4194.208 kb on - strand, within Pf1N1B4_3784 at 4194.239 kb on + strand, within Pf1N1B4_3784 at 4194.239 kb on + strand, within Pf1N1B4_3784 at 4194.239 kb on + strand, within Pf1N1B4_3784 at 4194.387 kb on + strand, within Pf1N1B4_3784 at 4194.388 kb on - strand, within Pf1N1B4_3784 at 4194.388 kb on - strand, within Pf1N1B4_3784 at 4194.463 kb on - strand, within Pf1N1B4_3784 at 4194.546 kb on + strand, within Pf1N1B4_3784 at 4194.546 kb on + strand, within Pf1N1B4_3784 at 4194.547 kb on - strand, within Pf1N1B4_3784 at 4194.669 kb on + strand, within Pf1N1B4_3784 at 4194.670 kb on - strand, within Pf1N1B4_3784 at 4194.792 kb on + strand at 4194.819 kb on + strand at 4194.819 kb on + strand at 4194.819 kb on + strand at 4194.819 kb on + strand at 4194.820 kb on - strand at 4194.820 kb on - strand at 4194.889 kb on - strand at 4194.940 kb on - strand at 4195.100 kb on + strand, within Pf1N1B4_3785 at 4195.100 kb on + strand, within Pf1N1B4_3785 at 4195.101 kb on - strand, within Pf1N1B4_3785 at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.789 kb on - strand, within Pf1N1B4_3786 at 4195.872 kb on + strand at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4196.022 kb on + strand, within Pf1N1B4_3786 at 4196.185 kb on + strand at 4196.193 kb on + strand at 4196.194 kb on - strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.229 kb on - strand at 4196.238 kb on + strand at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,194,101 + Pf1N1B4_3784 0.58 -1.4 4,194,102 - Pf1N1B4_3784 0.58 -0.7 4,194,102 - Pf1N1B4_3784 0.58 +0.2 4,194,102 - Pf1N1B4_3784 0.58 +0.8 4,194,102 - Pf1N1B4_3784 0.58 +0.6 4,194,102 - Pf1N1B4_3784 0.58 +0.6 4,194,114 + Pf1N1B4_3784 0.58 +0.6 4,194,207 + Pf1N1B4_3784 0.63 +1.1 4,194,208 - Pf1N1B4_3784 0.64 -1.2 4,194,239 + Pf1N1B4_3784 0.65 +0.9 4,194,239 + Pf1N1B4_3784 0.65 -0.7 4,194,239 + Pf1N1B4_3784 0.65 +0.6 4,194,387 + Pf1N1B4_3784 0.73 -0.4 4,194,388 - Pf1N1B4_3784 0.73 -0.2 4,194,388 - Pf1N1B4_3784 0.73 +1.6 4,194,463 - Pf1N1B4_3784 0.77 +0.1 4,194,546 + Pf1N1B4_3784 0.82 +2.8 4,194,546 + Pf1N1B4_3784 0.82 +0.3 4,194,547 - Pf1N1B4_3784 0.82 +0.6 4,194,669 + Pf1N1B4_3784 0.89 -0.4 4,194,670 - Pf1N1B4_3784 0.89 -1.3 4,194,792 + -1.0 4,194,819 + +1.0 4,194,819 + +0.0 4,194,819 + -0.4 4,194,819 + +1.1 4,194,820 - -1.0 4,194,820 - -0.2 4,194,889 - -1.4 4,194,940 - -0.4 4,195,100 + Pf1N1B4_3785 0.24 -1.2 4,195,100 + Pf1N1B4_3785 0.24 -0.4 4,195,101 - Pf1N1B4_3785 0.24 +0.3 4,195,590 - -1.4 4,195,590 - -0.2 4,195,590 - -0.7 4,195,590 - -0.7 4,195,590 - +1.2 4,195,595 + +0.6 4,195,595 + -0.9 4,195,595 + +0.7 4,195,595 + -0.4 4,195,595 + -0.4 4,195,595 + +0.2 4,195,596 - +0.0 4,195,596 - +0.6 4,195,596 - +1.3 4,195,596 - +0.6 4,195,596 - +0.3 4,195,596 - +0.1 4,195,596 - +1.9 4,195,596 - -0.0 4,195,788 + Pf1N1B4_3786 0.27 -2.1 4,195,788 + Pf1N1B4_3786 0.27 +1.6 4,195,788 + Pf1N1B4_3786 0.27 +0.0 4,195,789 - Pf1N1B4_3786 0.27 -0.2 4,195,872 + +1.6 4,195,872 + Pf1N1B4_3786 0.42 +0.2 4,195,872 + Pf1N1B4_3786 0.42 -0.4 4,195,872 + Pf1N1B4_3786 0.42 +0.3 4,195,873 - Pf1N1B4_3786 0.43 +0.5 4,195,873 - Pf1N1B4_3786 0.43 +0.4 4,195,873 - Pf1N1B4_3786 0.43 -0.1 4,195,873 - Pf1N1B4_3786 0.43 +1.3 4,195,873 - Pf1N1B4_3786 0.43 -0.1 4,195,873 - Pf1N1B4_3786 0.43 +0.2 4,195,873 - Pf1N1B4_3786 0.43 +0.4 4,196,022 + Pf1N1B4_3786 0.71 -2.8 4,196,185 + -0.6 4,196,193 + +1.6 4,196,194 - -1.4 4,196,228 + +0.4 4,196,228 + -0.5 4,196,228 + -1.1 4,196,228 + +0.5 4,196,228 + -1.7 4,196,228 + +0.6 4,196,228 + +0.9 4,196,229 - -0.3 4,196,238 + +0.3 4,196,335 + Pf1N1B4_3787 0.39 -0.4 4,196,335 + Pf1N1B4_3787 0.39 +0.4 4,196,336 - Pf1N1B4_3787 0.39 +0.6 4,196,336 - Pf1N1B4_3787 0.39 +0.2 4,196,336 - Pf1N1B4_3787 0.39 +0.2 4,196,336 - Pf1N1B4_3787 0.39 -0.7 4,196,377 - Pf1N1B4_3787 0.60 -1.0 4,196,377 - Pf1N1B4_3787 0.60 +0.6
Or see this region's nucleotide sequence