Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5225 and Pf1N1B4_5226 are separated by 21 nucleotides Pf1N1B4_5226 and Pf1N1B4_5227 are separated by 190 nucleotides Pf1N1B4_5227 and Pf1N1B4_5228 are separated by 22 nucleotides Pf1N1B4_5228 and Pf1N1B4_3732 are separated by 315 nucleotides Pf1N1B4_3732 and Pf1N1B4_3733 are separated by 3 nucleotides
Pf1N1B4_5225 - tRNA-Val-TAC, at 4,141,941 to 4,142,013
_5225
Pf1N1B4_5226 - tRNA-Asp-GTC, at 4,142,035 to 4,142,108
_5226
Pf1N1B4_5227 - tRNA-Val-TAC, at 4,142,299 to 4,142,371
_5227
Pf1N1B4_5228 - tRNA-Asp-GTC, at 4,142,394 to 4,142,467
_5228
Pf1N1B4_3732 - Catalase (EC 1.11.1.6), at 4,142,783 to 4,143,919
_3732
Pf1N1B4_3733 - FIG00959155: hypothetical protein, at 4,143,923 to 4,145,755
_3733
Position (kb)
4142
4143
4144 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4141.800 kb on + strand at 4141.800 kb on + strand at 4141.801 kb on - strand at 4141.815 kb on + strand at 4141.816 kb on - strand at 4141.835 kb on - strand at 4141.850 kb on + strand at 4142.377 kb on + strand at 4142.377 kb on + strand at 4142.471 kb on + strand at 4142.626 kb on - strand at 4142.665 kb on + strand at 4142.752 kb on + strand at 4142.752 kb on + strand at 4142.752 kb on + strand at 4142.771 kb on + strand at 4142.771 kb on + strand at 4142.772 kb on - strand at 4142.772 kb on - strand at 4142.772 kb on - strand at 4142.772 kb on - strand at 4142.829 kb on + strand at 4142.829 kb on + strand at 4142.829 kb on + strand at 4142.829 kb on + strand at 4142.829 kb on + strand at 4142.830 kb on - strand at 4142.830 kb on - strand at 4142.830 kb on - strand at 4142.830 kb on - strand at 4142.830 kb on - strand at 4142.830 kb on - strand at 4142.870 kb on - strand at 4143.030 kb on + strand at 4143.030 kb on + strand, within Pf1N1B4_3732 at 4143.030 kb on + strand, within Pf1N1B4_3732 at 4143.030 kb on + strand, within Pf1N1B4_3732 at 4143.030 kb on + strand, within Pf1N1B4_3732 at 4143.030 kb on + strand, within Pf1N1B4_3732 at 4143.031 kb on - strand at 4143.031 kb on - strand, within Pf1N1B4_3732 at 4143.031 kb on - strand, within Pf1N1B4_3732 at 4143.031 kb on - strand, within Pf1N1B4_3732 at 4143.031 kb on - strand, within Pf1N1B4_3732 at 4143.031 kb on - strand, within Pf1N1B4_3732 at 4143.059 kb on + strand, within Pf1N1B4_3732 at 4143.060 kb on - strand at 4143.196 kb on + strand, within Pf1N1B4_3732 at 4143.202 kb on + strand, within Pf1N1B4_3732 at 4143.237 kb on + strand, within Pf1N1B4_3732 at 4143.237 kb on + strand, within Pf1N1B4_3732 at 4143.384 kb on + strand, within Pf1N1B4_3732 at 4143.491 kb on + strand, within Pf1N1B4_3732 at 4143.491 kb on + strand, within Pf1N1B4_3732 at 4143.611 kb on + strand, within Pf1N1B4_3732 at 4143.611 kb on + strand, within Pf1N1B4_3732 at 4143.612 kb on - strand, within Pf1N1B4_3732 at 4143.721 kb on + strand, within Pf1N1B4_3732 at 4143.722 kb on - strand at 4143.725 kb on + strand, within Pf1N1B4_3732 at 4143.725 kb on + strand, within Pf1N1B4_3732 at 4143.726 kb on - strand at 4144.153 kb on + strand, within Pf1N1B4_3733 at 4144.154 kb on - strand, within Pf1N1B4_3733 at 4144.161 kb on + strand, within Pf1N1B4_3733 at 4144.179 kb on + strand, within Pf1N1B4_3733 at 4144.179 kb on + strand, within Pf1N1B4_3733 at 4144.179 kb on + strand, within Pf1N1B4_3733 at 4144.179 kb on + strand, within Pf1N1B4_3733 at 4144.180 kb on - strand, within Pf1N1B4_3733 at 4144.180 kb on - strand, within Pf1N1B4_3733 at 4144.180 kb on - strand, within Pf1N1B4_3733 at 4144.293 kb on + strand, within Pf1N1B4_3733 at 4144.293 kb on + strand, within Pf1N1B4_3733 at 4144.293 kb on + strand at 4144.294 kb on - strand, within Pf1N1B4_3733 at 4144.297 kb on + strand, within Pf1N1B4_3733 at 4144.297 kb on + strand, within Pf1N1B4_3733 at 4144.297 kb on + strand, within Pf1N1B4_3733 at 4144.407 kb on + strand, within Pf1N1B4_3733 at 4144.407 kb on + strand, within Pf1N1B4_3733 at 4144.408 kb on - strand, within Pf1N1B4_3733 at 4144.441 kb on + strand, within Pf1N1B4_3733 at 4144.475 kb on + strand, within Pf1N1B4_3733 at 4144.475 kb on + strand, within Pf1N1B4_3733 at 4144.475 kb on + strand, within Pf1N1B4_3733 at 4144.476 kb on - strand, within Pf1N1B4_3733 at 4144.476 kb on - strand, within Pf1N1B4_3733 at 4144.476 kb on - strand, within Pf1N1B4_3733 at 4144.476 kb on - strand, within Pf1N1B4_3733 at 4144.476 kb on - strand, within Pf1N1B4_3733 at 4144.476 kb on - strand, within Pf1N1B4_3733
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 4,141,800 + -1.6 4,141,800 + +1.8 4,141,801 - +0.6 4,141,815 + -0.8 4,141,816 - -1.0 4,141,835 - +0.0 4,141,850 + -0.1 4,142,377 + +2.2 4,142,377 + +1.0 4,142,471 + +0.5 4,142,626 - -0.4 4,142,665 + -1.0 4,142,752 + -0.6 4,142,752 + -2.0 4,142,752 + +1.2 4,142,771 + +2.2 4,142,771 + -0.5 4,142,772 - +1.2 4,142,772 - +1.6 4,142,772 - -0.4 4,142,772 - +0.3 4,142,829 + -0.5 4,142,829 + -0.7 4,142,829 + -0.0 4,142,829 + +0.9 4,142,829 + +0.2 4,142,830 - +0.5 4,142,830 - -0.0 4,142,830 - -0.4 4,142,830 - -0.3 4,142,830 - +2.0 4,142,830 - -0.6 4,142,870 - -0.4 4,143,030 + +0.0 4,143,030 + Pf1N1B4_3732 0.22 +0.3 4,143,030 + Pf1N1B4_3732 0.22 -0.4 4,143,030 + Pf1N1B4_3732 0.22 +0.9 4,143,030 + Pf1N1B4_3732 0.22 -2.5 4,143,030 + Pf1N1B4_3732 0.22 +0.2 4,143,031 - -0.7 4,143,031 - Pf1N1B4_3732 0.22 +1.2 4,143,031 - Pf1N1B4_3732 0.22 -0.1 4,143,031 - Pf1N1B4_3732 0.22 +0.9 4,143,031 - Pf1N1B4_3732 0.22 -0.2 4,143,031 - Pf1N1B4_3732 0.22 -0.4 4,143,059 + Pf1N1B4_3732 0.24 -1.0 4,143,060 - -1.4 4,143,196 + Pf1N1B4_3732 0.36 +0.0 4,143,202 + Pf1N1B4_3732 0.37 -0.7 4,143,237 + Pf1N1B4_3732 0.40 -0.8 4,143,237 + Pf1N1B4_3732 0.40 -0.3 4,143,384 + Pf1N1B4_3732 0.53 +1.3 4,143,491 + Pf1N1B4_3732 0.62 +1.8 4,143,491 + Pf1N1B4_3732 0.62 +1.0 4,143,611 + Pf1N1B4_3732 0.73 +0.0 4,143,611 + Pf1N1B4_3732 0.73 +0.3 4,143,612 - Pf1N1B4_3732 0.73 -1.0 4,143,721 + Pf1N1B4_3732 0.82 -1.0 4,143,722 - -0.4 4,143,725 + Pf1N1B4_3732 0.83 +0.0 4,143,725 + Pf1N1B4_3732 0.83 +0.9 4,143,726 - -0.4 4,144,153 + Pf1N1B4_3733 0.13 -0.4 4,144,154 - Pf1N1B4_3733 0.13 +1.0 4,144,161 + Pf1N1B4_3733 0.13 +1.1 4,144,179 + Pf1N1B4_3733 0.14 -0.4 4,144,179 + Pf1N1B4_3733 0.14 -0.1 4,144,179 + Pf1N1B4_3733 0.14 -1.2 4,144,179 + Pf1N1B4_3733 0.14 +1.1 4,144,180 - Pf1N1B4_3733 0.14 +0.0 4,144,180 - Pf1N1B4_3733 0.14 +1.4 4,144,180 - Pf1N1B4_3733 0.14 +0.0 4,144,293 + Pf1N1B4_3733 0.20 -0.4 4,144,293 + Pf1N1B4_3733 0.20 +0.6 4,144,293 + +1.0 4,144,294 - Pf1N1B4_3733 0.20 +2.0 4,144,297 + Pf1N1B4_3733 0.20 +0.1 4,144,297 + Pf1N1B4_3733 0.20 +0.1 4,144,297 + Pf1N1B4_3733 0.20 +0.8 4,144,407 + Pf1N1B4_3733 0.26 -0.4 4,144,407 + Pf1N1B4_3733 0.26 -0.5 4,144,408 - Pf1N1B4_3733 0.26 +0.4 4,144,441 + Pf1N1B4_3733 0.28 +0.6 4,144,475 + Pf1N1B4_3733 0.30 +0.8 4,144,475 + Pf1N1B4_3733 0.30 +0.4 4,144,475 + Pf1N1B4_3733 0.30 -0.4 4,144,476 - Pf1N1B4_3733 0.30 +0.4 4,144,476 - Pf1N1B4_3733 0.30 +1.4 4,144,476 - Pf1N1B4_3733 0.30 -0.2 4,144,476 - Pf1N1B4_3733 0.30 -1.4 4,144,476 - Pf1N1B4_3733 0.30 -1.4 4,144,476 - Pf1N1B4_3733 0.30 -0.7
Or see this region's nucleotide sequence