Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3465 and Pf1N1B4_3466 are separated by 63 nucleotides Pf1N1B4_3466 and Pf1N1B4_3467 are separated by 88 nucleotides Pf1N1B4_3467 and Pf1N1B4_3468 are separated by 47 nucleotides Pf1N1B4_3468 and Pf1N1B4_3469 are separated by 169 nucleotides
Pf1N1B4_3465 - Transporter, MFS superfamily, at 3,871,796 to 3,873,178
_3465
Pf1N1B4_3466 - FIG00955605: hypothetical protein, at 3,873,242 to 3,873,988
_3466
Pf1N1B4_3467 - Flagellar biosynthesis protein FlgN, at 3,874,077 to 3,874,544
_3467
Pf1N1B4_3468 - Negative regulator of flagellin synthesis FlgM, at 3,874,592 to 3,874,918
_3468
Pf1N1B4_3469 - Flagellar basal-body P-ring formation protein FlgA, at 3,875,088 to 3,875,786
_3469
Position (kb)
3874
3875 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3873.240 kb on + strand at 3873.288 kb on + strand at 3873.288 kb on + strand at 3873.289 kb on - strand at 3873.289 kb on - strand at 3873.289 kb on - strand at 3873.385 kb on - strand, within Pf1N1B4_3466 at 3873.624 kb on + strand at 3873.625 kb on - strand, within Pf1N1B4_3466 at 3873.654 kb on + strand, within Pf1N1B4_3466 at 3873.716 kb on + strand, within Pf1N1B4_3466 at 3873.837 kb on + strand, within Pf1N1B4_3466 at 3873.838 kb on - strand, within Pf1N1B4_3466 at 3873.934 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.012 kb on - strand at 3874.012 kb on - strand at 3874.012 kb on - strand at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.136 kb on - strand, within Pf1N1B4_3467 at 3874.136 kb on - strand, within Pf1N1B4_3467 at 3874.220 kb on + strand, within Pf1N1B4_3467 at 3874.527 kb on + strand at 3874.527 kb on + strand at 3874.532 kb on + strand at 3874.532 kb on + strand at 3874.533 kb on - strand at 3874.649 kb on - strand, within Pf1N1B4_3468 at 3874.650 kb on + strand, within Pf1N1B4_3468 at 3874.650 kb on + strand, within Pf1N1B4_3468 at 3874.861 kb on + strand, within Pf1N1B4_3468 at 3874.936 kb on + strand at 3874.991 kb on - strand at 3875.089 kb on + strand at 3875.089 kb on + strand at 3875.089 kb on + strand at 3875.089 kb on + strand at 3875.090 kb on - strand at 3875.090 kb on - strand at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.298 kb on - strand, within Pf1N1B4_3469 at 3875.298 kb on - strand, within Pf1N1B4_3469 at 3875.407 kb on - strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.479 kb on - strand, within Pf1N1B4_3469 at 3875.479 kb on - strand, within Pf1N1B4_3469 at 3875.490 kb on + strand, within Pf1N1B4_3469
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 3,873,240 + -2.1 3,873,288 + -0.8 3,873,288 + +0.6 3,873,289 - +1.2 3,873,289 - +0.3 3,873,289 - +0.2 3,873,385 - Pf1N1B4_3466 0.19 -0.8 3,873,624 + -0.3 3,873,625 - Pf1N1B4_3466 0.51 +0.3 3,873,654 + Pf1N1B4_3466 0.55 -1.3 3,873,716 + Pf1N1B4_3466 0.63 -0.0 3,873,837 + Pf1N1B4_3466 0.80 +0.0 3,873,838 - Pf1N1B4_3466 0.80 -1.1 3,873,934 + -0.6 3,874,011 + +0.2 3,874,011 + +1.2 3,874,011 + -0.4 3,874,011 + -0.8 3,874,011 + +0.4 3,874,011 + -0.8 3,874,011 + +2.2 3,874,011 + +1.5 3,874,011 + +1.2 3,874,011 + +0.3 3,874,011 + +0.4 3,874,011 + -1.0 3,874,011 + +0.7 3,874,011 + -0.3 3,874,011 + -0.7 3,874,011 + +0.3 3,874,011 + +0.1 3,874,011 + -1.3 3,874,011 + -1.8 3,874,011 + -1.3 3,874,011 + -1.6 3,874,012 - -1.3 3,874,012 - -1.6 3,874,012 - +0.7 3,874,135 + Pf1N1B4_3467 0.12 -0.8 3,874,135 + Pf1N1B4_3467 0.12 -1.8 3,874,135 + Pf1N1B4_3467 0.12 +0.5 3,874,135 + Pf1N1B4_3467 0.12 -1.7 3,874,136 - Pf1N1B4_3467 0.13 -1.6 3,874,136 - Pf1N1B4_3467 0.13 -2.4 3,874,220 + Pf1N1B4_3467 0.31 -2.1 3,874,527 + -1.3 3,874,527 + -0.4 3,874,532 + +0.0 3,874,532 + -1.0 3,874,533 - -0.6 3,874,649 - Pf1N1B4_3468 0.17 +0.3 3,874,650 + Pf1N1B4_3468 0.18 -1.1 3,874,650 + Pf1N1B4_3468 0.18 -1.6 3,874,861 + Pf1N1B4_3468 0.82 -0.4 3,874,936 + +0.3 3,874,991 - +0.7 3,875,089 + -0.3 3,875,089 + -1.8 3,875,089 + -0.9 3,875,089 + +0.8 3,875,090 - +0.7 3,875,090 - -0.3 3,875,297 + Pf1N1B4_3469 0.30 +1.3 3,875,297 + Pf1N1B4_3469 0.30 +0.1 3,875,297 + Pf1N1B4_3469 0.30 -0.7 3,875,297 + Pf1N1B4_3469 0.30 +0.4 3,875,298 - Pf1N1B4_3469 0.30 +0.2 3,875,298 - Pf1N1B4_3469 0.30 -0.2 3,875,407 - Pf1N1B4_3469 0.46 +2.3 3,875,478 + Pf1N1B4_3469 0.56 +0.1 3,875,478 + Pf1N1B4_3469 0.56 +0.3 3,875,478 + Pf1N1B4_3469 0.56 +0.4 3,875,478 + Pf1N1B4_3469 0.56 +1.5 3,875,479 - Pf1N1B4_3469 0.56 +0.7 3,875,479 - Pf1N1B4_3469 0.56 -0.1 3,875,490 + Pf1N1B4_3469 0.58 +1.0
Or see this region's nucleotide sequence