Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3467

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3465 and Pf1N1B4_3466 are separated by 63 nucleotidesPf1N1B4_3466 and Pf1N1B4_3467 are separated by 88 nucleotidesPf1N1B4_3467 and Pf1N1B4_3468 are separated by 47 nucleotidesPf1N1B4_3468 and Pf1N1B4_3469 are separated by 169 nucleotides Pf1N1B4_3465 - Transporter, MFS superfamily, at 3,871,796 to 3,873,178 _3465 Pf1N1B4_3466 - FIG00955605: hypothetical protein, at 3,873,242 to 3,873,988 _3466 Pf1N1B4_3467 - Flagellar biosynthesis protein FlgN, at 3,874,077 to 3,874,544 _3467 Pf1N1B4_3468 - Negative regulator of flagellin synthesis FlgM, at 3,874,592 to 3,874,918 _3468 Pf1N1B4_3469 - Flagellar basal-body P-ring formation protein FlgA, at 3,875,088 to 3,875,786 _3469 Position (kb) 3874 3875Strain fitness (log2 ratio) -2 -1 0 1 2at 3873.240 kb on + strandat 3873.288 kb on + strandat 3873.288 kb on + strandat 3873.289 kb on - strandat 3873.289 kb on - strandat 3873.289 kb on - strandat 3873.385 kb on - strand, within Pf1N1B4_3466at 3873.624 kb on + strandat 3873.625 kb on - strand, within Pf1N1B4_3466at 3873.654 kb on + strand, within Pf1N1B4_3466at 3873.716 kb on + strand, within Pf1N1B4_3466at 3873.837 kb on + strand, within Pf1N1B4_3466at 3873.838 kb on - strand, within Pf1N1B4_3466at 3873.934 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.011 kb on + strandat 3874.012 kb on - strandat 3874.012 kb on - strandat 3874.012 kb on - strandat 3874.135 kb on + strand, within Pf1N1B4_3467at 3874.135 kb on + strand, within Pf1N1B4_3467at 3874.135 kb on + strand, within Pf1N1B4_3467at 3874.135 kb on + strand, within Pf1N1B4_3467at 3874.136 kb on - strand, within Pf1N1B4_3467at 3874.136 kb on - strand, within Pf1N1B4_3467at 3874.220 kb on + strand, within Pf1N1B4_3467at 3874.527 kb on + strandat 3874.527 kb on + strandat 3874.532 kb on + strandat 3874.532 kb on + strandat 3874.533 kb on - strandat 3874.649 kb on - strand, within Pf1N1B4_3468at 3874.650 kb on + strand, within Pf1N1B4_3468at 3874.650 kb on + strand, within Pf1N1B4_3468at 3874.861 kb on + strand, within Pf1N1B4_3468at 3874.936 kb on + strandat 3874.991 kb on - strandat 3875.089 kb on + strandat 3875.089 kb on + strandat 3875.089 kb on + strandat 3875.089 kb on + strandat 3875.090 kb on - strandat 3875.090 kb on - strandat 3875.297 kb on + strand, within Pf1N1B4_3469at 3875.297 kb on + strand, within Pf1N1B4_3469at 3875.297 kb on + strand, within Pf1N1B4_3469at 3875.297 kb on + strand, within Pf1N1B4_3469at 3875.298 kb on - strand, within Pf1N1B4_3469at 3875.298 kb on - strand, within Pf1N1B4_3469at 3875.407 kb on - strand, within Pf1N1B4_3469at 3875.478 kb on + strand, within Pf1N1B4_3469at 3875.478 kb on + strand, within Pf1N1B4_3469at 3875.478 kb on + strand, within Pf1N1B4_3469at 3875.478 kb on + strand, within Pf1N1B4_3469at 3875.479 kb on - strand, within Pf1N1B4_3469at 3875.479 kb on - strand, within Pf1N1B4_3469at 3875.490 kb on + strand, within Pf1N1B4_3469

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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3,873,240 + -2.1
3,873,288 + -0.8
3,873,288 + +0.6
3,873,289 - +1.2
3,873,289 - +0.3
3,873,289 - +0.2
3,873,385 - Pf1N1B4_3466 0.19 -0.8
3,873,624 + -0.3
3,873,625 - Pf1N1B4_3466 0.51 +0.3
3,873,654 + Pf1N1B4_3466 0.55 -1.3
3,873,716 + Pf1N1B4_3466 0.63 -0.0
3,873,837 + Pf1N1B4_3466 0.80 +0.0
3,873,838 - Pf1N1B4_3466 0.80 -1.1
3,873,934 + -0.6
3,874,011 + +0.2
3,874,011 + +1.2
3,874,011 + -0.4
3,874,011 + -0.8
3,874,011 + +0.4
3,874,011 + -0.8
3,874,011 + +2.2
3,874,011 + +1.5
3,874,011 + +1.2
3,874,011 + +0.3
3,874,011 + +0.4
3,874,011 + -1.0
3,874,011 + +0.7
3,874,011 + -0.3
3,874,011 + -0.7
3,874,011 + +0.3
3,874,011 + +0.1
3,874,011 + -1.3
3,874,011 + -1.8
3,874,011 + -1.3
3,874,011 + -1.6
3,874,012 - -1.3
3,874,012 - -1.6
3,874,012 - +0.7
3,874,135 + Pf1N1B4_3467 0.12 -0.8
3,874,135 + Pf1N1B4_3467 0.12 -1.8
3,874,135 + Pf1N1B4_3467 0.12 +0.5
3,874,135 + Pf1N1B4_3467 0.12 -1.7
3,874,136 - Pf1N1B4_3467 0.13 -1.6
3,874,136 - Pf1N1B4_3467 0.13 -2.4
3,874,220 + Pf1N1B4_3467 0.31 -2.1
3,874,527 + -1.3
3,874,527 + -0.4
3,874,532 + +0.0
3,874,532 + -1.0
3,874,533 - -0.6
3,874,649 - Pf1N1B4_3468 0.17 +0.3
3,874,650 + Pf1N1B4_3468 0.18 -1.1
3,874,650 + Pf1N1B4_3468 0.18 -1.6
3,874,861 + Pf1N1B4_3468 0.82 -0.4
3,874,936 + +0.3
3,874,991 - +0.7
3,875,089 + -0.3
3,875,089 + -1.8
3,875,089 + -0.9
3,875,089 + +0.8
3,875,090 - +0.7
3,875,090 - -0.3
3,875,297 + Pf1N1B4_3469 0.30 +1.3
3,875,297 + Pf1N1B4_3469 0.30 +0.1
3,875,297 + Pf1N1B4_3469 0.30 -0.7
3,875,297 + Pf1N1B4_3469 0.30 +0.4
3,875,298 - Pf1N1B4_3469 0.30 +0.2
3,875,298 - Pf1N1B4_3469 0.30 -0.2
3,875,407 - Pf1N1B4_3469 0.46 +2.3
3,875,478 + Pf1N1B4_3469 0.56 +0.1
3,875,478 + Pf1N1B4_3469 0.56 +0.3
3,875,478 + Pf1N1B4_3469 0.56 +0.4
3,875,478 + Pf1N1B4_3469 0.56 +1.5
3,875,479 - Pf1N1B4_3469 0.56 +0.7
3,875,479 - Pf1N1B4_3469 0.56 -0.1
3,875,490 + Pf1N1B4_3469 0.58 +1.0

Or see this region's nucleotide sequence