Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3273 and Pf1N1B4_3274 are separated by 307 nucleotides Pf1N1B4_3274 and Pf1N1B4_3275 are separated by 64 nucleotides Pf1N1B4_3275 and Pf1N1B4_3276 are separated by 30 nucleotides Pf1N1B4_3276 and Pf1N1B4_3277 overlap by 135 nucleotides
Pf1N1B4_3273 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 3,662,633 to 3,662,893
_3273
Pf1N1B4_3274 - Fosfomycin resistance protein FosA, at 3,663,201 to 3,663,617
_3274
Pf1N1B4_3275 - L-lysine permease, at 3,663,682 to 3,664,320
_3275
Pf1N1B4_3276 - hypothetical protein, at 3,664,351 to 3,664,500
_3276
Pf1N1B4_3277 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 3,664,366 to 3,665,772
_3277
Position (kb)
3663
3664
3665 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3663.139 kb on + strand at 3663.139 kb on + strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.156 kb on - strand at 3663.156 kb on - strand at 3663.162 kb on + strand at 3663.162 kb on + strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.184 kb on + strand at 3663.184 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.191 kb on + strand at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.315 kb on + strand, within Pf1N1B4_3274 at 3663.316 kb on - strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.388 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand at 3663.586 kb on - strand at 3663.640 kb on + strand at 3663.640 kb on + strand at 3663.640 kb on + strand at 3663.641 kb on - strand at 3663.641 kb on - strand at 3663.727 kb on + strand at 3663.727 kb on + strand at 3663.727 kb on + strand at 3663.728 kb on - strand at 3663.728 kb on - strand at 3663.866 kb on + strand, within Pf1N1B4_3275 at 3663.925 kb on + strand, within Pf1N1B4_3275 at 3663.925 kb on + strand, within Pf1N1B4_3275 at 3663.926 kb on - strand, within Pf1N1B4_3275 at 3664.072 kb on - strand, within Pf1N1B4_3275 at 3664.072 kb on - strand, within Pf1N1B4_3275 at 3664.162 kb on + strand, within Pf1N1B4_3275 at 3664.167 kb on + strand, within Pf1N1B4_3275 at 3664.175 kb on + strand, within Pf1N1B4_3275 at 3664.176 kb on - strand, within Pf1N1B4_3275 at 3664.210 kb on + strand, within Pf1N1B4_3275 at 3664.211 kb on - strand, within Pf1N1B4_3275 at 3664.211 kb on - strand, within Pf1N1B4_3275 at 3664.211 kb on - strand, within Pf1N1B4_3275 at 3664.211 kb on - strand at 3664.251 kb on - strand, within Pf1N1B4_3275 at 3664.346 kb on + strand at 3664.346 kb on + strand at 3664.347 kb on - strand at 3664.347 kb on - strand at 3664.347 kb on - strand at 3664.347 kb on - strand at 3664.365 kb on + strand at 3664.366 kb on - strand at 3664.456 kb on + strand at 3664.456 kb on + strand at 3664.456 kb on + strand at 3664.456 kb on + strand at 3664.457 kb on - strand at 3664.457 kb on - strand at 3664.495 kb on - strand at 3664.511 kb on + strand, within Pf1N1B4_3277 at 3664.512 kb on - strand at 3664.589 kb on + strand, within Pf1N1B4_3277 at 3664.589 kb on + strand, within Pf1N1B4_3277 at 3664.590 kb on - strand, within Pf1N1B4_3277 at 3664.590 kb on - strand, within Pf1N1B4_3277 at 3664.804 kb on + strand at 3664.804 kb on + strand, within Pf1N1B4_3277 at 3664.804 kb on + strand, within Pf1N1B4_3277 at 3664.805 kb on - strand, within Pf1N1B4_3277 at 3664.805 kb on - strand, within Pf1N1B4_3277 at 3664.805 kb on - strand, within Pf1N1B4_3277 at 3665.191 kb on + strand, within Pf1N1B4_3277 at 3665.194 kb on - strand, within Pf1N1B4_3277 at 3665.194 kb on - strand, within Pf1N1B4_3277 at 3665.194 kb on - strand, within Pf1N1B4_3277 at 3665.221 kb on + strand, within Pf1N1B4_3277 at 3665.221 kb on + strand, within Pf1N1B4_3277 at 3665.221 kb on + strand, within Pf1N1B4_3277 at 3665.222 kb on - strand, within Pf1N1B4_3277 at 3665.222 kb on - strand, within Pf1N1B4_3277
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 3,662,707 + Pf1N1B4_3273 0.28 +0.4 3,662,707 + Pf1N1B4_3273 0.28 +0.8 3,662,708 - Pf1N1B4_3273 0.29 -1.2 3,662,708 - Pf1N1B4_3273 0.29 +0.4 3,662,708 - Pf1N1B4_3273 0.29 -1.2 3,663,139 + +0.5 3,663,139 + +0.0 3,663,140 - -0.1 3,663,140 - +0.2 3,663,140 - -0.3 3,663,140 - -0.4 3,663,140 - -0.4 3,663,156 - +1.0 3,663,156 - +0.3 3,663,162 + +1.8 3,663,162 + -1.5 3,663,163 - +0.2 3,663,163 - -0.1 3,663,163 - +0.8 3,663,184 + -1.2 3,663,184 + +0.8 3,663,186 + +0.5 3,663,186 + -0.1 3,663,186 + -1.2 3,663,186 + -0.2 3,663,186 + +0.0 3,663,187 - +0.0 3,663,187 - -0.2 3,663,187 - +1.4 3,663,187 - +0.0 3,663,187 - +0.4 3,663,187 - +0.2 3,663,191 + +0.3 3,663,289 + Pf1N1B4_3274 0.21 +0.3 3,663,289 + Pf1N1B4_3274 0.21 -0.4 3,663,290 - Pf1N1B4_3274 0.21 +0.8 3,663,290 - Pf1N1B4_3274 0.21 +1.3 3,663,290 - Pf1N1B4_3274 0.21 -0.2 3,663,315 + Pf1N1B4_3274 0.27 +0.0 3,663,316 - Pf1N1B4_3274 0.28 -1.1 3,663,387 + Pf1N1B4_3274 0.45 +1.4 3,663,387 + Pf1N1B4_3274 0.45 -0.2 3,663,388 - Pf1N1B4_3274 0.45 +0.4 3,663,502 - Pf1N1B4_3274 0.72 +0.4 3,663,502 - +0.8 3,663,586 - -0.5 3,663,640 + +0.4 3,663,640 + +2.0 3,663,640 + -1.5 3,663,641 - +1.8 3,663,641 - +0.4 3,663,727 + -0.4 3,663,727 + -1.8 3,663,727 + -0.1 3,663,728 - -1.5 3,663,728 - -0.0 3,663,866 + Pf1N1B4_3275 0.29 +0.0 3,663,925 + Pf1N1B4_3275 0.38 -2.2 3,663,925 + Pf1N1B4_3275 0.38 +0.3 3,663,926 - Pf1N1B4_3275 0.38 -0.6 3,664,072 - Pf1N1B4_3275 0.61 +0.4 3,664,072 - Pf1N1B4_3275 0.61 -0.7 3,664,162 + Pf1N1B4_3275 0.75 +0.6 3,664,167 + Pf1N1B4_3275 0.76 -1.5 3,664,175 + Pf1N1B4_3275 0.77 -1.4 3,664,176 - Pf1N1B4_3275 0.77 -1.8 3,664,210 + Pf1N1B4_3275 0.83 -2.4 3,664,211 - Pf1N1B4_3275 0.83 +0.2 3,664,211 - Pf1N1B4_3275 0.83 -1.2 3,664,211 - Pf1N1B4_3275 0.83 -0.8 3,664,211 - -0.2 3,664,251 - Pf1N1B4_3275 0.89 +0.8 3,664,346 + -1.2 3,664,346 + -1.0 3,664,347 - +0.8 3,664,347 - +1.8 3,664,347 - -0.2 3,664,347 - +1.2 3,664,365 + -0.6 3,664,366 - -1.0 3,664,456 + -0.2 3,664,456 + -0.8 3,664,456 + +0.0 3,664,456 + -1.0 3,664,457 - -0.4 3,664,457 - +0.3 3,664,495 - +1.1 3,664,511 + Pf1N1B4_3277 0.10 +0.8 3,664,512 - -0.2 3,664,589 + Pf1N1B4_3277 0.16 -0.6 3,664,589 + Pf1N1B4_3277 0.16 +1.0 3,664,590 - Pf1N1B4_3277 0.16 +1.3 3,664,590 - Pf1N1B4_3277 0.16 +0.6 3,664,804 + +2.4 3,664,804 + Pf1N1B4_3277 0.31 +1.0 3,664,804 + Pf1N1B4_3277 0.31 -0.4 3,664,805 - Pf1N1B4_3277 0.31 +0.2 3,664,805 - Pf1N1B4_3277 0.31 -0.0 3,664,805 - Pf1N1B4_3277 0.31 +0.3 3,665,191 + Pf1N1B4_3277 0.59 +2.8 3,665,194 - Pf1N1B4_3277 0.59 -0.8 3,665,194 - Pf1N1B4_3277 0.59 -1.2 3,665,194 - Pf1N1B4_3277 0.59 +1.6 3,665,221 + Pf1N1B4_3277 0.61 -1.4 3,665,221 + Pf1N1B4_3277 0.61 -0.2 3,665,221 + Pf1N1B4_3277 0.61 +0.5 3,665,222 - Pf1N1B4_3277 0.61 +1.1 3,665,222 - Pf1N1B4_3277 0.61 -1.0
Or see this region's nucleotide sequence