Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3109

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3108 and Pf1N1B4_3109 are separated by 8 nucleotidesPf1N1B4_3109 and Pf1N1B4_3110 are separated by 212 nucleotidesPf1N1B4_3110 and Pf1N1B4_3111 overlap by 4 nucleotides Pf1N1B4_3108 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 3,495,058 to 3,496,170 _3108 Pf1N1B4_3109 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,496,179 to 3,497,024 _3109 Pf1N1B4_3110 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 3,497,237 to 3,497,710 _3110 Pf1N1B4_3111 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,497,707 to 3,498,765 _3111 Position (kb) 3496 3497 3498Strain fitness (log2 ratio) -2 -1 0 1 2at 3495.183 kb on + strand, within Pf1N1B4_3108at 3495.193 kb on + strand, within Pf1N1B4_3108at 3495.193 kb on + strand, within Pf1N1B4_3108at 3495.193 kb on + strand, within Pf1N1B4_3108at 3495.193 kb on + strand, within Pf1N1B4_3108at 3495.193 kb on + strand, within Pf1N1B4_3108at 3495.193 kb on + strand, within Pf1N1B4_3108at 3495.194 kb on - strand, within Pf1N1B4_3108at 3495.194 kb on - strandat 3495.222 kb on + strand, within Pf1N1B4_3108at 3495.322 kb on + strand, within Pf1N1B4_3108at 3495.323 kb on - strandat 3495.323 kb on - strand, within Pf1N1B4_3108at 3495.463 kb on + strandat 3495.463 kb on + strand, within Pf1N1B4_3108at 3495.463 kb on + strand, within Pf1N1B4_3108at 3495.464 kb on - strand, within Pf1N1B4_3108at 3495.471 kb on + strand, within Pf1N1B4_3108at 3495.529 kb on - strand, within Pf1N1B4_3108at 3495.551 kb on + strand, within Pf1N1B4_3108at 3495.551 kb on + strand, within Pf1N1B4_3108at 3495.551 kb on + strand, within Pf1N1B4_3108at 3495.551 kb on + strand, within Pf1N1B4_3108at 3495.551 kb on + strand, within Pf1N1B4_3108at 3495.551 kb on + strand, within Pf1N1B4_3108at 3495.792 kb on + strand, within Pf1N1B4_3108at 3496.036 kb on + strand, within Pf1N1B4_3108at 3496.036 kb on + strand, within Pf1N1B4_3108at 3496.036 kb on + strand, within Pf1N1B4_3108at 3496.036 kb on + strand, within Pf1N1B4_3108at 3496.037 kb on - strand, within Pf1N1B4_3108at 3496.037 kb on - strand, within Pf1N1B4_3108at 3496.075 kb on - strandat 3496.107 kb on + strandat 3496.108 kb on - strandat 3496.193 kb on + strandat 3496.194 kb on - strandat 3496.194 kb on - strandat 3496.194 kb on - strandat 3496.194 kb on - strandat 3496.194 kb on - strandat 3496.281 kb on + strand, within Pf1N1B4_3109at 3496.281 kb on + strand, within Pf1N1B4_3109at 3496.281 kb on + strand, within Pf1N1B4_3109at 3496.282 kb on - strand, within Pf1N1B4_3109at 3496.326 kb on + strand, within Pf1N1B4_3109at 3496.500 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strandat 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.551 kb on + strandat 3496.551 kb on + strand, within Pf1N1B4_3109at 3496.552 kb on - strandat 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.552 kb on - strand, within Pf1N1B4_3109at 3496.959 kb on + strandat 3496.959 kb on + strandat 3496.959 kb on + strandat 3496.959 kb on + strandat 3496.959 kb on + strandat 3496.959 kb on + strandat 3496.959 kb on + strandat 3496.959 kb on + strandat 3496.960 kb on - strandat 3497.014 kb on + strandat 3497.022 kb on + strandat 3497.181 kb on + strandat 3497.181 kb on + strandat 3497.182 kb on - strandat 3497.200 kb on + strandat 3497.200 kb on + strandat 3497.201 kb on - strandat 3497.201 kb on - strandat 3497.201 kb on - strandat 3497.741 kb on - strandat 3497.811 kb on + strandat 3497.933 kb on - strand, within Pf1N1B4_3111

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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3,495,183 + Pf1N1B4_3108 0.11 +0.4
3,495,193 + Pf1N1B4_3108 0.12 +0.1
3,495,193 + Pf1N1B4_3108 0.12 +0.9
3,495,193 + Pf1N1B4_3108 0.12 +0.4
3,495,193 + Pf1N1B4_3108 0.12 -1.6
3,495,193 + Pf1N1B4_3108 0.12 -0.1
3,495,193 + Pf1N1B4_3108 0.12 -0.4
3,495,194 - Pf1N1B4_3108 0.12 -0.4
3,495,194 - -0.1
3,495,222 + Pf1N1B4_3108 0.15 -0.1
3,495,322 + Pf1N1B4_3108 0.24 -0.7
3,495,323 - -0.1
3,495,323 - Pf1N1B4_3108 0.24 +0.2
3,495,463 + -0.1
3,495,463 + Pf1N1B4_3108 0.36 +0.1
3,495,463 + Pf1N1B4_3108 0.36 -0.1
3,495,464 - Pf1N1B4_3108 0.36 +0.5
3,495,471 + Pf1N1B4_3108 0.37 -0.1
3,495,529 - Pf1N1B4_3108 0.42 -1.5
3,495,551 + Pf1N1B4_3108 0.44 +1.8
3,495,551 + Pf1N1B4_3108 0.44 -1.1
3,495,551 + Pf1N1B4_3108 0.44 +0.3
3,495,551 + Pf1N1B4_3108 0.44 +2.7
3,495,551 + Pf1N1B4_3108 0.44 -0.1
3,495,551 + Pf1N1B4_3108 0.44 -0.1
3,495,792 + Pf1N1B4_3108 0.66 -0.7
3,496,036 + Pf1N1B4_3108 0.88 +0.3
3,496,036 + Pf1N1B4_3108 0.88 +0.1
3,496,036 + Pf1N1B4_3108 0.88 +0.5
3,496,036 + Pf1N1B4_3108 0.88 -0.4
3,496,037 - Pf1N1B4_3108 0.88 +0.1
3,496,037 - Pf1N1B4_3108 0.88 +0.4
3,496,075 - +0.9
3,496,107 + +1.5
3,496,108 - -1.3
3,496,193 + -0.6
3,496,194 - -0.1
3,496,194 - +1.1
3,496,194 - +1.3
3,496,194 - +1.9
3,496,194 - -1.5
3,496,281 + Pf1N1B4_3109 0.12 -1.7
3,496,281 + Pf1N1B4_3109 0.12 -0.6
3,496,281 + Pf1N1B4_3109 0.12 -1.4
3,496,282 - Pf1N1B4_3109 0.12 -1.5
3,496,326 + Pf1N1B4_3109 0.17 -0.7
3,496,500 + Pf1N1B4_3109 0.38 +0.4
3,496,551 + Pf1N1B4_3109 0.44 -1.7
3,496,551 + -0.7
3,496,551 + Pf1N1B4_3109 0.44 +0.9
3,496,551 + Pf1N1B4_3109 0.44 -0.4
3,496,551 + Pf1N1B4_3109 0.44 +0.1
3,496,551 + Pf1N1B4_3109 0.44 -0.0
3,496,551 + Pf1N1B4_3109 0.44 +0.9
3,496,551 + Pf1N1B4_3109 0.44 -1.1
3,496,551 + +0.3
3,496,551 + Pf1N1B4_3109 0.44 -1.1
3,496,552 - +1.9
3,496,552 - Pf1N1B4_3109 0.44 -0.1
3,496,552 - Pf1N1B4_3109 0.44 -1.7
3,496,552 - Pf1N1B4_3109 0.44 -0.9
3,496,552 - Pf1N1B4_3109 0.44 -1.7
3,496,552 - Pf1N1B4_3109 0.44 +1.9
3,496,552 - Pf1N1B4_3109 0.44 -0.7
3,496,552 - Pf1N1B4_3109 0.44 -1.1
3,496,552 - Pf1N1B4_3109 0.44 -0.7
3,496,552 - Pf1N1B4_3109 0.44 -0.9
3,496,959 + +0.2
3,496,959 + -1.4
3,496,959 + -1.5
3,496,959 + -1.1
3,496,959 + -2.3
3,496,959 + -0.1
3,496,959 + +0.9
3,496,959 + -2.1
3,496,960 - -0.1
3,497,014 + +0.0
3,497,022 + +1.3
3,497,181 + +1.1
3,497,181 + -2.6
3,497,182 - -1.5
3,497,200 + -0.6
3,497,200 + +0.3
3,497,201 - +0.9
3,497,201 - +0.3
3,497,201 - +1.9
3,497,741 - -0.0
3,497,811 + +1.2
3,497,933 - Pf1N1B4_3111 0.21 +1.9

Or see this region's nucleotide sequence