Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3108 and Pf1N1B4_3109 are separated by 8 nucleotides Pf1N1B4_3109 and Pf1N1B4_3110 are separated by 212 nucleotides Pf1N1B4_3110 and Pf1N1B4_3111 overlap by 4 nucleotides
Pf1N1B4_3108 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 3,495,058 to 3,496,170
_3108
Pf1N1B4_3109 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,496,179 to 3,497,024
_3109
Pf1N1B4_3110 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 3,497,237 to 3,497,710
_3110
Pf1N1B4_3111 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,497,707 to 3,498,765
_3111
Position (kb)
3496
3497
3498 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3495.183 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.193 kb on + strand, within Pf1N1B4_3108 at 3495.194 kb on - strand, within Pf1N1B4_3108 at 3495.194 kb on - strand at 3495.222 kb on + strand, within Pf1N1B4_3108 at 3495.322 kb on + strand, within Pf1N1B4_3108 at 3495.323 kb on - strand at 3495.323 kb on - strand, within Pf1N1B4_3108 at 3495.463 kb on + strand at 3495.463 kb on + strand, within Pf1N1B4_3108 at 3495.463 kb on + strand, within Pf1N1B4_3108 at 3495.464 kb on - strand, within Pf1N1B4_3108 at 3495.471 kb on + strand, within Pf1N1B4_3108 at 3495.529 kb on - strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.551 kb on + strand, within Pf1N1B4_3108 at 3495.792 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.036 kb on + strand, within Pf1N1B4_3108 at 3496.037 kb on - strand, within Pf1N1B4_3108 at 3496.037 kb on - strand, within Pf1N1B4_3108 at 3496.075 kb on - strand at 3496.107 kb on + strand at 3496.108 kb on - strand at 3496.193 kb on + strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.194 kb on - strand at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.281 kb on + strand, within Pf1N1B4_3109 at 3496.282 kb on - strand, within Pf1N1B4_3109 at 3496.326 kb on + strand, within Pf1N1B4_3109 at 3496.500 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.551 kb on + strand at 3496.551 kb on + strand, within Pf1N1B4_3109 at 3496.552 kb on - strand at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.552 kb on - strand, within Pf1N1B4_3109 at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.960 kb on - strand at 3497.014 kb on + strand at 3497.022 kb on + strand at 3497.181 kb on + strand at 3497.181 kb on + strand at 3497.182 kb on - strand at 3497.200 kb on + strand at 3497.200 kb on + strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.741 kb on - strand at 3497.811 kb on + strand at 3497.933 kb on - strand, within Pf1N1B4_3111
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 3,495,183 + Pf1N1B4_3108 0.11 +0.4 3,495,193 + Pf1N1B4_3108 0.12 +0.1 3,495,193 + Pf1N1B4_3108 0.12 +0.9 3,495,193 + Pf1N1B4_3108 0.12 +0.4 3,495,193 + Pf1N1B4_3108 0.12 -1.6 3,495,193 + Pf1N1B4_3108 0.12 -0.1 3,495,193 + Pf1N1B4_3108 0.12 -0.4 3,495,194 - Pf1N1B4_3108 0.12 -0.4 3,495,194 - -0.1 3,495,222 + Pf1N1B4_3108 0.15 -0.1 3,495,322 + Pf1N1B4_3108 0.24 -0.7 3,495,323 - -0.1 3,495,323 - Pf1N1B4_3108 0.24 +0.2 3,495,463 + -0.1 3,495,463 + Pf1N1B4_3108 0.36 +0.1 3,495,463 + Pf1N1B4_3108 0.36 -0.1 3,495,464 - Pf1N1B4_3108 0.36 +0.5 3,495,471 + Pf1N1B4_3108 0.37 -0.1 3,495,529 - Pf1N1B4_3108 0.42 -1.5 3,495,551 + Pf1N1B4_3108 0.44 +1.8 3,495,551 + Pf1N1B4_3108 0.44 -1.1 3,495,551 + Pf1N1B4_3108 0.44 +0.3 3,495,551 + Pf1N1B4_3108 0.44 +2.7 3,495,551 + Pf1N1B4_3108 0.44 -0.1 3,495,551 + Pf1N1B4_3108 0.44 -0.1 3,495,792 + Pf1N1B4_3108 0.66 -0.7 3,496,036 + Pf1N1B4_3108 0.88 +0.3 3,496,036 + Pf1N1B4_3108 0.88 +0.1 3,496,036 + Pf1N1B4_3108 0.88 +0.5 3,496,036 + Pf1N1B4_3108 0.88 -0.4 3,496,037 - Pf1N1B4_3108 0.88 +0.1 3,496,037 - Pf1N1B4_3108 0.88 +0.4 3,496,075 - +0.9 3,496,107 + +1.5 3,496,108 - -1.3 3,496,193 + -0.6 3,496,194 - -0.1 3,496,194 - +1.1 3,496,194 - +1.3 3,496,194 - +1.9 3,496,194 - -1.5 3,496,281 + Pf1N1B4_3109 0.12 -1.7 3,496,281 + Pf1N1B4_3109 0.12 -0.6 3,496,281 + Pf1N1B4_3109 0.12 -1.4 3,496,282 - Pf1N1B4_3109 0.12 -1.5 3,496,326 + Pf1N1B4_3109 0.17 -0.7 3,496,500 + Pf1N1B4_3109 0.38 +0.4 3,496,551 + Pf1N1B4_3109 0.44 -1.7 3,496,551 + -0.7 3,496,551 + Pf1N1B4_3109 0.44 +0.9 3,496,551 + Pf1N1B4_3109 0.44 -0.4 3,496,551 + Pf1N1B4_3109 0.44 +0.1 3,496,551 + Pf1N1B4_3109 0.44 -0.0 3,496,551 + Pf1N1B4_3109 0.44 +0.9 3,496,551 + Pf1N1B4_3109 0.44 -1.1 3,496,551 + +0.3 3,496,551 + Pf1N1B4_3109 0.44 -1.1 3,496,552 - +1.9 3,496,552 - Pf1N1B4_3109 0.44 -0.1 3,496,552 - Pf1N1B4_3109 0.44 -1.7 3,496,552 - Pf1N1B4_3109 0.44 -0.9 3,496,552 - Pf1N1B4_3109 0.44 -1.7 3,496,552 - Pf1N1B4_3109 0.44 +1.9 3,496,552 - Pf1N1B4_3109 0.44 -0.7 3,496,552 - Pf1N1B4_3109 0.44 -1.1 3,496,552 - Pf1N1B4_3109 0.44 -0.7 3,496,552 - Pf1N1B4_3109 0.44 -0.9 3,496,959 + +0.2 3,496,959 + -1.4 3,496,959 + -1.5 3,496,959 + -1.1 3,496,959 + -2.3 3,496,959 + -0.1 3,496,959 + +0.9 3,496,959 + -2.1 3,496,960 - -0.1 3,497,014 + +0.0 3,497,022 + +1.3 3,497,181 + +1.1 3,497,181 + -2.6 3,497,182 - -1.5 3,497,200 + -0.6 3,497,200 + +0.3 3,497,201 - +0.9 3,497,201 - +0.3 3,497,201 - +1.9 3,497,741 - -0.0 3,497,811 + +1.2 3,497,933 - Pf1N1B4_3111 0.21 +1.9
Or see this region's nucleotide sequence