Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_298

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_297 and Pf1N1B4_298 are separated by 91 nucleotidesPf1N1B4_298 and Pf1N1B4_299 are separated by 67 nucleotides Pf1N1B4_297 - FIG00809136: hypothetical protein, at 349,975 to 352,479 _297 Pf1N1B4_298 - Transcription elongation factor GreB, at 352,571 to 353,044 _298 Pf1N1B4_299 - HIPA PROTEIN, at 353,112 to 354,389 _299 Position (kb) 352 353 354Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 351.773 kb on + strand, within Pf1N1B4_297at 351.782 kb on + strand, within Pf1N1B4_297at 351.782 kb on + strand, within Pf1N1B4_297at 351.795 kb on + strand, within Pf1N1B4_297at 351.795 kb on + strand, within Pf1N1B4_297at 351.796 kb on - strand, within Pf1N1B4_297at 351.796 kb on - strand, within Pf1N1B4_297at 351.839 kb on + strand, within Pf1N1B4_297at 351.839 kb on + strand, within Pf1N1B4_297at 351.839 kb on + strand, within Pf1N1B4_297at 351.839 kb on + strand, within Pf1N1B4_297at 351.839 kb on + strand, within Pf1N1B4_297at 351.839 kb on + strand, within Pf1N1B4_297at 351.840 kb on - strand, within Pf1N1B4_297at 351.840 kb on - strand, within Pf1N1B4_297at 351.840 kb on - strandat 351.840 kb on - strand, within Pf1N1B4_297at 351.967 kb on + strand, within Pf1N1B4_297at 351.967 kb on + strand, within Pf1N1B4_297at 351.967 kb on + strand, within Pf1N1B4_297at 351.967 kb on + strand, within Pf1N1B4_297at 351.968 kb on - strand, within Pf1N1B4_297at 351.968 kb on - strandat 351.982 kb on + strand, within Pf1N1B4_297at 351.982 kb on + strand, within Pf1N1B4_297at 351.982 kb on + strand, within Pf1N1B4_297at 351.982 kb on + strand, within Pf1N1B4_297at 351.982 kb on + strand, within Pf1N1B4_297at 351.982 kb on + strand, within Pf1N1B4_297at 351.983 kb on - strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strandat 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.114 kb on + strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.115 kb on - strand, within Pf1N1B4_297at 352.330 kb on + strandat 352.480 kb on + strandat 352.480 kb on + strandat 352.480 kb on + strandat 352.493 kb on + strandat 352.493 kb on + strandat 352.493 kb on + strandat 352.493 kb on + strandat 352.493 kb on + strandat 352.493 kb on + strandat 352.494 kb on - strandat 352.494 kb on - strandat 352.565 kb on + strandat 352.566 kb on - strandat 352.566 kb on - strandat 352.566 kb on - strandat 352.566 kb on - strandat 352.566 kb on - strandat 352.566 kb on - strandat 352.573 kb on - strandat 352.573 kb on - strandat 352.573 kb on - strandat 352.695 kb on + strand, within Pf1N1B4_298at 352.695 kb on + strand, within Pf1N1B4_298at 352.696 kb on - strand, within Pf1N1B4_298at 352.696 kb on - strand, within Pf1N1B4_298at 352.696 kb on - strand, within Pf1N1B4_298at 352.816 kb on - strandat 352.896 kb on + strandat 352.896 kb on + strand, within Pf1N1B4_298at 352.897 kb on - strand, within Pf1N1B4_298at 352.903 kb on - strand, within Pf1N1B4_298at 353.039 kb on - strandat 353.067 kb on + strandat 353.068 kb on - strandat 353.068 kb on - strandat 353.068 kb on - strandat 353.082 kb on + strandat 353.082 kb on + strandat 353.082 kb on + strandat 353.082 kb on + strandat 353.082 kb on + strandat 353.082 kb on + strandat 353.082 kb on + strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.083 kb on - strandat 353.084 kb on + strandat 353.085 kb on - strandat 353.138 kb on + strandat 353.139 kb on - strandat 353.232 kb on + strandat 353.232 kb on + strandat 353.233 kb on - strandat 353.233 kb on - strandat 353.233 kb on - strandat 353.233 kb on - strandat 353.233 kb on - strandat 353.236 kb on + strandat 353.236 kb on + strandat 353.236 kb on + strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.237 kb on - strandat 353.254 kb on + strand, within Pf1N1B4_299at 353.255 kb on - strand, within Pf1N1B4_299at 353.255 kb on - strand, within Pf1N1B4_299at 353.255 kb on - strand, within Pf1N1B4_299at 353.260 kb on + strand, within Pf1N1B4_299at 353.260 kb on + strand, within Pf1N1B4_299at 353.260 kb on + strand, within Pf1N1B4_299at 353.261 kb on - strand, within Pf1N1B4_299at 353.301 kb on - strand, within Pf1N1B4_299at 353.351 kb on + strand, within Pf1N1B4_299at 353.351 kb on + strand, within Pf1N1B4_299at 353.351 kb on + strand, within Pf1N1B4_299at 353.351 kb on + strand, within Pf1N1B4_299at 353.352 kb on - strand, within Pf1N1B4_299at 353.352 kb on - strand, within Pf1N1B4_299at 353.352 kb on - strandat 353.352 kb on - strand, within Pf1N1B4_299at 353.352 kb on - strand, within Pf1N1B4_299at 353.352 kb on - strand, within Pf1N1B4_299at 353.352 kb on - strand, within Pf1N1B4_299at 353.359 kb on + strand, within Pf1N1B4_299at 353.414 kb on - strand, within Pf1N1B4_299at 353.447 kb on - strand, within Pf1N1B4_299at 353.447 kb on - strand, within Pf1N1B4_299at 353.447 kb on - strand, within Pf1N1B4_299at 353.535 kb on + strand, within Pf1N1B4_299at 353.536 kb on - strand, within Pf1N1B4_299at 353.536 kb on - strand, within Pf1N1B4_299at 353.546 kb on - strand, within Pf1N1B4_299at 353.546 kb on - strand, within Pf1N1B4_299at 353.546 kb on - strand, within Pf1N1B4_299at 353.595 kb on - strand, within Pf1N1B4_299at 353.600 kb on + strand, within Pf1N1B4_299at 353.601 kb on - strand, within Pf1N1B4_299at 353.601 kb on - strand, within Pf1N1B4_299at 353.601 kb on - strand, within Pf1N1B4_299at 353.601 kb on - strand, within Pf1N1B4_299at 353.694 kb on - strand, within Pf1N1B4_299at 353.703 kb on - strand, within Pf1N1B4_299at 353.734 kb on + strand, within Pf1N1B4_299at 353.803 kb on + strand, within Pf1N1B4_299at 353.803 kb on + strand, within Pf1N1B4_299at 353.911 kb on - strand, within Pf1N1B4_299at 353.946 kb on + strand, within Pf1N1B4_299at 353.946 kb on + strand, within Pf1N1B4_299at 353.946 kb on + strandat 353.946 kb on + strand, within Pf1N1B4_299at 353.946 kb on + strand, within Pf1N1B4_299at 353.947 kb on - strand, within Pf1N1B4_299at 353.947 kb on - strand, within Pf1N1B4_299at 353.947 kb on - strand, within Pf1N1B4_299at 353.947 kb on - strand, within Pf1N1B4_299at 353.947 kb on - strand, within Pf1N1B4_299at 353.967 kb on + strand, within Pf1N1B4_299at 353.967 kb on + strand, within Pf1N1B4_299at 353.967 kb on + strand, within Pf1N1B4_299at 354.019 kb on + strand, within Pf1N1B4_299at 354.020 kb on - strand, within Pf1N1B4_299

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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351,773 + Pf1N1B4_297 0.72 -0.8
351,782 + Pf1N1B4_297 0.72 +0.8
351,782 + Pf1N1B4_297 0.72 -0.2
351,795 + Pf1N1B4_297 0.73 -0.9
351,795 + Pf1N1B4_297 0.73 +0.6
351,796 - Pf1N1B4_297 0.73 +0.3
351,796 - Pf1N1B4_297 0.73 +0.3
351,839 + Pf1N1B4_297 0.74 -0.0
351,839 + Pf1N1B4_297 0.74 +0.1
351,839 + Pf1N1B4_297 0.74 -0.4
351,839 + Pf1N1B4_297 0.74 +1.2
351,839 + Pf1N1B4_297 0.74 +1.5
351,839 + Pf1N1B4_297 0.74 +0.2
351,840 - Pf1N1B4_297 0.74 -1.2
351,840 - Pf1N1B4_297 0.74 -0.9
351,840 - +2.2
351,840 - Pf1N1B4_297 0.74 -0.4
351,967 + Pf1N1B4_297 0.80 -1.6
351,967 + Pf1N1B4_297 0.80 +0.1
351,967 + Pf1N1B4_297 0.80 -0.4
351,967 + Pf1N1B4_297 0.80 +0.3
351,968 - Pf1N1B4_297 0.80 +0.1
351,968 - -0.7
351,982 + Pf1N1B4_297 0.80 -1.1
351,982 + Pf1N1B4_297 0.80 +1.4
351,982 + Pf1N1B4_297 0.80 +1.2
351,982 + Pf1N1B4_297 0.80 +0.2
351,982 + Pf1N1B4_297 0.80 +0.2
351,982 + Pf1N1B4_297 0.80 -0.3
351,983 - Pf1N1B4_297 0.80 -2.2
352,114 + Pf1N1B4_297 0.85 +0.6
352,114 + Pf1N1B4_297 0.85 +0.1
352,114 + Pf1N1B4_297 0.85 +0.6
352,114 + +0.6
352,114 + Pf1N1B4_297 0.85 -0.8
352,114 + Pf1N1B4_297 0.85 -1.8
352,114 + Pf1N1B4_297 0.85 +0.8
352,114 + Pf1N1B4_297 0.85 -0.2
352,114 + Pf1N1B4_297 0.85 -0.2
352,114 + Pf1N1B4_297 0.85 -0.9
352,114 + Pf1N1B4_297 0.85 -0.2
352,114 + Pf1N1B4_297 0.85 -0.2
352,114 + Pf1N1B4_297 0.85 -0.4
352,114 + Pf1N1B4_297 0.85 +0.8
352,114 + Pf1N1B4_297 0.85 +1.1
352,115 - Pf1N1B4_297 0.85 -1.9
352,115 - Pf1N1B4_297 0.85 +1.2
352,115 - Pf1N1B4_297 0.85 -2.8
352,115 - Pf1N1B4_297 0.85 -0.3
352,115 - Pf1N1B4_297 0.85 +0.9
352,115 - Pf1N1B4_297 0.85 +1.1
352,115 - Pf1N1B4_297 0.85 +0.6
352,115 - Pf1N1B4_297 0.85 -0.9
352,115 - Pf1N1B4_297 0.85 +0.1
352,115 - Pf1N1B4_297 0.85 +0.6
352,115 - Pf1N1B4_297 0.85 +0.5
352,115 - Pf1N1B4_297 0.85 -0.4
352,330 + -0.2
352,480 + +0.1
352,480 + +0.1
352,480 + -0.7
352,493 + +1.2
352,493 + -0.1
352,493 + -0.2
352,493 + -0.1
352,493 + +0.5
352,493 + -0.4
352,494 - +0.6
352,494 - -1.7
352,565 + +0.3
352,566 - -0.1
352,566 - -0.4
352,566 - +0.2
352,566 - +0.1
352,566 - -0.7
352,566 - +0.2
352,573 - +0.2
352,573 - -0.6
352,573 - +0.2
352,695 + Pf1N1B4_298 0.26 -1.9
352,695 + Pf1N1B4_298 0.26 -0.4
352,696 - Pf1N1B4_298 0.26 -0.7
352,696 - Pf1N1B4_298 0.26 +0.4
352,696 - Pf1N1B4_298 0.26 +0.6
352,816 - +1.6
352,896 + +1.2
352,896 + Pf1N1B4_298 0.69 -0.7
352,897 - Pf1N1B4_298 0.69 -2.4
352,903 - Pf1N1B4_298 0.70 +0.1
353,039 - -0.0
353,067 + -0.4
353,068 - +1.1
353,068 - -0.9
353,068 - +0.1
353,082 + +0.6
353,082 + -1.4
353,082 + -0.0
353,082 + +1.2
353,082 + +0.6
353,082 + +1.1
353,082 + -0.9
353,083 - -0.8
353,083 - -0.9
353,083 - +0.9
353,083 - +0.1
353,083 - +1.1
353,083 - -0.1
353,083 - -0.0
353,083 - +0.6
353,083 - +0.9
353,084 + +1.2
353,085 - -0.1
353,138 + -0.2
353,139 - -1.4
353,232 + -1.4
353,232 + +0.1
353,233 - +1.0
353,233 - -0.4
353,233 - -0.0
353,233 - +0.2
353,233 - +0.3
353,236 + +0.2
353,236 + -0.1
353,236 + -0.9
353,237 - -0.2
353,237 - +0.4
353,237 - -0.4
353,237 - -1.5
353,237 - -0.4
353,237 - -0.8
353,237 - +1.4
353,237 - +0.9
353,237 - -1.4
353,254 + Pf1N1B4_299 0.11 +0.2
353,255 - Pf1N1B4_299 0.11 -1.9
353,255 - Pf1N1B4_299 0.11 -0.9
353,255 - Pf1N1B4_299 0.11 +0.1
353,260 + Pf1N1B4_299 0.12 -0.2
353,260 + Pf1N1B4_299 0.12 -0.5
353,260 + Pf1N1B4_299 0.12 -0.1
353,261 - Pf1N1B4_299 0.12 +0.4
353,301 - Pf1N1B4_299 0.15 +1.5
353,351 + Pf1N1B4_299 0.19 +0.5
353,351 + Pf1N1B4_299 0.19 -0.5
353,351 + Pf1N1B4_299 0.19 -0.9
353,351 + Pf1N1B4_299 0.19 -0.4
353,352 - Pf1N1B4_299 0.19 -0.7
353,352 - Pf1N1B4_299 0.19 +0.2
353,352 - -1.4
353,352 - Pf1N1B4_299 0.19 +0.6
353,352 - Pf1N1B4_299 0.19 -0.4
353,352 - Pf1N1B4_299 0.19 -1.4
353,352 - Pf1N1B4_299 0.19 +0.1
353,359 + Pf1N1B4_299 0.19 +0.6
353,414 - Pf1N1B4_299 0.24 -1.4
353,447 - Pf1N1B4_299 0.26 +0.3
353,447 - Pf1N1B4_299 0.26 -0.7
353,447 - Pf1N1B4_299 0.26 +0.1
353,535 + Pf1N1B4_299 0.33 -0.4
353,536 - Pf1N1B4_299 0.33 +0.6
353,536 - Pf1N1B4_299 0.33 +0.4
353,546 - Pf1N1B4_299 0.34 +0.1
353,546 - Pf1N1B4_299 0.34 -1.5
353,546 - Pf1N1B4_299 0.34 -0.9
353,595 - Pf1N1B4_299 0.38 -0.9
353,600 + Pf1N1B4_299 0.38 +1.0
353,601 - Pf1N1B4_299 0.38 +0.0
353,601 - Pf1N1B4_299 0.38 +1.2
353,601 - Pf1N1B4_299 0.38 -1.2
353,601 - Pf1N1B4_299 0.38 -0.4
353,694 - Pf1N1B4_299 0.46 +0.6
353,703 - Pf1N1B4_299 0.46 -1.2
353,734 + Pf1N1B4_299 0.49 -1.4
353,803 + Pf1N1B4_299 0.54 +1.0
353,803 + Pf1N1B4_299 0.54 -0.9
353,911 - Pf1N1B4_299 0.63 +0.8
353,946 + Pf1N1B4_299 0.65 +0.4
353,946 + Pf1N1B4_299 0.65 +1.1
353,946 + +1.6
353,946 + Pf1N1B4_299 0.65 +0.2
353,946 + Pf1N1B4_299 0.65 +1.6
353,947 - Pf1N1B4_299 0.65 +1.1
353,947 - Pf1N1B4_299 0.65 -1.2
353,947 - Pf1N1B4_299 0.65 -0.7
353,947 - Pf1N1B4_299 0.65 +2.0
353,947 - Pf1N1B4_299 0.65 +0.4
353,967 + Pf1N1B4_299 0.67 +0.8
353,967 + Pf1N1B4_299 0.67 +0.4
353,967 + Pf1N1B4_299 0.67 -1.9
354,019 + Pf1N1B4_299 0.71 -0.1
354,020 - Pf1N1B4_299 0.71 +0.3

Or see this region's nucleotide sequence