Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2764 and Pf1N1B4_2765 are separated by 42 nucleotides Pf1N1B4_2765 and Pf1N1B4_2766 are separated by 285 nucleotides Pf1N1B4_2766 and Pf1N1B4_2767 are separated by 57 nucleotides Pf1N1B4_2767 and Pf1N1B4_2768 are separated by 45 nucleotides
Pf1N1B4_2764 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 3,103,485 to 3,104,261
_2764
Pf1N1B4_2765 - Large-conductance mechanosensitive channel, at 3,104,304 to 3,104,717
_2765
Pf1N1B4_2766 - Catalase (EC 1.11.1.6), at 3,105,003 to 3,106,544
_2766
Pf1N1B4_2767 - FOG: Ankyrin repeat, at 3,106,602 to 3,107,156
_2767
Pf1N1B4_2768 - DNA repair protein RadA, at 3,107,202 to 3,108,569
_2768
Position (kb)
3105
3106
3107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3104.098 kb on + strand, within Pf1N1B4_2764 at 3104.116 kb on + strand, within Pf1N1B4_2764 at 3104.253 kb on + strand at 3104.253 kb on + strand at 3104.254 kb on - strand at 3104.254 kb on - strand at 3104.259 kb on + strand at 3104.366 kb on + strand, within Pf1N1B4_2765 at 3104.366 kb on + strand, within Pf1N1B4_2765 at 3104.367 kb on - strand, within Pf1N1B4_2765 at 3104.367 kb on - strand, within Pf1N1B4_2765 at 3104.489 kb on + strand, within Pf1N1B4_2765 at 3104.490 kb on - strand, within Pf1N1B4_2765 at 3104.490 kb on - strand, within Pf1N1B4_2765 at 3104.490 kb on - strand, within Pf1N1B4_2765 at 3104.490 kb on - strand, within Pf1N1B4_2765 at 3104.494 kb on + strand, within Pf1N1B4_2765 at 3104.495 kb on - strand, within Pf1N1B4_2765 at 3104.506 kb on - strand, within Pf1N1B4_2765 at 3104.583 kb on + strand, within Pf1N1B4_2765 at 3104.583 kb on + strand, within Pf1N1B4_2765 at 3104.583 kb on + strand, within Pf1N1B4_2765 at 3104.583 kb on + strand, within Pf1N1B4_2765 at 3104.583 kb on + strand, within Pf1N1B4_2765 at 3104.584 kb on - strand, within Pf1N1B4_2765 at 3104.584 kb on - strand, within Pf1N1B4_2765 at 3104.584 kb on - strand, within Pf1N1B4_2765 at 3104.584 kb on - strand, within Pf1N1B4_2765 at 3104.584 kb on - strand, within Pf1N1B4_2765 at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.676 kb on - strand at 3104.706 kb on + strand at 3104.706 kb on + strand at 3104.706 kb on + strand at 3104.707 kb on - strand at 3104.707 kb on - strand at 3104.707 kb on - strand at 3104.707 kb on - strand at 3104.707 kb on - strand at 3104.707 kb on - strand at 3104.707 kb on - strand at 3104.717 kb on + strand at 3104.717 kb on + strand at 3104.718 kb on - strand at 3104.718 kb on - strand at 3104.718 kb on - strand at 3104.718 kb on - strand at 3104.718 kb on - strand at 3104.718 kb on - strand at 3104.718 kb on - strand at 3104.718 kb on - strand at 3104.722 kb on + strand at 3104.723 kb on - strand at 3104.723 kb on - strand at 3104.723 kb on - strand at 3104.733 kb on + strand at 3104.734 kb on - strand at 3104.759 kb on + strand at 3104.759 kb on + strand at 3104.760 kb on - strand at 3104.760 kb on - strand at 3104.812 kb on - strand at 3104.834 kb on - strand at 3104.834 kb on - strand at 3104.874 kb on + strand at 3104.875 kb on - strand at 3104.924 kb on + strand at 3104.925 kb on - strand at 3104.925 kb on - strand at 3104.925 kb on - strand at 3104.925 kb on - strand at 3104.925 kb on - strand at 3104.925 kb on - strand at 3104.928 kb on + strand at 3104.928 kb on + strand at 3104.928 kb on + strand at 3104.928 kb on + strand at 3104.929 kb on - strand at 3104.929 kb on - strand at 3104.929 kb on - strand at 3104.929 kb on - strand at 3104.929 kb on - strand at 3104.950 kb on + strand at 3104.983 kb on + strand at 3104.983 kb on + strand at 3104.984 kb on - strand at 3104.984 kb on - strand at 3104.984 kb on - strand at 3104.984 kb on - strand at 3105.316 kb on + strand, within Pf1N1B4_2766 at 3105.316 kb on + strand, within Pf1N1B4_2766 at 3105.317 kb on - strand, within Pf1N1B4_2766 at 3105.317 kb on - strand, within Pf1N1B4_2766 at 3105.317 kb on - strand, within Pf1N1B4_2766 at 3105.344 kb on - strand, within Pf1N1B4_2766 at 3105.427 kb on - strand, within Pf1N1B4_2766 at 3105.616 kb on - strand, within Pf1N1B4_2766 at 3105.668 kb on - strand, within Pf1N1B4_2766 at 3105.668 kb on - strand, within Pf1N1B4_2766 at 3105.668 kb on - strand, within Pf1N1B4_2766 at 3105.668 kb on - strand at 3105.676 kb on - strand, within Pf1N1B4_2766 at 3105.676 kb on - strand, within Pf1N1B4_2766 at 3105.676 kb on - strand, within Pf1N1B4_2766 at 3105.709 kb on - strand, within Pf1N1B4_2766 at 3105.709 kb on - strand, within Pf1N1B4_2766 at 3105.709 kb on - strand, within Pf1N1B4_2766 at 3105.789 kb on + strand, within Pf1N1B4_2766 at 3105.789 kb on + strand, within Pf1N1B4_2766 at 3105.797 kb on + strand, within Pf1N1B4_2766 at 3105.797 kb on + strand, within Pf1N1B4_2766 at 3105.798 kb on - strand, within Pf1N1B4_2766 at 3105.798 kb on - strand, within Pf1N1B4_2766 at 3105.798 kb on - strand, within Pf1N1B4_2766 at 3105.803 kb on + strand, within Pf1N1B4_2766 at 3105.842 kb on + strand, within Pf1N1B4_2766 at 3106.027 kb on + strand, within Pf1N1B4_2766 at 3106.028 kb on - strand, within Pf1N1B4_2766 at 3106.028 kb on - strand, within Pf1N1B4_2766 at 3106.084 kb on - strand, within Pf1N1B4_2766 at 3106.210 kb on + strand, within Pf1N1B4_2766 at 3106.210 kb on + strand, within Pf1N1B4_2766 at 3106.215 kb on + strand, within Pf1N1B4_2766 at 3106.215 kb on + strand, within Pf1N1B4_2766 at 3106.215 kb on + strand, within Pf1N1B4_2766 at 3106.216 kb on - strand, within Pf1N1B4_2766 at 3106.449 kb on + strand at 3106.449 kb on + strand at 3106.450 kb on - strand at 3106.489 kb on + strand at 3106.490 kb on - strand at 3106.705 kb on + strand, within Pf1N1B4_2767 at 3106.705 kb on + strand, within Pf1N1B4_2767 at 3106.705 kb on + strand, within Pf1N1B4_2767 at 3106.706 kb on - strand, within Pf1N1B4_2767 at 3106.706 kb on - strand, within Pf1N1B4_2767 at 3106.713 kb on + strand, within Pf1N1B4_2767 at 3106.713 kb on + strand, within Pf1N1B4_2767 at 3106.713 kb on + strand, within Pf1N1B4_2767 at 3106.713 kb on + strand, within Pf1N1B4_2767 at 3106.713 kb on + strand, within Pf1N1B4_2767 at 3106.713 kb on + strand, within Pf1N1B4_2767 at 3106.716 kb on + strand, within Pf1N1B4_2767 at 3106.716 kb on + strand, within Pf1N1B4_2767 at 3106.716 kb on + strand, within Pf1N1B4_2767 at 3106.716 kb on + strand, within Pf1N1B4_2767 at 3106.717 kb on - strand, within Pf1N1B4_2767 at 3106.717 kb on - strand, within Pf1N1B4_2767 at 3106.717 kb on - strand, within Pf1N1B4_2767 at 3106.717 kb on - strand, within Pf1N1B4_2767 at 3106.717 kb on - strand, within Pf1N1B4_2767 at 3106.717 kb on - strand, within Pf1N1B4_2767 at 3106.758 kb on - strand, within Pf1N1B4_2767 at 3106.758 kb on - strand, within Pf1N1B4_2767 at 3106.806 kb on + strand, within Pf1N1B4_2767 at 3106.830 kb on + strand, within Pf1N1B4_2767 at 3107.019 kb on + strand, within Pf1N1B4_2767 at 3107.019 kb on + strand, within Pf1N1B4_2767 at 3107.019 kb on + strand, within Pf1N1B4_2767 at 3107.020 kb on - strand, within Pf1N1B4_2767 at 3107.020 kb on - strand, within Pf1N1B4_2767 at 3107.173 kb on + strand at 3107.173 kb on + strand at 3107.173 kb on + strand at 3107.173 kb on + strand at 3107.173 kb on + strand at 3107.174 kb on - strand at 3107.174 kb on - strand at 3107.223 kb on + strand at 3107.224 kb on - strand at 3107.224 kb on - strand at 3107.224 kb on - strand at 3107.224 kb on - strand at 3107.224 kb on - strand at 3107.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 3,104,098 + Pf1N1B4_2764 0.79 -0.2 3,104,116 + Pf1N1B4_2764 0.81 -0.1 3,104,253 + +1.2 3,104,253 + +3.0 3,104,254 - -1.3 3,104,254 - +0.8 3,104,259 + +1.0 3,104,366 + Pf1N1B4_2765 0.15 +0.2 3,104,366 + Pf1N1B4_2765 0.15 +0.1 3,104,367 - Pf1N1B4_2765 0.15 +0.6 3,104,367 - Pf1N1B4_2765 0.15 -1.7 3,104,489 + Pf1N1B4_2765 0.45 +1.4 3,104,490 - Pf1N1B4_2765 0.45 -2.2 3,104,490 - Pf1N1B4_2765 0.45 -0.0 3,104,490 - Pf1N1B4_2765 0.45 -0.0 3,104,490 - Pf1N1B4_2765 0.45 +0.4 3,104,494 + Pf1N1B4_2765 0.46 -0.9 3,104,495 - Pf1N1B4_2765 0.46 -1.7 3,104,506 - Pf1N1B4_2765 0.49 +1.5 3,104,583 + Pf1N1B4_2765 0.67 +0.3 3,104,583 + Pf1N1B4_2765 0.67 -0.4 3,104,583 + Pf1N1B4_2765 0.67 -0.0 3,104,583 + Pf1N1B4_2765 0.67 +0.3 3,104,583 + Pf1N1B4_2765 0.67 +0.4 3,104,584 - Pf1N1B4_2765 0.68 +0.2 3,104,584 - Pf1N1B4_2765 0.68 +0.2 3,104,584 - Pf1N1B4_2765 0.68 -0.8 3,104,584 - Pf1N1B4_2765 0.68 -1.1 3,104,584 - Pf1N1B4_2765 0.68 +2.4 3,104,676 - +0.1 3,104,676 - -1.4 3,104,676 - +0.1 3,104,676 - -0.2 3,104,676 - +0.8 3,104,676 - -0.0 3,104,676 - +0.2 3,104,676 - +0.4 3,104,676 - -1.0 3,104,676 - -0.2 3,104,676 - -0.4 3,104,706 + +0.7 3,104,706 + -0.0 3,104,706 + -0.6 3,104,707 - -1.0 3,104,707 - -0.1 3,104,707 - +0.3 3,104,707 - -1.0 3,104,707 - -0.5 3,104,707 - -2.8 3,104,707 - +0.2 3,104,717 + -1.1 3,104,717 + +1.0 3,104,718 - -1.0 3,104,718 - -0.5 3,104,718 - +3.0 3,104,718 - +0.4 3,104,718 - -0.0 3,104,718 - -0.0 3,104,718 - +0.6 3,104,718 - +1.0 3,104,722 + +0.2 3,104,723 - +1.5 3,104,723 - -0.6 3,104,723 - -0.6 3,104,733 + -0.0 3,104,734 - -0.2 3,104,759 + -1.2 3,104,759 + -0.6 3,104,760 - -1.3 3,104,760 - -0.2 3,104,812 - -1.0 3,104,834 - -0.0 3,104,834 - -0.0 3,104,874 + -0.0 3,104,875 - -1.9 3,104,924 + -0.1 3,104,925 - +0.4 3,104,925 - +2.2 3,104,925 - +0.2 3,104,925 - +1.1 3,104,925 - -0.0 3,104,925 - -1.2 3,104,928 + -0.2 3,104,928 + +0.6 3,104,928 + -0.8 3,104,928 + -0.4 3,104,929 - +0.7 3,104,929 - +0.2 3,104,929 - +0.2 3,104,929 - -0.0 3,104,929 - -1.8 3,104,950 + -0.6 3,104,983 + +1.5 3,104,983 + +0.1 3,104,984 - -0.0 3,104,984 - +0.2 3,104,984 - -0.0 3,104,984 - -0.2 3,105,316 + Pf1N1B4_2766 0.20 +0.6 3,105,316 + Pf1N1B4_2766 0.20 -1.8 3,105,317 - Pf1N1B4_2766 0.20 -0.6 3,105,317 - Pf1N1B4_2766 0.20 -0.4 3,105,317 - Pf1N1B4_2766 0.20 +0.2 3,105,344 - Pf1N1B4_2766 0.22 -0.0 3,105,427 - Pf1N1B4_2766 0.27 -1.6 3,105,616 - Pf1N1B4_2766 0.40 -0.8 3,105,668 - Pf1N1B4_2766 0.43 +0.5 3,105,668 - Pf1N1B4_2766 0.43 -0.8 3,105,668 - Pf1N1B4_2766 0.43 -0.5 3,105,668 - +1.6 3,105,676 - Pf1N1B4_2766 0.44 -0.0 3,105,676 - Pf1N1B4_2766 0.44 +2.2 3,105,676 - Pf1N1B4_2766 0.44 +1.0 3,105,709 - Pf1N1B4_2766 0.46 +1.6 3,105,709 - Pf1N1B4_2766 0.46 -0.0 3,105,709 - Pf1N1B4_2766 0.46 +1.2 3,105,789 + Pf1N1B4_2766 0.51 -0.0 3,105,789 + Pf1N1B4_2766 0.51 -0.2 3,105,797 + Pf1N1B4_2766 0.51 -1.0 3,105,797 + Pf1N1B4_2766 0.51 +0.2 3,105,798 - Pf1N1B4_2766 0.52 -0.9 3,105,798 - Pf1N1B4_2766 0.52 -0.6 3,105,798 - Pf1N1B4_2766 0.52 +1.0 3,105,803 + Pf1N1B4_2766 0.52 -0.8 3,105,842 + Pf1N1B4_2766 0.54 -1.7 3,106,027 + Pf1N1B4_2766 0.66 -0.0 3,106,028 - Pf1N1B4_2766 0.66 -1.0 3,106,028 - Pf1N1B4_2766 0.66 +2.0 3,106,084 - Pf1N1B4_2766 0.70 +1.0 3,106,210 + Pf1N1B4_2766 0.78 -1.4 3,106,210 + Pf1N1B4_2766 0.78 +1.2 3,106,215 + Pf1N1B4_2766 0.79 -0.6 3,106,215 + Pf1N1B4_2766 0.79 +1.0 3,106,215 + Pf1N1B4_2766 0.79 -2.3 3,106,216 - Pf1N1B4_2766 0.79 +0.2 3,106,449 + -1.6 3,106,449 + +0.8 3,106,450 - -0.3 3,106,489 + +0.4 3,106,490 - +0.1 3,106,705 + Pf1N1B4_2767 0.19 +0.1 3,106,705 + Pf1N1B4_2767 0.19 -0.9 3,106,705 + Pf1N1B4_2767 0.19 -0.6 3,106,706 - Pf1N1B4_2767 0.19 -1.2 3,106,706 - Pf1N1B4_2767 0.19 +1.2 3,106,713 + Pf1N1B4_2767 0.20 +0.8 3,106,713 + Pf1N1B4_2767 0.20 -1.0 3,106,713 + Pf1N1B4_2767 0.20 -0.4 3,106,713 + Pf1N1B4_2767 0.20 +1.0 3,106,713 + Pf1N1B4_2767 0.20 +1.6 3,106,713 + Pf1N1B4_2767 0.20 -0.6 3,106,716 + Pf1N1B4_2767 0.21 -0.4 3,106,716 + Pf1N1B4_2767 0.21 +0.7 3,106,716 + Pf1N1B4_2767 0.21 +1.8 3,106,716 + Pf1N1B4_2767 0.21 -0.6 3,106,717 - Pf1N1B4_2767 0.21 -0.0 3,106,717 - Pf1N1B4_2767 0.21 +0.1 3,106,717 - Pf1N1B4_2767 0.21 -0.2 3,106,717 - Pf1N1B4_2767 0.21 +1.0 3,106,717 - Pf1N1B4_2767 0.21 +1.0 3,106,717 - Pf1N1B4_2767 0.21 +0.6 3,106,758 - Pf1N1B4_2767 0.28 +0.2 3,106,758 - Pf1N1B4_2767 0.28 +0.2 3,106,806 + Pf1N1B4_2767 0.37 +0.3 3,106,830 + Pf1N1B4_2767 0.41 -0.8 3,107,019 + Pf1N1B4_2767 0.75 -0.6 3,107,019 + Pf1N1B4_2767 0.75 -0.0 3,107,019 + Pf1N1B4_2767 0.75 -0.5 3,107,020 - Pf1N1B4_2767 0.75 -0.0 3,107,020 - Pf1N1B4_2767 0.75 -0.6 3,107,173 + -0.6 3,107,173 + -0.2 3,107,173 + -0.0 3,107,173 + +0.7 3,107,173 + +0.5 3,107,174 - -0.1 3,107,174 - -0.9 3,107,223 + -0.4 3,107,224 - +0.1 3,107,224 - +1.0 3,107,224 - -0.4 3,107,224 - -0.6 3,107,224 - -0.4 3,107,224 - +0.2
Or see this region's nucleotide sequence