Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_274 and Pf1N1B4_275 are separated by 69 nucleotides Pf1N1B4_275 and Pf1N1B4_276 are separated by 350 nucleotides Pf1N1B4_276 and Pf1N1B4_277 are separated by 32 nucleotides
Pf1N1B4_274 - Transcriptional regulator, LysR family, at 333,631 to 334,623
_274
Pf1N1B4_275 - Organic hydroperoxide resistance transcriptional regulator, at 334,693 to 335,154
_275
Pf1N1B4_276 - Organic hydroperoxide resistance protein, at 335,505 to 335,924
_276
Pf1N1B4_277 - Lipase, at 335,957 to 336,973
_277
Position (kb)
335
336 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 334.552 kb on + strand at 334.622 kb on - strand at 334.795 kb on + strand, within Pf1N1B4_275 at 334.796 kb on - strand, within Pf1N1B4_275 at 334.796 kb on - strand, within Pf1N1B4_275 at 334.828 kb on + strand, within Pf1N1B4_275 at 334.994 kb on + strand, within Pf1N1B4_275 at 334.994 kb on + strand, within Pf1N1B4_275 at 334.995 kb on - strand at 335.130 kb on + strand at 335.131 kb on - strand at 335.131 kb on - strand at 335.145 kb on + strand at 335.348 kb on + strand at 335.348 kb on + strand at 335.348 kb on + strand at 335.349 kb on - strand at 335.349 kb on - strand at 335.356 kb on + strand at 335.357 kb on - strand at 335.421 kb on - strand at 335.518 kb on - strand at 335.845 kb on + strand, within Pf1N1B4_276 at 335.845 kb on + strand, within Pf1N1B4_276 at 335.846 kb on - strand, within Pf1N1B4_276 at 335.846 kb on - strand, within Pf1N1B4_276 at 335.846 kb on - strand, within Pf1N1B4_276 at 335.922 kb on + strand at 335.922 kb on + strand at 335.923 kb on - strand at 335.923 kb on - strand at 335.923 kb on - strand at 335.923 kb on - strand at 335.956 kb on + strand at 335.956 kb on + strand at 335.956 kb on + strand at 335.957 kb on - strand at 335.957 kb on - strand at 335.957 kb on - strand at 335.957 kb on - strand at 336.063 kb on + strand, within Pf1N1B4_277 at 336.063 kb on + strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand, within Pf1N1B4_277 at 336.064 kb on - strand at 336.065 kb on - strand, within Pf1N1B4_277 at 336.147 kb on + strand, within Pf1N1B4_277 at 336.368 kb on + strand at 336.368 kb on + strand, within Pf1N1B4_277 at 336.368 kb on + strand, within Pf1N1B4_277 at 336.368 kb on + strand, within Pf1N1B4_277 at 336.368 kb on + strand, within Pf1N1B4_277 at 336.369 kb on - strand, within Pf1N1B4_277 at 336.369 kb on - strand, within Pf1N1B4_277 at 336.393 kb on - strand at 336.416 kb on + strand, within Pf1N1B4_277 at 336.417 kb on - strand, within Pf1N1B4_277 at 336.563 kb on + strand, within Pf1N1B4_277 at 336.563 kb on + strand at 336.614 kb on + strand, within Pf1N1B4_277 at 336.614 kb on + strand, within Pf1N1B4_277 at 336.614 kb on + strand at 336.614 kb on + strand, within Pf1N1B4_277 at 336.614 kb on + strand, within Pf1N1B4_277 at 336.615 kb on - strand, within Pf1N1B4_277 at 336.615 kb on - strand, within Pf1N1B4_277 at 336.615 kb on - strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.677 kb on + strand, within Pf1N1B4_277 at 336.678 kb on - strand, within Pf1N1B4_277 at 336.678 kb on - strand, within Pf1N1B4_277 at 336.678 kb on - strand, within Pf1N1B4_277 at 336.678 kb on - strand, within Pf1N1B4_277 at 336.678 kb on - strand, within Pf1N1B4_277 at 336.710 kb on + strand, within Pf1N1B4_277 at 336.710 kb on + strand, within Pf1N1B4_277 at 336.710 kb on + strand, within Pf1N1B4_277 at 336.711 kb on - strand, within Pf1N1B4_277 at 336.711 kb on - strand, within Pf1N1B4_277 at 336.711 kb on - strand, within Pf1N1B4_277 at 336.711 kb on - strand, within Pf1N1B4_277 at 336.789 kb on + strand, within Pf1N1B4_277 at 336.789 kb on + strand, within Pf1N1B4_277 at 336.789 kb on + strand, within Pf1N1B4_277 at 336.789 kb on + strand, within Pf1N1B4_277 at 336.789 kb on + strand, within Pf1N1B4_277 at 336.789 kb on + strand, within Pf1N1B4_277 at 336.790 kb on - strand, within Pf1N1B4_277 at 336.790 kb on - strand, within Pf1N1B4_277 at 336.813 kb on - strand, within Pf1N1B4_277 at 336.860 kb on + strand, within Pf1N1B4_277 at 336.861 kb on - strand at 336.861 kb on - strand, within Pf1N1B4_277 at 336.882 kb on - strand at 336.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 334,552 + +0.2 334,622 - -1.0 334,795 + Pf1N1B4_275 0.22 -1.7 334,796 - Pf1N1B4_275 0.22 -1.5 334,796 - Pf1N1B4_275 0.22 +1.0 334,828 + Pf1N1B4_275 0.29 +0.1 334,994 + Pf1N1B4_275 0.65 -0.4 334,994 + Pf1N1B4_275 0.65 +0.6 334,995 - -0.4 335,130 + -0.4 335,131 - -0.4 335,131 - +0.0 335,145 + +0.0 335,348 + -0.1 335,348 + +0.4 335,348 + +0.3 335,349 - +1.4 335,349 - -1.0 335,356 + -1.0 335,357 - +1.2 335,421 - -0.1 335,518 - -0.8 335,845 + Pf1N1B4_276 0.81 +0.4 335,845 + Pf1N1B4_276 0.81 -0.7 335,846 - Pf1N1B4_276 0.81 +0.2 335,846 - Pf1N1B4_276 0.81 +0.3 335,846 - Pf1N1B4_276 0.81 +0.1 335,922 + -0.4 335,922 + -0.0 335,923 - +0.0 335,923 - +0.6 335,923 - +0.3 335,923 - -0.4 335,956 + +0.0 335,956 + -0.5 335,956 + -1.0 335,957 - +0.6 335,957 - +0.6 335,957 - -1.0 335,957 - +0.3 336,063 + Pf1N1B4_277 0.10 -0.4 336,063 + Pf1N1B4_277 0.10 -1.0 336,064 - Pf1N1B4_277 0.11 +2.0 336,064 - Pf1N1B4_277 0.11 +0.4 336,064 - Pf1N1B4_277 0.11 +0.4 336,064 - Pf1N1B4_277 0.11 +0.3 336,064 - Pf1N1B4_277 0.11 +1.4 336,064 - +0.6 336,065 - Pf1N1B4_277 0.11 -0.1 336,147 + Pf1N1B4_277 0.19 +1.2 336,368 + +0.6 336,368 + Pf1N1B4_277 0.40 +1.2 336,368 + Pf1N1B4_277 0.40 +0.6 336,368 + Pf1N1B4_277 0.40 +0.8 336,368 + Pf1N1B4_277 0.40 -2.0 336,369 - Pf1N1B4_277 0.41 -0.6 336,369 - Pf1N1B4_277 0.41 +1.8 336,393 - -1.7 336,416 + Pf1N1B4_277 0.45 -0.3 336,417 - Pf1N1B4_277 0.45 +0.2 336,563 + Pf1N1B4_277 0.60 -0.4 336,563 + +0.0 336,614 + Pf1N1B4_277 0.65 -0.3 336,614 + Pf1N1B4_277 0.65 +0.0 336,614 + -0.4 336,614 + Pf1N1B4_277 0.65 +1.6 336,614 + Pf1N1B4_277 0.65 +0.4 336,615 - Pf1N1B4_277 0.65 -0.6 336,615 - Pf1N1B4_277 0.65 -0.1 336,615 - Pf1N1B4_277 0.65 +0.8 336,677 + Pf1N1B4_277 0.71 +0.7 336,677 + Pf1N1B4_277 0.71 -0.4 336,677 + Pf1N1B4_277 0.71 -0.1 336,677 + Pf1N1B4_277 0.71 -0.1 336,677 + Pf1N1B4_277 0.71 -0.0 336,677 + Pf1N1B4_277 0.71 -0.1 336,677 + -0.1 336,677 + Pf1N1B4_277 0.71 +0.6 336,677 + Pf1N1B4_277 0.71 -0.5 336,677 + Pf1N1B4_277 0.71 -0.4 336,677 + Pf1N1B4_277 0.71 -0.4 336,678 - Pf1N1B4_277 0.71 -0.1 336,678 - Pf1N1B4_277 0.71 -0.5 336,678 - Pf1N1B4_277 0.71 +0.4 336,678 - Pf1N1B4_277 0.71 +0.6 336,678 - Pf1N1B4_277 0.71 +0.8 336,710 + Pf1N1B4_277 0.74 +1.1 336,710 + Pf1N1B4_277 0.74 +1.4 336,710 + Pf1N1B4_277 0.74 +0.8 336,711 - Pf1N1B4_277 0.74 +0.6 336,711 - Pf1N1B4_277 0.74 +0.3 336,711 - Pf1N1B4_277 0.74 +0.4 336,711 - Pf1N1B4_277 0.74 +1.2 336,789 + Pf1N1B4_277 0.82 -1.2 336,789 + Pf1N1B4_277 0.82 +0.4 336,789 + Pf1N1B4_277 0.82 +0.1 336,789 + Pf1N1B4_277 0.82 +1.9 336,789 + Pf1N1B4_277 0.82 +0.6 336,789 + Pf1N1B4_277 0.82 +0.2 336,790 - Pf1N1B4_277 0.82 -0.9 336,790 - Pf1N1B4_277 0.82 -0.4 336,813 - Pf1N1B4_277 0.84 -0.4 336,860 + Pf1N1B4_277 0.89 -0.4 336,861 - +0.6 336,861 - Pf1N1B4_277 0.89 +0.9 336,882 - +0.2 336,882 - -0.2
Or see this region's nucleotide sequence