Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_257 and Pf1N1B4_258 overlap by 17 nucleotides Pf1N1B4_258 and Pf1N1B4_259 overlap by 4 nucleotides Pf1N1B4_259 and Pf1N1B4_260 are separated by 187 nucleotides
Pf1N1B4_257 - sensor histidine kinase/response regulator, at 316,478 to 318,841
_257
Pf1N1B4_258 - Organic hydroperoxide resistance transcriptional regulator, at 318,825 to 319,286
_258
Pf1N1B4_259 - Glutathione peroxidase family protein, at 319,283 to 319,768
_259
Pf1N1B4_260 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 319,956 to 320,351
_260
Position (kb) 318
319
320 Strain fitness (log2 ratio) -2
-1
0
1
2 at 317.924 kb on - strand, within Pf1N1B4_257 at 317.966 kb on + strand, within Pf1N1B4_257 at 317.966 kb on + strand, within Pf1N1B4_257 at 317.966 kb on + strand, within Pf1N1B4_257 at 317.966 kb on + strand, within Pf1N1B4_257 at 317.966 kb on + strand, within Pf1N1B4_257 at 317.966 kb on + strand, within Pf1N1B4_257 at 317.967 kb on - strand at 317.967 kb on - strand, within Pf1N1B4_257 at 317.968 kb on + strand, within Pf1N1B4_257 at 317.968 kb on + strand, within Pf1N1B4_257 at 317.969 kb on - strand, within Pf1N1B4_257 at 318.266 kb on - strand at 318.384 kb on - strand, within Pf1N1B4_257 at 318.384 kb on - strand, within Pf1N1B4_257 at 318.645 kb on - strand at 318.826 kb on + strand at 318.913 kb on - strand, within Pf1N1B4_258 at 319.157 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.280 kb on + strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.509 kb on + strand, within Pf1N1B4_259 at 319.611 kb on + strand, within Pf1N1B4_259 at 319.611 kb on + strand, within Pf1N1B4_259 at 319.611 kb on + strand, within Pf1N1B4_259 at 319.612 kb on - strand, within Pf1N1B4_259 at 319.641 kb on + strand, within Pf1N1B4_259 at 319.664 kb on - strand, within Pf1N1B4_259 at 319.857 kb on + strand at 319.857 kb on + strand at 319.958 kb on - strand at 319.958 kb on - strand at 319.999 kb on + strand, within Pf1N1B4_260 at 320.000 kb on - strand, within Pf1N1B4_260 at 320.140 kb on + strand, within Pf1N1B4_260 at 320.141 kb on - strand, within Pf1N1B4_260 at 320.141 kb on - strand, within Pf1N1B4_260 at 320.245 kb on + strand, within Pf1N1B4_260 at 320.245 kb on + strand at 320.245 kb on + strand, within Pf1N1B4_260
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 317,924 - Pf1N1B4_257 0.61 -0.6 317,966 + Pf1N1B4_257 0.63 +0.6 317,966 + Pf1N1B4_257 0.63 +0.0 317,966 + Pf1N1B4_257 0.63 +0.2 317,966 + Pf1N1B4_257 0.63 -0.8 317,966 + Pf1N1B4_257 0.63 -1.0 317,966 + Pf1N1B4_257 0.63 -0.4 317,967 - +0.2 317,967 - Pf1N1B4_257 0.63 -0.1 317,968 + Pf1N1B4_257 0.63 -0.4 317,968 + Pf1N1B4_257 0.63 -1.2 317,969 - Pf1N1B4_257 0.63 +0.0 318,266 - +0.6 318,384 - Pf1N1B4_257 0.81 +0.1 318,384 - Pf1N1B4_257 0.81 -2.7 318,645 - +0.3 318,826 + -0.4 318,913 - Pf1N1B4_258 0.19 +0.6 319,157 - Pf1N1B4_258 0.72 -1.4 319,191 - Pf1N1B4_258 0.79 -1.2 319,191 - Pf1N1B4_258 0.79 +1.6 319,191 - Pf1N1B4_258 0.79 -1.7 319,191 - Pf1N1B4_258 0.79 -1.3 319,191 - Pf1N1B4_258 0.79 +0.0 319,191 - Pf1N1B4_258 0.79 +0.2 319,191 - Pf1N1B4_258 0.79 +1.0 319,191 - Pf1N1B4_258 0.79 -0.1 319,191 - Pf1N1B4_258 0.79 +0.9 319,191 - Pf1N1B4_258 0.79 -1.7 319,199 - Pf1N1B4_258 0.81 +0.3 319,199 - Pf1N1B4_258 0.81 +2.5 319,199 - Pf1N1B4_258 0.81 -0.8 319,199 - Pf1N1B4_258 0.81 +0.4 319,229 - Pf1N1B4_258 0.87 +0.8 319,229 - Pf1N1B4_258 0.87 +0.5 319,229 - Pf1N1B4_258 0.87 -0.4 319,229 - Pf1N1B4_258 0.87 -0.7 319,229 - Pf1N1B4_258 0.87 +0.6 319,280 + +1.2 319,281 - -0.1 319,281 - -1.7 319,281 - +0.6 319,281 - -0.1 319,281 - -1.1 319,281 - +1.6 319,281 - +0.2 319,509 + Pf1N1B4_259 0.47 -1.5 319,611 + Pf1N1B4_259 0.67 -2.4 319,611 + Pf1N1B4_259 0.67 -0.1 319,611 + Pf1N1B4_259 0.67 -0.7 319,612 - Pf1N1B4_259 0.68 +0.6 319,641 + Pf1N1B4_259 0.74 -1.0 319,664 - Pf1N1B4_259 0.78 -0.1 319,857 + +1.3 319,857 + -0.2 319,958 - -2.1 319,958 - -1.0 319,999 + Pf1N1B4_260 0.11 -0.2 320,000 - Pf1N1B4_260 0.11 -2.0 320,140 + Pf1N1B4_260 0.46 +0.8 320,141 - Pf1N1B4_260 0.47 -1.2 320,141 - Pf1N1B4_260 0.47 -0.1 320,245 + Pf1N1B4_260 0.73 -1.1 320,245 + +1.0 320,245 + Pf1N1B4_260 0.73 -0.1
Or see this region's nucleotide sequence