Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_258

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_257 and Pf1N1B4_258 overlap by 17 nucleotidesPf1N1B4_258 and Pf1N1B4_259 overlap by 4 nucleotidesPf1N1B4_259 and Pf1N1B4_260 are separated by 187 nucleotides Pf1N1B4_257 - sensor histidine kinase/response regulator, at 316,478 to 318,841 _257 Pf1N1B4_258 - Organic hydroperoxide resistance transcriptional regulator, at 318,825 to 319,286 _258 Pf1N1B4_259 - Glutathione peroxidase family protein, at 319,283 to 319,768 _259 Pf1N1B4_260 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 319,956 to 320,351 _260 Position (kb) 318 319 320Strain fitness (log2 ratio) -2 -1 0 1 2at 317.924 kb on - strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.966 kb on + strand, within Pf1N1B4_257at 317.967 kb on - strandat 317.967 kb on - strand, within Pf1N1B4_257at 317.968 kb on + strand, within Pf1N1B4_257at 317.968 kb on + strand, within Pf1N1B4_257at 317.969 kb on - strand, within Pf1N1B4_257at 318.266 kb on - strandat 318.384 kb on - strand, within Pf1N1B4_257at 318.384 kb on - strand, within Pf1N1B4_257at 318.645 kb on - strandat 318.826 kb on + strandat 318.913 kb on - strand, within Pf1N1B4_258at 319.157 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.280 kb on + strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.509 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.612 kb on - strand, within Pf1N1B4_259at 319.641 kb on + strand, within Pf1N1B4_259at 319.664 kb on - strand, within Pf1N1B4_259at 319.857 kb on + strandat 319.857 kb on + strandat 319.958 kb on - strandat 319.958 kb on - strandat 319.999 kb on + strand, within Pf1N1B4_260at 320.000 kb on - strand, within Pf1N1B4_260at 320.140 kb on + strand, within Pf1N1B4_260at 320.141 kb on - strand, within Pf1N1B4_260at 320.141 kb on - strand, within Pf1N1B4_260at 320.245 kb on + strand, within Pf1N1B4_260at 320.245 kb on + strandat 320.245 kb on + strand, within Pf1N1B4_260

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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317,924 - Pf1N1B4_257 0.61 -0.6
317,966 + Pf1N1B4_257 0.63 +0.6
317,966 + Pf1N1B4_257 0.63 +0.0
317,966 + Pf1N1B4_257 0.63 +0.2
317,966 + Pf1N1B4_257 0.63 -0.8
317,966 + Pf1N1B4_257 0.63 -1.0
317,966 + Pf1N1B4_257 0.63 -0.4
317,967 - +0.2
317,967 - Pf1N1B4_257 0.63 -0.1
317,968 + Pf1N1B4_257 0.63 -0.4
317,968 + Pf1N1B4_257 0.63 -1.2
317,969 - Pf1N1B4_257 0.63 +0.0
318,266 - +0.6
318,384 - Pf1N1B4_257 0.81 +0.1
318,384 - Pf1N1B4_257 0.81 -2.7
318,645 - +0.3
318,826 + -0.4
318,913 - Pf1N1B4_258 0.19 +0.6
319,157 - Pf1N1B4_258 0.72 -1.4
319,191 - Pf1N1B4_258 0.79 -1.2
319,191 - Pf1N1B4_258 0.79 +1.6
319,191 - Pf1N1B4_258 0.79 -1.7
319,191 - Pf1N1B4_258 0.79 -1.3
319,191 - Pf1N1B4_258 0.79 +0.0
319,191 - Pf1N1B4_258 0.79 +0.2
319,191 - Pf1N1B4_258 0.79 +1.0
319,191 - Pf1N1B4_258 0.79 -0.1
319,191 - Pf1N1B4_258 0.79 +0.9
319,191 - Pf1N1B4_258 0.79 -1.7
319,199 - Pf1N1B4_258 0.81 +0.3
319,199 - Pf1N1B4_258 0.81 +2.5
319,199 - Pf1N1B4_258 0.81 -0.8
319,199 - Pf1N1B4_258 0.81 +0.4
319,229 - Pf1N1B4_258 0.87 +0.8
319,229 - Pf1N1B4_258 0.87 +0.5
319,229 - Pf1N1B4_258 0.87 -0.4
319,229 - Pf1N1B4_258 0.87 -0.7
319,229 - Pf1N1B4_258 0.87 +0.6
319,280 + +1.2
319,281 - -0.1
319,281 - -1.7
319,281 - +0.6
319,281 - -0.1
319,281 - -1.1
319,281 - +1.6
319,281 - +0.2
319,509 + Pf1N1B4_259 0.47 -1.5
319,611 + Pf1N1B4_259 0.67 -2.4
319,611 + Pf1N1B4_259 0.67 -0.1
319,611 + Pf1N1B4_259 0.67 -0.7
319,612 - Pf1N1B4_259 0.68 +0.6
319,641 + Pf1N1B4_259 0.74 -1.0
319,664 - Pf1N1B4_259 0.78 -0.1
319,857 + +1.3
319,857 + -0.2
319,958 - -2.1
319,958 - -1.0
319,999 + Pf1N1B4_260 0.11 -0.2
320,000 - Pf1N1B4_260 0.11 -2.0
320,140 + Pf1N1B4_260 0.46 +0.8
320,141 - Pf1N1B4_260 0.47 -1.2
320,141 - Pf1N1B4_260 0.47 -0.1
320,245 + Pf1N1B4_260 0.73 -1.1
320,245 + +1.0
320,245 + Pf1N1B4_260 0.73 -0.1

Or see this region's nucleotide sequence