Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2421

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2420 and Pf1N1B4_2421 overlap by 4 nucleotidesPf1N1B4_2421 and Pf1N1B4_2422 are separated by 77 nucleotides Pf1N1B4_2420 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 2,745,122 to 2,746,300 _2420 Pf1N1B4_2421 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 2,746,297 to 2,747,142 _2421 Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170 _2422 Position (kb) 2746 2747 2748Strain fitness (log2 ratio) -2 -1 0 1 2at 2745.473 kb on + strand, within Pf1N1B4_2420at 2745.473 kb on + strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.474 kb on - strand, within Pf1N1B4_2420at 2745.513 kb on + strand, within Pf1N1B4_2420at 2745.514 kb on - strand, within Pf1N1B4_2420at 2745.514 kb on - strand, within Pf1N1B4_2420at 2745.555 kb on + strand, within Pf1N1B4_2420at 2746.131 kb on - strand, within Pf1N1B4_2420at 2746.312 kb on + strandat 2746.312 kb on + strandat 2746.313 kb on - strandat 2746.339 kb on - strandat 2746.364 kb on - strandat 2746.626 kb on - strand, within Pf1N1B4_2421at 2746.626 kb on - strand, within Pf1N1B4_2421at 2746.710 kb on + strand, within Pf1N1B4_2421at 2746.775 kb on + strand, within Pf1N1B4_2421at 2746.775 kb on + strand, within Pf1N1B4_2421at 2746.776 kb on - strand, within Pf1N1B4_2421at 2746.776 kb on - strandat 2746.776 kb on - strand, within Pf1N1B4_2421at 2746.776 kb on - strand, within Pf1N1B4_2421at 2746.969 kb on + strand, within Pf1N1B4_2421at 2747.108 kb on + strandat 2747.109 kb on - strandat 2747.109 kb on - strandat 2747.109 kb on - strandat 2747.122 kb on + strandat 2747.123 kb on - strandat 2747.170 kb on + strandat 2747.170 kb on + strandat 2747.170 kb on + strandat 2747.265 kb on - strandat 2747.473 kb on + strandat 2747.473 kb on + strand, within Pf1N1B4_2422at 2747.474 kb on - strand, within Pf1N1B4_2422at 2747.474 kb on - strand, within Pf1N1B4_2422at 2747.474 kb on - strand, within Pf1N1B4_2422at 2747.474 kb on - strand, within Pf1N1B4_2422at 2747.506 kb on + strand, within Pf1N1B4_2422at 2747.507 kb on - strand, within Pf1N1B4_2422at 2747.507 kb on - strand, within Pf1N1B4_2422at 2747.527 kb on + strand, within Pf1N1B4_2422at 2747.527 kb on + strand, within Pf1N1B4_2422at 2747.527 kb on + strand, within Pf1N1B4_2422at 2747.528 kb on - strand, within Pf1N1B4_2422at 2747.528 kb on - strand, within Pf1N1B4_2422at 2747.528 kb on - strand, within Pf1N1B4_2422at 2747.528 kb on - strand, within Pf1N1B4_2422at 2747.528 kb on - strand, within Pf1N1B4_2422at 2747.737 kb on + strand, within Pf1N1B4_2422at 2747.737 kb on + strand, within Pf1N1B4_2422at 2747.738 kb on - strand, within Pf1N1B4_2422at 2747.738 kb on - strand, within Pf1N1B4_2422at 2747.738 kb on - strand, within Pf1N1B4_2422at 2747.802 kb on - strand, within Pf1N1B4_2422at 2747.803 kb on + strand, within Pf1N1B4_2422at 2747.803 kb on + strand, within Pf1N1B4_2422at 2747.803 kb on + strand, within Pf1N1B4_2422at 2747.803 kb on + strand, within Pf1N1B4_2422at 2747.803 kb on + strand, within Pf1N1B4_2422at 2747.803 kb on + strand, within Pf1N1B4_2422at 2747.803 kb on + strand, within Pf1N1B4_2422at 2747.804 kb on - strand, within Pf1N1B4_2422at 2747.804 kb on - strand, within Pf1N1B4_2422at 2747.804 kb on - strand, within Pf1N1B4_2422at 2747.804 kb on - strand, within Pf1N1B4_2422at 2747.804 kb on - strand, within Pf1N1B4_2422at 2747.804 kb on - strand, within Pf1N1B4_2422at 2747.830 kb on + strand, within Pf1N1B4_2422at 2747.831 kb on - strand, within Pf1N1B4_2422at 2747.849 kb on + strand, within Pf1N1B4_2422at 2747.922 kb on - strand, within Pf1N1B4_2422at 2747.965 kb on + strand, within Pf1N1B4_2422at 2747.965 kb on + strand, within Pf1N1B4_2422at 2747.965 kb on + strand, within Pf1N1B4_2422at 2747.966 kb on - strand, within Pf1N1B4_2422at 2747.966 kb on - strand, within Pf1N1B4_2422at 2748.004 kb on + strand, within Pf1N1B4_2422at 2748.005 kb on - strand, within Pf1N1B4_2422

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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2,745,473 + Pf1N1B4_2420 0.30 -1.5
2,745,473 + Pf1N1B4_2420 0.30 -0.5
2,745,474 - Pf1N1B4_2420 0.30 -0.6
2,745,474 - Pf1N1B4_2420 0.30 +0.5
2,745,474 - Pf1N1B4_2420 0.30 +0.8
2,745,474 - Pf1N1B4_2420 0.30 +2.1
2,745,474 - Pf1N1B4_2420 0.30 -1.7
2,745,474 - Pf1N1B4_2420 0.30 -0.5
2,745,474 - Pf1N1B4_2420 0.30 -0.5
2,745,474 - Pf1N1B4_2420 0.30 +0.7
2,745,513 + Pf1N1B4_2420 0.33 +0.4
2,745,514 - Pf1N1B4_2420 0.33 -1.0
2,745,514 - Pf1N1B4_2420 0.33 +0.0
2,745,555 + Pf1N1B4_2420 0.37 +0.5
2,746,131 - Pf1N1B4_2420 0.86 -0.6
2,746,312 + -0.5
2,746,312 + -0.7
2,746,313 - +0.1
2,746,339 - +0.2
2,746,364 - +0.6
2,746,626 - Pf1N1B4_2421 0.39 +0.2
2,746,626 - Pf1N1B4_2421 0.39 +2.3
2,746,710 + Pf1N1B4_2421 0.49 -2.5
2,746,775 + Pf1N1B4_2421 0.57 -2.1
2,746,775 + Pf1N1B4_2421 0.57 -0.9
2,746,776 - Pf1N1B4_2421 0.57 -1.5
2,746,776 - -1.7
2,746,776 - Pf1N1B4_2421 0.57 -0.7
2,746,776 - Pf1N1B4_2421 0.57 -1.2
2,746,969 + Pf1N1B4_2421 0.79 +1.1
2,747,108 + -0.4
2,747,109 - +0.1
2,747,109 - -0.6
2,747,109 - -0.5
2,747,122 + -0.7
2,747,123 - -0.9
2,747,170 + -0.1
2,747,170 + -0.8
2,747,170 + +0.1
2,747,265 - +0.4
2,747,473 + +0.7
2,747,473 + Pf1N1B4_2422 0.27 +0.7
2,747,474 - Pf1N1B4_2422 0.27 -0.1
2,747,474 - Pf1N1B4_2422 0.27 +1.2
2,747,474 - Pf1N1B4_2422 0.27 +1.1
2,747,474 - Pf1N1B4_2422 0.27 -0.7
2,747,506 + Pf1N1B4_2422 0.30 +0.8
2,747,507 - Pf1N1B4_2422 0.30 -0.1
2,747,507 - Pf1N1B4_2422 0.30 -0.6
2,747,527 + Pf1N1B4_2422 0.32 -1.1
2,747,527 + Pf1N1B4_2422 0.32 +0.1
2,747,527 + Pf1N1B4_2422 0.32 +0.5
2,747,528 - Pf1N1B4_2422 0.32 -0.2
2,747,528 - Pf1N1B4_2422 0.32 -0.2
2,747,528 - Pf1N1B4_2422 0.32 +0.4
2,747,528 - Pf1N1B4_2422 0.32 +0.0
2,747,528 - Pf1N1B4_2422 0.32 +0.2
2,747,737 + Pf1N1B4_2422 0.54 -0.6
2,747,737 + Pf1N1B4_2422 0.54 -1.2
2,747,738 - Pf1N1B4_2422 0.54 +0.1
2,747,738 - Pf1N1B4_2422 0.54 -0.9
2,747,738 - Pf1N1B4_2422 0.54 -0.5
2,747,802 - Pf1N1B4_2422 0.61 +1.5
2,747,803 + Pf1N1B4_2422 0.61 -0.5
2,747,803 + Pf1N1B4_2422 0.61 -1.9
2,747,803 + Pf1N1B4_2422 0.61 +0.1
2,747,803 + Pf1N1B4_2422 0.61 -0.2
2,747,803 + Pf1N1B4_2422 0.61 -0.6
2,747,803 + Pf1N1B4_2422 0.61 +0.1
2,747,803 + Pf1N1B4_2422 0.61 +1.4
2,747,804 - Pf1N1B4_2422 0.61 -2.4
2,747,804 - Pf1N1B4_2422 0.61 +0.3
2,747,804 - Pf1N1B4_2422 0.61 -0.5
2,747,804 - Pf1N1B4_2422 0.61 -0.7
2,747,804 - Pf1N1B4_2422 0.61 -1.9
2,747,804 - Pf1N1B4_2422 0.61 +0.5
2,747,830 + Pf1N1B4_2422 0.64 -0.5
2,747,831 - Pf1N1B4_2422 0.64 -0.2
2,747,849 + Pf1N1B4_2422 0.66 -0.4
2,747,922 - Pf1N1B4_2422 0.74 +0.3
2,747,965 + Pf1N1B4_2422 0.78 -0.5
2,747,965 + Pf1N1B4_2422 0.78 -0.2
2,747,965 + Pf1N1B4_2422 0.78 +1.1
2,747,966 - Pf1N1B4_2422 0.78 +1.4
2,747,966 - Pf1N1B4_2422 0.78 -0.4
2,748,004 + Pf1N1B4_2422 0.82 -0.5
2,748,005 - Pf1N1B4_2422 0.83 -0.5

Or see this region's nucleotide sequence