Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2341 and Pf1N1B4_2342 are separated by 36 nucleotides Pf1N1B4_2342 and Pf1N1B4_2343 are separated by 56 nucleotides Pf1N1B4_2343 and Pf1N1B4_2344 are separated by 130 nucleotides
Pf1N1B4_2341 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 2,656,961 to 2,657,779
_2341
Pf1N1B4_2342 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 2,657,816 to 2,658,502
_2342
Pf1N1B4_2343 - Twitching motility protein PilT, at 2,658,559 to 2,659,593
_2343
Pf1N1B4_2344 - NLP/P60 family protein, at 2,659,724 to 2,660,347
_2344
Position (kb)
2658
2659
2660 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2657.765 kb on - strand at 2657.791 kb on - strand at 2657.791 kb on - strand at 2657.791 kb on - strand at 2657.791 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2657.793 kb on - strand at 2658.330 kb on + strand, within Pf1N1B4_2342 at 2658.331 kb on - strand, within Pf1N1B4_2342 at 2658.469 kb on + strand at 2658.470 kb on - strand at 2658.539 kb on + strand at 2658.540 kb on - strand at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.712 kb on + strand, within Pf1N1B4_2343 at 2658.713 kb on - strand, within Pf1N1B4_2343 at 2658.713 kb on - strand, within Pf1N1B4_2343 at 2659.188 kb on + strand, within Pf1N1B4_2343 at 2659.188 kb on + strand, within Pf1N1B4_2343 at 2659.189 kb on - strand, within Pf1N1B4_2343 at 2659.189 kb on - strand, within Pf1N1B4_2343 at 2659.189 kb on - strand, within Pf1N1B4_2343 at 2659.230 kb on - strand, within Pf1N1B4_2343 at 2659.462 kb on + strand, within Pf1N1B4_2343 at 2659.462 kb on + strand, within Pf1N1B4_2343 at 2659.462 kb on + strand, within Pf1N1B4_2343 at 2659.462 kb on + strand, within Pf1N1B4_2343 at 2659.462 kb on + strand, within Pf1N1B4_2343 at 2659.463 kb on - strand, within Pf1N1B4_2343 at 2659.463 kb on - strand, within Pf1N1B4_2343 at 2659.463 kb on - strand, within Pf1N1B4_2343 at 2659.509 kb on + strand at 2659.509 kb on + strand at 2659.509 kb on + strand at 2659.509 kb on + strand at 2659.510 kb on - strand at 2659.510 kb on - strand at 2659.510 kb on - strand at 2659.785 kb on + strand at 2659.785 kb on + strand at 2659.785 kb on + strand at 2659.785 kb on + strand at 2659.785 kb on + strand at 2659.785 kb on + strand at 2659.785 kb on + strand at 2659.786 kb on - strand at 2659.786 kb on - strand at 2659.786 kb on - strand at 2659.786 kb on - strand at 2659.786 kb on - strand at 2659.800 kb on + strand, within Pf1N1B4_2344 at 2659.801 kb on - strand, within Pf1N1B4_2344 at 2659.801 kb on - strand, within Pf1N1B4_2344 at 2659.875 kb on + strand, within Pf1N1B4_2344 at 2659.875 kb on + strand, within Pf1N1B4_2344 at 2659.875 kb on + strand, within Pf1N1B4_2344 at 2659.875 kb on + strand, within Pf1N1B4_2344 at 2659.875 kb on + strand, within Pf1N1B4_2344 at 2659.875 kb on + strand, within Pf1N1B4_2344 at 2659.876 kb on + strand, within Pf1N1B4_2344 at 2659.876 kb on - strand, within Pf1N1B4_2344 at 2659.876 kb on - strand, within Pf1N1B4_2344 at 2659.876 kb on - strand, within Pf1N1B4_2344 at 2659.876 kb on - strand, within Pf1N1B4_2344 at 2659.876 kb on - strand, within Pf1N1B4_2344 at 2659.876 kb on - strand, within Pf1N1B4_2344 at 2659.876 kb on - strand at 2659.990 kb on + strand, within Pf1N1B4_2344 at 2659.991 kb on - strand at 2659.991 kb on - strand, within Pf1N1B4_2344 at 2660.009 kb on - strand, within Pf1N1B4_2344 at 2660.009 kb on - strand, within Pf1N1B4_2344 at 2660.025 kb on + strand, within Pf1N1B4_2344 at 2660.025 kb on + strand, within Pf1N1B4_2344 at 2660.025 kb on + strand, within Pf1N1B4_2344 at 2660.025 kb on + strand, within Pf1N1B4_2344 at 2660.025 kb on + strand, within Pf1N1B4_2344 at 2660.025 kb on + strand, within Pf1N1B4_2344 at 2660.026 kb on - strand at 2660.026 kb on - strand, within Pf1N1B4_2344 at 2660.026 kb on - strand, within Pf1N1B4_2344 at 2660.056 kb on + strand, within Pf1N1B4_2344 at 2660.057 kb on - strand, within Pf1N1B4_2344 at 2660.057 kb on - strand, within Pf1N1B4_2344 at 2660.059 kb on + strand, within Pf1N1B4_2344 at 2660.060 kb on - strand, within Pf1N1B4_2344 at 2660.082 kb on + strand, within Pf1N1B4_2344 at 2660.089 kb on + strand, within Pf1N1B4_2344 at 2660.089 kb on + strand, within Pf1N1B4_2344 at 2660.192 kb on + strand, within Pf1N1B4_2344 at 2660.192 kb on + strand, within Pf1N1B4_2344 at 2660.193 kb on - strand, within Pf1N1B4_2344 at 2660.193 kb on - strand, within Pf1N1B4_2344 at 2660.193 kb on - strand, within Pf1N1B4_2344 at 2660.306 kb on + strand at 2660.306 kb on + strand at 2660.307 kb on - strand at 2660.307 kb on - strand at 2660.309 kb on + strand at 2660.348 kb on + strand at 2660.348 kb on + strand at 2660.348 kb on + strand at 2660.361 kb on + strand at 2660.381 kb on + strand at 2660.381 kb on + strand at 2660.381 kb on + strand at 2660.381 kb on + strand at 2660.381 kb on + strand at 2660.383 kb on + strand at 2660.442 kb on + strand at 2660.442 kb on + strand at 2660.443 kb on - strand at 2660.443 kb on - strand at 2660.446 kb on + strand at 2660.446 kb on + strand at 2660.447 kb on - strand at 2660.447 kb on - strand at 2660.486 kb on + strand at 2660.486 kb on + strand at 2660.487 kb on - strand at 2660.487 kb on - strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.489 kb on + strand at 2660.490 kb on - strand at 2660.490 kb on - strand at 2660.490 kb on - strand at 2660.490 kb on - strand at 2660.490 kb on - strand at 2660.514 kb on + strand at 2660.514 kb on + strand at 2660.515 kb on - strand at 2660.526 kb on + strand at 2660.527 kb on - strand at 2660.527 kb on - strand at 2660.527 kb on - strand at 2660.568 kb on - strand at 2660.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 2,657,765 - -1.0 2,657,791 - +0.4 2,657,791 - -0.5 2,657,791 - -0.5 2,657,791 - +0.8 2,657,793 - -0.2 2,657,793 - -0.2 2,657,793 - +0.7 2,657,793 - +2.7 2,657,793 - +0.5 2,658,330 + Pf1N1B4_2342 0.75 -0.5 2,658,331 - Pf1N1B4_2342 0.75 +0.7 2,658,469 + +0.1 2,658,470 - +0.5 2,658,539 + -1.9 2,658,540 - -0.3 2,658,712 + Pf1N1B4_2343 0.15 -0.3 2,658,712 + Pf1N1B4_2343 0.15 +0.1 2,658,712 + Pf1N1B4_2343 0.15 +0.1 2,658,712 + Pf1N1B4_2343 0.15 +0.9 2,658,712 + Pf1N1B4_2343 0.15 -0.9 2,658,713 - Pf1N1B4_2343 0.15 -0.3 2,658,713 - Pf1N1B4_2343 0.15 +2.1 2,659,188 + Pf1N1B4_2343 0.61 +0.2 2,659,188 + Pf1N1B4_2343 0.61 +0.3 2,659,189 - Pf1N1B4_2343 0.61 +1.4 2,659,189 - Pf1N1B4_2343 0.61 +0.1 2,659,189 - Pf1N1B4_2343 0.61 +0.9 2,659,230 - Pf1N1B4_2343 0.65 -0.5 2,659,462 + Pf1N1B4_2343 0.87 +0.5 2,659,462 + Pf1N1B4_2343 0.87 +1.7 2,659,462 + Pf1N1B4_2343 0.87 +0.4 2,659,462 + Pf1N1B4_2343 0.87 +1.8 2,659,462 + Pf1N1B4_2343 0.87 +1.3 2,659,463 - Pf1N1B4_2343 0.87 +0.1 2,659,463 - Pf1N1B4_2343 0.87 +0.1 2,659,463 - Pf1N1B4_2343 0.87 +0.5 2,659,509 + -1.3 2,659,509 + -0.1 2,659,509 + -0.5 2,659,509 + +0.2 2,659,510 - +1.4 2,659,510 - -0.7 2,659,510 - -0.3 2,659,785 + -0.2 2,659,785 + +0.7 2,659,785 + +2.1 2,659,785 + -1.9 2,659,785 + -1.7 2,659,785 + -1.6 2,659,785 + +0.3 2,659,786 - -0.4 2,659,786 - +0.9 2,659,786 - -0.1 2,659,786 - +1.1 2,659,786 - -1.5 2,659,800 + Pf1N1B4_2344 0.12 +0.1 2,659,801 - Pf1N1B4_2344 0.12 +0.6 2,659,801 - Pf1N1B4_2344 0.12 +1.1 2,659,875 + Pf1N1B4_2344 0.24 +0.1 2,659,875 + Pf1N1B4_2344 0.24 -0.4 2,659,875 + Pf1N1B4_2344 0.24 +0.0 2,659,875 + Pf1N1B4_2344 0.24 +0.1 2,659,875 + Pf1N1B4_2344 0.24 +0.3 2,659,875 + Pf1N1B4_2344 0.24 +1.1 2,659,876 + Pf1N1B4_2344 0.24 +0.5 2,659,876 - Pf1N1B4_2344 0.24 -1.9 2,659,876 - Pf1N1B4_2344 0.24 +0.4 2,659,876 - Pf1N1B4_2344 0.24 +0.5 2,659,876 - Pf1N1B4_2344 0.24 +0.9 2,659,876 - Pf1N1B4_2344 0.24 +0.9 2,659,876 - Pf1N1B4_2344 0.24 -0.2 2,659,876 - +1.1 2,659,990 + Pf1N1B4_2344 0.43 -0.4 2,659,991 - +0.5 2,659,991 - Pf1N1B4_2344 0.43 +0.1 2,660,009 - Pf1N1B4_2344 0.46 -0.5 2,660,009 - Pf1N1B4_2344 0.46 -2.2 2,660,025 + Pf1N1B4_2344 0.48 -0.7 2,660,025 + Pf1N1B4_2344 0.48 -0.5 2,660,025 + Pf1N1B4_2344 0.48 -0.3 2,660,025 + Pf1N1B4_2344 0.48 -0.4 2,660,025 + Pf1N1B4_2344 0.48 -0.1 2,660,025 + Pf1N1B4_2344 0.48 -0.1 2,660,026 - +0.1 2,660,026 - Pf1N1B4_2344 0.48 +0.1 2,660,026 - Pf1N1B4_2344 0.48 -0.3 2,660,056 + Pf1N1B4_2344 0.53 -0.8 2,660,057 - Pf1N1B4_2344 0.53 -0.7 2,660,057 - Pf1N1B4_2344 0.53 -0.1 2,660,059 + Pf1N1B4_2344 0.54 -1.0 2,660,060 - Pf1N1B4_2344 0.54 +0.5 2,660,082 + Pf1N1B4_2344 0.57 +0.7 2,660,089 + Pf1N1B4_2344 0.58 +0.3 2,660,089 + Pf1N1B4_2344 0.58 +0.4 2,660,192 + Pf1N1B4_2344 0.75 -0.2 2,660,192 + Pf1N1B4_2344 0.75 -1.0 2,660,193 - Pf1N1B4_2344 0.75 +1.7 2,660,193 - Pf1N1B4_2344 0.75 -0.1 2,660,193 - Pf1N1B4_2344 0.75 -1.1 2,660,306 + -0.1 2,660,306 + +0.3 2,660,307 - -2.2 2,660,307 - +0.5 2,660,309 + -0.0 2,660,348 + -0.5 2,660,348 + +1.7 2,660,348 + +0.4 2,660,361 + +0.3 2,660,381 + +0.5 2,660,381 + -0.9 2,660,381 + -0.9 2,660,381 + -0.7 2,660,381 + +0.9 2,660,383 + -0.2 2,660,442 + -0.2 2,660,442 + +0.6 2,660,443 - +0.3 2,660,443 - -0.8 2,660,446 + +0.6 2,660,446 + +0.7 2,660,447 - -0.1 2,660,447 - -0.1 2,660,486 + +0.5 2,660,486 + -0.1 2,660,487 - +1.5 2,660,487 - -0.3 2,660,489 + +0.4 2,660,489 + +0.3 2,660,489 + -0.1 2,660,489 + +0.1 2,660,489 + -0.7 2,660,489 + -0.6 2,660,489 + -0.4 2,660,489 + -0.3 2,660,489 + +0.1 2,660,489 + -0.1 2,660,489 + +0.5 2,660,489 + +0.5 2,660,489 + -0.4 2,660,489 + +1.1 2,660,489 + -1.5 2,660,489 + +0.6 2,660,490 - +0.6 2,660,490 - -0.9 2,660,490 - +1.5 2,660,490 - +0.2 2,660,490 - +0.7 2,660,514 + -0.1 2,660,514 + -0.5 2,660,515 - +0.3 2,660,526 + +0.5 2,660,527 - +1.1 2,660,527 - -1.5 2,660,527 - -0.3 2,660,568 - +0.9 2,660,568 - -0.8
Or see this region's nucleotide sequence