Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2084 and Pf1N1B4_2085 are separated by 14 nucleotides
Pf1N1B4_2084 - Uncharacterized protein ImpC, at 2,384,596 to 2,386,071
_2084
Pf1N1B4_2085 - Uncharacterized protein similar to VCA0109, at 2,386,086 to 2,386,493
_2085
Position (kb)
2386
2387 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2385.121 kb on + strand, within Pf1N1B4_2084 at 2385.121 kb on + strand, within Pf1N1B4_2084 at 2385.121 kb on + strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.122 kb on - strand, within Pf1N1B4_2084 at 2385.373 kb on + strand, within Pf1N1B4_2084 at 2385.374 kb on - strand, within Pf1N1B4_2084 at 2385.374 kb on - strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.406 kb on + strand, within Pf1N1B4_2084 at 2385.407 kb on - strand, within Pf1N1B4_2084 at 2385.407 kb on - strand, within Pf1N1B4_2084 at 2385.407 kb on - strand, within Pf1N1B4_2084 at 2385.428 kb on - strand, within Pf1N1B4_2084 at 2385.578 kb on + strand, within Pf1N1B4_2084 at 2385.578 kb on + strand, within Pf1N1B4_2084 at 2385.578 kb on + strand, within Pf1N1B4_2084 at 2385.578 kb on + strand, within Pf1N1B4_2084 at 2385.579 kb on - strand, within Pf1N1B4_2084 at 2385.601 kb on + strand, within Pf1N1B4_2084 at 2385.739 kb on + strand, within Pf1N1B4_2084 at 2385.739 kb on + strand, within Pf1N1B4_2084 at 2385.740 kb on - strand, within Pf1N1B4_2084 at 2385.740 kb on - strand, within Pf1N1B4_2084 at 2385.740 kb on - strand, within Pf1N1B4_2084 at 2385.740 kb on - strand, within Pf1N1B4_2084 at 2385.740 kb on - strand, within Pf1N1B4_2084 at 2385.740 kb on - strand, within Pf1N1B4_2084 at 2385.763 kb on + strand, within Pf1N1B4_2084 at 2385.763 kb on + strand, within Pf1N1B4_2084 at 2385.764 kb on - strand, within Pf1N1B4_2084 at 2385.764 kb on - strand, within Pf1N1B4_2084 at 2385.764 kb on - strand, within Pf1N1B4_2084 at 2385.764 kb on - strand, within Pf1N1B4_2084 at 2385.764 kb on - strand, within Pf1N1B4_2084 at 2385.793 kb on + strand, within Pf1N1B4_2084 at 2385.794 kb on - strand, within Pf1N1B4_2084 at 2385.794 kb on - strand, within Pf1N1B4_2084 at 2385.794 kb on - strand, within Pf1N1B4_2084 at 2385.841 kb on - strand, within Pf1N1B4_2084 at 2386.018 kb on + strand at 2386.018 kb on + strand at 2386.018 kb on + strand at 2386.018 kb on + strand at 2386.018 kb on + strand at 2386.018 kb on + strand at 2386.018 kb on + strand at 2386.019 kb on - strand at 2386.019 kb on - strand at 2386.019 kb on - strand at 2386.069 kb on + strand at 2386.329 kb on + strand, within Pf1N1B4_2085 at 2386.329 kb on + strand, within Pf1N1B4_2085 at 2386.330 kb on - strand, within Pf1N1B4_2085 at 2386.480 kb on + strand at 2386.481 kb on - strand at 2386.481 kb on - strand at 2386.481 kb on - strand at 2386.481 kb on - strand at 2386.481 kb on - strand at 2386.492 kb on - strand at 2386.492 kb on - strand at 2386.800 kb on - strand at 2386.800 kb on - strand at 2386.889 kb on - strand at 2386.941 kb on + strand at 2387.163 kb on - strand at 2387.253 kb on + strand at 2387.254 kb on - strand at 2387.463 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 2,385,121 + Pf1N1B4_2084 0.36 -1.5 2,385,121 + Pf1N1B4_2084 0.36 +0.2 2,385,121 + Pf1N1B4_2084 0.36 -0.4 2,385,122 - Pf1N1B4_2084 0.36 -0.7 2,385,122 - Pf1N1B4_2084 0.36 -0.5 2,385,122 - Pf1N1B4_2084 0.36 +0.5 2,385,122 - +0.2 2,385,122 - Pf1N1B4_2084 0.36 +1.2 2,385,122 - Pf1N1B4_2084 0.36 +0.2 2,385,373 + Pf1N1B4_2084 0.53 -0.6 2,385,374 - Pf1N1B4_2084 0.53 +1.7 2,385,374 - Pf1N1B4_2084 0.53 -2.0 2,385,406 + Pf1N1B4_2084 0.55 -0.6 2,385,406 + Pf1N1B4_2084 0.55 -1.1 2,385,406 + Pf1N1B4_2084 0.55 -2.0 2,385,406 + Pf1N1B4_2084 0.55 -0.5 2,385,407 - Pf1N1B4_2084 0.55 -1.5 2,385,407 - Pf1N1B4_2084 0.55 -1.8 2,385,407 - Pf1N1B4_2084 0.55 +0.9 2,385,428 - Pf1N1B4_2084 0.56 +0.9 2,385,578 + Pf1N1B4_2084 0.67 +0.2 2,385,578 + Pf1N1B4_2084 0.67 +0.9 2,385,578 + Pf1N1B4_2084 0.67 -0.8 2,385,578 + Pf1N1B4_2084 0.67 -0.8 2,385,579 - Pf1N1B4_2084 0.67 -1.4 2,385,601 + Pf1N1B4_2084 0.68 +0.2 2,385,739 + Pf1N1B4_2084 0.77 +0.4 2,385,739 + Pf1N1B4_2084 0.77 -0.1 2,385,740 - Pf1N1B4_2084 0.78 -0.5 2,385,740 - Pf1N1B4_2084 0.78 +0.1 2,385,740 - Pf1N1B4_2084 0.78 -0.1 2,385,740 - Pf1N1B4_2084 0.78 -1.1 2,385,740 - Pf1N1B4_2084 0.78 -1.0 2,385,740 - Pf1N1B4_2084 0.78 -0.4 2,385,763 + Pf1N1B4_2084 0.79 +0.6 2,385,763 + Pf1N1B4_2084 0.79 -1.4 2,385,764 - Pf1N1B4_2084 0.79 -0.1 2,385,764 - Pf1N1B4_2084 0.79 -1.4 2,385,764 - Pf1N1B4_2084 0.79 -0.6 2,385,764 - Pf1N1B4_2084 0.79 -1.3 2,385,764 - Pf1N1B4_2084 0.79 +0.2 2,385,793 + Pf1N1B4_2084 0.81 -1.0 2,385,794 - Pf1N1B4_2084 0.81 +1.2 2,385,794 - Pf1N1B4_2084 0.81 +1.2 2,385,794 - Pf1N1B4_2084 0.81 -0.4 2,385,841 - Pf1N1B4_2084 0.84 +2.2 2,386,018 + -0.2 2,386,018 + -0.2 2,386,018 + -1.1 2,386,018 + +1.2 2,386,018 + -0.1 2,386,018 + -0.1 2,386,018 + +1.2 2,386,019 - +1.2 2,386,019 - +0.9 2,386,019 - -0.3 2,386,069 + +0.8 2,386,329 + Pf1N1B4_2085 0.60 -0.4 2,386,329 + Pf1N1B4_2085 0.60 -0.8 2,386,330 - Pf1N1B4_2085 0.60 -0.3 2,386,480 + +0.2 2,386,481 - +0.8 2,386,481 - +0.8 2,386,481 - +0.2 2,386,481 - +0.4 2,386,481 - +2.8 2,386,492 - +0.2 2,386,492 - +0.1 2,386,800 - +1.5 2,386,800 - +0.7 2,386,889 - +0.4 2,386,941 + +0.7 2,387,163 - +1.2 2,387,253 + +1.0 2,387,254 - +2.4 2,387,463 + +1.4
Or see this region's nucleotide sequence