Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1987 and Pf1N1B4_1988 are separated by 11 nucleotides Pf1N1B4_1988 and Pf1N1B4_1989 overlap by 1 nucleotides Pf1N1B4_1989 and Pf1N1B4_1990 overlap by 4 nucleotides Pf1N1B4_1990 and Pf1N1B4_1991 are separated by 86 nucleotides
Pf1N1B4_1987 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 2,272,792 to 2,273,667
_1987
Pf1N1B4_1988 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 2,273,679 to 2,274,545
_1988
Pf1N1B4_1989 - Zinc ABC transporter, ATP-binding protein ZnuC, at 2,274,545 to 2,275,213
_1989
Pf1N1B4_1990 - GTP cyclohydrolase I (EC 3.5.4.16) type 2, at 2,275,210 to 2,276,106
_1990
Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401
_1991
Position (kb)
2274
2275
2276 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2273.597 kb on - strand at 2273.657 kb on - strand at 2273.674 kb on - strand at 2273.674 kb on - strand at 2273.749 kb on + strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.797 kb on + strand, within Pf1N1B4_1988 at 2273.798 kb on - strand at 2273.800 kb on + strand, within Pf1N1B4_1988 at 2273.800 kb on + strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.989 kb on - strand, within Pf1N1B4_1988 at 2273.989 kb on - strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.159 kb on + strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.426 kb on + strand, within Pf1N1B4_1988 at 2274.427 kb on - strand, within Pf1N1B4_1988 at 2274.624 kb on - strand, within Pf1N1B4_1989 at 2275.485 kb on - strand, within Pf1N1B4_1990 at 2275.485 kb on - strand, within Pf1N1B4_1990 at 2275.673 kb on + strand, within Pf1N1B4_1990 at 2275.674 kb on - strand, within Pf1N1B4_1990 at 2275.792 kb on + strand, within Pf1N1B4_1990 at 2275.792 kb on + strand at 2275.792 kb on + strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.815 kb on - strand, within Pf1N1B4_1990 at 2275.919 kb on + strand, within Pf1N1B4_1990 at 2275.919 kb on + strand, within Pf1N1B4_1990 at 2275.920 kb on - strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 2,273,597 - -1.0 2,273,657 - +0.2 2,273,674 - +0.5 2,273,674 - +0.2 2,273,749 + +0.2 2,273,750 - -1.4 2,273,750 - -0.8 2,273,750 - +1.0 2,273,750 - -0.9 2,273,750 - -0.8 2,273,750 - +2.2 2,273,797 + Pf1N1B4_1988 0.14 -0.8 2,273,798 - +0.2 2,273,800 + Pf1N1B4_1988 0.14 +0.6 2,273,800 + Pf1N1B4_1988 0.14 +0.7 2,273,801 - Pf1N1B4_1988 0.14 -0.2 2,273,801 - Pf1N1B4_1988 0.14 -1.0 2,273,801 - Pf1N1B4_1988 0.14 +0.4 2,273,801 - Pf1N1B4_1988 0.14 +1.0 2,273,801 - Pf1N1B4_1988 0.14 +0.7 2,273,988 + Pf1N1B4_1988 0.36 -1.7 2,273,988 + Pf1N1B4_1988 0.36 +0.8 2,273,988 + Pf1N1B4_1988 0.36 +0.2 2,273,988 + Pf1N1B4_1988 0.36 -0.4 2,273,989 - Pf1N1B4_1988 0.36 +0.7 2,273,989 - Pf1N1B4_1988 0.36 -0.2 2,274,067 + Pf1N1B4_1988 0.45 -0.2 2,274,067 + Pf1N1B4_1988 0.45 -0.1 2,274,067 + Pf1N1B4_1988 0.45 -0.8 2,274,067 + Pf1N1B4_1988 0.45 -0.0 2,274,067 + Pf1N1B4_1988 0.45 +1.7 2,274,067 + Pf1N1B4_1988 0.45 -1.8 2,274,068 - Pf1N1B4_1988 0.45 +1.0 2,274,068 - Pf1N1B4_1988 0.45 -0.4 2,274,068 - Pf1N1B4_1988 0.45 -1.2 2,274,068 - Pf1N1B4_1988 0.45 +0.0 2,274,068 - Pf1N1B4_1988 0.45 +0.3 2,274,068 - Pf1N1B4_1988 0.45 +0.0 2,274,159 + Pf1N1B4_1988 0.55 -1.0 2,274,160 - Pf1N1B4_1988 0.55 +1.5 2,274,160 - Pf1N1B4_1988 0.55 -2.2 2,274,160 - Pf1N1B4_1988 0.55 +0.2 2,274,160 - +2.2 2,274,160 - Pf1N1B4_1988 0.55 +1.4 2,274,160 - Pf1N1B4_1988 0.55 -1.4 2,274,160 - Pf1N1B4_1988 0.55 -1.7 2,274,426 + Pf1N1B4_1988 0.86 -0.1 2,274,427 - Pf1N1B4_1988 0.86 -0.1 2,274,624 - Pf1N1B4_1989 0.12 +0.8 2,275,485 - Pf1N1B4_1990 0.31 -0.2 2,275,485 - Pf1N1B4_1990 0.31 +0.0 2,275,673 + Pf1N1B4_1990 0.52 +1.8 2,275,674 - Pf1N1B4_1990 0.52 +0.1 2,275,792 + Pf1N1B4_1990 0.65 +1.0 2,275,792 + +2.0 2,275,792 + Pf1N1B4_1990 0.65 +0.8 2,275,793 - Pf1N1B4_1990 0.65 -2.2 2,275,793 - Pf1N1B4_1990 0.65 +0.5 2,275,793 - Pf1N1B4_1990 0.65 -1.6 2,275,793 - Pf1N1B4_1990 0.65 +0.3 2,275,793 - Pf1N1B4_1990 0.65 -0.6 2,275,815 - Pf1N1B4_1990 0.67 +1.4 2,275,919 + Pf1N1B4_1990 0.79 +0.2 2,275,919 + Pf1N1B4_1990 0.79 +0.2 2,275,920 - Pf1N1B4_1990 0.79 +1.2 2,275,932 + Pf1N1B4_1990 0.80 +0.5 2,275,932 + Pf1N1B4_1990 0.80 +1.1 2,275,932 + Pf1N1B4_1990 0.80 -0.4 2,275,932 + Pf1N1B4_1990 0.80 +0.5 2,275,932 + Pf1N1B4_1990 0.80 -0.1 2,275,932 + Pf1N1B4_1990 0.80 -0.2 2,275,932 + Pf1N1B4_1990 0.80 -0.5 2,275,932 + Pf1N1B4_1990 0.80 -0.9 2,275,932 + Pf1N1B4_1990 0.80 -0.5 2,275,932 + Pf1N1B4_1990 0.80 -0.8 2,275,932 + Pf1N1B4_1990 0.80 +0.2 2,275,932 + Pf1N1B4_1990 0.80 -0.8 2,275,932 + Pf1N1B4_1990 0.80 +0.6 2,275,932 + Pf1N1B4_1990 0.80 +0.2 2,275,932 + Pf1N1B4_1990 0.80 +1.6 2,275,933 - Pf1N1B4_1990 0.81 -0.5 2,275,933 - Pf1N1B4_1990 0.81 +2.5 2,275,933 - Pf1N1B4_1990 0.81 -0.1 2,275,933 - Pf1N1B4_1990 0.81 -0.4
Or see this region's nucleotide sequence