Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1988

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1986 and Pf1N1B4_1987 overlap by 4 nucleotidesPf1N1B4_1987 and Pf1N1B4_1988 are separated by 11 nucleotidesPf1N1B4_1988 and Pf1N1B4_1989 overlap by 1 nucleotidesPf1N1B4_1989 and Pf1N1B4_1990 overlap by 4 nucleotides Pf1N1B4_1986 - Carbonic anhydrase, gamma class (EC 4.2.1.1), at 2,272,193 to 2,272,795 _1986 Pf1N1B4_1987 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 2,272,792 to 2,273,667 _1987 Pf1N1B4_1988 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 2,273,679 to 2,274,545 _1988 Pf1N1B4_1989 - Zinc ABC transporter, ATP-binding protein ZnuC, at 2,274,545 to 2,275,213 _1989 Pf1N1B4_1990 - GTP cyclohydrolase I (EC 3.5.4.16) type 2, at 2,275,210 to 2,276,106 _1990 Position (kb) 2273 2274 2275Strain fitness (log2 ratio) -2 -1 0 1 2at 2272.735 kb on + strandat 2272.735 kb on + strandat 2272.744 kb on - strandat 2272.744 kb on - strandat 2272.744 kb on - strandat 2272.814 kb on + strandat 2272.815 kb on - strandat 2272.815 kb on - strandat 2272.832 kb on + strandat 2272.832 kb on + strandat 2272.832 kb on + strandat 2272.833 kb on - strandat 2272.833 kb on - strandat 2273.130 kb on + strand, within Pf1N1B4_1987at 2273.130 kb on + strand, within Pf1N1B4_1987at 2273.180 kb on + strand, within Pf1N1B4_1987at 2273.180 kb on + strand, within Pf1N1B4_1987at 2273.181 kb on - strand, within Pf1N1B4_1987at 2273.181 kb on - strand, within Pf1N1B4_1987at 2273.181 kb on - strand, within Pf1N1B4_1987at 2273.195 kb on + strand, within Pf1N1B4_1987at 2273.196 kb on - strand, within Pf1N1B4_1987at 2273.273 kb on + strand, within Pf1N1B4_1987at 2273.273 kb on + strand, within Pf1N1B4_1987at 2273.273 kb on + strand, within Pf1N1B4_1987at 2273.273 kb on + strand, within Pf1N1B4_1987at 2273.273 kb on + strand, within Pf1N1B4_1987at 2273.273 kb on + strand, within Pf1N1B4_1987at 2273.273 kb on + strand, within Pf1N1B4_1987at 2273.274 kb on - strand, within Pf1N1B4_1987at 2273.274 kb on - strand, within Pf1N1B4_1987at 2273.274 kb on - strand, within Pf1N1B4_1987at 2273.274 kb on - strand, within Pf1N1B4_1987at 2273.274 kb on - strand, within Pf1N1B4_1987at 2273.274 kb on - strand, within Pf1N1B4_1987at 2273.432 kb on - strand, within Pf1N1B4_1987at 2273.495 kb on + strand, within Pf1N1B4_1987at 2273.495 kb on + strand, within Pf1N1B4_1987at 2273.496 kb on - strand, within Pf1N1B4_1987at 2273.496 kb on - strand, within Pf1N1B4_1987at 2273.496 kb on - strand, within Pf1N1B4_1987at 2273.496 kb on - strand, within Pf1N1B4_1987at 2273.500 kb on - strand, within Pf1N1B4_1987at 2273.500 kb on - strand, within Pf1N1B4_1987at 2273.500 kb on - strand, within Pf1N1B4_1987at 2273.541 kb on + strand, within Pf1N1B4_1987at 2273.541 kb on + strand, within Pf1N1B4_1987at 2273.597 kb on - strandat 2273.657 kb on - strandat 2273.674 kb on - strandat 2273.674 kb on - strandat 2273.749 kb on + strandat 2273.750 kb on - strandat 2273.750 kb on - strandat 2273.750 kb on - strandat 2273.750 kb on - strandat 2273.750 kb on - strandat 2273.750 kb on - strandat 2273.797 kb on + strand, within Pf1N1B4_1988at 2273.798 kb on - strandat 2273.800 kb on + strand, within Pf1N1B4_1988at 2273.800 kb on + strand, within Pf1N1B4_1988at 2273.801 kb on - strand, within Pf1N1B4_1988at 2273.801 kb on - strand, within Pf1N1B4_1988at 2273.801 kb on - strand, within Pf1N1B4_1988at 2273.801 kb on - strand, within Pf1N1B4_1988at 2273.801 kb on - strand, within Pf1N1B4_1988at 2273.988 kb on + strand, within Pf1N1B4_1988at 2273.988 kb on + strand, within Pf1N1B4_1988at 2273.988 kb on + strand, within Pf1N1B4_1988at 2273.988 kb on + strand, within Pf1N1B4_1988at 2273.989 kb on - strand, within Pf1N1B4_1988at 2273.989 kb on - strand, within Pf1N1B4_1988at 2274.067 kb on + strand, within Pf1N1B4_1988at 2274.067 kb on + strand, within Pf1N1B4_1988at 2274.067 kb on + strand, within Pf1N1B4_1988at 2274.067 kb on + strand, within Pf1N1B4_1988at 2274.067 kb on + strand, within Pf1N1B4_1988at 2274.067 kb on + strand, within Pf1N1B4_1988at 2274.068 kb on - strand, within Pf1N1B4_1988at 2274.068 kb on - strand, within Pf1N1B4_1988at 2274.068 kb on - strand, within Pf1N1B4_1988at 2274.068 kb on - strand, within Pf1N1B4_1988at 2274.068 kb on - strand, within Pf1N1B4_1988at 2274.068 kb on - strand, within Pf1N1B4_1988at 2274.159 kb on + strand, within Pf1N1B4_1988at 2274.160 kb on - strand, within Pf1N1B4_1988at 2274.160 kb on - strand, within Pf1N1B4_1988at 2274.160 kb on - strand, within Pf1N1B4_1988at 2274.160 kb on - strandat 2274.160 kb on - strand, within Pf1N1B4_1988at 2274.160 kb on - strand, within Pf1N1B4_1988at 2274.160 kb on - strand, within Pf1N1B4_1988at 2274.426 kb on + strand, within Pf1N1B4_1988at 2274.427 kb on - strand, within Pf1N1B4_1988at 2274.624 kb on - strand, within Pf1N1B4_1989at 2275.485 kb on - strand, within Pf1N1B4_1990at 2275.485 kb on - strand, within Pf1N1B4_1990

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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2,272,735 + +0.8
2,272,735 + -0.8
2,272,744 - -0.5
2,272,744 - +0.0
2,272,744 - -0.6
2,272,814 + -1.1
2,272,815 - -0.8
2,272,815 - +0.5
2,272,832 + +0.0
2,272,832 + +0.8
2,272,832 + +2.5
2,272,833 - -0.4
2,272,833 - -1.0
2,273,130 + Pf1N1B4_1987 0.39 -0.0
2,273,130 + Pf1N1B4_1987 0.39 +0.4
2,273,180 + Pf1N1B4_1987 0.44 +0.2
2,273,180 + Pf1N1B4_1987 0.44 -0.8
2,273,181 - Pf1N1B4_1987 0.44 +0.7
2,273,181 - Pf1N1B4_1987 0.44 -0.3
2,273,181 - Pf1N1B4_1987 0.44 +0.3
2,273,195 + Pf1N1B4_1987 0.46 -1.4
2,273,196 - Pf1N1B4_1987 0.46 -0.3
2,273,273 + Pf1N1B4_1987 0.55 +1.2
2,273,273 + Pf1N1B4_1987 0.55 +0.7
2,273,273 + Pf1N1B4_1987 0.55 +0.2
2,273,273 + Pf1N1B4_1987 0.55 -0.4
2,273,273 + Pf1N1B4_1987 0.55 +1.2
2,273,273 + Pf1N1B4_1987 0.55 -1.4
2,273,273 + Pf1N1B4_1987 0.55 +2.2
2,273,274 - Pf1N1B4_1987 0.55 +2.2
2,273,274 - Pf1N1B4_1987 0.55 -0.5
2,273,274 - Pf1N1B4_1987 0.55 -1.0
2,273,274 - Pf1N1B4_1987 0.55 +0.1
2,273,274 - Pf1N1B4_1987 0.55 +1.6
2,273,274 - Pf1N1B4_1987 0.55 +0.2
2,273,432 - Pf1N1B4_1987 0.73 -0.1
2,273,495 + Pf1N1B4_1987 0.80 +1.2
2,273,495 + Pf1N1B4_1987 0.80 -0.4
2,273,496 - Pf1N1B4_1987 0.80 +0.5
2,273,496 - Pf1N1B4_1987 0.80 +1.0
2,273,496 - Pf1N1B4_1987 0.80 +0.9
2,273,496 - Pf1N1B4_1987 0.80 -0.0
2,273,500 - Pf1N1B4_1987 0.81 +0.5
2,273,500 - Pf1N1B4_1987 0.81 +2.1
2,273,500 - Pf1N1B4_1987 0.81 -0.4
2,273,541 + Pf1N1B4_1987 0.86 +2.0
2,273,541 + Pf1N1B4_1987 0.86 +0.6
2,273,597 - -1.0
2,273,657 - +0.2
2,273,674 - +0.5
2,273,674 - +0.2
2,273,749 + +0.2
2,273,750 - -1.4
2,273,750 - -0.8
2,273,750 - +1.0
2,273,750 - -0.9
2,273,750 - -0.8
2,273,750 - +2.2
2,273,797 + Pf1N1B4_1988 0.14 -0.8
2,273,798 - +0.2
2,273,800 + Pf1N1B4_1988 0.14 +0.6
2,273,800 + Pf1N1B4_1988 0.14 +0.7
2,273,801 - Pf1N1B4_1988 0.14 -0.2
2,273,801 - Pf1N1B4_1988 0.14 -1.0
2,273,801 - Pf1N1B4_1988 0.14 +0.4
2,273,801 - Pf1N1B4_1988 0.14 +1.0
2,273,801 - Pf1N1B4_1988 0.14 +0.7
2,273,988 + Pf1N1B4_1988 0.36 -1.7
2,273,988 + Pf1N1B4_1988 0.36 +0.8
2,273,988 + Pf1N1B4_1988 0.36 +0.2
2,273,988 + Pf1N1B4_1988 0.36 -0.4
2,273,989 - Pf1N1B4_1988 0.36 +0.7
2,273,989 - Pf1N1B4_1988 0.36 -0.2
2,274,067 + Pf1N1B4_1988 0.45 -0.2
2,274,067 + Pf1N1B4_1988 0.45 -0.1
2,274,067 + Pf1N1B4_1988 0.45 -0.8
2,274,067 + Pf1N1B4_1988 0.45 -0.0
2,274,067 + Pf1N1B4_1988 0.45 +1.7
2,274,067 + Pf1N1B4_1988 0.45 -1.8
2,274,068 - Pf1N1B4_1988 0.45 +1.0
2,274,068 - Pf1N1B4_1988 0.45 -0.4
2,274,068 - Pf1N1B4_1988 0.45 -1.2
2,274,068 - Pf1N1B4_1988 0.45 +0.0
2,274,068 - Pf1N1B4_1988 0.45 +0.3
2,274,068 - Pf1N1B4_1988 0.45 +0.0
2,274,159 + Pf1N1B4_1988 0.55 -1.0
2,274,160 - Pf1N1B4_1988 0.55 +1.5
2,274,160 - Pf1N1B4_1988 0.55 -2.2
2,274,160 - Pf1N1B4_1988 0.55 +0.2
2,274,160 - +2.2
2,274,160 - Pf1N1B4_1988 0.55 +1.4
2,274,160 - Pf1N1B4_1988 0.55 -1.4
2,274,160 - Pf1N1B4_1988 0.55 -1.7
2,274,426 + Pf1N1B4_1988 0.86 -0.1
2,274,427 - Pf1N1B4_1988 0.86 -0.1
2,274,624 - Pf1N1B4_1989 0.12 +0.8
2,275,485 - Pf1N1B4_1990 0.31 -0.2
2,275,485 - Pf1N1B4_1990 0.31 +0.0

Or see this region's nucleotide sequence