Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1985 and Pf1N1B4_1986 overlap by 29 nucleotides Pf1N1B4_1986 and Pf1N1B4_1987 overlap by 4 nucleotides Pf1N1B4_1987 and Pf1N1B4_1988 are separated by 11 nucleotides Pf1N1B4_1988 and Pf1N1B4_1989 overlap by 1 nucleotides
Pf1N1B4_1985 - Dihydroorotase (EC 3.5.2.3), at 2,270,896 to 2,272,221
_1985
Pf1N1B4_1986 - Carbonic anhydrase, gamma class (EC 4.2.1.1), at 2,272,193 to 2,272,795
_1986
Pf1N1B4_1987 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 2,272,792 to 2,273,667
_1987
Pf1N1B4_1988 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 2,273,679 to 2,274,545
_1988
Pf1N1B4_1989 - Zinc ABC transporter, ATP-binding protein ZnuC, at 2,274,545 to 2,275,213
_1989
Position (kb)
2272
2273
2274 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2271.875 kb on + strand, within Pf1N1B4_1985 at 2271.876 kb on - strand, within Pf1N1B4_1985 at 2271.876 kb on - strand, within Pf1N1B4_1985 at 2272.009 kb on - strand, within Pf1N1B4_1985 at 2272.260 kb on + strand, within Pf1N1B4_1986 at 2272.260 kb on + strand, within Pf1N1B4_1986 at 2272.261 kb on - strand, within Pf1N1B4_1986 at 2272.261 kb on - strand, within Pf1N1B4_1986 at 2272.383 kb on + strand, within Pf1N1B4_1986 at 2272.383 kb on + strand, within Pf1N1B4_1986 at 2272.383 kb on + strand, within Pf1N1B4_1986 at 2272.383 kb on + strand, within Pf1N1B4_1986 at 2272.383 kb on + strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.384 kb on - strand, within Pf1N1B4_1986 at 2272.487 kb on + strand, within Pf1N1B4_1986 at 2272.487 kb on + strand at 2272.496 kb on - strand, within Pf1N1B4_1986 at 2272.677 kb on + strand, within Pf1N1B4_1986 at 2272.677 kb on + strand, within Pf1N1B4_1986 at 2272.678 kb on - strand, within Pf1N1B4_1986 at 2272.735 kb on + strand at 2272.735 kb on + strand at 2272.744 kb on - strand at 2272.744 kb on - strand at 2272.744 kb on - strand at 2272.814 kb on + strand at 2272.815 kb on - strand at 2272.815 kb on - strand at 2272.832 kb on + strand at 2272.832 kb on + strand at 2272.832 kb on + strand at 2272.833 kb on - strand at 2272.833 kb on - strand at 2273.130 kb on + strand, within Pf1N1B4_1987 at 2273.130 kb on + strand, within Pf1N1B4_1987 at 2273.180 kb on + strand, within Pf1N1B4_1987 at 2273.180 kb on + strand, within Pf1N1B4_1987 at 2273.181 kb on - strand, within Pf1N1B4_1987 at 2273.181 kb on - strand, within Pf1N1B4_1987 at 2273.181 kb on - strand, within Pf1N1B4_1987 at 2273.195 kb on + strand, within Pf1N1B4_1987 at 2273.196 kb on - strand, within Pf1N1B4_1987 at 2273.273 kb on + strand, within Pf1N1B4_1987 at 2273.273 kb on + strand, within Pf1N1B4_1987 at 2273.273 kb on + strand, within Pf1N1B4_1987 at 2273.273 kb on + strand, within Pf1N1B4_1987 at 2273.273 kb on + strand, within Pf1N1B4_1987 at 2273.273 kb on + strand, within Pf1N1B4_1987 at 2273.273 kb on + strand, within Pf1N1B4_1987 at 2273.274 kb on - strand, within Pf1N1B4_1987 at 2273.274 kb on - strand, within Pf1N1B4_1987 at 2273.274 kb on - strand, within Pf1N1B4_1987 at 2273.274 kb on - strand, within Pf1N1B4_1987 at 2273.274 kb on - strand, within Pf1N1B4_1987 at 2273.274 kb on - strand, within Pf1N1B4_1987 at 2273.432 kb on - strand, within Pf1N1B4_1987 at 2273.495 kb on + strand, within Pf1N1B4_1987 at 2273.495 kb on + strand, within Pf1N1B4_1987 at 2273.496 kb on - strand, within Pf1N1B4_1987 at 2273.496 kb on - strand, within Pf1N1B4_1987 at 2273.496 kb on - strand, within Pf1N1B4_1987 at 2273.496 kb on - strand, within Pf1N1B4_1987 at 2273.500 kb on - strand, within Pf1N1B4_1987 at 2273.500 kb on - strand, within Pf1N1B4_1987 at 2273.500 kb on - strand, within Pf1N1B4_1987 at 2273.541 kb on + strand, within Pf1N1B4_1987 at 2273.541 kb on + strand, within Pf1N1B4_1987 at 2273.597 kb on - strand at 2273.657 kb on - strand at 2273.674 kb on - strand at 2273.674 kb on - strand at 2273.749 kb on + strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.750 kb on - strand at 2273.797 kb on + strand, within Pf1N1B4_1988 at 2273.798 kb on - strand at 2273.800 kb on + strand, within Pf1N1B4_1988 at 2273.800 kb on + strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.801 kb on - strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.988 kb on + strand, within Pf1N1B4_1988 at 2273.989 kb on - strand, within Pf1N1B4_1988 at 2273.989 kb on - strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.067 kb on + strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.068 kb on - strand, within Pf1N1B4_1988 at 2274.159 kb on + strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.160 kb on - strand, within Pf1N1B4_1988 at 2274.426 kb on + strand, within Pf1N1B4_1988 at 2274.427 kb on - strand, within Pf1N1B4_1988 at 2274.624 kb on - strand, within Pf1N1B4_1989
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 2,271,875 + Pf1N1B4_1985 0.74 +0.2 2,271,876 - Pf1N1B4_1985 0.74 +0.5 2,271,876 - Pf1N1B4_1985 0.74 -1.0 2,272,009 - Pf1N1B4_1985 0.84 +0.3 2,272,260 + Pf1N1B4_1986 0.11 -1.6 2,272,260 + Pf1N1B4_1986 0.11 +2.2 2,272,261 - Pf1N1B4_1986 0.11 -1.1 2,272,261 - Pf1N1B4_1986 0.11 -0.4 2,272,383 + Pf1N1B4_1986 0.32 -0.4 2,272,383 + Pf1N1B4_1986 0.32 +1.2 2,272,383 + Pf1N1B4_1986 0.32 +0.0 2,272,383 + Pf1N1B4_1986 0.32 +1.9 2,272,383 + Pf1N1B4_1986 0.32 -0.8 2,272,384 - Pf1N1B4_1986 0.32 +1.0 2,272,384 - Pf1N1B4_1986 0.32 -0.4 2,272,384 - Pf1N1B4_1986 0.32 -0.2 2,272,384 - Pf1N1B4_1986 0.32 +0.6 2,272,384 - Pf1N1B4_1986 0.32 -0.5 2,272,384 - Pf1N1B4_1986 0.32 +0.0 2,272,384 - Pf1N1B4_1986 0.32 +2.6 2,272,384 - Pf1N1B4_1986 0.32 +0.2 2,272,384 - Pf1N1B4_1986 0.32 +0.2 2,272,384 - Pf1N1B4_1986 0.32 -1.1 2,272,384 - +1.2 2,272,384 - Pf1N1B4_1986 0.32 +0.0 2,272,384 - Pf1N1B4_1986 0.32 -0.4 2,272,384 - Pf1N1B4_1986 0.32 -0.2 2,272,487 + Pf1N1B4_1986 0.49 -0.5 2,272,487 + +1.2 2,272,496 - Pf1N1B4_1986 0.50 +1.5 2,272,677 + Pf1N1B4_1986 0.80 +1.2 2,272,677 + Pf1N1B4_1986 0.80 -0.8 2,272,678 - Pf1N1B4_1986 0.80 +0.8 2,272,735 + +0.8 2,272,735 + -0.8 2,272,744 - -0.5 2,272,744 - +0.0 2,272,744 - -0.6 2,272,814 + -1.1 2,272,815 - -0.8 2,272,815 - +0.5 2,272,832 + +0.0 2,272,832 + +0.8 2,272,832 + +2.5 2,272,833 - -0.4 2,272,833 - -1.0 2,273,130 + Pf1N1B4_1987 0.39 -0.0 2,273,130 + Pf1N1B4_1987 0.39 +0.4 2,273,180 + Pf1N1B4_1987 0.44 +0.2 2,273,180 + Pf1N1B4_1987 0.44 -0.8 2,273,181 - Pf1N1B4_1987 0.44 +0.7 2,273,181 - Pf1N1B4_1987 0.44 -0.3 2,273,181 - Pf1N1B4_1987 0.44 +0.3 2,273,195 + Pf1N1B4_1987 0.46 -1.4 2,273,196 - Pf1N1B4_1987 0.46 -0.3 2,273,273 + Pf1N1B4_1987 0.55 +1.2 2,273,273 + Pf1N1B4_1987 0.55 +0.7 2,273,273 + Pf1N1B4_1987 0.55 +0.2 2,273,273 + Pf1N1B4_1987 0.55 -0.4 2,273,273 + Pf1N1B4_1987 0.55 +1.2 2,273,273 + Pf1N1B4_1987 0.55 -1.4 2,273,273 + Pf1N1B4_1987 0.55 +2.2 2,273,274 - Pf1N1B4_1987 0.55 +2.2 2,273,274 - Pf1N1B4_1987 0.55 -0.5 2,273,274 - Pf1N1B4_1987 0.55 -1.0 2,273,274 - Pf1N1B4_1987 0.55 +0.1 2,273,274 - Pf1N1B4_1987 0.55 +1.6 2,273,274 - Pf1N1B4_1987 0.55 +0.2 2,273,432 - Pf1N1B4_1987 0.73 -0.1 2,273,495 + Pf1N1B4_1987 0.80 +1.2 2,273,495 + Pf1N1B4_1987 0.80 -0.4 2,273,496 - Pf1N1B4_1987 0.80 +0.5 2,273,496 - Pf1N1B4_1987 0.80 +1.0 2,273,496 - Pf1N1B4_1987 0.80 +0.9 2,273,496 - Pf1N1B4_1987 0.80 -0.0 2,273,500 - Pf1N1B4_1987 0.81 +0.5 2,273,500 - Pf1N1B4_1987 0.81 +2.1 2,273,500 - Pf1N1B4_1987 0.81 -0.4 2,273,541 + Pf1N1B4_1987 0.86 +2.0 2,273,541 + Pf1N1B4_1987 0.86 +0.6 2,273,597 - -1.0 2,273,657 - +0.2 2,273,674 - +0.5 2,273,674 - +0.2 2,273,749 + +0.2 2,273,750 - -1.4 2,273,750 - -0.8 2,273,750 - +1.0 2,273,750 - -0.9 2,273,750 - -0.8 2,273,750 - +2.2 2,273,797 + Pf1N1B4_1988 0.14 -0.8 2,273,798 - +0.2 2,273,800 + Pf1N1B4_1988 0.14 +0.6 2,273,800 + Pf1N1B4_1988 0.14 +0.7 2,273,801 - Pf1N1B4_1988 0.14 -0.2 2,273,801 - Pf1N1B4_1988 0.14 -1.0 2,273,801 - Pf1N1B4_1988 0.14 +0.4 2,273,801 - Pf1N1B4_1988 0.14 +1.0 2,273,801 - Pf1N1B4_1988 0.14 +0.7 2,273,988 + Pf1N1B4_1988 0.36 -1.7 2,273,988 + Pf1N1B4_1988 0.36 +0.8 2,273,988 + Pf1N1B4_1988 0.36 +0.2 2,273,988 + Pf1N1B4_1988 0.36 -0.4 2,273,989 - Pf1N1B4_1988 0.36 +0.7 2,273,989 - Pf1N1B4_1988 0.36 -0.2 2,274,067 + Pf1N1B4_1988 0.45 -0.2 2,274,067 + Pf1N1B4_1988 0.45 -0.1 2,274,067 + Pf1N1B4_1988 0.45 -0.8 2,274,067 + Pf1N1B4_1988 0.45 -0.0 2,274,067 + Pf1N1B4_1988 0.45 +1.7 2,274,067 + Pf1N1B4_1988 0.45 -1.8 2,274,068 - Pf1N1B4_1988 0.45 +1.0 2,274,068 - Pf1N1B4_1988 0.45 -0.4 2,274,068 - Pf1N1B4_1988 0.45 -1.2 2,274,068 - Pf1N1B4_1988 0.45 +0.0 2,274,068 - Pf1N1B4_1988 0.45 +0.3 2,274,068 - Pf1N1B4_1988 0.45 +0.0 2,274,159 + Pf1N1B4_1988 0.55 -1.0 2,274,160 - Pf1N1B4_1988 0.55 +1.5 2,274,160 - Pf1N1B4_1988 0.55 -2.2 2,274,160 - Pf1N1B4_1988 0.55 +0.2 2,274,160 - +2.2 2,274,160 - Pf1N1B4_1988 0.55 +1.4 2,274,160 - Pf1N1B4_1988 0.55 -1.4 2,274,160 - Pf1N1B4_1988 0.55 -1.7 2,274,426 + Pf1N1B4_1988 0.86 -0.1 2,274,427 - Pf1N1B4_1988 0.86 -0.1 2,274,624 - Pf1N1B4_1989 0.12 +0.8
Or see this region's nucleotide sequence