Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1720 and Pf1N1B4_1721 are separated by 111 nucleotides Pf1N1B4_1721 and Pf1N1B4_1722 are separated by 111 nucleotides Pf1N1B4_1722 and Pf1N1B4_1723 are separated by 85 nucleotides Pf1N1B4_1723 and Pf1N1B4_1724 are separated by 90 nucleotides
Pf1N1B4_1720 - Helix-turn-helix motif, at 1,968,426 to 1,968,674
_1720
Pf1N1B4_1721 - DNA-binding heavy metal response regulator, at 1,968,786 to 1,969,151
_1721
Pf1N1B4_1722 - transcriptional regulator, Crp/Fnr family, at 1,969,263 to 1,969,949
_1722
Pf1N1B4_1723 - FIG028593: membrane protein, at 1,970,035 to 1,970,553
_1723
Pf1N1B4_1724 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 1,970,644 to 1,971,633
_1724
Position (kb)
1969
1970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1968.267 kb on + strand at 1968.267 kb on + strand at 1968.267 kb on + strand at 1968.268 kb on - strand at 1968.268 kb on - strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.335 kb on - strand at 1968.335 kb on - strand at 1968.335 kb on - strand at 1968.441 kb on - strand at 1968.441 kb on - strand at 1968.693 kb on - strand at 1968.715 kb on - strand at 1968.724 kb on - strand at 1968.724 kb on - strand at 1968.759 kb on + strand at 1968.760 kb on - strand at 1968.760 kb on - strand at 1968.760 kb on - strand at 1969.071 kb on + strand, within Pf1N1B4_1721 at 1969.072 kb on - strand, within Pf1N1B4_1721 at 1969.072 kb on - strand, within Pf1N1B4_1721 at 1969.079 kb on - strand, within Pf1N1B4_1721 at 1969.079 kb on - strand, within Pf1N1B4_1721 at 1969.079 kb on - strand, within Pf1N1B4_1721 at 1969.138 kb on - strand at 1969.182 kb on - strand at 1969.210 kb on + strand at 1969.305 kb on - strand at 1969.318 kb on + strand at 1969.319 kb on - strand at 1969.319 kb on - strand at 1969.323 kb on + strand at 1969.324 kb on - strand at 1969.324 kb on - strand at 1969.324 kb on - strand at 1969.580 kb on - strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.655 kb on - strand, within Pf1N1B4_1722 at 1969.767 kb on - strand, within Pf1N1B4_1722 at 1969.767 kb on - strand, within Pf1N1B4_1722 at 1969.792 kb on + strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.824 kb on + strand, within Pf1N1B4_1722 at 1969.825 kb on - strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.875 kb on - strand, within Pf1N1B4_1722 at 1969.882 kb on - strand at 1969.893 kb on + strand at 1969.893 kb on + strand at 1969.894 kb on - strand at 1969.894 kb on - strand at 1970.019 kb on + strand at 1970.020 kb on - strand at 1970.020 kb on - strand at 1970.020 kb on - strand at 1970.030 kb on + strand at 1970.030 kb on + strand at 1970.031 kb on - strand at 1970.031 kb on - strand at 1970.094 kb on + strand, within Pf1N1B4_1723 at 1970.095 kb on - strand, within Pf1N1B4_1723 at 1970.129 kb on + strand, within Pf1N1B4_1723 at 1970.129 kb on + strand, within Pf1N1B4_1723 at 1970.130 kb on - strand, within Pf1N1B4_1723 at 1970.221 kb on + strand, within Pf1N1B4_1723 at 1970.222 kb on - strand, within Pf1N1B4_1723 at 1970.222 kb on - strand, within Pf1N1B4_1723 at 1970.294 kb on + strand, within Pf1N1B4_1723 at 1970.294 kb on + strand, within Pf1N1B4_1723 at 1970.310 kb on + strand, within Pf1N1B4_1723 at 1970.310 kb on + strand, within Pf1N1B4_1723 at 1970.310 kb on + strand, within Pf1N1B4_1723 at 1970.311 kb on - strand, within Pf1N1B4_1723 at 1970.311 kb on - strand, within Pf1N1B4_1723 at 1970.311 kb on - strand, within Pf1N1B4_1723 at 1970.380 kb on + strand, within Pf1N1B4_1723 at 1970.380 kb on + strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.381 kb on - strand, within Pf1N1B4_1723 at 1970.383 kb on + strand, within Pf1N1B4_1723 at 1970.383 kb on + strand, within Pf1N1B4_1723 at 1970.551 kb on + strand at 1970.645 kb on + strand at 1970.645 kb on + strand at 1970.645 kb on + strand at 1970.646 kb on - strand at 1970.686 kb on + strand at 1970.687 kb on - strand at 1970.809 kb on - strand, within Pf1N1B4_1724 at 1970.809 kb on - strand, within Pf1N1B4_1724 at 1970.933 kb on + strand, within Pf1N1B4_1724
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,968,267 + -1.6 1,968,267 + +0.8 1,968,267 + -2.4 1,968,268 - +0.6 1,968,268 - -0.4 1,968,330 + +2.2 1,968,330 + -0.8 1,968,330 + -1.9 1,968,330 + -0.2 1,968,330 + -1.8 1,968,331 - +0.4 1,968,331 - -0.1 1,968,331 - +0.2 1,968,331 - -0.8 1,968,331 - +0.8 1,968,335 - +0.3 1,968,335 - -0.8 1,968,335 - +0.4 1,968,441 - -0.4 1,968,441 - +0.2 1,968,693 - -0.1 1,968,715 - -0.3 1,968,724 - -0.0 1,968,724 - +0.0 1,968,759 + +2.2 1,968,760 - +0.6 1,968,760 - -2.5 1,968,760 - +0.8 1,969,071 + Pf1N1B4_1721 0.78 +0.6 1,969,072 - Pf1N1B4_1721 0.78 +0.5 1,969,072 - Pf1N1B4_1721 0.78 -1.2 1,969,079 - Pf1N1B4_1721 0.80 +0.9 1,969,079 - Pf1N1B4_1721 0.80 +0.2 1,969,079 - Pf1N1B4_1721 0.80 +0.5 1,969,138 - -0.3 1,969,182 - +0.4 1,969,210 + +0.3 1,969,305 - -0.8 1,969,318 + -0.7 1,969,319 - -0.2 1,969,319 - -0.4 1,969,323 + -0.4 1,969,324 - -0.1 1,969,324 - +0.8 1,969,324 - -0.9 1,969,580 - Pf1N1B4_1722 0.46 +0.4 1,969,654 + Pf1N1B4_1722 0.57 -0.9 1,969,654 + Pf1N1B4_1722 0.57 -0.1 1,969,654 + Pf1N1B4_1722 0.57 +0.0 1,969,654 + Pf1N1B4_1722 0.57 +0.3 1,969,654 + Pf1N1B4_1722 0.57 -0.1 1,969,655 - Pf1N1B4_1722 0.57 +0.9 1,969,767 - Pf1N1B4_1722 0.73 +0.5 1,969,767 - Pf1N1B4_1722 0.73 -0.0 1,969,792 + Pf1N1B4_1722 0.77 +0.9 1,969,793 - Pf1N1B4_1722 0.77 +0.8 1,969,793 - Pf1N1B4_1722 0.77 +0.3 1,969,793 - Pf1N1B4_1722 0.77 +0.8 1,969,793 - Pf1N1B4_1722 0.77 -1.0 1,969,824 + Pf1N1B4_1722 0.82 -0.2 1,969,825 - Pf1N1B4_1722 0.82 +0.8 1,969,874 + Pf1N1B4_1722 0.89 -0.4 1,969,874 + Pf1N1B4_1722 0.89 -0.6 1,969,874 + Pf1N1B4_1722 0.89 -0.1 1,969,875 - Pf1N1B4_1722 0.89 -0.2 1,969,882 - -0.9 1,969,893 + -0.3 1,969,893 + -0.7 1,969,894 - -0.4 1,969,894 - +1.2 1,970,019 + -1.1 1,970,020 - -0.3 1,970,020 - -3.4 1,970,020 - -1.2 1,970,030 + +0.9 1,970,030 + -1.6 1,970,031 - +0.4 1,970,031 - +0.6 1,970,094 + Pf1N1B4_1723 0.11 -1.4 1,970,095 - Pf1N1B4_1723 0.12 +0.3 1,970,129 + Pf1N1B4_1723 0.18 -0.7 1,970,129 + Pf1N1B4_1723 0.18 +0.2 1,970,130 - Pf1N1B4_1723 0.18 +1.9 1,970,221 + Pf1N1B4_1723 0.36 -0.8 1,970,222 - Pf1N1B4_1723 0.36 -0.6 1,970,222 - Pf1N1B4_1723 0.36 -1.2 1,970,294 + Pf1N1B4_1723 0.50 +0.8 1,970,294 + Pf1N1B4_1723 0.50 -0.1 1,970,310 + Pf1N1B4_1723 0.53 -0.4 1,970,310 + Pf1N1B4_1723 0.53 -1.4 1,970,310 + Pf1N1B4_1723 0.53 +1.2 1,970,311 - Pf1N1B4_1723 0.53 +0.3 1,970,311 - Pf1N1B4_1723 0.53 +3.8 1,970,311 - Pf1N1B4_1723 0.53 -0.4 1,970,380 + Pf1N1B4_1723 0.66 +0.9 1,970,380 + Pf1N1B4_1723 0.66 -0.2 1,970,381 - Pf1N1B4_1723 0.67 +0.2 1,970,381 - Pf1N1B4_1723 0.67 -0.4 1,970,381 - Pf1N1B4_1723 0.67 -1.2 1,970,381 - Pf1N1B4_1723 0.67 +1.4 1,970,381 - Pf1N1B4_1723 0.67 +0.5 1,970,383 + Pf1N1B4_1723 0.67 +0.7 1,970,383 + Pf1N1B4_1723 0.67 -1.0 1,970,551 + -1.5 1,970,645 + +0.6 1,970,645 + +0.4 1,970,645 + -0.6 1,970,646 - -0.1 1,970,686 + -0.6 1,970,687 - -0.0 1,970,809 - Pf1N1B4_1724 0.17 +2.2 1,970,809 - Pf1N1B4_1724 0.17 -0.1 1,970,933 + Pf1N1B4_1724 0.29 +0.6
Or see this region's nucleotide sequence