Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1681 and Pf1N1B4_1682 are separated by 12 nucleotides Pf1N1B4_1682 and Pf1N1B4_1683 are separated by 72 nucleotides Pf1N1B4_1683 and Pf1N1B4_1684 are separated by 123 nucleotides
Pf1N1B4_1681 - Peptidase M50, at 1,927,320 to 1,929,416
_1681
Pf1N1B4_1682 - Acetyltransferase, GNAT family (EC 2.3.1.-), at 1,929,429 to 1,929,926
_1682
Pf1N1B4_1683 - Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17), at 1,929,999 to 1,930,841
_1683
Pf1N1B4_1684 - Oxidoreductase, aldo/keto reductase family, at 1,930,965 to 1,932,002
_1684
Position (kb)
1929
1930
1931 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1929.051 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand, within Pf1N1B4_1681 at 1929.174 kb on + strand at 1929.175 kb on - strand, within Pf1N1B4_1681 at 1929.175 kb on - strand, within Pf1N1B4_1681 at 1929.175 kb on - strand, within Pf1N1B4_1681 at 1929.175 kb on - strand at 1929.334 kb on + strand at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.574 kb on + strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.575 kb on - strand, within Pf1N1B4_1682 at 1929.639 kb on + strand at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.639 kb on + strand, within Pf1N1B4_1682 at 1929.640 kb on - strand, within Pf1N1B4_1682 at 1929.640 kb on - strand, within Pf1N1B4_1682 at 1929.714 kb on + strand, within Pf1N1B4_1682 at 1929.714 kb on + strand, within Pf1N1B4_1682 at 1929.714 kb on + strand, within Pf1N1B4_1682 at 1929.715 kb on - strand, within Pf1N1B4_1682 at 1929.715 kb on - strand, within Pf1N1B4_1682 at 1929.776 kb on - strand, within Pf1N1B4_1682 at 1929.852 kb on + strand, within Pf1N1B4_1682 at 1929.852 kb on + strand, within Pf1N1B4_1682 at 1929.852 kb on + strand, within Pf1N1B4_1682 at 1929.853 kb on - strand, within Pf1N1B4_1682 at 1929.913 kb on - strand at 1930.004 kb on + strand at 1930.182 kb on + strand, within Pf1N1B4_1683 at 1930.388 kb on + strand, within Pf1N1B4_1683 at 1930.389 kb on - strand, within Pf1N1B4_1683 at 1930.493 kb on + strand, within Pf1N1B4_1683 at 1930.967 kb on - strand at 1930.967 kb on - strand at 1931.298 kb on + strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.299 kb on - strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.302 kb on + strand, within Pf1N1B4_1684 at 1931.303 kb on - strand, within Pf1N1B4_1684 at 1931.413 kb on + strand, within Pf1N1B4_1684 at 1931.414 kb on - strand, within Pf1N1B4_1684 at 1931.414 kb on - strand, within Pf1N1B4_1684 at 1931.414 kb on - strand, within Pf1N1B4_1684 at 1931.440 kb on - strand at 1931.440 kb on - strand, within Pf1N1B4_1684 at 1931.440 kb on - strand, within Pf1N1B4_1684 at 1931.491 kb on + strand, within Pf1N1B4_1684 at 1931.502 kb on - strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.509 kb on + strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.510 kb on - strand, within Pf1N1B4_1684 at 1931.593 kb on + strand, within Pf1N1B4_1684 at 1931.594 kb on - strand, within Pf1N1B4_1684 at 1931.604 kb on + strand, within Pf1N1B4_1684 at 1931.604 kb on + strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.605 kb on - strand, within Pf1N1B4_1684 at 1931.608 kb on + strand, within Pf1N1B4_1684 at 1931.628 kb on + strand, within Pf1N1B4_1684 at 1931.628 kb on + strand, within Pf1N1B4_1684 at 1931.628 kb on + strand, within Pf1N1B4_1684 at 1931.629 kb on - strand, within Pf1N1B4_1684 at 1931.629 kb on - strand, within Pf1N1B4_1684 at 1931.629 kb on - strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.656 kb on + strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.657 kb on - strand, within Pf1N1B4_1684 at 1931.807 kb on - strand, within Pf1N1B4_1684 at 1931.807 kb on - strand, within Pf1N1B4_1684
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,929,051 + Pf1N1B4_1681 0.83 -0.2 1,929,174 + Pf1N1B4_1681 0.88 +1.1 1,929,174 + Pf1N1B4_1681 0.88 +0.8 1,929,174 + Pf1N1B4_1681 0.88 +1.1 1,929,174 + Pf1N1B4_1681 0.88 -0.1 1,929,174 + Pf1N1B4_1681 0.88 -1.2 1,929,174 + +1.7 1,929,175 - Pf1N1B4_1681 0.88 -1.2 1,929,175 - Pf1N1B4_1681 0.88 -0.1 1,929,175 - Pf1N1B4_1681 0.88 -0.4 1,929,175 - -0.4 1,929,334 + -0.3 1,929,574 + Pf1N1B4_1682 0.29 +0.7 1,929,574 + Pf1N1B4_1682 0.29 -1.3 1,929,574 + -0.4 1,929,574 + Pf1N1B4_1682 0.29 -0.6 1,929,574 + Pf1N1B4_1682 0.29 +1.7 1,929,574 + Pf1N1B4_1682 0.29 +1.5 1,929,574 + Pf1N1B4_1682 0.29 -1.8 1,929,575 - Pf1N1B4_1682 0.29 -0.9 1,929,575 - Pf1N1B4_1682 0.29 -0.3 1,929,575 - Pf1N1B4_1682 0.29 -0.2 1,929,575 - +1.1 1,929,575 - Pf1N1B4_1682 0.29 +2.1 1,929,575 - Pf1N1B4_1682 0.29 +0.9 1,929,575 - Pf1N1B4_1682 0.29 -0.0 1,929,575 - Pf1N1B4_1682 0.29 +0.3 1,929,639 + +0.1 1,929,639 + Pf1N1B4_1682 0.42 -1.2 1,929,639 + Pf1N1B4_1682 0.42 -0.2 1,929,639 + Pf1N1B4_1682 0.42 -0.7 1,929,639 + Pf1N1B4_1682 0.42 +0.2 1,929,639 + Pf1N1B4_1682 0.42 -0.3 1,929,639 + Pf1N1B4_1682 0.42 +1.4 1,929,639 + Pf1N1B4_1682 0.42 -0.7 1,929,640 - Pf1N1B4_1682 0.42 +2.1 1,929,640 - Pf1N1B4_1682 0.42 -1.6 1,929,714 + Pf1N1B4_1682 0.57 +0.1 1,929,714 + Pf1N1B4_1682 0.57 -1.7 1,929,714 + Pf1N1B4_1682 0.57 -0.6 1,929,715 - Pf1N1B4_1682 0.57 -0.4 1,929,715 - Pf1N1B4_1682 0.57 +0.1 1,929,776 - Pf1N1B4_1682 0.70 +1.5 1,929,852 + Pf1N1B4_1682 0.85 -0.4 1,929,852 + Pf1N1B4_1682 0.85 -1.4 1,929,852 + Pf1N1B4_1682 0.85 -0.7 1,929,853 - Pf1N1B4_1682 0.85 -0.1 1,929,913 - +1.1 1,930,004 + +0.6 1,930,182 + Pf1N1B4_1683 0.22 -0.2 1,930,388 + Pf1N1B4_1683 0.46 -0.9 1,930,389 - Pf1N1B4_1683 0.46 -0.5 1,930,493 + Pf1N1B4_1683 0.59 -0.2 1,930,967 - -0.3 1,930,967 - -0.0 1,931,298 + Pf1N1B4_1684 0.32 +1.0 1,931,299 - Pf1N1B4_1684 0.32 -0.9 1,931,299 - Pf1N1B4_1684 0.32 -0.3 1,931,299 - Pf1N1B4_1684 0.32 -0.2 1,931,299 - Pf1N1B4_1684 0.32 +0.3 1,931,299 - Pf1N1B4_1684 0.32 +1.6 1,931,299 - Pf1N1B4_1684 0.32 +0.1 1,931,299 - Pf1N1B4_1684 0.32 -0.6 1,931,302 + Pf1N1B4_1684 0.32 -1.0 1,931,302 + Pf1N1B4_1684 0.32 -1.2 1,931,302 + Pf1N1B4_1684 0.32 -0.7 1,931,302 + Pf1N1B4_1684 0.32 +0.7 1,931,302 + Pf1N1B4_1684 0.32 +0.7 1,931,303 - Pf1N1B4_1684 0.33 +0.1 1,931,413 + Pf1N1B4_1684 0.43 -0.4 1,931,414 - Pf1N1B4_1684 0.43 -0.2 1,931,414 - Pf1N1B4_1684 0.43 -0.9 1,931,414 - Pf1N1B4_1684 0.43 +1.1 1,931,440 - +0.4 1,931,440 - Pf1N1B4_1684 0.46 +2.1 1,931,440 - Pf1N1B4_1684 0.46 -0.7 1,931,491 + Pf1N1B4_1684 0.51 +1.1 1,931,502 - Pf1N1B4_1684 0.52 +0.6 1,931,509 + Pf1N1B4_1684 0.52 +0.7 1,931,509 + Pf1N1B4_1684 0.52 +1.6 1,931,509 + Pf1N1B4_1684 0.52 +0.1 1,931,509 + Pf1N1B4_1684 0.52 +0.1 1,931,509 + Pf1N1B4_1684 0.52 -0.6 1,931,509 + Pf1N1B4_1684 0.52 -1.3 1,931,509 + Pf1N1B4_1684 0.52 +0.1 1,931,509 + Pf1N1B4_1684 0.52 +0.1 1,931,510 - Pf1N1B4_1684 0.53 +0.1 1,931,510 - Pf1N1B4_1684 0.53 -1.4 1,931,510 - Pf1N1B4_1684 0.53 +1.3 1,931,510 - Pf1N1B4_1684 0.53 -0.5 1,931,593 + Pf1N1B4_1684 0.61 +0.7 1,931,594 - Pf1N1B4_1684 0.61 -0.2 1,931,604 + Pf1N1B4_1684 0.62 -0.9 1,931,604 + Pf1N1B4_1684 0.62 +0.6 1,931,605 - Pf1N1B4_1684 0.62 +0.1 1,931,605 - Pf1N1B4_1684 0.62 +1.1 1,931,605 - Pf1N1B4_1684 0.62 -2.2 1,931,605 - Pf1N1B4_1684 0.62 -0.4 1,931,608 + Pf1N1B4_1684 0.62 +1.0 1,931,628 + Pf1N1B4_1684 0.64 +0.4 1,931,628 + Pf1N1B4_1684 0.64 +0.6 1,931,628 + Pf1N1B4_1684 0.64 -0.6 1,931,629 - Pf1N1B4_1684 0.64 -0.0 1,931,629 - Pf1N1B4_1684 0.64 +0.9 1,931,629 - Pf1N1B4_1684 0.64 -0.7 1,931,656 + Pf1N1B4_1684 0.67 +0.1 1,931,656 + Pf1N1B4_1684 0.67 +1.1 1,931,656 + Pf1N1B4_1684 0.67 +0.1 1,931,656 + Pf1N1B4_1684 0.67 -1.7 1,931,657 - Pf1N1B4_1684 0.67 +1.6 1,931,657 - Pf1N1B4_1684 0.67 -0.1 1,931,657 - Pf1N1B4_1684 0.67 -2.2 1,931,657 - Pf1N1B4_1684 0.67 -0.5 1,931,807 - Pf1N1B4_1684 0.81 -0.8 1,931,807 - Pf1N1B4_1684 0.81 -0.9
Or see this region's nucleotide sequence