Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1543 and Pf1N1B4_1544 are separated by 215 nucleotides Pf1N1B4_1544 and Pf1N1B4_1545 are separated by 251 nucleotides
Pf1N1B4_1543 - Type IV pilus biogenesis protein PilM, at 1,778,680 to 1,779,744
_1543
Pf1N1B4_1544 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 1,779,960 to 1,782,407
_1544
Pf1N1B4_1545 - NADP-dependent malic enzyme (EC 1.1.1.40), at 1,782,659 to 1,783,927
_1545
Position (kb)
1779
1780
1781
1782
1783 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1778.979 kb on - strand, within Pf1N1B4_1543 at 1779.038 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.095 kb on + strand, within Pf1N1B4_1543 at 1779.096 kb on - strand, within Pf1N1B4_1543 at 1779.096 kb on - strand, within Pf1N1B4_1543 at 1779.227 kb on + strand, within Pf1N1B4_1543 at 1779.228 kb on - strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.404 kb on + strand, within Pf1N1B4_1543 at 1779.405 kb on - strand, within Pf1N1B4_1543 at 1779.405 kb on - strand, within Pf1N1B4_1543 at 1779.585 kb on + strand, within Pf1N1B4_1543 at 1779.629 kb on + strand, within Pf1N1B4_1543 at 1779.630 kb on - strand, within Pf1N1B4_1543 at 1779.664 kb on + strand at 1779.664 kb on + strand at 1779.665 kb on - strand at 1779.756 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.854 kb on + strand at 1779.856 kb on + strand at 1779.856 kb on + strand at 1779.856 kb on + strand at 1779.856 kb on + strand at 1779.906 kb on + strand at 1779.927 kb on + strand at 1780.085 kb on + strand at 1780.210 kb on + strand at 1780.301 kb on + strand, within Pf1N1B4_1544 at 1780.449 kb on + strand at 1780.464 kb on + strand, within Pf1N1B4_1544 at 1780.509 kb on + strand, within Pf1N1B4_1544 at 1780.509 kb on + strand, within Pf1N1B4_1544 at 1780.651 kb on - strand, within Pf1N1B4_1544 at 1780.651 kb on - strand, within Pf1N1B4_1544 at 1780.651 kb on - strand, within Pf1N1B4_1544 at 1780.652 kb on + strand, within Pf1N1B4_1544 at 1780.680 kb on + strand, within Pf1N1B4_1544 at 1780.717 kb on - strand at 1780.717 kb on - strand, within Pf1N1B4_1544 at 1780.749 kb on + strand, within Pf1N1B4_1544 at 1780.749 kb on + strand, within Pf1N1B4_1544 at 1780.803 kb on + strand, within Pf1N1B4_1544 at 1781.310 kb on + strand, within Pf1N1B4_1544 at 1781.310 kb on + strand, within Pf1N1B4_1544 at 1781.310 kb on + strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.311 kb on - strand, within Pf1N1B4_1544 at 1781.392 kb on - strand, within Pf1N1B4_1544 at 1781.393 kb on + strand, within Pf1N1B4_1544 at 1781.423 kb on + strand, within Pf1N1B4_1544 at 1781.424 kb on - strand, within Pf1N1B4_1544 at 1781.424 kb on - strand, within Pf1N1B4_1544 at 1781.424 kb on - strand, within Pf1N1B4_1544 at 1781.518 kb on - strand, within Pf1N1B4_1544 at 1781.583 kb on + strand, within Pf1N1B4_1544 at 1781.584 kb on - strand, within Pf1N1B4_1544 at 1781.584 kb on - strand, within Pf1N1B4_1544 at 1781.706 kb on + strand, within Pf1N1B4_1544 at 1781.721 kb on + strand, within Pf1N1B4_1544 at 1781.762 kb on - strand at 1781.762 kb on - strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1782.066 kb on + strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.171 kb on + strand at 1782.171 kb on + strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.291 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.414 kb on - strand at 1782.419 kb on + strand at 1782.419 kb on + strand at 1782.420 kb on - strand at 1782.420 kb on - strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.593 kb on + strand at 1782.594 kb on - strand at 1782.696 kb on + strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.837 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1783.227 kb on + strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,778,979 - Pf1N1B4_1543 0.28 +1.7 1,779,038 + Pf1N1B4_1543 0.34 +0.1 1,779,095 + Pf1N1B4_1543 0.39 -0.8 1,779,095 + Pf1N1B4_1543 0.39 -1.5 1,779,095 + Pf1N1B4_1543 0.39 +0.5 1,779,095 + Pf1N1B4_1543 0.39 +0.9 1,779,095 + Pf1N1B4_1543 0.39 +0.0 1,779,095 + Pf1N1B4_1543 0.39 +0.6 1,779,096 - Pf1N1B4_1543 0.39 -0.9 1,779,096 - Pf1N1B4_1543 0.39 +3.1 1,779,227 + Pf1N1B4_1543 0.51 +1.7 1,779,228 - Pf1N1B4_1543 0.51 -0.2 1,779,404 + Pf1N1B4_1543 0.68 +0.3 1,779,404 + Pf1N1B4_1543 0.68 +0.5 1,779,404 + Pf1N1B4_1543 0.68 +0.1 1,779,404 + Pf1N1B4_1543 0.68 +0.6 1,779,404 + Pf1N1B4_1543 0.68 +1.6 1,779,404 + Pf1N1B4_1543 0.68 +0.1 1,779,404 + Pf1N1B4_1543 0.68 +0.4 1,779,405 - Pf1N1B4_1543 0.68 +0.7 1,779,405 - Pf1N1B4_1543 0.68 -0.5 1,779,585 + Pf1N1B4_1543 0.85 +0.8 1,779,629 + Pf1N1B4_1543 0.89 -0.5 1,779,630 - Pf1N1B4_1543 0.89 +1.1 1,779,664 + -0.0 1,779,664 + -1.0 1,779,665 - -0.9 1,779,756 + -0.7 1,779,854 + +1.1 1,779,854 + +1.1 1,779,854 + +0.1 1,779,854 + +0.1 1,779,854 + +1.5 1,779,856 + -0.0 1,779,856 + -0.5 1,779,856 + -0.1 1,779,856 + -0.5 1,779,906 + -0.5 1,779,927 + -1.2 1,780,085 + +2.1 1,780,210 + -0.5 1,780,301 + Pf1N1B4_1544 0.14 +0.1 1,780,449 + +1.1 1,780,464 + Pf1N1B4_1544 0.21 -0.8 1,780,509 + Pf1N1B4_1544 0.22 -1.1 1,780,509 + Pf1N1B4_1544 0.22 -1.0 1,780,651 - Pf1N1B4_1544 0.28 -0.7 1,780,651 - Pf1N1B4_1544 0.28 +1.1 1,780,651 - Pf1N1B4_1544 0.28 -2.0 1,780,652 + Pf1N1B4_1544 0.28 -1.0 1,780,680 + Pf1N1B4_1544 0.29 +0.7 1,780,717 - +0.1 1,780,717 - Pf1N1B4_1544 0.31 +0.1 1,780,749 + Pf1N1B4_1544 0.32 +0.4 1,780,749 + Pf1N1B4_1544 0.32 -1.2 1,780,803 + Pf1N1B4_1544 0.34 -1.2 1,781,310 + Pf1N1B4_1544 0.55 +0.5 1,781,310 + Pf1N1B4_1544 0.55 -0.9 1,781,310 + Pf1N1B4_1544 0.55 -1.2 1,781,311 - Pf1N1B4_1544 0.55 +0.5 1,781,311 - Pf1N1B4_1544 0.55 -1.2 1,781,311 - Pf1N1B4_1544 0.55 +0.8 1,781,311 - Pf1N1B4_1544 0.55 +0.4 1,781,311 - Pf1N1B4_1544 0.55 -0.5 1,781,311 - Pf1N1B4_1544 0.55 -0.5 1,781,392 - Pf1N1B4_1544 0.58 -0.1 1,781,393 + Pf1N1B4_1544 0.59 -1.2 1,781,423 + Pf1N1B4_1544 0.60 -0.5 1,781,424 - Pf1N1B4_1544 0.60 +0.7 1,781,424 - Pf1N1B4_1544 0.60 +0.1 1,781,424 - Pf1N1B4_1544 0.60 -0.2 1,781,518 - Pf1N1B4_1544 0.64 -1.2 1,781,583 + Pf1N1B4_1544 0.66 -1.6 1,781,584 - Pf1N1B4_1544 0.66 -0.5 1,781,584 - Pf1N1B4_1544 0.66 +1.5 1,781,706 + Pf1N1B4_1544 0.71 +0.5 1,781,721 + Pf1N1B4_1544 0.72 +0.1 1,781,762 - -0.5 1,781,762 - Pf1N1B4_1544 0.74 +0.3 1,781,934 + Pf1N1B4_1544 0.81 -0.0 1,781,934 + Pf1N1B4_1544 0.81 -1.2 1,781,934 + Pf1N1B4_1544 0.81 -0.8 1,781,934 + Pf1N1B4_1544 0.81 -0.2 1,781,934 + Pf1N1B4_1544 0.81 -0.1 1,781,934 + Pf1N1B4_1544 0.81 +0.5 1,781,934 + Pf1N1B4_1544 0.81 -1.6 1,781,934 + Pf1N1B4_1544 0.81 +0.5 1,781,934 + Pf1N1B4_1544 0.81 +1.1 1,781,935 - Pf1N1B4_1544 0.81 -0.5 1,781,935 - Pf1N1B4_1544 0.81 -0.9 1,781,935 - Pf1N1B4_1544 0.81 +0.1 1,781,935 - -0.5 1,781,935 - Pf1N1B4_1544 0.81 +1.1 1,781,935 - Pf1N1B4_1544 0.81 +0.4 1,781,935 - Pf1N1B4_1544 0.81 -0.0 1,781,935 - Pf1N1B4_1544 0.81 -0.3 1,781,935 - Pf1N1B4_1544 0.81 -0.9 1,781,935 - Pf1N1B4_1544 0.81 -0.6 1,781,935 - Pf1N1B4_1544 0.81 -0.7 1,782,066 + Pf1N1B4_1544 0.86 -1.9 1,782,079 - Pf1N1B4_1544 0.87 +0.1 1,782,079 - Pf1N1B4_1544 0.87 +2.1 1,782,171 + -0.9 1,782,171 + -0.5 1,782,172 - -0.5 1,782,172 - +1.4 1,782,172 - +1.1 1,782,172 - +0.4 1,782,291 + +1.1 1,782,413 + -0.7 1,782,413 + -0.1 1,782,413 + -0.7 1,782,413 + +0.6 1,782,413 + -0.3 1,782,414 - -0.6 1,782,419 + -0.5 1,782,419 + -0.3 1,782,420 - -0.5 1,782,420 - +0.1 1,782,423 + -1.5 1,782,423 + +0.3 1,782,423 + -1.6 1,782,424 - +2.7 1,782,424 - +0.0 1,782,424 - -1.5 1,782,424 - -1.5 1,782,593 + +0.5 1,782,594 - -1.7 1,782,696 + -1.5 1,782,697 - +0.7 1,782,697 - -1.7 1,782,697 - +0.1 1,782,804 + Pf1N1B4_1545 0.11 +0.7 1,782,804 + Pf1N1B4_1545 0.11 +0.1 1,782,804 + Pf1N1B4_1545 0.11 -0.5 1,782,804 + Pf1N1B4_1545 0.11 +0.1 1,782,804 + Pf1N1B4_1545 0.11 +2.5 1,782,804 + Pf1N1B4_1545 0.11 +1.5 1,782,804 + Pf1N1B4_1545 0.11 +0.4 1,782,805 - Pf1N1B4_1545 0.12 +1.1 1,782,805 - Pf1N1B4_1545 0.12 +0.1 1,782,805 - Pf1N1B4_1545 0.12 +1.8 1,782,805 - Pf1N1B4_1545 0.12 -0.7 1,782,805 - Pf1N1B4_1545 0.12 -0.3 1,782,805 - Pf1N1B4_1545 0.12 -0.0 1,782,805 - Pf1N1B4_1545 0.12 +0.1 1,782,805 - Pf1N1B4_1545 0.12 +0.1 1,782,837 + Pf1N1B4_1545 0.14 +0.1 1,782,974 + Pf1N1B4_1545 0.25 -0.2 1,782,974 + Pf1N1B4_1545 0.25 -0.5 1,782,974 + Pf1N1B4_1545 0.25 +0.8 1,782,975 - Pf1N1B4_1545 0.25 +1.9 1,782,975 - Pf1N1B4_1545 0.25 +0.8 1,782,975 - Pf1N1B4_1545 0.25 +0.5 1,783,227 + Pf1N1B4_1545 0.45 +0.1 1,783,228 - Pf1N1B4_1545 0.45 -0.0 1,783,228 - Pf1N1B4_1545 0.45 +0.3 1,783,228 - Pf1N1B4_1545 0.45 +0.7 1,783,281 + Pf1N1B4_1545 0.49 +1.1 1,783,281 + Pf1N1B4_1545 0.49 -0.4 1,783,281 + Pf1N1B4_1545 0.49 -0.7 1,783,281 + Pf1N1B4_1545 0.49 -1.5 1,783,281 + Pf1N1B4_1545 0.49 -0.5 1,783,281 + +1.1 1,783,281 + Pf1N1B4_1545 0.49 +0.3 1,783,281 + Pf1N1B4_1545 0.49 +0.4 1,783,282 - Pf1N1B4_1545 0.49 -0.2 1,783,282 - Pf1N1B4_1545 0.49 -0.2 1,783,282 - Pf1N1B4_1545 0.49 -0.3 1,783,282 - Pf1N1B4_1545 0.49 -1.9 1,783,282 - Pf1N1B4_1545 0.49 -0.5 1,783,282 - Pf1N1B4_1545 0.49 -0.9 1,783,282 - Pf1N1B4_1545 0.49 +0.2 1,783,282 - Pf1N1B4_1545 0.49 +0.3 1,783,282 - Pf1N1B4_1545 0.49 +0.9 1,783,282 - Pf1N1B4_1545 0.49 +1.1 1,783,282 - Pf1N1B4_1545 0.49 -0.9 1,783,282 - Pf1N1B4_1545 0.49 +0.6 1,783,282 - Pf1N1B4_1545 0.49 +0.1 1,783,282 - Pf1N1B4_1545 0.49 -0.1 1,783,282 - Pf1N1B4_1545 0.49 +0.4 1,783,282 - Pf1N1B4_1545 0.49 +0.7
Or see this region's nucleotide sequence