Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1387 and Pf1N1B4_1388 are separated by 192 nucleotides Pf1N1B4_1388 and Pf1N1B4_1389 are separated by 344 nucleotides Pf1N1B4_1389 and Pf1N1B4_1390 are separated by 68 nucleotides Pf1N1B4_1390 and Pf1N1B4_1391 are separated by 220 nucleotides
Pf1N1B4_1387 - NAD synthetase (EC 6.3.1.5), at 1,587,794 to 1,588,621
_1387
Pf1N1B4_1388 - Azurin, at 1,588,814 to 1,589,260
_1388
Pf1N1B4_1389 - Lysine decarboxylase family, at 1,589,605 to 1,590,192
_1389
Pf1N1B4_1390 - FIG00956548: hypothetical protein, at 1,590,261 to 1,590,449
_1390
Pf1N1B4_1391 - Protein containing transglutaminase-like domain, putative cysteine protease, at 1,590,670 to 1,591,560
_1391
Position (kb)
1589
1590
1591 Strain fitness (log2 ratio)
-2
-1
0
1 at 1588.755 kb on - strand at 1588.811 kb on + strand at 1588.879 kb on - strand, within Pf1N1B4_1388 at 1588.986 kb on + strand, within Pf1N1B4_1388 at 1588.986 kb on + strand, within Pf1N1B4_1388 at 1588.986 kb on + strand, within Pf1N1B4_1388 at 1589.062 kb on + strand, within Pf1N1B4_1388 at 1589.063 kb on - strand, within Pf1N1B4_1388 at 1589.370 kb on - strand at 1589.370 kb on - strand at 1589.391 kb on + strand at 1589.391 kb on + strand at 1589.392 kb on - strand at 1589.392 kb on - strand at 1589.418 kb on - strand at 1589.495 kb on + strand at 1589.543 kb on + strand at 1589.572 kb on + strand at 1589.572 kb on + strand at 1589.573 kb on - strand at 1589.573 kb on - strand at 1589.599 kb on + strand at 1589.599 kb on + strand at 1589.600 kb on - strand at 1589.600 kb on - strand at 1589.600 kb on - strand at 1589.613 kb on - strand at 1589.627 kb on + strand at 1589.652 kb on + strand at 1589.662 kb on + strand at 1589.913 kb on + strand, within Pf1N1B4_1389 at 1589.913 kb on + strand, within Pf1N1B4_1389 at 1589.914 kb on - strand, within Pf1N1B4_1389 at 1590.014 kb on + strand, within Pf1N1B4_1389 at 1590.014 kb on + strand, within Pf1N1B4_1389 at 1590.015 kb on - strand, within Pf1N1B4_1389 at 1590.055 kb on - strand, within Pf1N1B4_1389 at 1590.055 kb on - strand, within Pf1N1B4_1389 at 1590.267 kb on - strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.273 kb on - strand at 1590.273 kb on - strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.351 kb on - strand, within Pf1N1B4_1390 at 1590.685 kb on + strand at 1590.686 kb on - strand at 1590.686 kb on - strand at 1590.686 kb on - strand at 1590.935 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,588,755 - +0.1 1,588,811 + +0.1 1,588,879 - Pf1N1B4_1388 0.15 +0.1 1,588,986 + Pf1N1B4_1388 0.38 +0.7 1,588,986 + Pf1N1B4_1388 0.38 -1.5 1,588,986 + Pf1N1B4_1388 0.38 -0.4 1,589,062 + Pf1N1B4_1388 0.55 +0.0 1,589,063 - Pf1N1B4_1388 0.56 -0.7 1,589,370 - -0.6 1,589,370 - -0.5 1,589,391 + +0.5 1,589,391 + -0.9 1,589,392 - +0.4 1,589,392 - +1.4 1,589,418 - -0.3 1,589,495 + -1.5 1,589,543 + -0.9 1,589,572 + -0.9 1,589,572 + +0.1 1,589,573 - +1.7 1,589,573 - -1.0 1,589,599 + -0.1 1,589,599 + +1.1 1,589,600 - -0.1 1,589,600 - -1.5 1,589,600 - +0.8 1,589,613 - +0.2 1,589,627 + +1.1 1,589,652 + -0.2 1,589,662 + -0.4 1,589,913 + Pf1N1B4_1389 0.52 -0.5 1,589,913 + Pf1N1B4_1389 0.52 +0.7 1,589,914 - Pf1N1B4_1389 0.53 -0.6 1,590,014 + Pf1N1B4_1389 0.70 +0.5 1,590,014 + Pf1N1B4_1389 0.70 -0.6 1,590,015 - Pf1N1B4_1389 0.70 -1.2 1,590,055 - Pf1N1B4_1389 0.77 -0.1 1,590,055 - Pf1N1B4_1389 0.77 -0.4 1,590,267 - -1.4 1,590,272 + +1.3 1,590,272 + -0.2 1,590,272 + -0.2 1,590,272 + +0.6 1,590,272 + -1.7 1,590,273 - -0.5 1,590,273 - +1.0 1,590,276 + -0.1 1,590,276 + -1.1 1,590,276 + +0.3 1,590,276 + +0.3 1,590,276 + +0.6 1,590,277 - +0.4 1,590,277 - -0.6 1,590,277 - +1.6 1,590,277 - +0.7 1,590,350 + Pf1N1B4_1390 0.47 +0.0 1,590,350 + Pf1N1B4_1390 0.47 -0.1 1,590,350 + Pf1N1B4_1390 0.47 +0.5 1,590,350 + Pf1N1B4_1390 0.47 +0.2 1,590,350 + Pf1N1B4_1390 0.47 -0.0 1,590,350 + Pf1N1B4_1390 0.47 -0.8 1,590,350 + Pf1N1B4_1390 0.47 +0.4 1,590,351 - Pf1N1B4_1390 0.48 -0.4 1,590,685 + +0.5 1,590,686 - -0.2 1,590,686 - +0.1 1,590,686 - -0.6 1,590,935 + Pf1N1B4_1391 0.30 -0.7 1,591,175 + Pf1N1B4_1391 0.57 +0.3 1,591,175 + Pf1N1B4_1391 0.57 +0.2 1,591,175 + Pf1N1B4_1391 0.57 +0.1 1,591,175 + Pf1N1B4_1391 0.57 +0.7 1,591,175 + Pf1N1B4_1391 0.57 -1.4 1,591,175 + Pf1N1B4_1391 0.57 -0.2 1,591,175 + Pf1N1B4_1391 0.57 +1.6 1,591,175 + Pf1N1B4_1391 0.57 +0.7 1,591,176 - Pf1N1B4_1391 0.57 -0.4 1,591,176 - Pf1N1B4_1391 0.57 +0.6 1,591,176 - Pf1N1B4_1391 0.57 -1.2 1,591,176 - Pf1N1B4_1391 0.57 +1.1 1,591,176 - Pf1N1B4_1391 0.57 -2.1
Or see this region's nucleotide sequence