Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1363 and Pf1N1B4_1364 are separated by 115 nucleotides Pf1N1B4_1364 and Pf1N1B4_1365 are separated by 106 nucleotides Pf1N1B4_1365 and Pf1N1B4_1366 are separated by 0 nucleotides
Pf1N1B4_1363 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,561,925 to 1,562,812
_1363
Pf1N1B4_1364 - Lipase precursor (EC 3.1.1.3), at 1,562,928 to 1,563,818
_1364
Pf1N1B4_1365 - Quaternary ammonium compound-resistance protein SugE, at 1,563,925 to 1,564,230
_1365
Pf1N1B4_1366 - FMN-dependent NADH-azoreductase, at 1,564,231 to 1,564,869
_1366
Position (kb)
1562
1563
1564 Strain fitness (log2 ratio)
-2
-1
0
1 at 1561.931 kb on + strand at 1561.931 kb on + strand at 1561.931 kb on + strand at 1562.071 kb on + strand, within Pf1N1B4_1363 at 1562.168 kb on + strand, within Pf1N1B4_1363 at 1562.168 kb on + strand, within Pf1N1B4_1363 at 1562.319 kb on + strand, within Pf1N1B4_1363 at 1562.319 kb on + strand, within Pf1N1B4_1363 at 1562.319 kb on + strand, within Pf1N1B4_1363 at 1562.417 kb on + strand, within Pf1N1B4_1363 at 1562.417 kb on + strand, within Pf1N1B4_1363 at 1562.418 kb on - strand, within Pf1N1B4_1363 at 1562.418 kb on - strand, within Pf1N1B4_1363 at 1562.471 kb on + strand, within Pf1N1B4_1363 at 1562.471 kb on + strand, within Pf1N1B4_1363 at 1562.471 kb on + strand, within Pf1N1B4_1363 at 1562.471 kb on + strand, within Pf1N1B4_1363 at 1562.471 kb on + strand, within Pf1N1B4_1363 at 1562.471 kb on + strand, within Pf1N1B4_1363 at 1562.471 kb on + strand, within Pf1N1B4_1363 at 1562.472 kb on - strand, within Pf1N1B4_1363 at 1562.472 kb on - strand, within Pf1N1B4_1363 at 1562.567 kb on + strand, within Pf1N1B4_1363 at 1562.568 kb on - strand, within Pf1N1B4_1363 at 1562.720 kb on + strand, within Pf1N1B4_1363 at 1562.720 kb on + strand, within Pf1N1B4_1363 at 1562.721 kb on - strand, within Pf1N1B4_1363 at 1562.721 kb on - strand, within Pf1N1B4_1363 at 1562.721 kb on - strand, within Pf1N1B4_1363 at 1562.794 kb on + strand at 1562.797 kb on + strand at 1562.797 kb on + strand at 1562.797 kb on + strand at 1562.814 kb on - strand at 1562.929 kb on + strand at 1563.044 kb on - strand, within Pf1N1B4_1364 at 1563.198 kb on + strand, within Pf1N1B4_1364 at 1563.198 kb on + strand, within Pf1N1B4_1364 at 1563.199 kb on - strand, within Pf1N1B4_1364 at 1563.199 kb on - strand, within Pf1N1B4_1364 at 1563.199 kb on - strand, within Pf1N1B4_1364 at 1563.199 kb on - strand, within Pf1N1B4_1364 at 1563.217 kb on + strand, within Pf1N1B4_1364 at 1563.217 kb on + strand, within Pf1N1B4_1364 at 1563.217 kb on + strand, within Pf1N1B4_1364 at 1563.218 kb on - strand, within Pf1N1B4_1364 at 1563.220 kb on + strand, within Pf1N1B4_1364 at 1563.412 kb on - strand, within Pf1N1B4_1364 at 1563.412 kb on - strand, within Pf1N1B4_1364 at 1563.459 kb on - strand, within Pf1N1B4_1364 at 1563.459 kb on - strand, within Pf1N1B4_1364 at 1563.459 kb on - strand, within Pf1N1B4_1364 at 1563.459 kb on - strand, within Pf1N1B4_1364 at 1563.459 kb on - strand, within Pf1N1B4_1364 at 1563.466 kb on + strand, within Pf1N1B4_1364 at 1563.466 kb on + strand, within Pf1N1B4_1364 at 1563.466 kb on + strand, within Pf1N1B4_1364 at 1563.466 kb on + strand, within Pf1N1B4_1364 at 1563.467 kb on - strand, within Pf1N1B4_1364 at 1563.730 kb on + strand at 1563.736 kb on + strand at 1563.914 kb on + strand at 1563.914 kb on + strand at 1563.915 kb on - strand at 1563.952 kb on + strand at 1564.101 kb on - strand, within Pf1N1B4_1365 at 1564.101 kb on - strand, within Pf1N1B4_1365 at 1564.166 kb on + strand, within Pf1N1B4_1365 at 1564.167 kb on - strand, within Pf1N1B4_1365 at 1564.167 kb on - strand, within Pf1N1B4_1365 at 1564.167 kb on - strand, within Pf1N1B4_1365 at 1564.232 kb on + strand at 1564.232 kb on + strand at 1564.232 kb on + strand at 1564.233 kb on - strand at 1564.233 kb on - strand at 1564.233 kb on - strand at 1564.233 kb on - strand at 1564.479 kb on - strand, within Pf1N1B4_1366 at 1564.479 kb on - strand, within Pf1N1B4_1366 at 1564.577 kb on + strand, within Pf1N1B4_1366 at 1564.577 kb on + strand at 1564.578 kb on - strand, within Pf1N1B4_1366 at 1564.578 kb on - strand, within Pf1N1B4_1366 at 1564.578 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,561,931 + +0.7 1,561,931 + -0.9 1,561,931 + -0.0 1,562,071 + Pf1N1B4_1363 0.16 +0.2 1,562,168 + Pf1N1B4_1363 0.27 -1.7 1,562,168 + Pf1N1B4_1363 0.27 -1.7 1,562,319 + Pf1N1B4_1363 0.44 +1.4 1,562,319 + Pf1N1B4_1363 0.44 +0.1 1,562,319 + Pf1N1B4_1363 0.44 +0.2 1,562,417 + Pf1N1B4_1363 0.55 +1.7 1,562,417 + Pf1N1B4_1363 0.55 -2.7 1,562,418 - Pf1N1B4_1363 0.56 +1.1 1,562,418 - Pf1N1B4_1363 0.56 +1.1 1,562,471 + Pf1N1B4_1363 0.61 -0.0 1,562,471 + Pf1N1B4_1363 0.61 +0.1 1,562,471 + Pf1N1B4_1363 0.61 -2.2 1,562,471 + Pf1N1B4_1363 0.61 -0.7 1,562,471 + Pf1N1B4_1363 0.61 -0.1 1,562,471 + Pf1N1B4_1363 0.61 -2.1 1,562,471 + Pf1N1B4_1363 0.61 -1.0 1,562,472 - Pf1N1B4_1363 0.62 +0.5 1,562,472 - Pf1N1B4_1363 0.62 +0.5 1,562,567 + Pf1N1B4_1363 0.72 +1.1 1,562,568 - Pf1N1B4_1363 0.72 -0.7 1,562,720 + Pf1N1B4_1363 0.90 +1.1 1,562,720 + Pf1N1B4_1363 0.90 -0.7 1,562,721 - Pf1N1B4_1363 0.90 -1.8 1,562,721 - Pf1N1B4_1363 0.90 +0.1 1,562,721 - Pf1N1B4_1363 0.90 -0.2 1,562,794 + +0.3 1,562,797 + +0.7 1,562,797 + +0.6 1,562,797 + +0.7 1,562,814 - +1.7 1,562,929 + +0.8 1,563,044 - Pf1N1B4_1364 0.13 -0.9 1,563,198 + Pf1N1B4_1364 0.30 +1.1 1,563,198 + Pf1N1B4_1364 0.30 -2.1 1,563,199 - Pf1N1B4_1364 0.30 +0.4 1,563,199 - Pf1N1B4_1364 0.30 +1.1 1,563,199 - Pf1N1B4_1364 0.30 -0.9 1,563,199 - Pf1N1B4_1364 0.30 +0.3 1,563,217 + Pf1N1B4_1364 0.32 -1.1 1,563,217 + Pf1N1B4_1364 0.32 -2.4 1,563,217 + Pf1N1B4_1364 0.32 +0.8 1,563,218 - Pf1N1B4_1364 0.33 -1.5 1,563,220 + Pf1N1B4_1364 0.33 -0.7 1,563,412 - Pf1N1B4_1364 0.54 +0.4 1,563,412 - Pf1N1B4_1364 0.54 +0.6 1,563,459 - Pf1N1B4_1364 0.60 -0.7 1,563,459 - Pf1N1B4_1364 0.60 -0.9 1,563,459 - Pf1N1B4_1364 0.60 +0.4 1,563,459 - Pf1N1B4_1364 0.60 +0.7 1,563,459 - Pf1N1B4_1364 0.60 -0.1 1,563,466 + Pf1N1B4_1364 0.60 -0.1 1,563,466 + Pf1N1B4_1364 0.60 -0.4 1,563,466 + Pf1N1B4_1364 0.60 +1.4 1,563,466 + Pf1N1B4_1364 0.60 +0.8 1,563,467 - Pf1N1B4_1364 0.60 +0.1 1,563,730 + -1.0 1,563,736 + +0.4 1,563,914 + -0.6 1,563,914 + -0.9 1,563,915 - -1.2 1,563,952 + +0.5 1,564,101 - Pf1N1B4_1365 0.58 +1.1 1,564,101 - Pf1N1B4_1365 0.58 +0.4 1,564,166 + Pf1N1B4_1365 0.79 +1.2 1,564,167 - Pf1N1B4_1365 0.79 +0.1 1,564,167 - Pf1N1B4_1365 0.79 -0.7 1,564,167 - Pf1N1B4_1365 0.79 +0.7 1,564,232 + -2.1 1,564,232 + -0.4 1,564,232 + -0.6 1,564,233 - +1.7 1,564,233 - -1.4 1,564,233 - -0.5 1,564,233 - +1.1 1,564,479 - Pf1N1B4_1366 0.39 -1.9 1,564,479 - Pf1N1B4_1366 0.39 +0.1 1,564,577 + Pf1N1B4_1366 0.54 +0.3 1,564,577 + +0.1 1,564,578 - Pf1N1B4_1366 0.54 -1.1 1,564,578 - Pf1N1B4_1366 0.54 -1.2 1,564,578 - +1.1
Or see this region's nucleotide sequence