Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1281 and Pf1N1B4_1282 are separated by 922 nucleotides Pf1N1B4_1282 and Pf1N1B4_1283 are separated by 113 nucleotides Pf1N1B4_1283 and Pf1N1B4_1284 are separated by 111 nucleotides
Pf1N1B4_1281 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 1,461,858 to 1,462,079
_1281
Pf1N1B4_1282 - DNA-binding response regulator, LuxR family, at 1,463,002 to 1,463,790
_1282
Pf1N1B4_1283 - Flp pilus assembly protein CpaA, at 1,463,904 to 1,464,374
_1283
Pf1N1B4_1284 - Flp pilus assembly protein TadG, at 1,464,486 to 1,464,890
_1284
Position (kb)
1463
1464 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.075 kb on + strand at 1462.239 kb on + strand at 1462.239 kb on + strand at 1462.239 kb on + strand at 1462.239 kb on + strand at 1462.239 kb on + strand at 1462.239 kb on + strand at 1462.239 kb on + strand at 1462.239 kb on + strand at 1462.240 kb on - strand at 1462.240 kb on - strand at 1462.303 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.334 kb on + strand at 1462.335 kb on - strand at 1462.335 kb on - strand at 1462.335 kb on - strand at 1462.335 kb on - strand at 1462.344 kb on + strand at 1462.355 kb on - strand at 1462.423 kb on + strand at 1462.424 kb on - strand at 1462.549 kb on + strand at 1462.549 kb on + strand at 1462.549 kb on + strand at 1462.549 kb on + strand at 1462.549 kb on + strand at 1462.549 kb on + strand at 1462.550 kb on - strand at 1462.550 kb on - strand at 1462.550 kb on - strand at 1462.552 kb on - strand at 1462.552 kb on - strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.557 kb on + strand at 1462.558 kb on - strand at 1462.558 kb on - strand at 1462.558 kb on - strand at 1462.558 kb on - strand at 1462.558 kb on - strand at 1462.558 kb on - strand at 1462.558 kb on - strand at 1462.610 kb on + strand at 1462.671 kb on - strand at 1462.827 kb on + strand at 1462.828 kb on - strand at 1462.828 kb on - strand at 1462.843 kb on + strand at 1462.843 kb on + strand at 1462.844 kb on - strand at 1462.844 kb on - strand at 1462.911 kb on + strand at 1462.911 kb on + strand at 1462.911 kb on + strand at 1462.924 kb on + strand at 1462.925 kb on - strand at 1462.925 kb on - strand at 1462.925 kb on - strand at 1462.925 kb on - strand at 1462.996 kb on - strand at 1463.013 kb on + strand at 1463.014 kb on - strand at 1463.035 kb on - strand at 1463.035 kb on - strand at 1463.035 kb on - strand at 1463.071 kb on - strand at 1463.076 kb on + strand at 1463.077 kb on - strand at 1463.077 kb on - strand at 1463.105 kb on + strand, within Pf1N1B4_1282 at 1463.105 kb on + strand, within Pf1N1B4_1282 at 1463.105 kb on + strand, within Pf1N1B4_1282 at 1463.106 kb on - strand, within Pf1N1B4_1282 at 1463.106 kb on - strand, within Pf1N1B4_1282 at 1463.106 kb on - strand, within Pf1N1B4_1282 at 1463.153 kb on + strand, within Pf1N1B4_1282 at 1463.154 kb on - strand, within Pf1N1B4_1282 at 1463.321 kb on + strand, within Pf1N1B4_1282 at 1463.322 kb on - strand, within Pf1N1B4_1282 at 1463.463 kb on - strand, within Pf1N1B4_1282 at 1463.465 kb on + strand, within Pf1N1B4_1282 at 1463.466 kb on - strand, within Pf1N1B4_1282 at 1463.466 kb on - strand, within Pf1N1B4_1282 at 1463.466 kb on - strand, within Pf1N1B4_1282 at 1463.767 kb on + strand at 1463.768 kb on - strand at 1463.768 kb on - strand at 1463.778 kb on + strand at 1463.779 kb on - strand at 1463.779 kb on - strand at 1463.779 kb on - strand at 1463.837 kb on + strand at 1463.837 kb on + strand at 1463.837 kb on + strand at 1463.837 kb on + strand at 1463.857 kb on + strand at 1463.857 kb on + strand at 1463.858 kb on - strand at 1463.858 kb on - strand at 1463.874 kb on + strand at 1463.874 kb on + strand at 1463.874 kb on + strand at 1463.874 kb on + strand at 1463.874 kb on + strand at 1463.874 kb on + strand at 1463.874 kb on + strand at 1463.874 kb on + strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.875 kb on - strand at 1463.878 kb on + strand at 1463.878 kb on + strand at 1463.878 kb on + strand at 1463.878 kb on + strand at 1463.878 kb on + strand at 1463.878 kb on + strand at 1463.879 kb on - strand at 1463.879 kb on - strand at 1463.879 kb on - strand at 1463.879 kb on - strand at 1463.879 kb on - strand at 1463.882 kb on + strand at 1463.882 kb on + strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.883 kb on - strand at 1463.924 kb on + strand at 1463.924 kb on + strand at 1463.925 kb on - strand at 1463.925 kb on - strand at 1463.925 kb on - strand at 1463.944 kb on - strand at 1463.990 kb on - strand, within Pf1N1B4_1283 at 1464.020 kb on + strand, within Pf1N1B4_1283 at 1464.021 kb on - strand, within Pf1N1B4_1283 at 1464.022 kb on + strand, within Pf1N1B4_1283 at 1464.022 kb on + strand, within Pf1N1B4_1283 at 1464.022 kb on + strand, within Pf1N1B4_1283 at 1464.022 kb on + strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.023 kb on - strand at 1464.023 kb on - strand, within Pf1N1B4_1283 at 1464.262 kb on + strand, within Pf1N1B4_1283 at 1464.262 kb on + strand, within Pf1N1B4_1283 at 1464.262 kb on + strand, within Pf1N1B4_1283 at 1464.262 kb on + strand, within Pf1N1B4_1283 at 1464.262 kb on + strand, within Pf1N1B4_1283 at 1464.263 kb on - strand, within Pf1N1B4_1283 at 1464.263 kb on - strand, within Pf1N1B4_1283 at 1464.263 kb on - strand, within Pf1N1B4_1283 at 1464.263 kb on - strand, within Pf1N1B4_1283 at 1464.263 kb on - strand, within Pf1N1B4_1283 at 1464.263 kb on - strand, within Pf1N1B4_1283 at 1464.276 kb on + strand, within Pf1N1B4_1283 at 1464.276 kb on + strand, within Pf1N1B4_1283 at 1464.277 kb on - strand, within Pf1N1B4_1283 at 1464.277 kb on - strand, within Pf1N1B4_1283 at 1464.353 kb on + strand at 1464.353 kb on + strand at 1464.354 kb on - strand at 1464.354 kb on - strand at 1464.354 kb on - strand at 1464.354 kb on - strand at 1464.354 kb on - strand at 1464.354 kb on - strand at 1464.354 kb on - strand at 1464.358 kb on - strand at 1464.382 kb on + strand at 1464.383 kb on - strand at 1464.383 kb on - strand at 1464.383 kb on - strand at 1464.383 kb on - strand at 1464.409 kb on + strand at 1464.409 kb on + strand at 1464.409 kb on + strand at 1464.410 kb on - strand at 1464.410 kb on - strand at 1464.410 kb on - strand at 1464.410 kb on - strand at 1464.410 kb on - strand at 1464.410 kb on - strand at 1464.420 kb on + strand at 1464.420 kb on + strand at 1464.421 kb on - strand at 1464.421 kb on - strand at 1464.421 kb on - strand at 1464.421 kb on - strand at 1464.534 kb on + strand, within Pf1N1B4_1284 at 1464.535 kb on - strand, within Pf1N1B4_1284 at 1464.535 kb on - strand, within Pf1N1B4_1284 at 1464.535 kb on - strand, within Pf1N1B4_1284 at 1464.535 kb on - strand, within Pf1N1B4_1284 at 1464.643 kb on - strand, within Pf1N1B4_1284 at 1464.664 kb on + strand at 1464.664 kb on + strand, within Pf1N1B4_1284 at 1464.665 kb on - strand, within Pf1N1B4_1284 at 1464.665 kb on - strand, within Pf1N1B4_1284 at 1464.747 kb on + strand at 1464.747 kb on + strand, within Pf1N1B4_1284 at 1464.747 kb on + strand, within Pf1N1B4_1284 at 1464.747 kb on + strand, within Pf1N1B4_1284 at 1464.747 kb on + strand, within Pf1N1B4_1284 at 1464.747 kb on + strand, within Pf1N1B4_1284 at 1464.748 kb on - strand, within Pf1N1B4_1284 at 1464.748 kb on - strand, within Pf1N1B4_1284 at 1464.748 kb on - strand, within Pf1N1B4_1284 at 1464.748 kb on - strand, within Pf1N1B4_1284
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,462,075 + +0.3 1,462,075 + -0.3 1,462,075 + +0.3 1,462,075 + +0.0 1,462,075 + +0.5 1,462,075 + -0.5 1,462,075 + -1.0 1,462,075 + -0.2 1,462,075 + +0.2 1,462,075 + +1.1 1,462,239 + +0.1 1,462,239 + +0.5 1,462,239 + +0.4 1,462,239 + -1.1 1,462,239 + +0.5 1,462,239 + -0.5 1,462,239 + -0.2 1,462,239 + +1.1 1,462,240 - +0.2 1,462,240 - -1.6 1,462,303 + -0.2 1,462,334 + -0.9 1,462,334 + +1.6 1,462,334 + -0.9 1,462,334 + -0.7 1,462,334 + -0.9 1,462,334 + -1.5 1,462,334 + +1.1 1,462,334 + +0.4 1,462,334 + +0.7 1,462,335 - -0.4 1,462,335 - +0.2 1,462,335 - +0.3 1,462,335 - -1.7 1,462,344 + +0.3 1,462,355 - -0.4 1,462,423 + +0.2 1,462,424 - -1.3 1,462,549 + +0.1 1,462,549 + -0.2 1,462,549 + -1.5 1,462,549 + +0.8 1,462,549 + +0.8 1,462,549 + +0.3 1,462,550 - +0.4 1,462,550 - -1.3 1,462,550 - +1.2 1,462,552 - -0.6 1,462,552 - +0.6 1,462,557 + -0.4 1,462,557 + +0.7 1,462,557 + -0.5 1,462,557 + -0.7 1,462,557 + +0.1 1,462,557 + -0.1 1,462,557 + +1.1 1,462,557 + +0.6 1,462,557 + -1.1 1,462,557 + +1.1 1,462,557 + -0.4 1,462,557 + -1.5 1,462,557 + -0.4 1,462,557 + -0.7 1,462,557 + -0.7 1,462,557 + +0.9 1,462,557 + -0.7 1,462,557 + +1.1 1,462,558 - +1.5 1,462,558 - +0.3 1,462,558 - -1.6 1,462,558 - +0.5 1,462,558 - -0.0 1,462,558 - +0.2 1,462,558 - +0.2 1,462,610 + +1.5 1,462,671 - +0.1 1,462,827 + +0.7 1,462,828 - -1.3 1,462,828 - -1.9 1,462,843 + -0.9 1,462,843 + +1.1 1,462,844 - +0.5 1,462,844 - -0.3 1,462,911 + -0.9 1,462,911 + -1.3 1,462,911 + +0.6 1,462,924 + +1.1 1,462,925 - +3.1 1,462,925 - +0.6 1,462,925 - +0.1 1,462,925 - -1.5 1,462,996 - +1.5 1,463,013 + -0.3 1,463,014 - +0.7 1,463,035 - -0.1 1,463,035 - +2.6 1,463,035 - +0.8 1,463,071 - +0.5 1,463,076 + +0.4 1,463,077 - +0.9 1,463,077 - +0.1 1,463,105 + Pf1N1B4_1282 0.13 -0.7 1,463,105 + Pf1N1B4_1282 0.13 -1.9 1,463,105 + Pf1N1B4_1282 0.13 +1.1 1,463,106 - Pf1N1B4_1282 0.13 -1.3 1,463,106 - Pf1N1B4_1282 0.13 -0.9 1,463,106 - Pf1N1B4_1282 0.13 -2.1 1,463,153 + Pf1N1B4_1282 0.19 -0.3 1,463,154 - Pf1N1B4_1282 0.19 -1.7 1,463,321 + Pf1N1B4_1282 0.40 -0.9 1,463,322 - Pf1N1B4_1282 0.41 -0.2 1,463,463 - Pf1N1B4_1282 0.58 -1.5 1,463,465 + Pf1N1B4_1282 0.59 +0.7 1,463,466 - Pf1N1B4_1282 0.59 +0.6 1,463,466 - Pf1N1B4_1282 0.59 +0.5 1,463,466 - Pf1N1B4_1282 0.59 -0.5 1,463,767 + -0.7 1,463,768 - +0.1 1,463,768 - +0.6 1,463,778 + +1.6 1,463,779 - +1.6 1,463,779 - -0.9 1,463,779 - -0.1 1,463,837 + +0.3 1,463,837 + -0.2 1,463,837 + +1.1 1,463,837 + -0.3 1,463,857 + +0.9 1,463,857 + +0.1 1,463,858 - +0.5 1,463,858 - -0.3 1,463,874 + -0.1 1,463,874 + +1.1 1,463,874 + +0.9 1,463,874 + +1.2 1,463,874 + +2.1 1,463,874 + -0.9 1,463,874 + -0.4 1,463,874 + +0.1 1,463,875 - -1.1 1,463,875 - +1.3 1,463,875 - +0.7 1,463,875 - -1.3 1,463,875 - +0.7 1,463,875 - -0.3 1,463,875 - -0.5 1,463,875 - -0.9 1,463,875 - +0.6 1,463,875 - +1.1 1,463,875 - -0.2 1,463,878 + -0.2 1,463,878 + +0.4 1,463,878 + +0.5 1,463,878 + +1.1 1,463,878 + -0.7 1,463,878 + +2.1 1,463,879 - +1.5 1,463,879 - +0.1 1,463,879 - -0.5 1,463,879 - -1.1 1,463,879 - +0.8 1,463,882 + -0.9 1,463,882 + +0.6 1,463,883 - -0.4 1,463,883 - -0.2 1,463,883 - -0.1 1,463,883 - +1.1 1,463,883 - -0.7 1,463,883 - -0.6 1,463,883 - +0.1 1,463,883 - +0.1 1,463,883 - +0.3 1,463,883 - +0.1 1,463,924 + +2.1 1,463,924 + -0.5 1,463,925 - +1.1 1,463,925 - +1.1 1,463,925 - -0.3 1,463,944 - +1.1 1,463,990 - Pf1N1B4_1283 0.18 +0.1 1,464,020 + Pf1N1B4_1283 0.25 +0.9 1,464,021 - Pf1N1B4_1283 0.25 -0.7 1,464,022 + Pf1N1B4_1283 0.25 +0.5 1,464,022 + Pf1N1B4_1283 0.25 -0.5 1,464,022 + Pf1N1B4_1283 0.25 -0.9 1,464,022 + Pf1N1B4_1283 0.25 -2.1 1,464,023 - Pf1N1B4_1283 0.25 -1.0 1,464,023 - Pf1N1B4_1283 0.25 -0.9 1,464,023 - Pf1N1B4_1283 0.25 +0.6 1,464,023 - Pf1N1B4_1283 0.25 -1.7 1,464,023 - Pf1N1B4_1283 0.25 +0.8 1,464,023 - Pf1N1B4_1283 0.25 +0.5 1,464,023 - Pf1N1B4_1283 0.25 +0.5 1,464,023 - Pf1N1B4_1283 0.25 -0.1 1,464,023 - Pf1N1B4_1283 0.25 -1.3 1,464,023 - Pf1N1B4_1283 0.25 -0.6 1,464,023 - -0.7 1,464,023 - Pf1N1B4_1283 0.25 -0.9 1,464,262 + Pf1N1B4_1283 0.76 +0.2 1,464,262 + Pf1N1B4_1283 0.76 +0.5 1,464,262 + Pf1N1B4_1283 0.76 -0.5 1,464,262 + Pf1N1B4_1283 0.76 +1.1 1,464,262 + Pf1N1B4_1283 0.76 +0.7 1,464,263 - Pf1N1B4_1283 0.76 -0.5 1,464,263 - Pf1N1B4_1283 0.76 +0.5 1,464,263 - Pf1N1B4_1283 0.76 +1.9 1,464,263 - Pf1N1B4_1283 0.76 -0.7 1,464,263 - Pf1N1B4_1283 0.76 +0.8 1,464,263 - Pf1N1B4_1283 0.76 +0.5 1,464,276 + Pf1N1B4_1283 0.79 +0.7 1,464,276 + Pf1N1B4_1283 0.79 -0.4 1,464,277 - Pf1N1B4_1283 0.79 +0.4 1,464,277 - Pf1N1B4_1283 0.79 +0.1 1,464,353 + -0.1 1,464,353 + +0.4 1,464,354 - +0.3 1,464,354 - +0.5 1,464,354 - -0.5 1,464,354 - +1.1 1,464,354 - +0.5 1,464,354 - +0.3 1,464,354 - +0.3 1,464,358 - +1.5 1,464,382 + +1.1 1,464,383 - +0.3 1,464,383 - -0.5 1,464,383 - +0.8 1,464,383 - +0.6 1,464,409 + +1.4 1,464,409 + -0.3 1,464,409 + -0.4 1,464,410 - +1.1 1,464,410 - +0.9 1,464,410 - +0.6 1,464,410 - +0.4 1,464,410 - +0.7 1,464,410 - +0.1 1,464,420 + +1.5 1,464,420 + +0.1 1,464,421 - +0.5 1,464,421 - +1.1 1,464,421 - +1.1 1,464,421 - +0.8 1,464,534 + Pf1N1B4_1284 0.12 +1.2 1,464,535 - Pf1N1B4_1284 0.12 -0.4 1,464,535 - Pf1N1B4_1284 0.12 -1.1 1,464,535 - Pf1N1B4_1284 0.12 +1.6 1,464,535 - Pf1N1B4_1284 0.12 -0.7 1,464,643 - Pf1N1B4_1284 0.39 +0.7 1,464,664 + -0.5 1,464,664 + Pf1N1B4_1284 0.44 -0.6 1,464,665 - Pf1N1B4_1284 0.44 -0.1 1,464,665 - Pf1N1B4_1284 0.44 +0.7 1,464,747 + -0.7 1,464,747 + Pf1N1B4_1284 0.64 -0.4 1,464,747 + Pf1N1B4_1284 0.64 +1.8 1,464,747 + Pf1N1B4_1284 0.64 -0.5 1,464,747 + Pf1N1B4_1284 0.64 +0.4 1,464,747 + Pf1N1B4_1284 0.64 +0.3 1,464,748 - Pf1N1B4_1284 0.65 +0.5 1,464,748 - Pf1N1B4_1284 0.65 +0.3 1,464,748 - Pf1N1B4_1284 0.65 -0.5 1,464,748 - Pf1N1B4_1284 0.65 +0.3
Or see this region's nucleotide sequence