Experiment: 5-Hydroxymethylfurfural 2.5 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_1251 and Pf1N1B4_1252 are separated by 81 nucleotides Pf1N1B4_1252 and Pf1N1B4_1253 are separated by 222 nucleotides 
        Pf1N1B4_1251 - 2-Oxobutyrate oxidase, putative, at 1,429,802 to 1,430,767 
        _1251 
         
        
        Pf1N1B4_1252 - Adenosine deaminase (EC 3.5.4.4), at 1,430,849 to 1,431,802 
        _1252 
         
        
        Pf1N1B4_1253 - Niacin transporter NiaP, at 1,432,025 to 1,433,407 
        _1253 
         Position (kb) 1430 
1431 
1432 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 1429.913 kb on + strand, within Pf1N1B4_1251 at 1429.913 kb on + strand, within Pf1N1B4_1251 at 1429.914 kb on - strand at 1429.914 kb on - strand, within Pf1N1B4_1251 at 1429.914 kb on - strand, within Pf1N1B4_1251 at 1429.915 kb on + strand, within Pf1N1B4_1251 at 1429.916 kb on - strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.424 kb on - strand at 1430.685 kb on - strand at 1430.685 kb on - strand at 1430.685 kb on - strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.735 kb on + strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.736 kb on - strand at 1430.766 kb on - strand at 1430.766 kb on - strand at 1430.813 kb on + strand at 1430.813 kb on + strand at 1430.813 kb on + strand at 1430.813 kb on + strand at 1430.814 kb on - strand at 1430.814 kb on - strand at 1430.814 kb on - strand at 1430.814 kb on - strand at 1430.852 kb on + strand at 1430.853 kb on - strand at 1430.853 kb on - strand at 1430.993 kb on + strand, within Pf1N1B4_1252 at 1430.993 kb on + strand, within Pf1N1B4_1252 at 1430.994 kb on - strand, within Pf1N1B4_1252 at 1431.029 kb on + strand, within Pf1N1B4_1252 at 1431.029 kb on + strand, within Pf1N1B4_1252 at 1431.029 kb on + strand, within Pf1N1B4_1252 at 1431.030 kb on - strand, within Pf1N1B4_1252 at 1431.030 kb on - strand, within Pf1N1B4_1252 at 1431.030 kb on - strand at 1431.033 kb on - strand at 1431.033 kb on - strand, within Pf1N1B4_1252 at 1431.071 kb on + strand, within Pf1N1B4_1252 at 1431.089 kb on + strand, within Pf1N1B4_1252 at 1431.455 kb on + strand, within Pf1N1B4_1252 at 1431.456 kb on - strand, within Pf1N1B4_1252 at 1431.684 kb on - strand, within Pf1N1B4_1252 at 1431.695 kb on + strand, within Pf1N1B4_1252 at 1431.696 kb on - strand, within Pf1N1B4_1252 at 1431.696 kb on - strand, within Pf1N1B4_1252 at 1431.723 kb on - strand at 1431.723 kb on - strand at 1431.800 kb on + strand at 1431.801 kb on - strand at 1431.833 kb on + strand at 1431.833 kb on + strand at 1431.833 kb on + strand at 1431.834 kb on - strand at 1431.834 kb on - strand at 1431.841 kb on + strand at 1431.841 kb on + strand at 1431.841 kb on + strand at 1431.841 kb on + strand at 1431.841 kb on + strand at 1431.841 kb on + strand at 1431.841 kb on + strand at 1431.842 kb on - strand at 1431.842 kb on - strand at 1431.842 kb on - strand at 1431.842 kb on - strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.847 kb on + strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.848 kb on - strand at 1431.891 kb on + strand at 1431.891 kb on + strand at 1431.891 kb on + strand at 1431.891 kb on + strand at 1431.892 kb on - strand at 1431.892 kb on - strand at 1431.892 kb on - strand at 1431.901 kb on + strand at 1431.902 kb on - strand at 1431.913 kb on + strand at 1431.913 kb on + strand at 1431.913 kb on + strand at 1431.914 kb on - strand at 1431.921 kb on - strand at 1431.921 kb on - strand at 1431.921 kb on - strand at 1431.921 kb on - strand at 1432.169 kb on + strand, within Pf1N1B4_1253 at 1432.203 kb on - strand, within Pf1N1B4_1253 at 1432.258 kb on - strand, within Pf1N1B4_1253 at 1432.272 kb on + strand, within Pf1N1B4_1253 at 1432.273 kb on - strand at 1432.273 kb on - strand, within Pf1N1B4_1253 at 1432.273 kb on - strand, within Pf1N1B4_1253 at 1432.273 kb on - strand, within Pf1N1B4_1253 at 1432.273 kb on - strand, within Pf1N1B4_1253 at 1432.273 kb on - strand, within Pf1N1B4_1253 at 1432.273 kb on - strand, within Pf1N1B4_1253 at 1432.279 kb on - strand, within Pf1N1B4_1253 at 1432.279 kb on - strand, within Pf1N1B4_1253 at 1432.331 kb on - strand, within Pf1N1B4_1253 at 1432.331 kb on - strand, within Pf1N1B4_1253 at 1432.375 kb on - strand, within Pf1N1B4_1253 at 1432.500 kb on + strand, within Pf1N1B4_1253 at 1432.501 kb on - strand, within Pf1N1B4_1253 at 1432.501 kb on - strand, within Pf1N1B4_1253 at 1432.503 kb on + strand, within Pf1N1B4_1253 at 1432.503 kb on + strand, within Pf1N1B4_1253 at 1432.504 kb on - strand, within Pf1N1B4_1253 at 1432.504 kb on - strand, within Pf1N1B4_1253 at 1432.504 kb on - strand, within Pf1N1B4_1253 at 1432.504 kb on - strand, within Pf1N1B4_1253 at 1432.570 kb on - strand, within Pf1N1B4_1253  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,429,913 +  Pf1N1B4_1251 0.11  -1.3 1,429,913 +  Pf1N1B4_1251 0.11  +0.1 1,429,914 -  -1.5 1,429,914 -  Pf1N1B4_1251 0.12  -1.3 1,429,914 -  Pf1N1B4_1251 0.12  -2.4 1,429,915 +  Pf1N1B4_1251 0.12  -0.9 1,429,916 -  Pf1N1B4_1251 0.12  +1.7 1,430,219 +  Pf1N1B4_1251 0.43  +0.3 1,430,219 +  Pf1N1B4_1251 0.43  -0.9 1,430,219 +  Pf1N1B4_1251 0.43  -1.7 1,430,219 +  Pf1N1B4_1251 0.43  +0.4 1,430,219 +  Pf1N1B4_1251 0.43  +0.1 1,430,219 +  Pf1N1B4_1251 0.43  -0.2 1,430,220 -  Pf1N1B4_1251 0.43  +0.5 1,430,220 -  Pf1N1B4_1251 0.43  -0.5 1,430,220 -  Pf1N1B4_1251 0.43  +1.8 1,430,424 -  +1.1 1,430,685 -  -1.7 1,430,685 -  +0.5 1,430,685 -  -2.4 1,430,735 +  +2.1 1,430,735 +  +0.8 1,430,735 +  -0.3 1,430,735 +  +0.7 1,430,735 +  -0.3 1,430,736 -  +0.9 1,430,736 -  -2.6 1,430,736 -  +0.7 1,430,736 -  +0.3 1,430,736 -  -0.3 1,430,736 -  +0.6 1,430,736 -  +0.1 1,430,736 -  +1.1 1,430,736 -  +0.8 1,430,736 -  -0.6 1,430,766 -  -0.1 1,430,766 -  -0.1 1,430,813 +  +0.1 1,430,813 +  +1.5 1,430,813 +  +1.1 1,430,813 +  -0.9 1,430,814 -  -0.6 1,430,814 -  +1.9 1,430,814 -  -1.5 1,430,814 -  -1.1 1,430,852 +  -0.5 1,430,853 -  +1.1 1,430,853 -  +0.1 1,430,993 +  Pf1N1B4_1252 0.15  +1.5 1,430,993 +  Pf1N1B4_1252 0.15  +0.5 1,430,994 -  Pf1N1B4_1252 0.15  -0.3 1,431,029 +  Pf1N1B4_1252 0.19  +0.1 1,431,029 +  Pf1N1B4_1252 0.19  +0.1 1,431,029 +  Pf1N1B4_1252 0.19  +0.7 1,431,030 -  Pf1N1B4_1252 0.19  +1.1 1,431,030 -  Pf1N1B4_1252 0.19  +0.2 1,431,030 -  +0.1 1,431,033 -  +0.1 1,431,033 -  Pf1N1B4_1252 0.19  +0.7 1,431,071 +  Pf1N1B4_1252 0.23  +0.5 1,431,089 +  Pf1N1B4_1252 0.25  +0.3 1,431,455 +  Pf1N1B4_1252 0.64  +1.5 1,431,456 -  Pf1N1B4_1252 0.64  +1.1 1,431,684 -  Pf1N1B4_1252 0.88  +1.5 1,431,695 +  Pf1N1B4_1252 0.89  -1.7 1,431,696 -  Pf1N1B4_1252 0.89  +1.1 1,431,696 -  Pf1N1B4_1252 0.89  -0.9 1,431,723 -  +0.1 1,431,723 -  +1.1 1,431,800 +  +1.3 1,431,801 -  -0.9 1,431,833 +  +0.1 1,431,833 +  -0.5 1,431,833 +  -1.5 1,431,834 -  +0.1 1,431,834 -  -0.5 1,431,841 +  +0.4 1,431,841 +  +0.2 1,431,841 +  -1.9 1,431,841 +  +0.8 1,431,841 +  +0.1 1,431,841 +  -1.7 1,431,841 +  -0.1 1,431,842 -  -1.3 1,431,842 -  -0.0 1,431,842 -  -0.4 1,431,842 -  +0.8 1,431,847 +  -1.4 1,431,847 +  -0.1 1,431,847 +  -0.5 1,431,847 +  -0.5 1,431,847 +  +0.1 1,431,847 +  -0.4 1,431,847 +  +0.7 1,431,847 +  -0.7 1,431,847 +  -1.3 1,431,847 +  -0.5 1,431,848 -  +0.8 1,431,848 -  -0.3 1,431,848 -  +2.4 1,431,848 -  +0.2 1,431,848 -  +0.1 1,431,848 -  +1.1 1,431,848 -  -2.3 1,431,848 -  +0.5 1,431,848 -  +0.5 1,431,848 -  +1.1 1,431,848 -  +1.4 1,431,848 -  -0.8 1,431,891 +  -0.1 1,431,891 +  -0.0 1,431,891 +  +0.5 1,431,891 +  -1.3 1,431,892 -  +1.1 1,431,892 -  +0.9 1,431,892 -  +0.1 1,431,901 +  -1.0 1,431,902 -  +1.7 1,431,913 +  +0.1 1,431,913 +  +1.5 1,431,913 +  +1.1 1,431,914 -  +0.1 1,431,921 -  +0.7 1,431,921 -  -1.9 1,431,921 -  +0.7 1,431,921 -  -2.0 1,432,169 +  Pf1N1B4_1253 0.10  -0.3 1,432,203 -  Pf1N1B4_1253 0.13  +0.3 1,432,258 -  Pf1N1B4_1253 0.17  -0.8 1,432,272 +  Pf1N1B4_1253 0.18  +1.1 1,432,273 -  +0.1 1,432,273 -  Pf1N1B4_1253 0.18  -0.1 1,432,273 -  Pf1N1B4_1253 0.18  +0.5 1,432,273 -  Pf1N1B4_1253 0.18  -0.3 1,432,273 -  Pf1N1B4_1253 0.18  +0.5 1,432,273 -  Pf1N1B4_1253 0.18  -0.1 1,432,273 -  Pf1N1B4_1253 0.18  -0.7 1,432,279 -  Pf1N1B4_1253 0.18  +0.7 1,432,279 -  Pf1N1B4_1253 0.18  -0.4 1,432,331 -  Pf1N1B4_1253 0.22  -1.9 1,432,331 -  Pf1N1B4_1253 0.22  -0.2 1,432,375 -  Pf1N1B4_1253 0.25  +0.5 1,432,500 +  Pf1N1B4_1253 0.34  +0.3 1,432,501 -  Pf1N1B4_1253 0.34  -0.5 1,432,501 -  Pf1N1B4_1253 0.34  +0.7 1,432,503 +  Pf1N1B4_1253 0.35  -0.1 1,432,503 +  Pf1N1B4_1253 0.35  +1.1 1,432,504 -  Pf1N1B4_1253 0.35  -0.2 1,432,504 -  Pf1N1B4_1253 0.35  +0.7 1,432,504 -  Pf1N1B4_1253 0.35  -0.3 1,432,504 -  Pf1N1B4_1253 0.35  -2.3 1,432,570 -  Pf1N1B4_1253 0.39  +0.7 
Or see this region's nucleotide sequence