Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1245 and Pf1N1B4_1246 are separated by 80 nucleotides Pf1N1B4_1246 and Pf1N1B4_1247 are separated by 82 nucleotides Pf1N1B4_1247 and Pf1N1B4_1248 are separated by 25 nucleotides Pf1N1B4_1248 and Pf1N1B4_1249 are separated by 254 nucleotides
Pf1N1B4_1245 - Nucleoside ABC transporter, permease protein 2, at 1,424,043 to 1,424,969
_1245
Pf1N1B4_1246 - FIG00954047: hypothetical protein, at 1,425,050 to 1,425,472
_1246
Pf1N1B4_1247 - transcriptional regulator, MerR family, at 1,425,555 to 1,425,905
_1247
Pf1N1B4_1248 - Probable short-chain dehydrogenase, at 1,425,931 to 1,426,617
_1248
Pf1N1B4_1249 - Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3), at 1,426,872 to 1,428,230
_1249
Position (kb)
1425
1426
1427 Strain fitness (log2 ratio)
-1
0
1
2 at 1424.968 kb on - strand at 1425.171 kb on + strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.213 kb on + strand, within Pf1N1B4_1246 at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.572 kb on - strand at 1425.644 kb on + strand, within Pf1N1B4_1247 at 1425.644 kb on + strand, within Pf1N1B4_1247 at 1425.696 kb on + strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.726 kb on - strand, within Pf1N1B4_1247 at 1425.896 kb on - strand at 1426.129 kb on - strand, within Pf1N1B4_1248 at 1426.378 kb on + strand at 1426.378 kb on + strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.488 kb on + strand, within Pf1N1B4_1248 at 1426.488 kb on + strand, within Pf1N1B4_1248 at 1426.488 kb on + strand, within Pf1N1B4_1248 at 1426.558 kb on + strand at 1426.558 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.591 kb on + strand at 1426.592 kb on - strand at 1426.592 kb on - strand at 1426.592 kb on - strand at 1426.592 kb on - strand at 1427.079 kb on + strand, within Pf1N1B4_1249 at 1427.080 kb on - strand, within Pf1N1B4_1249 at 1427.080 kb on - strand, within Pf1N1B4_1249 at 1427.253 kb on + strand, within Pf1N1B4_1249 at 1427.253 kb on + strand, within Pf1N1B4_1249 at 1427.254 kb on - strand, within Pf1N1B4_1249 at 1427.518 kb on + strand, within Pf1N1B4_1249 at 1427.519 kb on - strand, within Pf1N1B4_1249 at 1427.519 kb on - strand, within Pf1N1B4_1249 at 1427.546 kb on + strand, within Pf1N1B4_1249 at 1427.557 kb on + strand, within Pf1N1B4_1249 at 1427.558 kb on - strand, within Pf1N1B4_1249 at 1427.558 kb on - strand, within Pf1N1B4_1249
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,424,968 - +0.5 1,425,171 + Pf1N1B4_1246 0.29 +1.1 1,425,172 - Pf1N1B4_1246 0.29 +0.8 1,425,172 - Pf1N1B4_1246 0.29 +0.7 1,425,172 - Pf1N1B4_1246 0.29 +2.3 1,425,213 + Pf1N1B4_1246 0.39 +1.1 1,425,520 + -0.5 1,425,520 + +1.1 1,425,520 + -0.3 1,425,520 + +1.1 1,425,520 + +1.5 1,425,520 + +0.3 1,425,521 - +0.2 1,425,521 - +1.3 1,425,521 - +0.1 1,425,521 - +0.1 1,425,521 - -0.0 1,425,572 - -0.4 1,425,644 + Pf1N1B4_1247 0.25 +0.3 1,425,644 + Pf1N1B4_1247 0.25 -0.5 1,425,696 + Pf1N1B4_1247 0.40 +1.3 1,425,697 - Pf1N1B4_1247 0.40 +0.9 1,425,697 - Pf1N1B4_1247 0.40 -0.1 1,425,697 - Pf1N1B4_1247 0.40 +0.5 1,425,697 - Pf1N1B4_1247 0.40 -0.3 1,425,697 - Pf1N1B4_1247 0.40 +0.7 1,425,697 - Pf1N1B4_1247 0.40 -0.3 1,425,697 - Pf1N1B4_1247 0.40 +0.3 1,425,697 - Pf1N1B4_1247 0.40 -1.7 1,425,726 - Pf1N1B4_1247 0.49 -1.6 1,425,896 - +0.1 1,426,129 - Pf1N1B4_1248 0.29 -0.2 1,426,378 + +1.1 1,426,378 + Pf1N1B4_1248 0.65 -0.2 1,426,379 - Pf1N1B4_1248 0.65 -0.4 1,426,379 - Pf1N1B4_1248 0.65 +2.1 1,426,379 - Pf1N1B4_1248 0.65 -0.7 1,426,379 - Pf1N1B4_1248 0.65 -1.6 1,426,488 + Pf1N1B4_1248 0.81 -1.5 1,426,488 + Pf1N1B4_1248 0.81 -0.9 1,426,488 + Pf1N1B4_1248 0.81 -1.5 1,426,558 + +0.4 1,426,558 + -0.5 1,426,591 + +1.5 1,426,591 + -0.6 1,426,591 + -0.5 1,426,591 + +0.1 1,426,591 + +0.1 1,426,592 - -1.5 1,426,592 - +0.1 1,426,592 - +0.1 1,426,592 - -0.6 1,427,079 + Pf1N1B4_1249 0.15 +0.1 1,427,080 - Pf1N1B4_1249 0.15 -0.3 1,427,080 - Pf1N1B4_1249 0.15 -0.9 1,427,253 + Pf1N1B4_1249 0.28 +0.7 1,427,253 + Pf1N1B4_1249 0.28 -0.8 1,427,254 - Pf1N1B4_1249 0.28 +0.9 1,427,518 + Pf1N1B4_1249 0.48 +0.4 1,427,519 - Pf1N1B4_1249 0.48 +0.6 1,427,519 - Pf1N1B4_1249 0.48 +1.3 1,427,546 + Pf1N1B4_1249 0.50 +1.3 1,427,557 + Pf1N1B4_1249 0.50 +1.5 1,427,558 - Pf1N1B4_1249 0.50 +0.7 1,427,558 - Pf1N1B4_1249 0.50 +0.4
Or see this region's nucleotide sequence