Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1234 and Pf1N1B4_1235 overlap by 4 nucleotides Pf1N1B4_1235 and Pf1N1B4_1236 overlap by 4 nucleotides
Pf1N1B4_1234 - Exodeoxyribonuclease V alpha chain (EC 3.1.11.5), at 1,400,057 to 1,402,165
_1234
Pf1N1B4_1235 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 1,402,162 to 1,405,851
_1235
Pf1N1B4_1236 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 1,405,848 to 1,409,300
_1236
Position (kb)
1402
1403
1404
1405
1406 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1401.209 kb on + strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.658 kb on + strand, within Pf1N1B4_1234 at 1401.697 kb on - strand, within Pf1N1B4_1234 at 1401.697 kb on - strand, within Pf1N1B4_1234 at 1402.172 kb on - strand at 1402.172 kb on - strand at 1402.269 kb on - strand at 1402.293 kb on + strand at 1402.311 kb on - strand at 1402.311 kb on - strand at 1402.311 kb on - strand at 1402.389 kb on - strand at 1402.389 kb on - strand at 1402.397 kb on + strand at 1402.398 kb on - strand at 1402.398 kb on - strand at 1402.452 kb on - strand at 1402.499 kb on + strand at 1402.499 kb on + strand at 1402.500 kb on - strand at 1402.602 kb on - strand at 1402.602 kb on - strand, within Pf1N1B4_1235 at 1403.174 kb on + strand, within Pf1N1B4_1235 at 1403.265 kb on - strand, within Pf1N1B4_1235 at 1403.265 kb on - strand, within Pf1N1B4_1235 at 1403.366 kb on + strand, within Pf1N1B4_1235 at 1403.469 kb on - strand, within Pf1N1B4_1235 at 1403.471 kb on + strand, within Pf1N1B4_1235 at 1403.472 kb on - strand, within Pf1N1B4_1235 at 1403.557 kb on - strand, within Pf1N1B4_1235 at 1404.137 kb on - strand, within Pf1N1B4_1235 at 1404.533 kb on + strand, within Pf1N1B4_1235 at 1404.534 kb on - strand, within Pf1N1B4_1235 at 1404.537 kb on + strand at 1404.537 kb on + strand at 1404.538 kb on - strand, within Pf1N1B4_1235 at 1404.539 kb on + strand, within Pf1N1B4_1235 at 1404.539 kb on + strand, within Pf1N1B4_1235 at 1404.539 kb on + strand, within Pf1N1B4_1235 at 1404.539 kb on + strand, within Pf1N1B4_1235 at 1404.539 kb on + strand, within Pf1N1B4_1235 at 1404.539 kb on + strand, within Pf1N1B4_1235 at 1404.539 kb on + strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.540 kb on - strand, within Pf1N1B4_1235 at 1404.563 kb on + strand, within Pf1N1B4_1235 at 1404.564 kb on - strand, within Pf1N1B4_1235 at 1404.564 kb on - strand, within Pf1N1B4_1235 at 1404.615 kb on + strand, within Pf1N1B4_1235 at 1404.998 kb on - strand, within Pf1N1B4_1235 at 1405.377 kb on + strand, within Pf1N1B4_1235 at 1405.629 kb on + strand at 1405.749 kb on - strand at 1405.761 kb on + strand at 1405.761 kb on + strand at 1405.761 kb on + strand at 1406.030 kb on - strand at 1406.030 kb on - strand at 1406.098 kb on - strand at 1406.225 kb on + strand, within Pf1N1B4_1236 at 1406.403 kb on + strand, within Pf1N1B4_1236 at 1406.580 kb on + strand, within Pf1N1B4_1236 at 1406.739 kb on + strand, within Pf1N1B4_1236 at 1406.740 kb on - strand, within Pf1N1B4_1236 at 1406.797 kb on + strand, within Pf1N1B4_1236
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,401,209 + Pf1N1B4_1234 0.55 -1.3 1,401,210 - Pf1N1B4_1234 0.55 +1.1 1,401,210 - Pf1N1B4_1234 0.55 -0.6 1,401,210 - Pf1N1B4_1234 0.55 +1.1 1,401,210 - Pf1N1B4_1234 0.55 -2.4 1,401,658 + Pf1N1B4_1234 0.76 -0.9 1,401,697 - Pf1N1B4_1234 0.78 +0.1 1,401,697 - Pf1N1B4_1234 0.78 -0.5 1,402,172 - +1.0 1,402,172 - +0.1 1,402,269 - -0.5 1,402,293 + +1.8 1,402,311 - -0.2 1,402,311 - -0.4 1,402,311 - +0.1 1,402,389 - -1.3 1,402,389 - +1.1 1,402,397 + +0.5 1,402,398 - +0.6 1,402,398 - -0.2 1,402,452 - +0.7 1,402,499 + -2.3 1,402,499 + -0.5 1,402,500 - +1.1 1,402,602 - -0.5 1,402,602 - Pf1N1B4_1235 0.12 -0.5 1,403,174 + Pf1N1B4_1235 0.27 -1.3 1,403,265 - Pf1N1B4_1235 0.30 +1.1 1,403,265 - Pf1N1B4_1235 0.30 -0.7 1,403,366 + Pf1N1B4_1235 0.33 -0.9 1,403,469 - Pf1N1B4_1235 0.35 +0.1 1,403,471 + Pf1N1B4_1235 0.35 -0.3 1,403,472 - Pf1N1B4_1235 0.36 -1.3 1,403,557 - Pf1N1B4_1235 0.38 -1.5 1,404,137 - Pf1N1B4_1235 0.54 -0.3 1,404,533 + Pf1N1B4_1235 0.64 -1.3 1,404,534 - Pf1N1B4_1235 0.64 +0.5 1,404,537 + +0.1 1,404,537 + +1.1 1,404,538 - Pf1N1B4_1235 0.64 -0.9 1,404,539 + Pf1N1B4_1235 0.64 -1.9 1,404,539 + Pf1N1B4_1235 0.64 -0.9 1,404,539 + Pf1N1B4_1235 0.64 -1.3 1,404,539 + Pf1N1B4_1235 0.64 -0.2 1,404,539 + Pf1N1B4_1235 0.64 +0.3 1,404,539 + Pf1N1B4_1235 0.64 +0.1 1,404,539 + Pf1N1B4_1235 0.64 -2.3 1,404,540 - Pf1N1B4_1235 0.64 -1.7 1,404,540 - Pf1N1B4_1235 0.64 -1.3 1,404,540 - Pf1N1B4_1235 0.64 +0.1 1,404,540 - Pf1N1B4_1235 0.64 +1.1 1,404,540 - Pf1N1B4_1235 0.64 +1.1 1,404,540 - Pf1N1B4_1235 0.64 -0.7 1,404,540 - Pf1N1B4_1235 0.64 -0.9 1,404,540 - Pf1N1B4_1235 0.64 -1.4 1,404,563 + Pf1N1B4_1235 0.65 +0.1 1,404,564 - Pf1N1B4_1235 0.65 +1.4 1,404,564 - Pf1N1B4_1235 0.65 +1.6 1,404,615 + Pf1N1B4_1235 0.66 -0.6 1,404,998 - Pf1N1B4_1235 0.77 -0.5 1,405,377 + Pf1N1B4_1235 0.87 +1.1 1,405,629 + -1.0 1,405,749 - +1.4 1,405,761 + -1.3 1,405,761 + -0.9 1,405,761 + -0.7 1,406,030 - +1.1 1,406,030 - +0.7 1,406,098 - +0.1 1,406,225 + Pf1N1B4_1236 0.11 -3.0 1,406,403 + Pf1N1B4_1236 0.16 -1.7 1,406,580 + Pf1N1B4_1236 0.21 +0.7 1,406,739 + Pf1N1B4_1236 0.26 -0.5 1,406,740 - Pf1N1B4_1236 0.26 +0.7 1,406,797 + Pf1N1B4_1236 0.27 +1.7
Or see this region's nucleotide sequence