Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1235

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1234 and Pf1N1B4_1235 overlap by 4 nucleotidesPf1N1B4_1235 and Pf1N1B4_1236 overlap by 4 nucleotides Pf1N1B4_1234 - Exodeoxyribonuclease V alpha chain (EC 3.1.11.5), at 1,400,057 to 1,402,165 _1234 Pf1N1B4_1235 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 1,402,162 to 1,405,851 _1235 Pf1N1B4_1236 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 1,405,848 to 1,409,300 _1236 Position (kb) 1402 1403 1404 1405 1406Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1401.209 kb on + strand, within Pf1N1B4_1234at 1401.210 kb on - strand, within Pf1N1B4_1234at 1401.210 kb on - strand, within Pf1N1B4_1234at 1401.210 kb on - strand, within Pf1N1B4_1234at 1401.210 kb on - strand, within Pf1N1B4_1234at 1401.658 kb on + strand, within Pf1N1B4_1234at 1401.697 kb on - strand, within Pf1N1B4_1234at 1401.697 kb on - strand, within Pf1N1B4_1234at 1402.172 kb on - strandat 1402.172 kb on - strandat 1402.269 kb on - strandat 1402.293 kb on + strandat 1402.311 kb on - strandat 1402.311 kb on - strandat 1402.311 kb on - strandat 1402.389 kb on - strandat 1402.389 kb on - strandat 1402.397 kb on + strandat 1402.398 kb on - strandat 1402.398 kb on - strandat 1402.452 kb on - strandat 1402.499 kb on + strandat 1402.499 kb on + strandat 1402.500 kb on - strandat 1402.602 kb on - strandat 1402.602 kb on - strand, within Pf1N1B4_1235at 1403.174 kb on + strand, within Pf1N1B4_1235at 1403.265 kb on - strand, within Pf1N1B4_1235at 1403.265 kb on - strand, within Pf1N1B4_1235at 1403.366 kb on + strand, within Pf1N1B4_1235at 1403.469 kb on - strand, within Pf1N1B4_1235at 1403.471 kb on + strand, within Pf1N1B4_1235at 1403.472 kb on - strand, within Pf1N1B4_1235at 1403.557 kb on - strand, within Pf1N1B4_1235at 1404.137 kb on - strand, within Pf1N1B4_1235at 1404.533 kb on + strand, within Pf1N1B4_1235at 1404.534 kb on - strand, within Pf1N1B4_1235at 1404.537 kb on + strandat 1404.537 kb on + strandat 1404.538 kb on - strand, within Pf1N1B4_1235at 1404.539 kb on + strand, within Pf1N1B4_1235at 1404.539 kb on + strand, within Pf1N1B4_1235at 1404.539 kb on + strand, within Pf1N1B4_1235at 1404.539 kb on + strand, within Pf1N1B4_1235at 1404.539 kb on + strand, within Pf1N1B4_1235at 1404.539 kb on + strand, within Pf1N1B4_1235at 1404.539 kb on + strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.540 kb on - strand, within Pf1N1B4_1235at 1404.563 kb on + strand, within Pf1N1B4_1235at 1404.564 kb on - strand, within Pf1N1B4_1235at 1404.564 kb on - strand, within Pf1N1B4_1235at 1404.615 kb on + strand, within Pf1N1B4_1235at 1404.998 kb on - strand, within Pf1N1B4_1235at 1405.377 kb on + strand, within Pf1N1B4_1235at 1405.629 kb on + strandat 1405.749 kb on - strandat 1405.761 kb on + strandat 1405.761 kb on + strandat 1405.761 kb on + strandat 1406.030 kb on - strandat 1406.030 kb on - strandat 1406.098 kb on - strandat 1406.225 kb on + strand, within Pf1N1B4_1236at 1406.403 kb on + strand, within Pf1N1B4_1236at 1406.580 kb on + strand, within Pf1N1B4_1236at 1406.739 kb on + strand, within Pf1N1B4_1236at 1406.740 kb on - strand, within Pf1N1B4_1236at 1406.797 kb on + strand, within Pf1N1B4_1236

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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1,401,209 + Pf1N1B4_1234 0.55 -1.3
1,401,210 - Pf1N1B4_1234 0.55 +1.1
1,401,210 - Pf1N1B4_1234 0.55 -0.6
1,401,210 - Pf1N1B4_1234 0.55 +1.1
1,401,210 - Pf1N1B4_1234 0.55 -2.4
1,401,658 + Pf1N1B4_1234 0.76 -0.9
1,401,697 - Pf1N1B4_1234 0.78 +0.1
1,401,697 - Pf1N1B4_1234 0.78 -0.5
1,402,172 - +1.0
1,402,172 - +0.1
1,402,269 - -0.5
1,402,293 + +1.8
1,402,311 - -0.2
1,402,311 - -0.4
1,402,311 - +0.1
1,402,389 - -1.3
1,402,389 - +1.1
1,402,397 + +0.5
1,402,398 - +0.6
1,402,398 - -0.2
1,402,452 - +0.7
1,402,499 + -2.3
1,402,499 + -0.5
1,402,500 - +1.1
1,402,602 - -0.5
1,402,602 - Pf1N1B4_1235 0.12 -0.5
1,403,174 + Pf1N1B4_1235 0.27 -1.3
1,403,265 - Pf1N1B4_1235 0.30 +1.1
1,403,265 - Pf1N1B4_1235 0.30 -0.7
1,403,366 + Pf1N1B4_1235 0.33 -0.9
1,403,469 - Pf1N1B4_1235 0.35 +0.1
1,403,471 + Pf1N1B4_1235 0.35 -0.3
1,403,472 - Pf1N1B4_1235 0.36 -1.3
1,403,557 - Pf1N1B4_1235 0.38 -1.5
1,404,137 - Pf1N1B4_1235 0.54 -0.3
1,404,533 + Pf1N1B4_1235 0.64 -1.3
1,404,534 - Pf1N1B4_1235 0.64 +0.5
1,404,537 + +0.1
1,404,537 + +1.1
1,404,538 - Pf1N1B4_1235 0.64 -0.9
1,404,539 + Pf1N1B4_1235 0.64 -1.9
1,404,539 + Pf1N1B4_1235 0.64 -0.9
1,404,539 + Pf1N1B4_1235 0.64 -1.3
1,404,539 + Pf1N1B4_1235 0.64 -0.2
1,404,539 + Pf1N1B4_1235 0.64 +0.3
1,404,539 + Pf1N1B4_1235 0.64 +0.1
1,404,539 + Pf1N1B4_1235 0.64 -2.3
1,404,540 - Pf1N1B4_1235 0.64 -1.7
1,404,540 - Pf1N1B4_1235 0.64 -1.3
1,404,540 - Pf1N1B4_1235 0.64 +0.1
1,404,540 - Pf1N1B4_1235 0.64 +1.1
1,404,540 - Pf1N1B4_1235 0.64 +1.1
1,404,540 - Pf1N1B4_1235 0.64 -0.7
1,404,540 - Pf1N1B4_1235 0.64 -0.9
1,404,540 - Pf1N1B4_1235 0.64 -1.4
1,404,563 + Pf1N1B4_1235 0.65 +0.1
1,404,564 - Pf1N1B4_1235 0.65 +1.4
1,404,564 - Pf1N1B4_1235 0.65 +1.6
1,404,615 + Pf1N1B4_1235 0.66 -0.6
1,404,998 - Pf1N1B4_1235 0.77 -0.5
1,405,377 + Pf1N1B4_1235 0.87 +1.1
1,405,629 + -1.0
1,405,749 - +1.4
1,405,761 + -1.3
1,405,761 + -0.9
1,405,761 + -0.7
1,406,030 - +1.1
1,406,030 - +0.7
1,406,098 - +0.1
1,406,225 + Pf1N1B4_1236 0.11 -3.0
1,406,403 + Pf1N1B4_1236 0.16 -1.7
1,406,580 + Pf1N1B4_1236 0.21 +0.7
1,406,739 + Pf1N1B4_1236 0.26 -0.5
1,406,740 - Pf1N1B4_1236 0.26 +0.7
1,406,797 + Pf1N1B4_1236 0.27 +1.7

Or see this region's nucleotide sequence