Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1209 and Pf1N1B4_1210 overlap by 11 nucleotides Pf1N1B4_1210 and Pf1N1B4_1211 are separated by 234 nucleotides Pf1N1B4_1211 and Pf1N1B4_1212 are separated by 418 nucleotides Pf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotides
Pf1N1B4_1209 - FIG00959330: hypothetical protein, at 1,372,393 to 1,372,737
_1209
Pf1N1B4_1210 - membrane protein, putative, at 1,372,727 to 1,373,491
_1210
Pf1N1B4_1211 - Hydrogen peroxide-inducible genes activator, at 1,373,726 to 1,374,658
_1211
Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379
_1212
Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739
_1213
Position (kb)
1373
1374
1375 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.760 kb on - strand at 1372.778 kb on + strand at 1372.779 kb on - strand at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.135 kb on + strand, within Pf1N1B4_1210 at 1373.136 kb on - strand, within Pf1N1B4_1210 at 1373.441 kb on + strand at 1373.469 kb on + strand at 1373.470 kb on - strand at 1373.485 kb on + strand at 1373.486 kb on - strand at 1373.486 kb on - strand at 1373.498 kb on - strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.535 kb on - strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.756 kb on + strand at 1373.757 kb on - strand at 1373.757 kb on - strand at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.724 kb on + strand at 1374.724 kb on + strand at 1374.725 kb on - strand at 1374.731 kb on - strand at 1374.737 kb on + strand at 1374.737 kb on + strand at 1374.738 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.766 kb on + strand at 1374.767 kb on - strand at 1374.768 kb on + strand at 1374.847 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.872 kb on + strand at 1374.872 kb on + strand at 1374.873 kb on - strand at 1374.873 kb on - strand at 1374.897 kb on - strand at 1375.320 kb on + strand, within Pf1N1B4_1212 at 1375.321 kb on - strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.469 kb on - strand at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.495 kb on + strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.514 kb on + strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.547 kb on + strand, within Pf1N1B4_1213
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,372,759 + +1.8 1,372,759 + -0.0 1,372,759 + -0.4 1,372,759 + -0.9 1,372,759 + +0.3 1,372,759 + +1.0 1,372,760 - +0.0 1,372,778 + +0.7 1,372,779 - +1.6 1,372,998 + Pf1N1B4_1210 0.35 +0.7 1,372,998 + Pf1N1B4_1210 0.35 -2.0 1,372,999 - Pf1N1B4_1210 0.36 +0.6 1,372,999 - Pf1N1B4_1210 0.36 +0.5 1,372,999 - Pf1N1B4_1210 0.36 +0.5 1,373,133 + Pf1N1B4_1210 0.53 +0.0 1,373,133 + Pf1N1B4_1210 0.53 +0.0 1,373,134 - Pf1N1B4_1210 0.53 +1.5 1,373,134 - Pf1N1B4_1210 0.53 +1.5 1,373,134 - Pf1N1B4_1210 0.53 -1.7 1,373,134 - Pf1N1B4_1210 0.53 +0.8 1,373,134 - Pf1N1B4_1210 0.53 +0.4 1,373,134 - Pf1N1B4_1210 0.53 -1.8 1,373,134 - -1.0 1,373,134 - Pf1N1B4_1210 0.53 +0.4 1,373,134 - Pf1N1B4_1210 0.53 +0.5 1,373,135 + Pf1N1B4_1210 0.53 +0.5 1,373,136 - Pf1N1B4_1210 0.53 -2.1 1,373,441 + -1.5 1,373,469 + +1.0 1,373,470 - -1.3 1,373,485 + -0.2 1,373,486 - -0.2 1,373,486 - -0.5 1,373,498 - -0.3 1,373,534 + -1.0 1,373,534 + -0.1 1,373,534 + +0.7 1,373,535 - +0.3 1,373,536 + -1.3 1,373,536 + +0.0 1,373,536 + +0.0 1,373,756 + +0.6 1,373,757 - -0.5 1,373,757 - +0.2 1,374,026 - Pf1N1B4_1211 0.32 -0.7 1,374,026 - Pf1N1B4_1211 0.32 +0.0 1,374,045 - Pf1N1B4_1211 0.34 +0.1 1,374,045 - Pf1N1B4_1211 0.34 -0.5 1,374,273 + Pf1N1B4_1211 0.59 +0.2 1,374,273 + Pf1N1B4_1211 0.59 +0.0 1,374,302 + Pf1N1B4_1211 0.62 -0.5 1,374,302 + Pf1N1B4_1211 0.62 -0.4 1,374,303 - -0.5 1,374,303 - Pf1N1B4_1211 0.62 +0.0 1,374,303 - Pf1N1B4_1211 0.62 -0.2 1,374,303 - Pf1N1B4_1211 0.62 +0.4 1,374,303 - Pf1N1B4_1211 0.62 +0.4 1,374,303 - Pf1N1B4_1211 0.62 -1.4 1,374,303 - Pf1N1B4_1211 0.62 +0.2 1,374,303 - Pf1N1B4_1211 0.62 +0.7 1,374,303 - +2.0 1,374,303 - Pf1N1B4_1211 0.62 +1.0 1,374,338 + Pf1N1B4_1211 0.66 +0.3 1,374,338 + Pf1N1B4_1211 0.66 -0.3 1,374,651 + +1.0 1,374,651 + +1.0 1,374,651 + +1.0 1,374,652 - +0.6 1,374,652 - +1.4 1,374,652 - +1.4 1,374,652 - -1.3 1,374,724 + +0.0 1,374,724 + +0.0 1,374,725 - +0.6 1,374,731 - +1.0 1,374,737 + -0.7 1,374,737 + -0.2 1,374,738 - +0.0 1,374,760 - -0.2 1,374,760 - +0.0 1,374,760 - -0.3 1,374,760 - -0.5 1,374,760 - +0.0 1,374,763 + -1.3 1,374,763 + +0.0 1,374,763 + +0.3 1,374,763 + +1.3 1,374,764 - -0.5 1,374,764 - +1.0 1,374,764 - -0.5 1,374,764 - +0.0 1,374,766 + +0.0 1,374,767 - -0.2 1,374,768 + +0.3 1,374,847 + -0.8 1,374,852 + +0.5 1,374,852 + +0.3 1,374,852 + -1.0 1,374,852 + +2.4 1,374,852 + +0.2 1,374,853 - +1.5 1,374,853 - +0.3 1,374,853 - -1.4 1,374,853 - -2.0 1,374,853 - +0.3 1,374,853 - -0.4 1,374,853 - +0.2 1,374,853 - -1.3 1,374,853 - +0.0 1,374,872 + +0.5 1,374,872 + +0.3 1,374,873 - +0.0 1,374,873 - +0.3 1,374,897 - -0.3 1,375,320 + Pf1N1B4_1212 0.80 +0.7 1,375,321 - Pf1N1B4_1212 0.81 +0.6 1,375,335 + Pf1N1B4_1212 0.85 +0.6 1,375,335 + Pf1N1B4_1212 0.85 -0.1 1,375,335 + Pf1N1B4_1212 0.85 +0.0 1,375,335 + Pf1N1B4_1212 0.85 +0.6 1,375,335 + Pf1N1B4_1212 0.85 +1.0 1,375,335 + Pf1N1B4_1212 0.85 -0.1 1,375,336 - Pf1N1B4_1212 0.85 +1.4 1,375,336 - Pf1N1B4_1212 0.85 +1.0 1,375,469 - -0.7 1,375,494 - Pf1N1B4_1213 0.11 +0.9 1,375,494 - Pf1N1B4_1213 0.11 +0.5 1,375,495 + Pf1N1B4_1213 0.11 -1.3 1,375,496 - Pf1N1B4_1213 0.12 +0.0 1,375,496 - Pf1N1B4_1213 0.12 -0.4 1,375,502 + Pf1N1B4_1213 0.14 +0.5 1,375,502 + Pf1N1B4_1213 0.14 +0.0 1,375,503 - Pf1N1B4_1213 0.14 +0.6 1,375,503 - Pf1N1B4_1213 0.14 -0.3 1,375,503 - Pf1N1B4_1213 0.14 -0.3 1,375,503 - Pf1N1B4_1213 0.14 +0.0 1,375,503 - Pf1N1B4_1213 0.14 -2.2 1,375,514 + Pf1N1B4_1213 0.18 -0.3 1,375,515 - Pf1N1B4_1213 0.18 +0.0 1,375,515 - Pf1N1B4_1213 0.18 -1.0 1,375,515 - Pf1N1B4_1213 0.18 +0.7 1,375,515 - Pf1N1B4_1213 0.18 -0.1 1,375,547 + Pf1N1B4_1213 0.30 +0.0
Or see this region's nucleotide sequence