Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1086

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1085 and Pf1N1B4_1086 are separated by 66 nucleotidesPf1N1B4_1086 and Pf1N1B4_1087 are separated by 1 nucleotidesPf1N1B4_1087 and Pf1N1B4_1088 are separated by 27 nucleotides Pf1N1B4_1085 - Metal transporter, ZIP family, at 1,215,760 to 1,216,617 _1085 Pf1N1B4_1086 - Manganese superoxide dismutase (EC 1.15.1.1), at 1,216,684 to 1,217,295 _1086 Pf1N1B4_1087 - FOG: TPR repeat, at 1,217,297 to 1,217,737 _1087 Pf1N1B4_1088 - Fumarate hydratase class II (EC 4.2.1.2), at 1,217,765 to 1,219,141 _1088 Position (kb) 1216 1217 1218Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1215.697 kb on + strandat 1215.716 kb on + strandat 1215.717 kb on - strandat 1215.717 kb on - strandat 1215.717 kb on - strandat 1216.588 kb on + strandat 1216.653 kb on + strandat 1216.685 kb on + strandat 1216.685 kb on + strandat 1216.685 kb on + strandat 1216.686 kb on - strandat 1216.686 kb on - strandat 1216.739 kb on + strandat 1216.754 kb on + strand, within Pf1N1B4_1086at 1216.754 kb on + strand, within Pf1N1B4_1086at 1216.754 kb on + strand, within Pf1N1B4_1086at 1216.755 kb on - strand, within Pf1N1B4_1086at 1216.755 kb on - strand, within Pf1N1B4_1086at 1216.784 kb on + strand, within Pf1N1B4_1086at 1216.784 kb on + strand, within Pf1N1B4_1086at 1216.785 kb on - strand, within Pf1N1B4_1086at 1216.785 kb on - strand, within Pf1N1B4_1086at 1216.785 kb on - strand, within Pf1N1B4_1086at 1216.788 kb on - strand, within Pf1N1B4_1086at 1216.824 kb on + strand, within Pf1N1B4_1086at 1217.192 kb on + strand, within Pf1N1B4_1086at 1217.192 kb on + strand, within Pf1N1B4_1086at 1217.193 kb on - strand, within Pf1N1B4_1086at 1217.193 kb on - strand, within Pf1N1B4_1086at 1217.193 kb on - strand, within Pf1N1B4_1086at 1217.213 kb on + strand, within Pf1N1B4_1086at 1217.261 kb on + strandat 1217.261 kb on + strandat 1217.286 kb on + strandat 1217.286 kb on + strandat 1217.286 kb on + strandat 1217.287 kb on - strandat 1217.287 kb on - strandat 1217.315 kb on + strandat 1217.315 kb on + strandat 1217.316 kb on - strandat 1217.316 kb on - strandat 1217.316 kb on - strandat 1217.418 kb on + strand, within Pf1N1B4_1087at 1217.418 kb on + strandat 1217.418 kb on + strandat 1217.419 kb on - strand, within Pf1N1B4_1087at 1217.489 kb on + strand, within Pf1N1B4_1087at 1217.489 kb on + strand, within Pf1N1B4_1087at 1217.570 kb on - strand, within Pf1N1B4_1087at 1217.658 kb on + strand, within Pf1N1B4_1087at 1217.658 kb on + strand, within Pf1N1B4_1087at 1217.659 kb on - strand, within Pf1N1B4_1087at 1217.659 kb on - strand, within Pf1N1B4_1087at 1217.766 kb on + strandat 1217.767 kb on - strandat 1217.874 kb on + strandat 1217.874 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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1,215,697 + -0.0
1,215,716 + -1.0
1,215,717 - +1.0
1,215,717 - -0.0
1,215,717 - +1.5
1,216,588 + -0.2
1,216,653 + -1.6
1,216,685 + -1.4
1,216,685 + +0.1
1,216,685 + +0.5
1,216,686 - +0.4
1,216,686 - +1.2
1,216,739 + -1.8
1,216,754 + Pf1N1B4_1086 0.11 -2.2
1,216,754 + Pf1N1B4_1086 0.11 -0.8
1,216,754 + Pf1N1B4_1086 0.11 +0.2
1,216,755 - Pf1N1B4_1086 0.12 -1.0
1,216,755 - Pf1N1B4_1086 0.12 +0.2
1,216,784 + Pf1N1B4_1086 0.16 -2.2
1,216,784 + Pf1N1B4_1086 0.16 +0.2
1,216,785 - Pf1N1B4_1086 0.17 +0.2
1,216,785 - Pf1N1B4_1086 0.17 +1.0
1,216,785 - Pf1N1B4_1086 0.17 +1.8
1,216,788 - Pf1N1B4_1086 0.17 +0.4
1,216,824 + Pf1N1B4_1086 0.23 +0.1
1,217,192 + Pf1N1B4_1086 0.83 +0.2
1,217,192 + Pf1N1B4_1086 0.83 -0.0
1,217,193 - Pf1N1B4_1086 0.83 -0.1
1,217,193 - Pf1N1B4_1086 0.83 -0.0
1,217,193 - Pf1N1B4_1086 0.83 +3.0
1,217,213 + Pf1N1B4_1086 0.86 +0.4
1,217,261 + -0.4
1,217,261 + -0.5
1,217,286 + +0.4
1,217,286 + +0.2
1,217,286 + +1.0
1,217,287 - +1.0
1,217,287 - +1.6
1,217,315 + -1.6
1,217,315 + +0.4
1,217,316 - +1.0
1,217,316 - +0.4
1,217,316 - -0.0
1,217,418 + Pf1N1B4_1087 0.27 -1.2
1,217,418 + -0.4
1,217,418 + +2.0
1,217,419 - Pf1N1B4_1087 0.28 +0.2
1,217,489 + Pf1N1B4_1087 0.44 -1.0
1,217,489 + Pf1N1B4_1087 0.44 -0.0
1,217,570 - Pf1N1B4_1087 0.62 +0.3
1,217,658 + Pf1N1B4_1087 0.82 +0.6
1,217,658 + Pf1N1B4_1087 0.82 -1.0
1,217,659 - Pf1N1B4_1087 0.82 -1.4
1,217,659 - Pf1N1B4_1087 0.82 -0.4
1,217,766 + -0.6
1,217,767 - +0.5
1,217,874 + -0.4
1,217,874 + -1.4

Or see this region's nucleotide sequence