Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1050 and Pf1N1B4_1051 are separated by 226 nucleotides Pf1N1B4_1051 and Pf1N1B4_1052 are separated by 179 nucleotides Pf1N1B4_1052 and Pf1N1B4_1053 are separated by 40 nucleotides Pf1N1B4_1053 and Pf1N1B4_1054 are separated by 15 nucleotides
Pf1N1B4_1050 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 1,186,216 to 1,187,319
_1050
Pf1N1B4_1051 - Glutathione peroxidase family protein, at 1,187,546 to 1,187,974
_1051
Pf1N1B4_1052 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 1,188,154 to 1,188,639
_1052
Pf1N1B4_1053 - hypothetical protein, at 1,188,680 to 1,188,991
_1053
Pf1N1B4_1054 - Phosphate acetyltransferase (EC 2.3.1.8), at 1,189,007 to 1,189,840
_1054
Position (kb)
1188
1189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1187.189 kb on + strand, within Pf1N1B4_1050 at 1187.192 kb on + strand, within Pf1N1B4_1050 at 1187.192 kb on + strand, within Pf1N1B4_1050 at 1187.338 kb on + strand at 1187.338 kb on + strand at 1187.338 kb on + strand at 1187.339 kb on - strand at 1187.345 kb on + strand at 1187.346 kb on - strand at 1187.359 kb on + strand at 1187.389 kb on - strand at 1187.389 kb on - strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.397 kb on - strand at 1187.397 kb on - strand at 1187.397 kb on - strand at 1187.431 kb on - strand at 1187.475 kb on + strand at 1187.475 kb on + strand at 1187.476 kb on - strand at 1187.501 kb on + strand at 1187.599 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.710 kb on - strand, within Pf1N1B4_1051 at 1187.772 kb on + strand, within Pf1N1B4_1051 at 1187.772 kb on + strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.904 kb on + strand, within Pf1N1B4_1051 at 1187.905 kb on - strand, within Pf1N1B4_1051 at 1187.978 kb on + strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1188.100 kb on - strand at 1188.428 kb on + strand, within Pf1N1B4_1052 at 1188.429 kb on - strand, within Pf1N1B4_1052 at 1188.429 kb on - strand, within Pf1N1B4_1052 at 1188.454 kb on + strand, within Pf1N1B4_1052 at 1188.455 kb on - strand, within Pf1N1B4_1052 at 1188.455 kb on - strand, within Pf1N1B4_1052 at 1188.522 kb on + strand, within Pf1N1B4_1052 at 1188.539 kb on + strand, within Pf1N1B4_1052 at 1188.601 kb on - strand at 1188.601 kb on - strand at 1188.602 kb on + strand at 1188.603 kb on - strand at 1188.603 kb on - strand at 1188.621 kb on + strand at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.968 kb on + strand at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 1,187,189 + Pf1N1B4_1050 0.88 +2.0 1,187,192 + Pf1N1B4_1050 0.88 -0.3 1,187,192 + Pf1N1B4_1050 0.88 +0.2 1,187,338 + +0.8 1,187,338 + -0.6 1,187,338 + +1.5 1,187,339 - -0.4 1,187,345 + +2.3 1,187,346 - +0.8 1,187,359 + +2.0 1,187,389 - -0.0 1,187,389 - -2.2 1,187,396 + -1.0 1,187,396 + -0.0 1,187,396 + -0.0 1,187,396 + -1.2 1,187,396 + +1.1 1,187,396 + +0.4 1,187,397 - +1.0 1,187,397 - -0.6 1,187,397 - -0.5 1,187,431 - -0.3 1,187,475 + +0.3 1,187,475 + -0.0 1,187,476 - +2.5 1,187,501 + +1.2 1,187,599 + Pf1N1B4_1051 0.12 -1.9 1,187,709 + Pf1N1B4_1051 0.38 -0.6 1,187,709 + Pf1N1B4_1051 0.38 +1.0 1,187,709 + Pf1N1B4_1051 0.38 -0.4 1,187,710 - Pf1N1B4_1051 0.38 +0.4 1,187,772 + Pf1N1B4_1051 0.53 +1.0 1,187,772 + Pf1N1B4_1051 0.53 -1.4 1,187,773 - Pf1N1B4_1051 0.53 -0.3 1,187,773 - Pf1N1B4_1051 0.53 +1.3 1,187,773 - Pf1N1B4_1051 0.53 -1.0 1,187,874 + Pf1N1B4_1051 0.76 +0.5 1,187,874 + Pf1N1B4_1051 0.76 -1.4 1,187,874 + Pf1N1B4_1051 0.76 -0.0 1,187,874 + Pf1N1B4_1051 0.76 +0.5 1,187,874 + -0.4 1,187,875 - Pf1N1B4_1051 0.77 +0.6 1,187,875 - Pf1N1B4_1051 0.77 -2.0 1,187,875 - Pf1N1B4_1051 0.77 -1.4 1,187,875 - Pf1N1B4_1051 0.77 +0.2 1,187,875 - Pf1N1B4_1051 0.77 -0.6 1,187,875 - Pf1N1B4_1051 0.77 -1.6 1,187,875 - Pf1N1B4_1051 0.77 -0.0 1,187,904 + Pf1N1B4_1051 0.83 -1.3 1,187,905 - Pf1N1B4_1051 0.84 +0.7 1,187,978 + +1.7 1,187,979 - -1.2 1,187,979 - +1.3 1,187,979 - +0.5 1,187,979 - -0.4 1,187,979 - +0.1 1,188,100 - -2.4 1,188,428 + Pf1N1B4_1052 0.56 +1.0 1,188,429 - Pf1N1B4_1052 0.57 -1.5 1,188,429 - Pf1N1B4_1052 0.57 -0.0 1,188,454 + Pf1N1B4_1052 0.62 +1.0 1,188,455 - Pf1N1B4_1052 0.62 +1.0 1,188,455 - Pf1N1B4_1052 0.62 +0.4 1,188,522 + Pf1N1B4_1052 0.76 -0.9 1,188,539 + Pf1N1B4_1052 0.79 -0.3 1,188,601 - -0.5 1,188,601 - -0.6 1,188,602 + +0.4 1,188,603 - +0.7 1,188,603 - +1.0 1,188,621 + -0.6 1,188,871 + Pf1N1B4_1053 0.61 -0.6 1,188,871 + Pf1N1B4_1053 0.61 +2.0 1,188,968 + -0.6 1,189,283 + Pf1N1B4_1054 0.33 +1.0 1,189,283 + Pf1N1B4_1054 0.33 -0.9 1,189,352 + Pf1N1B4_1054 0.41 -0.0 1,189,352 + Pf1N1B4_1054 0.41 +1.0
Or see this region's nucleotide sequence