Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01730

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01725 and MMP_RS01730 are separated by 50 nucleotidesMMP_RS01730 and MMP_RS01735 are separated by 66 nucleotides MMP_RS01725: MMP_RS01725 - class I SAM-dependent methyltransferase family protein, at 319,643 to 320,671 _RS01725 MMP_RS01730: MMP_RS01730 - hypothetical protein, at 320,722 to 321,102 _RS01730 MMP_RS01735: MMP_RS01735 - type II glyceraldehyde-3-phosphate dehydrogenase, at 321,169 to 322,191 _RS01735 Position (kb) 320 321 322Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 319.786 kb on - strand, within MMP_RS01725at 319.831 kb on - strand, within MMP_RS01725at 319.871 kb on + strand, within MMP_RS01725at 319.985 kb on - strand, within MMP_RS01725at 319.999 kb on + strand, within MMP_RS01725at 320.173 kb on + strand, within MMP_RS01725at 320.432 kb on + strand, within MMP_RS01725at 320.584 kb on - strandat 320.594 kb on + strandat 320.650 kb on - strandat 320.695 kb on + strandat 320.696 kb on - strandat 320.697 kb on + strandat 320.698 kb on - strandat 320.698 kb on - strandat 320.709 kb on - strandat 320.734 kb on + strandat 320.743 kb on + strandat 320.844 kb on - strandat 321.020 kb on + strandat 321.023 kb on - strandat 321.024 kb on + strandat 321.308 kb on + strandat 321.319 kb on - strandat 321.951 kb on - strandat 321.952 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
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319,786 - MMP_RS01725 0.14 -3.0
319,831 - MMP_RS01725 0.18 -3.4
319,871 + MMP_RS01725 0.22 -2.9
319,985 - MMP_RS01725 0.33 -2.5
319,999 + MMP_RS01725 0.35 -1.5
320,173 + MMP_RS01725 0.52 -5.1
320,432 + MMP_RS01725 0.77 -4.4
320,584 - -5.2
320,594 + -3.8
320,650 - -3.1
320,695 + -1.0
320,696 - -0.1
320,697 + -0.6
320,698 - +0.1
320,698 - -0.5
320,709 - -0.9
320,734 + -0.5
320,743 + +2.4
320,844 - -4.1
321,020 + -3.3
321,023 - -3.7
321,024 + -2.7
321,308 + -3.3
321,319 - -2.3
321,951 - -2.4
321,952 + -2.6

Or see this region's nucleotide sequence