Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08935 and MMP_RS01420 are separated by 241 nucleotides MMP_RS01420 and MMP_RS01425 are separated by 229 nucleotides MMP_RS01425 and MMP_RS01430 are separated by 17 nucleotides
MMP_RS08935: MMP_RS08935 - hypothetical protein, at 263,080 to 263,229
_RS08935
MMP_RS01420: MMP_RS01420 - hypothetical protein, at 263,471 to 263,668
_RS01420
MMP_RS01425: MMP_RS01425 - tyrosine--tRNA ligase, at 263,898 to 264,827
_RS01425
MMP_RS01430: MMP_RS01430 - mechanosensitive ion channel family protein, at 264,845 to 265,900
_RS01430
Position (kb) 263
264
265 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 262.926 kb on - strand at 262.927 kb on + strand at 263.020 kb on - strand at 263.020 kb on - strand at 263.069 kb on - strand at 263.072 kb on - strand at 263.427 kb on + strand at 263.454 kb on - strand at 263.476 kb on + strand at 263.486 kb on - strand at 263.618 kb on - strand, within MMP_RS01420 at 263.621 kb on + strand, within MMP_RS01420 at 263.681 kb on + strand at 263.706 kb on - strand at 263.732 kb on - strand at 263.736 kb on + strand at 263.758 kb on - strand at 263.778 kb on + strand at 263.801 kb on + strand at 263.838 kb on + strand at 263.838 kb on + strand at 263.902 kb on - strand at 263.909 kb on - strand at 264.233 kb on - strand at 264.238 kb on - strand at 264.238 kb on - strand at 264.291 kb on + strand at 264.341 kb on + strand at 264.368 kb on + strand at 264.512 kb on - strand at 264.547 kb on - strand at 264.547 kb on - strand at 264.942 kb on + strand at 265.180 kb on + strand, within MMP_RS01430 at 265.222 kb on - strand, within MMP_RS01430 at 265.495 kb on - strand, within MMP_RS01430 at 265.501 kb on - strand, within MMP_RS01430 at 265.502 kb on + strand, within MMP_RS01430 at 265.534 kb on - strand, within MMP_RS01430 at 265.535 kb on + strand, within MMP_RS01430 at 265.602 kb on - strand, within MMP_RS01430 at 265.603 kb on + strand, within MMP_RS01430 at 265.648 kb on + strand, within MMP_RS01430
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 262,926 - -0.0 262,927 + +0.2 263,020 - +1.4 263,020 - -0.3 263,069 - -0.2 263,072 - -0.2 263,427 + +0.4 263,454 - +0.2 263,476 + +0.4 263,486 - -0.0 263,618 - MMP_RS01420 0.74 +0.3 263,621 + MMP_RS01420 0.76 +0.1 263,681 + +0.4 263,706 - +0.6 263,732 - +0.5 263,736 + +0.2 263,758 - -0.9 263,778 + +0.5 263,801 + -0.4 263,838 + -2.9 263,838 + -1.9 263,902 - -3.6 263,909 - -2.3 264,233 - -0.6 264,238 - -2.4 264,238 - -1.8 264,291 + -1.3 264,341 + -2.4 264,368 + -2.4 264,512 - -1.9 264,547 - -1.0 264,547 - -3.1 264,942 + +0.2 265,180 + MMP_RS01430 0.32 -1.6 265,222 - MMP_RS01430 0.36 -1.9 265,495 - MMP_RS01430 0.62 +0.8 265,501 - MMP_RS01430 0.62 +0.6 265,502 + MMP_RS01430 0.62 +0.3 265,534 - MMP_RS01430 0.65 -0.2 265,535 + MMP_RS01430 0.65 +0.8 265,602 - MMP_RS01430 0.72 +0.2 265,603 + MMP_RS01430 0.72 -0.3 265,648 + MMP_RS01430 0.76 +1.6
Or see this region's nucleotide sequence